JCDSA  Vol.5 No.1 , March 2015
Antioxidant, Collagen Synthesis Activity in Vitro and Clinical Test on Anti-Wrinkle Activity of Formulated Cream Containing Veronica officinalis Extract
Abstract: In this study, our objective was to evaluate the antioxidant, cytotoxicity and collagen synthesis activity in vitro and also to test the anti-wrinkle effect of formulated cream containing Veronica officinalis extract in vivo. Antioxidant evaluation was based on the scavenging activity of free radicles (DPPH) and procollagen type 1 protein (P1P) synthesis test was performed in fibroblast cell. Clinical anti-wrinkle activity was performed on female subjects in placebo-controlled trail. Verbascoside (an isolated compound) showed higher (IC50 value of 36.24 ± 1.81 μg/ml) free radicle inhibition activity but weaker collagen synthesis activity. The ethanolic extract showed good inhibition to DPPH free radicals and also showed a significant effect in collagen synthesis activity without cytotoxicity. In the in vivo study, treatment with the formulated cream (Scoti-Speedwell) for 56 days significantly reduced the percentage of wrinkle area and length with 18.0% and 16.05%, respectively. Overall, Veronica officinalis extract containing product (Scoti-SpeedwellTM) can be regarded as a potent anti-wrinkle agent in human skin.
Cite this paper: Lee, H. , Ghimeray, A. , Yim, J. and Chang, M. (2015) Antioxidant, Collagen Synthesis Activity in Vitro and Clinical Test on Anti-Wrinkle Activity of Formulated Cream Containing Veronica officinalis Extract. Journal of Cosmetics, Dermatological Sciences and Applications, 5, 45-51. doi: 10.4236/jcdsa.2015.51006.

[1]   Kim, H.H., Cho, S., Lee, S., Kim, K.H., Cho, K.H., Eun, H.C. and Chung, J.H. (2006) Photoprotective and Anti-Skin-Aging Effects of Eicosapentaenoic Acid in Human Skin in Vivo. Journal of Lipid Research, 47, 921-930.

[2]   Verschooten, L., Claerhout, S., Van Laethem, A., et al. (2006) New Strategies of Photoprotection. Photochemistry and Photobiology, 82, 1016-1023.


[4]   Carsten, G., Manuel, G.K., Barbara, S., Evi, S., Mathias, K., Irmgard, M., Martin, Z. and Roman, H. (2013) Traditionally Used Veronica officinalis Inhibits Proinflammatory Mediators via the NF-κB Signaling Pathway in a Human Lung Cell Line. Journal of Ethnophamacology, 145, 118-126.

[5]   Vogl, S., Picker, P., Mihaly-Bison, J., Fakhrudin, N., Atanasov, A.G., Heiss, E.H., Wawrosch, C., Reznicek, G., Dirsch, V.M., Saukel, J. and Kopp, B. (2013) Ethnopharmacological in Vitro Studies on Austria’s Folk Medicine—An Unexplored Lore in Vitro Anti-Inflammatory Activities of 71 Austrian Traditional Herbal Drugs. Journal of Ethnopharmacology, 149, 750-771.

[6]   Nikolova, M. (2011) Screening of Radical Scavenging Activity and Polyphenol Content of Bulgarian Plant Species. Pharmacognosy Research, 3, 256-259.

[7]   Ronsted, N., Gobel, E., Franzyk, H., Jensen, S.R. and Olsen, C.E. (2000) Chemotaxonomy of Plantago. Iridoid Glucosides and Caffeoyl Phenylethanoids Glycosides. Phytochemistry, 55, 337-348.

[8]   Schilling, G., Huegel, M. and Mayer, W. (1982) Verbascoside and Isoverbascoside from Paulownia tomentosa Steud. Zeitschrift für Naturforschung B, 37, 1633-1635.

[9]   Mosmann, T. (1983) Rapid Colorimetric Assay for Cellular Growth and Survival: Application to Proliferation and Cytotoxicity Assays. Journal of Immunological Methods, 65, 55-63.

[10]   Tanayama, N. and Terao, T. (1992) Relationship of Serum Levels of Pro-Type I Collagen Peptide, Pro-Type III Collagen Peptide and Type IV 7S Collagen with Cervical Maturation. Gynecologic and Obstetric Investigation, 34, 24-26.

[11]   Braca, A., Tommasi, N.D., Bari, L.D., et al. (2001) Antioxidant Principles from Bauhinia Terapotensis. Journal of Natural Products, 64, 892-895.

[12]   Wagner, H. (2006) Multitarget Therapy—The Future of Treatment for More than Just Functional Dyspepsia. Phytomed, 13, 122-129.

[13]   Williamson, E.M. (2001) Synergy and Other Interactions in Phytomedicine. Phytomed, 8, 401-409.

[14]   Tanaka, T., Okada, T., Konishi, H., et al. (1993) The Effect of Reactive Oxygen Species on the Biosynthesis of Collagen and Glycosaminoglycans in Cultures Human Dermal Fibroblasts. Archives of Dermatological Research, 285, 352-255.