OJOph  Vol.5 No.1 , February 2015
Effects of Cyclopentolate on Form Deprivation Myopia in Guinea Pigs
ABSTRACT
Purpose: To investigate the effects of intravitreal injection of cyclopentolate on form deprivation myopia in guinea pigs. Methods: Thirty-five guinea pigs at age of 3 weeks were randomly divided into 5 groups (n = 7 for each group): deprived, deprived plus saline, deprived plus cyclopentolate, normal control, and cyclopentolate group. Form deprivation was only performed in right eyes with translucent membranes for 4 weeks. Physiological saline and cyclopentolate were intravitreally injected into deprived eyes at four-day intervals. All the left eyes remained untreated as group control. Refraction was measured by retinoscopy after cycloplegia. The axial dimensions were measured by A-scan ultrasound. Subsequently, retinal histology was observed by light microscopy. Results: After 4 weeks of treatment, intravitreal injection of cyclopentolate significantly reduced the degree of myopia in the deprived eyes (from -3.92 D to -0.86 D, P < 0.001), and retarded the increase of vitreous chamber depth (from 3.83 ± 0.06 mm to 3.70 ± 0.05 mm, P < 0.001) and axial length (from 8.42 ± 0.04 mm to 8.30 ± 0.05 mm, P < 0.001) in the deprived eyes. Histological ex-amination revealed no evidence of retinal damage of eyes injected with physiological saline or cy-clopentolate compared with normal control eyes. Conclusions: Intravitreal administration of cy-clopentolate reduces axial elongation of the deprived eyes in guinea pigs. Further investigations are required to identify the optimal dose.

Cite this paper
Li, T. , Zhou, X. , Chen, Z. and Zhou, X. (2015) Effects of Cyclopentolate on Form Deprivation Myopia in Guinea Pigs. Open Journal of Ophthalmology, 5, 10-18. doi: 10.4236/ojoph.2015.51003.
References
[1]   Gee, S.S. and Tabbara, K.F. (1988) Increase in Ocular AXIAL Length in Patients with Corneal Opacification. Ophthalmology, 95, 1276-1278.
http://dx.doi.org/10.1016/S0161-6420(88)33035-6

[2]   Miller-Meeks, M.J., Bennett, S.R., Keech, R.V. and Blodi, C.F. (1990) Myopia Induced by Vitreous Hemorrhage. American Journal of Ophthalmology, 109, 199-203.
http://dx.doi.org/10.1016/S0002-9394(14)75987-2

[3]   Wallman, J., Turkel, J. and Trachtman, J. (1978) Extreme Myopia Produced by Modest Change in Early Visual experience. Science, 201, 1249-1251.
http://dx.doi.org/10.1126/science.694514

[4]   Siegwart, J.J. and Norton, T.T. (1998) The Susceptible Period for Deprivation-Induced Myopia in Tree Shrew. Vision Research, 38, 3505-3515.
http://dx.doi.org/10.1016/S0042-6989(98)00053-4

[5]   Lu, F., Zhou, X., Zhao, H., Wang, R., Jia, D., Jiang, L., et al. (2006) Axial Myopia Induced by a Monocularly-Deprived Facemask in Guinea Pigs: A Non-Invasive and Effective Model. Experimental Eye Research, 82, 628-636.
http://dx.doi.org/10.1016/j.exer.2005.09.001

[6]   Howlett, M.H. and McFadden, S.A. (2006) Form-Deprivation Myopia in the Guinea Pig (Cavia porcellus). Vision Research, 46, 267-283.
http://dx.doi.org/10.1016/j.visres.2005.06.036

[7]   Smith, E.R., Huang, J., Hung, L.F., Blasdel, T.L., Humbird, T.L. and Bockhorst, K.H. (2009) Hemiretinal form Deprivation: Evidence for Local Control of Eye Growth and Refractive Development in Infant Monkeys. Investigative Ophthalmology & Visual Science, 50, 5057-5069.
http://dx.doi.org/10.1167/iovs.08-3232

[8]   Huang, J., Hung, L.F., Ramamirtham, R., Blasdel, T.L., Humbird, T.L., Bockhorst, K.H., et al. (2009) Effects of Form Deprivation on Peripheral Refractions and Ocular Shape in Infant Rhesus Monkeys (Macaca mulatta). Investigative Ophthalmology & Visual Science, 50, 4033-4044.
http://dx.doi.org/10.1167/iovs.08-3162

[9]   Shen, W., Vijayan, M. and Sivak, J.G. (2005) Inducing Form-Deprivation Myopia in fish. Investigative Ophthalmology & Visual Science, 46, 1797-1803.
http://dx.doi.org/10.1167/iovs.04-1318

[10]   Tejedor, J. and de la Villa, P. (2003) Refractive Changes Induced by Form Deprivation in the Mouse Eye. Investigative Ophthalmology & Visual Science, 44, 32-36.
http://dx.doi.org/10.1167/iovs.01-1171

[11]   Meng, W., Butterworth, J., Malecaze, F. and Calvas, P. (2011) Axial Length of Myopia: A Review of Current Research. Ophthalmologica, 225, 127-134.
http://dx.doi.org/10.1159/000317072

[12]   McBrien, N.A., Moghaddam, H.O. and Reeder, A.P. (1993) Atropine Reduces Experimental Myopia and Eye Enlargement via a Nonaccommodative Mechanism. Investigative Ophthalmology & Visual Science, 34, 205-215.

[13]   Leech, E.M., Cottriall, C.L. and McBrien, N.A. (1995) Pirenzepine Prevents form Deprivation Myopia in a Dose Dependent Manner. Ophthalmic and Physiological Optics, 15, 351-356.
http://dx.doi.org/10.1016/0275-5408(95)00074-N

[14]   Cottriall, C.L., McBrien, N.A., Annies, R. and Leech, E.M. (1999) Prevention of Form-Deprivation Myopia with Pirenzepine: A Study of Drug Delivery and Distribution. Ophthalmic and Physiological Optics, 19, 327-335.
http://dx.doi.org/10.1016/S0275-5408(98)00079-9

[15]   Cottriall, C.L., Truong, H.T. and McBrien, N.A. (2001) Inhibition of Myopia Development in Chicks Using Himbacine: A Role for M4 Receptors? Neuroreport, 12, 2453-2456.
http://dx.doi.org/10.1097/00001756-200108080-00033

[16]   Luft, W.A., Ming, Y. and Stell, W.K. (2003) Variable Effects of Previously Untested Muscarinic Receptor Antagonists on Experimental Myopia. Investigative Ophthalmology & Visual Science, 44, 1330-1338.
http://dx.doi.org/10.1167/iovs.02-0796

[17]   Chua, W.H., Balakrishnan, V., Chan, Y.H., Tong, L., Ling, Y., Quah, B.L., et al. (2006) Atropine for the Treatment of Childhood Myopia. Ophthalmology, 113, 2285-2291.
http://dx.doi.org/10.1016/j.ophtha.2006.05.062

[18]   Chia, A., Chua, W.H., Cheung, Y.B., Wong, W.L., Lingham, A., Fong, A., et al. (2012) Atropine for the Treatment of Childhood Myopia: Safety and Efficacy of 0.5%, 0.1%, and 0.01% Doses (Atropine for the Treatment of Myopia 2). Ophthalmology, 119, 347-354.
http://dx.doi.org/10.1016/j.ophtha.2011.07.031

[19]   Yen, M.Y., Liu, J.H., Kao, S.C. and Shiao, C.H. (1989) Comparison of the Effect of Atropine and Cyclopentolate on Myopia. Annals of Ophthalmology, 21, 180-182, 187.

[20]   Fang, F., Huang, F., Xie, R., Li, C., Liu, Y., Zhu, Y., et al. (2015) Effects of Muscarinic Receptor Modulators on Ocular Biometry of Guinea Pigs. Ophthalmic and Physiological Optics, 35, 60-69.
http://dx.doi.org/10.1111/opo.12166

[21]   Zhou, X., Lu, F., Xie, R., Jiang, L., Wen, J., Li, Y., et al. (2007) Recovery from Axial Myopia Induced by a Monocularly Deprived Facemask in Adolescent (7-Week-Old) Guinea Pigs. Vision Research, 47, 1103-1111.
http://dx.doi.org/10.1016/j.visres.2007.01.002

[22]   Zhou, X., Qu, J., Xie, R., Wang, R., Jiang, L., Zhao, H., et al. (2006) Normal Development of Refractive State and Ocular Dimensions in Guinea Pigs. Vision Research, 46, 2815-2823.
http://dx.doi.org/10.1016/j.visres.2006.01.027

[23]   Palamar, M., Egrilmez, S., Uretmen, O., Yagci, A. and Kose, S. (2011) Influences of Cyclopentolate Hydrochloride on Anterior Segment Parameters with Pentacam in Children. Acta Ophthalmologica, 89, e461-e465.
http://dx.doi.org/10.1111/j.1755-3768.2011.02122.x

[24]   Le, Q.H., Cheng, N.N., Wu, W. and Chu, R.Y. (2005) Effect of Pirenzepine Ophthalmic Solution on Form-Deprivation Myopia in the Guinea Pigs. Chinese Medical Journal, 118, 561-566.

[25]   Ouyang, C.H., Chu, R.Y. and Hu, W.Z. (2003) Effects of Pirenzepine on Lens-Induced Myopia in the Guinea-Pig. Chinese Journal of Ophthalmology, 39, 348-351.

[26]   Mutti, D.O., Mitchell, G.L., Sinnott, L.T., Jones-Jordan, L.A., Moeschberger, M.L., Cotter, S.A., et al. (2012) Corneal and Crystalline Lens Dimensions before and after Myopia Onset. Optometry and Vision Science, 89, 251-262.

[27]   Gao, Q., Liu, Q., Ma, P., Zhong, X., Wu, J. and Ge, J. (2006) Effects of Direct Intravitreal Dopamine Injections on the Development of Lid-Suture Induced Myopia in Rabbits. Graefe’s Archive for Clinical and Experimental Ophthalmology, 244, 1329-1335.
http://dx.doi.org/10.1007/s00417-006-0254-1

[28]   McBrien, N.A., Stell, W.K. and Carr, B. (2013) How Does Atropine Exert Its Anti-Myopia Effects? Ophthalmic and Physiological Optics, 33, 373-378.
http://dx.doi.org/10.1111/opo.12052

[29]   Liu, Q., Wu, J., Wang, X. and Zeng, J. (2007) Changes in Muscarinic Acetylcholine Receptor Expression in Form Deprivation Myopia in Guinea Pigs. Molecular Vision, 13, 1234-1244.

[30]   Bitzer, M., Kovacs, B., Feldkaemper, M. and Schaeffel, F. (2006) Effects of Muscarinic Antagonists on ZENK Expression in the Chicken Retina. Experimental Eye Research, 82, 379-388.
http://dx.doi.org/10.1016/j.exer.2005.07.010

[31]   Coyle, J.T. and Snyder, S.H. (1969) Antiparkinsonian Drugs: Inhibition of Dopamine Uptake in the Corpus Striatum as a Possible Mechanism of Action. Science, 166, 899-901.
http://dx.doi.org/10.1126/science.166.3907.899

 
 
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