Distribution and Detection of Cowpea Viruses Infecting Cowpea in Uganda

Robert  Omadi; John  Obuo; Martin  Orawu

doi:10.4236/ajps.2015.65062

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AJPS Vol.6 No.5 , March 2015

Distribution and Detection of Cowpea Viruses Infecting Cowpea in Uganda

Martin Orawu^*, John Obuo, Robert Omadi

Abstract: Cowpea viruses are economically important diseases of cowpea in the major growing areas of Uganda and have inflicted negatively on food security, nutrition and income for many households. The objective of the study was to determine the prevalence and identity of viruses infecting cowpea in the growing districts of Uganda. Surveys were conducted in the field of grown cowpea, and virus symptoms were recorded on the basis of virus incidence and severity in the districts of Apac, Lira, Pader and Kumi. Symptomatic virus leaf samples were also collected from the same fields surveyed for serological test for detection of virus types using antibodies to cowpea aphid-borne mosaic virus (CABMV), cowpea mild mottle virus (CPMMV), cowpea severe mosaic virus (CPSMV), cowpea mosaic virus (CPMV), cowpea chlorotic mottle virus (CPCMV), cucumber mosaic virus (CMV) and cowpea chlorotic mosaic virus (CCMV) in double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA). Results showed that there was the highest percentage of incidence and severity in Kumi (85.4% and 13.8%, respectively) with the lowest percentage of incidence and severity observed in Apac (55.9% and 4.7%, respectively). Serological test revealed the presence of CABMV, CPMMV, CPSMV and CMV in the leaf samples as the major virus types in the surveyed districts. However, CPCMV, CPMV and CCMV were not detected in the leaf samples surveyed in the cowpea growing districts. Serological test also revealed that among the virus types detected, single and multiple virus types occurred in the plant samples. The highest single virus type occurring in the plant samples was CPMMV (11.6%) and the lowest was CABMV (1.8%). The virus types namely CMV, CABMV, CPMMV and CPSMV occurred in combinations with proportion of 7.1% in the infected plant samples. Therefore, this information obtained on the virus types provides an opportunity for breeders to develop cowpea variety with multiple resistance genes to control several virus types infecting cowpea.

Keywords: Cowpea, Incidence, Serology, Virus Types

Cite this paper: Orawu, M. , Obuo, J. and Omadi, R. (2015) Distribution and Detection of Cowpea Viruses Infecting Cowpea in Uganda. American Journal of Plant Sciences, 6, 574-581. doi: 10.4236/ajps.2015.65062.

References

[1] Ehlers, J.D. and Hall, A.E. (1997) Cowpea (Vigna unguiculata (L.) Walp.). Field Crops Research, 53, 187-204.
http://dx.doi.org/10.1016/S0378-4290(97)00031-2

[2] FAO (1997) Production Yearbook. Food and Agriculture Organisation of the United Nations, Rome, 98.

[3] Orawu, M. and Obuo, J.P. (2008) Baseline Survey on Cowpea Production, Utilisation and Constraints in Northern and Eastern Regions of Uganda. Technical Report, Grain Legume Improvement Program, Serere, No. 3, 13.

[4] Sakai, F., Dawson, J.R.O. and Watts, J.W. (1983) Interference in Infections of Tobacco Protoplasts with Two Bromoviruses. Journal of General Virolology, 64, 1347-1354.
http://dx.doi.org/10.1099/0022-1317-64-6-1347

[5] Tarawali, S.A., Smith, J.W., Hiernaux, P., Singh, B.B., Gupta, S.C., Tabo, R., Harris, F., Nokoe, S., Fernandez-Rivera, S. and Bationo, A. (2000) Integrated Natural Resource Management-Putting Livestock in the Picture. Integrated Natural Resource Management Meeting, Penang.

[6] Byoung-Cheorl, K., Inhwa, Y. and Molly, M.J. (2005) Genetics of Plant Virus Resistance. Annual Review of Phytopatholology, 43, 581-621.
http://dx.doi.org/10.1146/annurev.phyto.43.011205.141140

[7] Alegbejo, M.D. and Kashina, B.D. (2001) Status of Legume Viruses in Nigeria. Journal of Sustainable Agriculture, 18, 55-69.
http://dx.doi.org/10.1300/J064v18n01_05

[8] Vance, V.B., Berger, P.H., Carrington, J.C., Hunt, A.G. and Shi, X.M. (1995) Five Proximal Potyviral Sequence Mediates Potato Virus X/Potyviral Synergistic Disease in Transgenic Tobacco. Virology, 206, 583-590.
http://dx.doi.org/10.1016/S0042-6822(95)80075-1

[9] Hampton, R.O. (1983) Seed-Borne Viruses in Crop Germplasm Resources: Disease Dissemination Risks and Germplasm-Reclamation Technology. Seed Science and Technology, 11, 525-546.

[10] Catalina, G.L. and Elena-Cocuta, B. (2012) Validation of DAS-ELISA Results for the Detection of Grapevine Fleck Virus. Journal of Horticulture, Forestry and Biotechnology, 16, 57-61.

[11] Boscia, D., Digiaro, M., Fresno, J., Greif, C., Grenan, S., Kassemeyer, H.H., Prota, V.A. and De Sequueira, O.A. (1997) ELISA for the Detection and Identification of Grapevine Viruses. In: Walter, B., Ed., Sanitary Selection of the Grapevine, INRA Editions, Colmar (France), Les Colloques, 86, 129-155.

[12] Guatelli, J.C., Gingeras, T.R. and Richman, D.D. (1989) Nucleic Acid Amplification in Vitro Detection of Sequence with Low Copy Numbers and Application to Diagnosis of Human Immunodeficiency Virus Type 1 Infection. Clinical Microbiology Review, 2, 217-220.

[13] Ndunguru, J. and Jeremiah, S.C. (1999) Cassava Mosaic Disease Status in Bukoba and Karagwe Districts. Technical Report, OFDA CMD Project, IITA-ESARC, Kampala.

[14] Huguenot, C., Furneaux, M.T., Thottappilly, G., Rossel, H.W. and Hamilton, R.I. (1993) Evidence That Cowpea Aphid-Borne Mosaic and Blackeye Cowpea Mosaic Viruses Are Two Different Potyviruses. Journal of General Virology, 74, 335-340.
http://dx.doi.org/10.1099/0022-1317-74-3-335

[15] Shoyinka, S.A., Thottappilly, G., Adebayo, G.G. and Anno-Nyako, F.O. (1997) Survey on Cowpea Virus Incidence and Distribution in Nigeria. International Journal of Pest Management, 43, 127-132.
http://dx.doi.org/10.1080/096708797228816

[16] Vacke, J. (1983) Survival and Spreading of Wheat Dwarf Virus through the Seasonal Cycle. Proceedings of the 9th Czechoslovak Plant Protection Conference, Brno, 30 August-1 September 1983, 234-236.

[17] Bukvayová, N., Henselová, M., Vajciková, V. and Kormanová, T. (2006) Occurrence of Dwarf Virus of Winter Wheat and Barley in Several Regions of Slovakia during the Growing Seasons. Plant Soil Environment, 9, 392-401.

[18] Edema, R., Adipala, E. and Florini, D.A. (1997) Influence of Season and Cropping System on the Occurrence of Cowpea Diseases in Uganda. Plant Disease, 81, 465-468.
http://dx.doi.org/10.1094/PDIS.1997.81.5.465

[19] Duffus, J.E. (1971) Role of Weeds in the Incidence of Virus Diseases. Annual Review of Phytopathology, 9, 319-340.
http://dx.doi.org/10.1146/annurev.py.09.090171.001535

[20] Thresh, J.M. (1974) Temporal Patterns of Virus Spread. Annual Review of Phytopathology, 12, 111-128.
http://dx.doi.org/10.1146/annurev.py.12.090174.000551

[21] Atiri, G.I., Enobakhare, D.A. and Thottappilly, G. (1986) The Importance of Colonizing and Non-Colonizing Aphid Vectors in the Spread of Cowpea Aphid-Borne Mosaic Virus in Cowpea. Crop Protection, 5, 406-410.
http://dx.doi.org/10.1016/0261-2194(86)90073-6

[22] Wisler, G.C., Duffus, J.E., Liu, H.Y. and Li, R.H. (1998) Ecology and Epidemiology of Whitefly-Transmitted Closteroviruses. Plant Disease, 82, 270-280.
http://dx.doi.org/10.1094/PDIS.1998.82.3.270

[23] Fondong, V.N., Pita, J.S., Rey, M.E., de Kochko, A., Beachy, R.N. and Fauquent, C.M. (2000) Evidence of Synergism between African Cassava Mosaic Virus and a New Double-Recombinant Geminivirus Infecting Cassava in Cameroon. Journal of General Virology, 81, 287-297.