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 JSBS  Vol.5 No.1 , March 2015
Fuelization of Italian Ryegrass and Napier Grass through a Biological Treatment and Photocatalytic Reforming
Masahide Yasuda1*, Misriyani  2, Yuka Takenouchi1, Ryo Kurogi1, Shunsaku Uehara1, Tsutomu Shiragami1
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Abstract: Fuelization of Italian ryegrass and Napier grass was examined by the combination of biological treatments and photocatalytic reforming (photo-Reform). The alkali-pretreated Italian ryegrass and Napier grass were subjected to the enzymatic saccharification using cellulase and xylanase. Xylose and glucose were produced in 56.6% and 71.1% from Italian ryegrass and in 49.5% and 67.3% from Napier grass, respectively. Xylose and glucose were converted to hydrogen by the photo-Reform using a Pt-loaded titanium oxide (Pt/TiO2) under UV irradiation. Moreover, a low-moisture anhydrous ammonia (LMAA) pretreatment was performed for Italian ryegrass and Napier grass by keeping moist powdered biomass under NH3 gas atmosphere at room temperature for four weeks. The Italian ryegrass and Napier grass which were pretreated by LMAA method were subjected to simultaneous saccharification and fermentation (SSF) using a mixture of cellulase and xylanase as well as Saccharomyces cerevisiae in acetate buffer (pH 5.0). Ethanol and xylose were produced in 91.6% and 51.6% from LMAA-pretreated Italian ryegrass and 78.6% and 68.8% from Napier grass, respectively. After the evaporation of ethanol, xylose was converted to hydrogen by the photo-Reform. In the case of saccharification→photo-Reform, energy was recovered as hydrogen from the alkali-pretreated Italian ryegrass and Napier grass in 71.9% and 77.0% of energy recovery efficiency, respectively. In the case of SSF→photo-Reform, the energy was recovered in 82.7% and 77.2% as ethanol and hydrogen from the LMAA-pretreated Italian ryegrass and Napier grass, respectively.
Keywords: Italian Ryegrass, Napier Grass, Hydrolytic Enzyme, Saccharification, Simultaneous Saccharification
Cite this paper: Yasuda, M. ,  , M. , Takenouchi, Y. , Kurogi, R. , Uehara, S. and Shiragami, T. (2015) Fuelization of Italian Ryegrass and Napier Grass through a Biological Treatment and Photocatalytic Reforming. Journal of Sustainable Bioenergy Systems, 5, 1-9. doi: 10.4236/jsbs.2015.51001.
References

[1]   Ward, O.P. and Singh, A. (2002) Bioethanol Technology: Development and Perspectives. Advances in Applied Microbiology, 51, 53-80.
http://dx.doi.org/10.1016/S0065-2164(02)51001-7

[2]   Ohgren, K., Rudolf, A., Galbe, M. and Zacchi, G. (2006) Fuel Ethanol Production from Steam-Pretreated Corn Stover Using SSF at Higher Dry Matter Content. Biomass and Bioenergy, 30, 863-869.
http://dx.doi.org/10.1016/j.biombioe.2006.02.002

[3]   Balat, M. and Balat, H. (2009) Recent Trends in Global Production and Utilization of Bio-Ethanol Fuel. Applied Energy, 86, 2273-2282.
http://dx.doi.org/10.1016/j.apenergy.2009.03.015

[4]   Ni, M., Leung, D.Y., Leung, M.K. and Sumathy, K. (2006) An Overview of Hydrogen Production from Biomass. Fuel Processing Technology, 87, 461-472.
http://dx.doi.org/10.1016/j.fuproc.2005.11.003

[5]   Yasuda, M., Kurogi, R., Tsumagari, H., Shiragami, T. and Matsumoto, T. (2014) New Approach to Fuelization of Herbaceous Lignocelluloses through Simultaneous Saccharification and Fermentation Followed by Photocatalytic Reforming. Energies, 7, 4087-4097.
http://dx.doi.org/10.3390/en7074087

[6]   Fujishima, A., Rao, T.N. and Tryk, D.A. (2000) Titanium Dioxide Photocatalysis. Journal of Photochemistry and Photobiology C—Photochemistry Reviews, 1, 1-21.

[7]   Galinska, A. and Walendziewski, J. (2005) Photocatalytic Water Splitting over Pt-TiO2 in the Presence of Sacrificial Reagents. Energy and Fuels, 19, 1143-1147.
http://dx.doi.org/10.1021/ef0400619

[8]   Shiragami, T., Tomo, T., Matsumoto, T. and Yasuda, M. (2013) Structural Dependence of Alcoholic Sacrificial Agents on TiO2-Photocatalytic Hydrogen Evolution. Bulletin of the Chemical Society of Japan, 86, 382-389.
http://dx.doi.org/10.1246/bcsj.20120274

[9]   Shiragami, T., Tomo, T., Tsumagari, H., Ishii, Y. and Yasuda, M. (2012) Hydrogen Evolution from Napiergrass by the Combination of Biological Treatment and a Pt-Loaded TiO2-Photocatalytic Reaction. Catalyst, 2, 56-67.
http://dx.doi.org/10.3390/catal2010056

[10]   Yasuda, M., Takenouchi, Y., Nitta, Y., Ishii, Y. and Ohta, K. (2014) Italian Ryegrass (Lolium multiflorum Lam) as Potential Bio-Ethanol Resource. BioEnergy Research, under Review.

[11]   Yasuda, M., Nagai, H., Takeo, K., Ishii, Y. and Ohta, K. (2014) Bio-Ethanol Production through Simultaneous Saccharification and Co-Fermentation (SSCF) of a Low-Moisture Anhydrous Ammonia (LMAA)-Pretreated Napiegrass (Pennisetum purpureum Schumach). Springer Plus, 3, 333.
http://dx.doi.org/10.1186/2193-1801-3-333

[12]   Silverstein, R.A., Chen, T., Sharma-Shivappa, R.R., Boyette, M.D. and Osborne, J. (2007) A Comparison of Chemical Pretreatment Methods for Improving Saccharification of Cotton Stalks. Bioresource Technology, 98, 3000-3011.
http://dx.doi.org/10.1016/j.biortech.2006.10.022

[13]   Yoo, C.G., Nghiem, N.P., Hicks, K.B. and Kim, T.H. (2011) Pretreatment of Corn Stover by Low Moisture Anhydrous Ammonia (LMAA) Process. Bioresource Technology, 102, 10028-10034.
http://dx.doi.org/10.1016/j.biortech.2011.08.057

[14]   Yasuda, M., Takeo, K., Nagai, H., Uto, T., Yui, T., Matsumoto, T., Ishii, Y. and Ohta, K. (2013) Enhancement of Ethanol Production from Napiergrass (Pennisetum purpureum Schumach) by a Low-Moisture Anhydrous Ammonia Pretreatment. Journal of Sustainable Bioenergy Systems, 13, 179-185.
http://dx.doi.org/10.4236/jsbs.2013.33025

[15]   Sluiter, A., Hames, B., Ruiz, R., Scarlata, C., Sluiter, J., Templaton, D. and Crocker, D. (2010) Determination of Structural Carbohydrates and Lignin in Biomass. Technical Report (2010) NREL/TP-510-42618, National Renewable Energy Laboratory, Golden.
http://www.nrel.gov/biomass/analytical_procedures.html

[16]   Yasuda, M., Miura, A., Shiragami, T., Matsumoto, J., Kamei, I., Ishii, Y. and Ohta, K. (2012) Ethanol Production from Non-Pretreated Napiergrass through a Simultaneous Saccharification and Fermentation Process Followed by a Pentose Fermentation with Escherichia coli KO11. Journal of Bioscience and Bioengineering, 114, 188-192.
http://dx.doi.org/10.1016/j.jbiosc.2012.03.011

[17]   Kennedy III, J.C. and Datye, A.K. (1998) Photochemical Heterogeneous Oxidation of Ethanol over Pt/TiO2. Journal of Catalysis, 179, 375-389.
http://dx.doi.org/10.1006/jcat.1998.2242

[18]   Atkins, P.W. (1994) Physical Chemistry. 5th Edition, Oxford University Press, Oxford, 922-926.

[19]   Ribeiro Da Silva, M.A.V., Ribeiro Da Silva, M.D.M.C., Lobo Ferreira, A.I.M.C., Shi, Q., Woodfield, B.F. and Goldberg, R.N. (2013) Thermochemistry of α-D-xylose(cr). Journal of Chemical Thermodynamics, 58, 20-28.
http://dx.doi.org/10.1016/j.jct.2012.09.028

 
 
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