Endogenous Levels of Polyamines under Water-Deficit Stress during Cotton’s Reproductive Development

Cristiane  Pilon; Derrick M.  Oosterhuis; Dimitra A.  Loka

doi:10.4236/ajps.2015.62039

Dimitra A. Loka, Derrick M. Oosterhuis, Cristiane Pilon

Abstract: Polyamines, putrescine (PUT), spermidine (SPD) and spermine (SPM) are implicated in plants’ responses under conditions of abiotic stress. Previous research in other crops has indicated that polyamines and changes in their concentrations are associated with drought tolerance under conditions of water-deficit stress; however, no information exists on cotton (Gossypium hirsutum L.). Growth chamber experiments were conducted with two cotton cultivars differing in drought tolerance, ST5288B2F (drought-sensitive) and Siokra L23 (drought-tolerant) in order to investigate the distribution of free polyamines, the effect of water-deficit stress on the polyamine metabolism of cotton reproductive units and their subtending leaves as well as the possible relationship between polyamines and drought tolerance in cotton. Our results showed that cotton ovaries contained significantly higher levels of total free polyamines compared to their subtending leaves under both control and water stress conditions. Water-deficit stress significantly increased PUT concentrations in ST5288B2F, while SPM levels significantly decreased in Siokra L23. The results indicated that water-deficit stress significantly affected cotton polyamine metabolism in reproductive structures and their subtending leaves; however, no clear relationship between drought-tolerance and changes in polyamine accumulation was established. Further research is needed to elucidate the mechanism according to which water-deficit stress affects polyamine metabolism.

Keywords: Cotton, Gas Exchange, Polyamine Levels, Reproductive Units, Water-Deficit Stress

Cite this paper: Loka, D. , Oosterhuis, D. and Pilon, C. (2015) Endogenous Levels of Polyamines under Water-Deficit Stress during Cotton’s Reproductive Development. American Journal of Plant Sciences, 6, 344-354. doi: 10.4236/ajps.2015.62039.

References

[1] Kakkar, R.K. and Sawhney, V.P. (2002) Polyamine Research in Plants: A Changing Perspective. Physiologia Plantarum, 116, 281-292.
http://dx.doi.org/10.1034/j.1399-3054.2002.1160302.x

[2] Oosterhuis, D.M. and Loka, D. (2012) Polyamines and Cotton Flowering. In: Oosterhuis, D.M. and Cothren, J.T., Eds., Flowering and Fruiting of Cotton, Publ. Cotton Foundation, Memphis, 109-132

[3] Alcazar, R., Altabella, T., Marco, F., Bortolotti, C., Reymond, M., Koncz, C., Carrasco, P. and Tiburcio, A.F. (2010) Polyamines: Molecules with Regulatory Functions in Plant Abiotic Stress Tolerance. Planta, 231, 1237-1249.
http://dx.doi.org/10.1007/s00425-010-1130-0

[4] Yang, J., Zhang, J., Liu, K., Wang, Z. and Liu, L. (2007) Involvement of Polyamines in Drought Resistance of Rice. Journal of Experimental Botany, 58, 1545-1555.
http://dx.doi.org/10.1093/jxb/erm032

[5] Lazcano-Ferrat, I. and Lovatt, C.J. (1999) Relationship between Relative Water Content, Nitrogen Pools and Growth of Phaseolus vulgaris L. and P. acutifolius, A. Gray during Water Deficit. Crop Science, 39, 467-475.
http://dx.doi.org/10.2135/cropsci1999.0011183X0039000200028x

[6] Bibi, A.C., Oosterhuis, D.M. and Gonias, E.D. (2010) Exogenous Application of Putrescine Ameliorates the Effect of High Temperature in Gossypium hirsutum L. Flowers and Fruit Development. Journal of Agronomy and Crop Science, 196, 205-211.
http://dx.doi.org/10.1111/j.1439-037X.2009.00414.x

[7] Liu, H.P., Dong, B.H., Zhang, Y.Y., Liu, Z.P. and Liu, Y.L. (2004) Relationship between Osmotic Stress and the Levels of Free, Conjugated and Bound Polyamines in Leaves of Wheat Seedlings. Plant Science, 166, 1261-1267.
http://dx.doi.org/10.1016/j.plantsci.2003.12.039

[8] Kuehn, G.D., Rodriguez-Garay, B., Bagga, S. and Phillips, G.C. (1990) Novel Occurrence of Uncommon Polyamines in Higher Plants. Plant Physiology, 94, 855-857.
http://dx.doi.org/10.1104/pp.94.3.855

[9] Davidonis, G. (1995) Changes in Polyamine Distribution during Cotton Fiber and Seed Development. Journal of Plant Physiology, 145, 108-112.
http://dx.doi.org/10.1016/S0176-1617(11)81855-9

[10] Bibi, A, C., Ooosterhuis, D.M., Gonias, E.D. and Mattice, J.D. (2012) Nodal Distribution of Free Polyamines in Cotton Ovaries Determined by HPLC. Journal of Agricultural Science, 150, 365-372.
http://dx.doi.org/10.1017/S0021859611000633

[11] Loka, D. and Oosterhuis, D.M. (2012) Water Stress and Reproductive Development in Cotton. In: Oosterhuis, D.M. and Cothren, J.T., Eds., Flowering and Fruiting in Cotton, Publ. Cotton Foundation, Memphis, 51-57.

[12] Nepomuceno, A.L., Oosterhuis, D.M. and Stewart, J.M. (1998) Physiological Responses of Cotton Leaves and Roots to Water Deficit Induced by Polyethelene Glycol. Environmental and Experimental Botany, 40, 29-41.
http://dx.doi.org/10.1016/S0098-8472(98)00018-5

[13] Stiller, W.N., Read, J.J., Constable, G.A. and Reid, P.A. (2005) Selection for Water Use Efficiency Traits in a Cotton Breeding Program: Cultivar Differences. Crop Science, 45, 1107-1113.
http://dx.doi.org/10.2135/cropsci2004.0545

[14] Wheelus, A.D., Ritchie, G.L., Sexton, L. and Ford, J. (2009) Screening Water Stress in Multiple Cotton Varieties. Georgia Cotton Research and Extension Report, 78-82.

[15] Hanzawa, Y., Imai, A., Michael, A.J., Komeda, Y. and Takahashi, T. (2002) Characterization of the Spermidine Synthase-Related Gene Family in Arabidopsis thaliana. FEBS Letters, 527, 176-180.
http://dx.doi.org/10.1016/S0014-5793(02)03217-9

[16] Minocha, R., Majumdar, R. and Minocha, S.C. (2014) Polyamines and Abiotic Stress in Plants: A Complex Relationship. Frontiers Plant Science, 5, 1-17. http://dx.doi.org/10.3389/fpls.2014.00175

[17] Alabadi, D., Aguero, M.S., Perez-Amador, M.A. and Carbonell, J. (1996) Arginase, Arginine Decarboxylase, Ornithine Decarboxylase, and Polyamines in Tomato Ovaries. Plant Physiology, 112, 1237-1244.

[18] Bae, H., Kim, S.H., Kim, M.S., Sicher, R.C., Lary, D., Strem, M.D., Natarajan, S. and Bailey, B.A. (2008) The Drought Response of Theobroma cacao and the Regulation of Genes Involved in Polyamine Biosynthesis by Drought and Other Stresses. Plant Physiology and Biochemistry, 46, 174-188.
http://dx.doi.org/10.1016/j.plaphy.2007.10.014

[19] Yamaguchi, K., Takahashi, Y., Berberich, T., Imai, A., Takahashi, T., Michael, A.J. and Kusano, T. (2007) A Protective Role for the Polyamine Spermine against Drought Stress in Arabidopsis. Biochemical and Biophysical Research Communications, 352, 486-490. http://dx.doi.org/10.1016/j.bbrc.2006.11.041

[20] Nayyar, H., Kaur, S., Kumar, S., Singh, K.J. and Dhir, K.K. (2005) Involvement of Polyamines in the Contrasting Sensitivity of Chickpea (Cicer arietinum L.) and Soybean (Glycine max L. Merrill) to Water-Deficit Stress. Botanical Billboard Academia Sinica, 46, 333-338.

[21] Lefevre, I., Gratia, E. and Lutts, S. (2001) Discrimination between the Ionic and Osmotic Components of Salt Stress in Relation to Free Polyamine Level in Rice (Oryza sativa). Plant Science, 161, 943-952. http://dx.doi.org/10.1016/S0168-9452(01)00485-X

[22] Alcazar, R., Planas, J., Saxena, T., Zarza, X., Bortolotti, C., Cuevas, J., Bitrian, M., Tiburcio, A.F. and Altabella, T. (2010) Putrescine Accumulation Confers Drought Tolerance in Transgenic Arabidopsis Plants Over-Expressing the Homologous Arginine decarboxylase 2 Gene. Plant Physiology and Biochemistry, 48, 547-552. http://dx.doi.org/10.1016/j.plaphy.2010.02.002

[23] Pettigrew, W.T. (2004) Physiological Consequences of Moisture Deficit Stress in Cotton. Crop Science, 44, 1265-1272. http://dx.doi.org/10.2135/cropsci2004.1265

[24] Capell, T., Bassle, L. and Christou, P. (2004) Modulation of the Polyamine Biosynthetic Pathway in Trasngenic Rice Confers Tolerance to Drought Stress. Proceedings of the National Academy of Science of the USA, 101, 9909-9914. http://dx.doi.org/10.1073/pnas.0306974101

[25] Voloudakis, A.E., Kosmas, S.A., Tsakas, S., Eliopoulos, E., Loukas, M. and Kosmidou, K. (2002) Expression of Selected Drought-Related Genes and Physiological Response of Greek Cotton Varieties. Functional Plant Biology, 29, 1237-1245. http://dx.doi.org/10.1071/PP01253

[26] Farooq, M., Wahid, A. and Lee, D.J. (2008) Exogenous Applied Polyamines Increase Drought Tolerance of Rice by Improving Leaf Water Status, Photosynthesis, and Membrane Properties. Acta Physiologiae Plantarum, 31, 937-945.

[27] Liu, K., Fu, H., Bei, Q. and Luan, S. (2000) Inward Potassium Channel in Guard Cells as a Target of Polyamine Regulation of Stomatal Movements. Plant Physiology, 124, 1315-1325.
http://dx.doi.org/10.1104/pp.124.3.1315

[28] Zhang, R.H., Li, J., Guo, S.R. and Tezuka, T. (2009) Effects of Exogenous Putrescine on Gas Exchange Characteristics and Chlorophyll Fluorescence of NaCl-Stressed Cucumber Seedlings. Photosynthesis Research, 100, 155-162.
http://dx.doi.org/10.1007/s11120-009-9441-3

[29] Flores, H.E. and Galston, A.W. (1984) Osmotic-Stress Induced Accumulation in Cereal Leaves. I. Physiological Parameters of the Response. Plant Physiology, 75, 102-109.
http://dx.doi.org/10.1104/pp.75.1.102

[30] Pang, X.M., Zhang, Z.Y., Wen, X.P., Ban, Y. and Moriguchi, T. (2007) Polyamines, All-Purpose Players in Response to Environmental Stresses in Plants. Plant Stress, 1, 173-188.

[31] Capell, T., Campos, J.L. and Tiburcio, A.F. (1993) Antisenescence Properties of Guazatine in Osmotically Stressed Oat Leaves. Phytochemistry, 32, 785-788. http://dx.doi.org/10.1016/0031-9422(93)85205-6

[32] Besford, R.T., Richardson, C.M., Campos, J.L. and Tiburcio, A.F. (1993) Effect of Polyamines on Stabilization of Molecular Complexes in Thylakoid Membranes of Osmotically Stressed Oat Leaves. Planta, 189, 201-206.
http://dx.doi.org/10.1007/BF00195077

[33] Islam, M.A., Blake, T.J., Kocacinar, F. and Lada, R. (2003) Ambiol, Spermine and Aminoethoxyvinylglycine Prevent Water Stress and Protect Membrane in Pinus strobus under Drought. Trees Structure and Function, 17, 227-235.