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 AJPS  Vol.6 No.1 , January 2015
Effect of the Ethanolic Extract of Khaya senegalensis on Some Biochemical Parameters on Rabbit’s in Glucose Overload Condition
Essé Agossou1, Mélanie Ahokpe1, Justin Behanzin1, Marcellin Takin1, Ganiou Yessoufou1, Loica Zohoun1, Lamine Baba-Moussa2, Alphonse Sezan1*
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Abstract: The effect of the ethanolic extract Khaya senegalensis on some biochemical parameters was tested in rabbits with normal glucose and glucose overload. Phytochemical screening after three batches of 6 rabbits race (TBD) were treated with the extract in oral doses of respectively 10, 25 and 50 mg/kg body weight for 30 days; three other groups receiving respectively these doses of the extract were placed under a priori temporary overload of glucose; a control group received instead distilled water treatment. Blood glucose, triglycerides and total cholesterol were measured by spectrophotometry and weight gain of the animals was performed at the beginning of the experiment and every 2 weeks during the study period. The phytochemical screening revealed the presence of polyphenolic compounds (majority) of saponins of anthracene derivatives and steroids in the extract. The test results indicate that under the dose of 50 mg/kg body weight (BW) there was a significant decrease (p < 0.05) in blood glucose after 24 hours. The extract had no effect on the lipid profile or body weight of rabbits.
Keywords: Rabbit, Khaya senegalensis, Glucose, Lipid Profile
Cite this paper: Agossou, E. , Ahokpe, M. , Behanzin, J. , Takin, M. , Yessoufou, G. , Zohoun, L. , Baba-Moussa, L. and Sezan, A. (2015) Effect of the Ethanolic Extract of Khaya senegalensis on Some Biochemical Parameters on Rabbit’s in Glucose Overload Condition. American Journal of Plant Sciences, 6, 240-248. doi: 10.4236/ajps.2015.61027.
References

[1]   Musa, H.R., Ambroggi, M., Souto, A. and Angeby, K.A. (2005) Drug Susceptibility Testing of Mycobacterium Tuberculosis by Nitrate Reductase Essay Applied Directly on Microscopy-Positive Sputum Sample. Journal of Clinical Microbiology, 43, 3159-3161. http://dx.doi.org/10.1128/JCM.43.7.3159-3161.2005

[2]   Sharma, B., Viswanath, G., Salunke, R. and Roy, P. (2008) Effects of Flavonoid-Rich Extract from Seeds of Eugenia jambolana (L.) on Carbohydrate and Lipid Metabolism in Diabetic Mice. Food Chemistry, 110, 697-705. http://dx.doi.org/10.1016/j.foodchem.2008.02.068

[3]   Guermaz, R., Zekri, S., Hatri, A., Kessal, F. and Brouri, M. (2008) Le diabète de type 2 en Algérie: Poids actuel et à venir. La Revue de Médecine Interne, 29, S49-S50.
http://dx.doi.org/10.1016/j.revmed.2008.03.087

[4]   Singh, J. and Kakkar, P. (2009) Antihyperglycemic and Antioxidant Effect of Berberis aristata Root Extract and Its Role in Regulating Carbohydrate Metabolism in Diabetic Lapins. Journal of Ethnopharmacology.

[5]   Zhou, I., Zhou, S., Tang, J., Zhang, K., Guang, L., Huang, Y., Xu, Y., Ying, Y., Zhang, L. and Li, D. (2009) Protective Effect of Berberine on Beta Cells in Streptozotocin- and High-Carbohydrate/High-Fat Diet-Induced Diabetic Lapins. European Journal of Pharmacology, 606, 262-268.
http://dx.doi.org/10.1016/j.ejphar.2008.12.056

[6]   Sarkhail, P., et al. (2007) Antidiabetic Effect of Phlomis anisodonta: Effects on Hepatic Cells Lipid Peroxidation and Antioxidant Enzymes in Experimental Diabetes. Pharmacological Research, 56, 261-266. http://dx.doi.org/10.1016/j.phrs.2007.07.003

[7]   Bahorun, T. (1997) Substances naturelles actives: La flore mauricienne, une source d’approvisionnement potentielle. Food and Agricultural Research, 83-94.

[8]   Gill, L.S. (1992) Ethnomedical Uses of Plants in Nigeria. Uniben Press, Benin City, 15-65.

[9]   Maydell, H.J. (1986) Trees and Shrubs of Sahel-Thier Charactérisatics and Uses. Gesdtschaft, Fur, Germany, 105-110.

[10]   Kolawole, S.O., et al. (2011) Effects of Aqueous Extract of Khaya senegalensis Stem Bark on Biochemical and Hematological Parameters in Rats. Journal of Pharmacological and Toxicological Methods, 6, 602-607.

[11]   Adebayo, J.O., Yacubu, M.T., Egwin, E.C., Owoyele, V.B. and Enaibe, B.U. (2003) Effect of Ethanolic Extract of Khaya senegalensis on Some Biochemical Parameters of Rat Kidney. Journal of Ethnopharmacology, 88, 69-72. http://dx.doi.org/10.1016/S0378-8741(03)00193-4

[12]   Lompo, M., Dubois, J. and Guissou, I.P. (2007) In Vitro Preliminary Study of Free Radical Scavenging Activity of Extracts from Khaya senegalensis A. Juss. Journal of Biological Sciences, 7, 677-680. http://dx.doi.org/10.3923/jbs.2007.677.680

[13]   Houghton, P.J. and Raman, A. (1998) Laboratory Handbook for Fractionation of Natural Extracts. Chapman and Hall, London, 199 p.

[14]   Allain, C.C., Poon, L.S., Chan, C.S., Richmond, W. and Fu, P.C. (1974) Enzymatic Determination of Total Serum Cholesterol. Clinical Chemistry, 20, 470-475.

[15]   Al-Achi, A. (2005) Herbs that Affect Blood Glucose Level. Women’s Health in Primary Care, 8, 325-330.

[16]   Pari, L. and Venkateswaran, S. (2002) Hypoglycaemic Activity of Scoparia dulcis L. Extract in Alloxan Induced Hyperglycaemic Rats. Phytotherapy Research, 16, 662-664.

[17]   Waltner-Law, M.E., Wang, X.L., Law, B.K., Hall, R.K., Nawano, M. and Granner, D.K. (2002) Epigallocatechin Gallate, a Constituent of Green Tea, Represses Hepatic Glucose Production. Journal of Biological Chemistry, 277, 34933- 34940. http://dx.doi.org/10.1074/jbc.M204672200

[18]   Vergès, B. (2007) Physiopathologie de la dyslipidémie du syndrome métabolique et du diabète de type 2. Nutrition Clinique et Métabolisme, 21, 9-16. http://dx.doi.org/10.1016/j.nupar.2007.01.011

 
 
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