Comparative Analysis of Salinity-Induced Proteomic Changes in Cotton (Gossypium hirsutum L.)
Author(s)
Yupeng Cui,
Xuke Lu,
Delong Wang,
Junjuan Wang,
Zujun Yin,
Weili Fan,
Shuai Wang,
Wuwei Ye*
Affiliation(s)
State Key Laboratory of Cotton Biology, Institute of Cotton Research of Chinese Academy of Agricultural Sciences, Anyang, China.
State Key Laboratory of Cotton Biology, Institute of Cotton Research of Chinese Academy of Agricultural Sciences, Anyang, China.
ABSTRACT
Salt stress on cotton varieties of distinct salinity tolerance can induce expression of different proteins. Zhong 07, a salt-tolerant variety and Zhong s9612, a salt-sensitive variety, were utilized as experimental materials. The leaves of trefoil seedlings treated with or without 0.4% NaCl for 24 h were harvested for whole-protein extraction. Two-dimensional technology, combined with mass spectroscopy (MS) analysis and protein database searching, was employed to detect differentially expressed proteins and determine their identities and biological functions. Compared with the control, Zhong 07 showed 10 differentially expressed proteins under salt stress, of which 6 were upregulated and 4 were downregulated. Meanwhile, 12 differentially expressed proteins were detected in Zhong s9612 under salt stress, of which 10 were upregulated and 2 were downregulated. In the matrix-assisted laser desorption-ionization/time of flight-time of flight/MS analysis, 14 differentially expressed proteins were successfully identified, including the ribulose-1, 5-bisphosphate carboxylase/oxygenase (RuBisco) large subunit-binding protein subunit alpha (RuBisco α), luminal binding protein (LBP), heat shock protein 70 (Hsp1, 2, 3), pathogenesis-related protein class 10 (PR-10), quinoneoxidoreductase-like protein (QOR), S-adenosylmethioninesyn-thetase (SAMS), enolase (EN), and RuBisco large subunit-binding protein subunit beta (RuBisco β). Cellular function is ultimately executed by proteins, and cotton varieties with different salt tolerance can be influenced by salt stress to various degrees, which can provide certain theoretical foundation for the identification of salt tolerance of cotton varieties. The findings also provide some proteins, such as the RuBisco large subunit binding proteins α and β subunits, OEE2 protein, HSP70, and S-adenosylmethionine synthetase, which can be used as protein markers of salt-to-lerance before- and post-treatment, making a big difference in salt-tolerance identification in cotton.
Salt stress on cotton varieties of distinct salinity tolerance can induce expression of different proteins. Zhong 07, a salt-tolerant variety and Zhong s9612, a salt-sensitive variety, were utilized as experimental materials. The leaves of trefoil seedlings treated with or without 0.4% NaCl for 24 h were harvested for whole-protein extraction. Two-dimensional technology, combined with mass spectroscopy (MS) analysis and protein database searching, was employed to detect differentially expressed proteins and determine their identities and biological functions. Compared with the control, Zhong 07 showed 10 differentially expressed proteins under salt stress, of which 6 were upregulated and 4 were downregulated. Meanwhile, 12 differentially expressed proteins were detected in Zhong s9612 under salt stress, of which 10 were upregulated and 2 were downregulated. In the matrix-assisted laser desorption-ionization/time of flight-time of flight/MS analysis, 14 differentially expressed proteins were successfully identified, including the ribulose-1, 5-bisphosphate carboxylase/oxygenase (RuBisco) large subunit-binding protein subunit alpha (RuBisco α), luminal binding protein (LBP), heat shock protein 70 (Hsp1, 2, 3), pathogenesis-related protein class 10 (PR-10), quinoneoxidoreductase-like protein (QOR), S-adenosylmethioninesyn-thetase (SAMS), enolase (EN), and RuBisco large subunit-binding protein subunit beta (RuBisco β). Cellular function is ultimately executed by proteins, and cotton varieties with different salt tolerance can be influenced by salt stress to various degrees, which can provide certain theoretical foundation for the identification of salt tolerance of cotton varieties. The findings also provide some proteins, such as the RuBisco large subunit binding proteins α and β subunits, OEE2 protein, HSP70, and S-adenosylmethionine synthetase, which can be used as protein markers of salt-to-lerance before- and post-treatment, making a big difference in salt-tolerance identification in cotton.
KEYWORDS
Upland Cotton (Gossypium hirsutum L.), Salt Stress, Differential Protein, Mass Spectrum Analysis
Upland Cotton (Gossypium hirsutum L.), Salt Stress, Differential Protein, Mass Spectrum Analysis
Cite this paper
Cui, Y. , Lu, X. , Wang, D. , Wang, J. , Yin, Z. , Fan, W. , Wang, S. and Ye, W. (2015) Comparative Analysis of Salinity-Induced Proteomic Changes in Cotton (Gossypium hirsutum L.). Agricultural Sciences, 6, 78-86. doi: 10.4236/as.2015.61007.
Cui, Y. , Lu, X. , Wang, D. , Wang, J. , Yin, Z. , Fan, W. , Wang, S. and Ye, W. (2015) Comparative Analysis of Salinity-Induced Proteomic Changes in Cotton (Gossypium hirsutum L.). Agricultural Sciences, 6, 78-86. doi: 10.4236/as.2015.61007.
References
[1] Parida, A.K. and Das, A.B. (2005) Salt Tolerance and Salinity Effects on Plants: A Review. Ecotoxicology and Environment Safety, 60, 324-349.http://www.ncbi.nlm.nih.gov/pubmed/15590011 [2] Ward, J.M., Hirschi, K.D. and Sze, H. (2003) Plants Pass the Salt. Trends Plant Science, 8, 200-201.
http://www.ncbi.nlm.nih.gov/pubmed/12758034 [3] Zhu, J.K. (2002) Salt and Drought Stress Signal Transduction in Plants. Annual Review Plant Biology, 53, 247-273. http://www.ncbi.nlm.nih.gov/pubmed/12221975
http://dx.doi.org/10.1146/annurev.arplant.53.091401.143329 [4] Ghosh, D., Tripathi, Y.K., Mujamdar, M.K., Kumar, R.V. and Prakash, V. (2006) Evaluation of Salt Tolerance Capability of Cotton (Gossypium hirsutum L.) Germplasm. Tropical Agriculture, 83, 30-36. [5] Meloni, D.A., Oliva, M.A., Martinez, C.A. and Cambraia, J. (2003) Photosynthesis and Activity of Superoxide Dismutase, Peroxidase and Glutathione Reductase in Cotton under Salt Stress. Environmental and Experimental Botany, 49, 69-76.
http://dx.doi.org/10.1016/S0098-8472(02)00058-8 [6] Li, X.X., Liu, B.X., Guo, Z.T., Chang, Y.X., He, L., et al. (2013) Effects of NaCl Stress on Photosynthesis Characteristics and Fast Chlorophyll Fluorescence Induction Dynamics of Pistaciachinensis Leaves. Ying Yong Sheng Tai Xue Bao, 24, 2479-2484. http://www.ncbi.nlm.nih.gov/pubmed/24417104 [7] Ashraf, M. (2002) Salt Tolerance of Cotton: Some New Advances. Critical Reviews in Plant Sciences, 21, 1-30. http://www.ingentaconnect.com/content/tandf/bpts/2002/00000021/00000001/art00001
http://dx.doi.org/10.1016/S0735-2689(02)80036-3 [8] Tripathi, A.K. and Tripathi, S. (1999) Changes in Same Physiological and Biochemical Characters in Albizialebbek as Bio-Indicators of Heavy Metal Toxicity. Journal of Environmental Biology, 20, 93-98. [9] Sun, C.H., Du, W., Cheng, X.L., Xu, X.N., Zhang, Y.H., et al. (2010) The Effects of Drought Stress on the Activity of Acid Phosphatase and Its Protective Enzymes in Pigweed Leaves. African Journal of Biotechnology, 9, 825-833. [10] Furtado, R.F., Mano, A.R.D., Alves, C.R., de Freitas, S.M. and Filho, S.M. (2007) Effect of Salinity on the Germination of Cotton Seeds. Revista Ciencia Agronomica, 38, 224-227. [11] Meneses, C.H.S.G., Bruno, R.D.A., Fernandes, P.D., Pereira, W.E., de Morais Lima, L.H.G., de Andrade Lima, M.M. and Vidal, M.S. (2011) Germination of Cotton Cultivar Seeds under Water Stress Induced by Polyethyleneglycol-6000. Scientia Agricola, 68, 131-138.
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162011000200001&lng=en&nrm=
iso&tlng=en http://dx.doi.org/10.1590/S0103-90162011000200001 [12] Smith, L.H., Keys, A.J. and Michael, C.W.E. (1995) Striga hermonthica Decreases Photosynthesis in Zea mays through Effects on Leaf Cell Structure. Journal of Experimental Botany, 46, 759-765.
http://dx.doi.org/10.1093/jxb/46.7.759 [13] Yu, L.N. and Pei, J. (2011) Application of Differential Proteomics in Mechanism Research of Acupuncture. Zhong Xi Yi Jie He Xue Bao, 9, 819-823.
http://www.ncbi.nlm.nih.gov/pubmed/21849141 [14] Zhang, G.W., Lu, H.L., Zhang, L., Chen, B.L. and Zhou, Z.G. (2011) Salt Tolerance Evaluation of Cotton (Gossypium hirsutum) at Its Germinating and Seedling Stages and Selection of Related Indices. Ying Yong Sheng Tai Xue Bao, 22, 2045-2053.
http://www.ncbi.nlm.nih.gov/pubmed/22097366 [15] Mechin, V., Damerval, C. and Zivy, M. (2007) Total Protein Extraction with TCA-Acetone. Methods in Molecular Biology, 355, 1-8. http://www.ncbi.nlm.nih.gov/pubmed/17093296 [16] Wu, X., Gong, F. and Wang, W. (2014) Protein Extraction from Plant Tissues for 2DE and Its Application in Proteomic Analysis. Proteomics, 14, 645-658.
http://www.ncbi.nlm.nih.gov/pubmed/24395710
http://dx.doi.org/10.1002/pmic.201300239 [17] Kottapalli, K.R., Payton, P., Rakwal, R., Agrawal, G.K., Shibato, J., Burow, M. and Puppala, N. (2008) Proteomics Analysis of Mature Seed of Four Peanut Cultivars Using Two-Dimensional Gel Electrophoresis Reveals Distinct Differential Expression of Storage, Anti-Nutritional, and Allergenic Proteins. Plant Science, 175, 321-329. http://dx.doi.org/10.1016/j.plantsci.2008.05.005 [18] Law, R.D., Crafts-Brandner, S.J. and Salvucci, M.E. (2001) Heat Stress Induces the Synthesis of a New Form of Ribulose-1, 5-bisphosphate Carboxylase/Oxygenase Activase in Cotton Leaves. Planta, 214, 117-125. http://www.ncbi.nlm.nih.gov/pubmed/11762161
http://dx.doi.org/10.1007/s004250100592 [19] Spreitzer, R.J. (2003) Role of the Small Subunit in Ribulose-1,5-bisphosphate Carboxylase/Oxygenase. Archives of Biochemistry and Biophysics, 414, 141-149.
http://www.ncbi.nlm.nih.gov/pubmed/12781765
http://dx.doi.org/10.1016/S0003-9861(03)00171-1 [20] Chaves, M.M., Flexas, J. and Pinheiro, C. (2009) Photosynthesis under Drought and Salt Stress: Regulation Mechanisms from Whole Plant to Cell. Annals of Botany, 103, 551-560. http://www.ncbi.nlm.nih.gov/pubmed/18662937
http://dx.doi.org/10.1093/aob/mcn125 [21] Van der Straeten, D., Rodrigues-Pousada, R.A., Goodman, H.M. and Van Montagu, M. (1991) Plant Enolase: Gene Structure, Expression, and Evolution. Plant Cell, 3, 719-735.
http://www.ncbi.nlm.nih.gov/pubmed/1841726
http://dx.doi.org/10.1105/tpc.3.7.719 [22] Hua, S.B., Dube, S.K., Barnett, N.M. and Kung, S.D. (1991) Nucleotide Sequence of Gene oee2-A and Its cDNA Encoding 23 kDa Polypeptide of the Oxygen-Evolving Complex of Photosystem II in Tobacco. Plant Molecular Biology, 17, 551-553. http://www.ncbi.nlm.nih.gov/pubmed/1884009
http://dx.doi.org/10.1007/BF00040655 [23] Hua, S.B., Dube, S.K. and Kung, S.D. (1995) Organ-Specific Expression of the Nuclear Gene Encoding OEE2 of Photosystem-II Oxygen-Evolving Complex in Nicotiana tabacum. Journal of Plant Biochemistry and Biotechnology, 4, 109-111. http://dx.doi.org/10.1007/BF03262964 [24] Hurley, J.H. (1996) The Sugar Kinase Heat Shock Protein 70 Actin Super Family: Implications of Conserved Structure for Mechanism. Annual Review of Biophysics and Biomolecular Structure, 25, 137-162. http://www.ncbi.nlm.nih.gov/pubmed/8800467 [25] Park, C.J., Kim, K.J., Shin, R., Park, J.M., Shin, Y.C. and Paek, K.H. (2004) Pathogenesis-Related Protein 10 Isolated from Hot Pepper Functions as a Ribonuclease in an Antiviral Pathway. Plant Journal, 37, 186-198. http://www.ncbi.nlm.nih.gov/pubmed/14690503
http://dx.doi.org/10.1046/j.1365-313X.2003.01951.x
[1] Parida, A.K. and Das, A.B. (2005) Salt Tolerance and Salinity Effects on Plants: A Review. Ecotoxicology and Environment Safety, 60, 324-349.http://www.ncbi.nlm.nih.gov/pubmed/15590011 [2] Ward, J.M., Hirschi, K.D. and Sze, H. (2003) Plants Pass the Salt. Trends Plant Science, 8, 200-201.
http://www.ncbi.nlm.nih.gov/pubmed/12758034 [3] Zhu, J.K. (2002) Salt and Drought Stress Signal Transduction in Plants. Annual Review Plant Biology, 53, 247-273. http://www.ncbi.nlm.nih.gov/pubmed/12221975
http://dx.doi.org/10.1146/annurev.arplant.53.091401.143329 [4] Ghosh, D., Tripathi, Y.K., Mujamdar, M.K., Kumar, R.V. and Prakash, V. (2006) Evaluation of Salt Tolerance Capability of Cotton (Gossypium hirsutum L.) Germplasm. Tropical Agriculture, 83, 30-36. [5] Meloni, D.A., Oliva, M.A., Martinez, C.A. and Cambraia, J. (2003) Photosynthesis and Activity of Superoxide Dismutase, Peroxidase and Glutathione Reductase in Cotton under Salt Stress. Environmental and Experimental Botany, 49, 69-76.
http://dx.doi.org/10.1016/S0098-8472(02)00058-8 [6] Li, X.X., Liu, B.X., Guo, Z.T., Chang, Y.X., He, L., et al. (2013) Effects of NaCl Stress on Photosynthesis Characteristics and Fast Chlorophyll Fluorescence Induction Dynamics of Pistaciachinensis Leaves. Ying Yong Sheng Tai Xue Bao, 24, 2479-2484. http://www.ncbi.nlm.nih.gov/pubmed/24417104 [7] Ashraf, M. (2002) Salt Tolerance of Cotton: Some New Advances. Critical Reviews in Plant Sciences, 21, 1-30. http://www.ingentaconnect.com/content/tandf/bpts/2002/00000021/00000001/art00001
http://dx.doi.org/10.1016/S0735-2689(02)80036-3 [8] Tripathi, A.K. and Tripathi, S. (1999) Changes in Same Physiological and Biochemical Characters in Albizialebbek as Bio-Indicators of Heavy Metal Toxicity. Journal of Environmental Biology, 20, 93-98. [9] Sun, C.H., Du, W., Cheng, X.L., Xu, X.N., Zhang, Y.H., et al. (2010) The Effects of Drought Stress on the Activity of Acid Phosphatase and Its Protective Enzymes in Pigweed Leaves. African Journal of Biotechnology, 9, 825-833. [10] Furtado, R.F., Mano, A.R.D., Alves, C.R., de Freitas, S.M. and Filho, S.M. (2007) Effect of Salinity on the Germination of Cotton Seeds. Revista Ciencia Agronomica, 38, 224-227. [11] Meneses, C.H.S.G., Bruno, R.D.A., Fernandes, P.D., Pereira, W.E., de Morais Lima, L.H.G., de Andrade Lima, M.M. and Vidal, M.S. (2011) Germination of Cotton Cultivar Seeds under Water Stress Induced by Polyethyleneglycol-6000. Scientia Agricola, 68, 131-138.
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162011000200001&lng=en&nrm=
iso&tlng=en http://dx.doi.org/10.1590/S0103-90162011000200001 [12] Smith, L.H., Keys, A.J. and Michael, C.W.E. (1995) Striga hermonthica Decreases Photosynthesis in Zea mays through Effects on Leaf Cell Structure. Journal of Experimental Botany, 46, 759-765.
http://dx.doi.org/10.1093/jxb/46.7.759 [13] Yu, L.N. and Pei, J. (2011) Application of Differential Proteomics in Mechanism Research of Acupuncture. Zhong Xi Yi Jie He Xue Bao, 9, 819-823.
http://www.ncbi.nlm.nih.gov/pubmed/21849141 [14] Zhang, G.W., Lu, H.L., Zhang, L., Chen, B.L. and Zhou, Z.G. (2011) Salt Tolerance Evaluation of Cotton (Gossypium hirsutum) at Its Germinating and Seedling Stages and Selection of Related Indices. Ying Yong Sheng Tai Xue Bao, 22, 2045-2053.
http://www.ncbi.nlm.nih.gov/pubmed/22097366 [15] Mechin, V., Damerval, C. and Zivy, M. (2007) Total Protein Extraction with TCA-Acetone. Methods in Molecular Biology, 355, 1-8. http://www.ncbi.nlm.nih.gov/pubmed/17093296 [16] Wu, X., Gong, F. and Wang, W. (2014) Protein Extraction from Plant Tissues for 2DE and Its Application in Proteomic Analysis. Proteomics, 14, 645-658.
http://www.ncbi.nlm.nih.gov/pubmed/24395710
http://dx.doi.org/10.1002/pmic.201300239 [17] Kottapalli, K.R., Payton, P., Rakwal, R., Agrawal, G.K., Shibato, J., Burow, M. and Puppala, N. (2008) Proteomics Analysis of Mature Seed of Four Peanut Cultivars Using Two-Dimensional Gel Electrophoresis Reveals Distinct Differential Expression of Storage, Anti-Nutritional, and Allergenic Proteins. Plant Science, 175, 321-329. http://dx.doi.org/10.1016/j.plantsci.2008.05.005 [18] Law, R.D., Crafts-Brandner, S.J. and Salvucci, M.E. (2001) Heat Stress Induces the Synthesis of a New Form of Ribulose-1, 5-bisphosphate Carboxylase/Oxygenase Activase in Cotton Leaves. Planta, 214, 117-125. http://www.ncbi.nlm.nih.gov/pubmed/11762161
http://dx.doi.org/10.1007/s004250100592 [19] Spreitzer, R.J. (2003) Role of the Small Subunit in Ribulose-1,5-bisphosphate Carboxylase/Oxygenase. Archives of Biochemistry and Biophysics, 414, 141-149.
http://www.ncbi.nlm.nih.gov/pubmed/12781765
http://dx.doi.org/10.1016/S0003-9861(03)00171-1 [20] Chaves, M.M., Flexas, J. and Pinheiro, C. (2009) Photosynthesis under Drought and Salt Stress: Regulation Mechanisms from Whole Plant to Cell. Annals of Botany, 103, 551-560. http://www.ncbi.nlm.nih.gov/pubmed/18662937
http://dx.doi.org/10.1093/aob/mcn125 [21] Van der Straeten, D., Rodrigues-Pousada, R.A., Goodman, H.M. and Van Montagu, M. (1991) Plant Enolase: Gene Structure, Expression, and Evolution. Plant Cell, 3, 719-735.
http://www.ncbi.nlm.nih.gov/pubmed/1841726
http://dx.doi.org/10.1105/tpc.3.7.719 [22] Hua, S.B., Dube, S.K., Barnett, N.M. and Kung, S.D. (1991) Nucleotide Sequence of Gene oee2-A and Its cDNA Encoding 23 kDa Polypeptide of the Oxygen-Evolving Complex of Photosystem II in Tobacco. Plant Molecular Biology, 17, 551-553. http://www.ncbi.nlm.nih.gov/pubmed/1884009
http://dx.doi.org/10.1007/BF00040655 [23] Hua, S.B., Dube, S.K. and Kung, S.D. (1995) Organ-Specific Expression of the Nuclear Gene Encoding OEE2 of Photosystem-II Oxygen-Evolving Complex in Nicotiana tabacum. Journal of Plant Biochemistry and Biotechnology, 4, 109-111. http://dx.doi.org/10.1007/BF03262964 [24] Hurley, J.H. (1996) The Sugar Kinase Heat Shock Protein 70 Actin Super Family: Implications of Conserved Structure for Mechanism. Annual Review of Biophysics and Biomolecular Structure, 25, 137-162. http://www.ncbi.nlm.nih.gov/pubmed/8800467 [25] Park, C.J., Kim, K.J., Shin, R., Park, J.M., Shin, Y.C. and Paek, K.H. (2004) Pathogenesis-Related Protein 10 Isolated from Hot Pepper Functions as a Ribonuclease in an Antiviral Pathway. Plant Journal, 37, 186-198. http://www.ncbi.nlm.nih.gov/pubmed/14690503
http://dx.doi.org/10.1046/j.1365-313X.2003.01951.x