ABC  Vol.5 No.1 , February 2015
Development of Preparative Chromatography for Proteomic Approach of Mycorrhizal Symbiosis
Abstract: Although mechanism of symbiosis between arbuscular mycorrhizal fungi (AMF) and host plants has been investigated by genetic analysis, very little knowledge has been obtained because genome analysis of AMF is not perfect yet. Thus, we tried to develop mass purification of proteins using preparative chromatography in order to accelerate roteomic analysis of proteins related to mycorrhizal symbiosis, such as 24 and 53 kDa proteins. In particular, our data showed that 53 kDa proteins would be restrictively expressed when mycorrhizal fungi and host plants were stressed. However, 24 kDa proteins, which appear to be a usable indicator for the existence of various my-corrhizal fungi, were habitually detected in not only AMF but also other mycorrhizal fungi such as ectomycorrhizal fungi (EF). Moreover, we discovered new preparative chromatographical techniques for isolation and mass purification of those proteins. We are convinced that this chromato-graphical technique will greatly contribute to proteomic approach of mycorrhizal symbiosis.
Cite this paper: Matsubara, T. and Ishii, T. (2015) Development of Preparative Chromatography for Proteomic Approach of Mycorrhizal Symbiosis. Advances in Biological Chemistry, 5, 16-23. doi: 10.4236/abc.2015.51002.

[1]   Tisserant, E., et al. (2013) Genome of an Arbuscular Mycorrhizal Fungus Provides Insight into the Oldest Plant Symbiosis. Proceedings of the National Academy of Sciences of the United States of America, 110, 20117-20122.

[2]   Ishii, T., Matsubara, T., Yoneda, M. and Cruz, A.F. (2011) Arbuscular Mycorrhizal Fungi Increase Their Diversities by Taking Away Cell Nuclei from Plant Hosts. Horticulture Research (Japan), 10, 59.

[3]   Krüger, M., Kruger, C., Walker, C., Stockinger, H. and SchuBler, A. (2012) Phylogenetic Reference Data for Systematics and Phytotaxonomy of Arbuscular Mycorrhizal Fungi from Phylum to Species Level. New Phytologist, 193, 970-984.

[4]   Ishii, T., Ikeda, T., Rutto, K.L., Cruz, A.F., Matsumoto, I. and Kadoya, K. (1999) Proteins Related to the Mechanism of Symbiosis between Vesicular-Arbuscular Mycorrhizal Fungi and Plants. Journal of Japanese Society of Horticultural Science, 68, 229.

[5]   Matsubara, T., Yoneda, M. and Ishii, T. (2012) Fungal Isolate “KMI” Is a New Type of Orchid Mycorrhizal Fungus. American Journal of Plant Science, 3, 1121-1126.

[6]   Shannon, L.M., Kay, E. and Lew, J.Y. (1966) Peroxidase Isozymes from Horseradish Roots. 1. Isolation and Physical Properties. Journal of Biological Chemistry, 241, 166-2172.

[7]   Laemmli, U.K. (1970) Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4. Nature, 227, 680-685.

[8]   Bazghaleh, N., Hamel, C., Knight, J.D., Gan, Y., Vujanovic, V., Tar’an, B., Cruz, A.F. and Ishii, T. (2012) Phytochemical Regulation of Soil Health Chickpea’s Tool Box. Pulse Cluster Annual Meeting, Niagara Falls, 5-6 November 2012.

[9]   Wright, S.F. and Upadhyaya, A. (1996) Extraction of an Abundant and Unusual Protein from Soil and Comparison with Hyphal Protein of Arbuscular Mycorrhizal Fungi. Soil Science, 161, 575-586.

[10]   Gadkar, V. and Rillig, M.C. (2006) The Arbuscular Mycorrhizal Fungal Protein Glomalin Is a Putative Homolog of Heat Shock Protein 60. FEMS Microbiology Letters, 263, 93-101.

[11]   Unger, K.K., Janzen, R. and Jilge, G. (1987) Packings and Stationary Phases for Biopolymer Separations by HPLC. Chromatographia, 24, 144-154.

[12]   Couto, M.S.R., Paulo, E.L., Wipf, D. and Gaudot, E.D. (2013) Proteomic Studies of Arbuscular Mycorrhizal Associations. Advances in Biological Chemistry, 3, 48-58.

[13]   Ishii, T. and Horii, S. (2007) Culture of Mycorrhizal Fungi. Patent No. 4979551, Japan Patent Office.

[14]   Ishii, T. (2014) The Role and Use of Mycorrhizal Fungi. Rural Culture Association, Tokyo.