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 AJPS  Vol.6 No.1 , January 2015
Antioxidant Activities of Ethanoilc Extracts of Spilanthes uliginosa, Ocimum basilicum, Hyptis spicigera and Cymbopogon citratus against Swiss Mice Exposed to Plasmodium berghei Anka 65
Abstract: Malaria infection is associated with increased generation of free radicals. This study investigated the antioxidant activity of ethanolic leaf extracts of Spilanthes uliginosa, Ocimum basilicum, Hyptis spicigera and Cymbopogon citratus. Seventy two (72) swiss mice of both sexes were used. All the mice were treated intraperitoneally with 0.2 ml parasitized blood suspension and parasitemia assessed by thin blood films stained with Geimsa stain after seventy two hours. The mice were divided into six groups namely; A, B, C, D, E, and F of twelve mice each. Groups B, C, D and E were subdivided into three (3): B1, B2, B3, C1, C2, C3, D1, D2, D3, E1, E2 and E3, four in each subgroup. The subgroups were treated with the extracts of Spilanthes uliginosa (Sw), Ocimum basilicum, Hyptis spiligera and Cymbopogon citratus each for five (5) consecutive days with 200, 400 and 800 mg/kg body weight respectively via oral intubation. Two control groups, A and F were used. The negative control (A) was treated daily with 5 ml/kg normal saline while positive control group (F) was treated with 5 mg/kg body weight of chloroquine. The results indicated a general significant (P < 0.05) decrease in the lipid peroxidation concentrations of the parasitized treated mice when compared to parasitized untreated mice on the last day. A general significant dose dependent increase (P < 0.05) was observed in superoxide dismutase (SOD), catalase and glutathione perioxidase activities as well as reduced glutathione concentrations in the treated mice except at a dosage of 200 mg/kg body weight for all the plants. The effects of the extracts were significantly higher (P < 0.05) than that of chloroquine. These results suggest that the ethanolic extracts of these plants may contribute to the protection of malaria infected mice against oxidative damage by improving antioxidant status in a dose dependent manner.
Cite this paper: Agbafor, K. , Uraku, A. , Okaka, A. , Ibiam, U. , Ajah, P. and Obasi, O. (2015) Antioxidant Activities of Ethanoilc Extracts of Spilanthes uliginosa, Ocimum basilicum, Hyptis spicigera and Cymbopogon citratus against Swiss Mice Exposed to Plasmodium berghei Anka 65. American Journal of Plant Sciences, 6, 64-72. doi: 10.4236/ajps.2015.61008.
References

[1]   Maciel, M.A.M., Pinto, A.C., Veiga Jr., V.F., Grynberg, N.F. and Echevarria, A. (2002) Medicinal Plants: The Need for Multidisciplinary Scientific Studies. Quim Nova, 25, 429-438.
http://dx.doi.org/10.1590/S0100-40422002000300016

[2]   Agbafor, K.N. and Akubugwo, E.I. (2007) Hypocholesterolemic Effect of Ethanol Extract of Fresh Leaves of Cymbopogon citratus. African Journal of Biotechnology, 6, 596-598.

[3]   Oyedemi, S.O., Bradley, G. and Afolayan, A.J. (2010) In-Vitro and -Vivo Antioxidant Activities of Aqueous Extract of Strychnos henningsii Gilg. African Journal of Pharmacy and Pharmacology, 4, 70-78.

[4]   WHO (2011) World Malaria Report. World Health Organization Press, Geneva.

[5]   Tripathi, K.D. (2013) Essentials of Medical Pharmacology. 7th Edition, Jaypee Brothers Medical Publishers (P) Ltd., New Delhi, 816-835.

[6]   Sandro, P., Danilo, R.M., Bruno, A.Q.G., Michelli, E.S.F., Ana, C.M.G., Paula, S.O.C.L., Thyago, C.V., Maria, F.D. and Michael, D.G. (2012) Oxidative Stress in Malaria. International Journal of Molecular Sciences, 13, 16346-16372. http://dx.doi.org/10.3390/ijms131216346

[7]   Potter, S.M., Mitchell, A.J., Cowden, W.B., Sanni, A., Dinaner, M., Hann, J.B. and Hunt, N.H. (2005) Phagocyte-De-Rived Reactive Oxygen Species Do Not Influence the Progreeion of Murine Blood-Stage Malaria Infection. Infection Immunology, 73, 4941-4947.
http://dx.doi.org/10.1128/IAI.73.8.4941-4947.2005

[8]   Keller, C.C., Kremesner, P.G., Hittner, J.B., misukonis, M.A., Weinberg, J.B. and Perkins, D.J. (2004) Elevated Nitric Oxide Production in Children with Malarial Anemia: Hemozoin-Induced Nitric Oxide Synthase Type 2 Transcripts and Nitric Oxide in Blood Mononuclear Cells. Infection Immunology, 72, 4868-4873. http://dx.doi.org/10.1128/IAI.72.8.4868-4873.2004

[9]   Sohail, M., Kaul, A., Raziuddin, M. and Adak, T. (2007) Decreased Glutathione-S-Transferase Activity Diagnostic and Protective Role in Vivax Malaria. Clinical Biochemistry, 40, 377-382.
http://dx.doi.org/10.1016/j.clinbiochem.2007.01.005

[10]   Guha, M., Kumar, S., Choubey, V., Maity, P. and Bandyopadhya, U. (2006) Apoptosis in Liver during Malaria: Role of Oxidative Stress and Implication of Mitochondrial Pathway. FASEB Journal, 20, 439-449. http://dx.doi.org/10.1096/fj.05-5338fje

[11]   Doekrell, H.M. and Playfair, J.H. (1984) Killing of Plasmodium yoelii by Enzyme-Induced Products of the Oxidative Burst. Infection and Immunity, 43, 451-456.

[12]   Erel, O., Kocyigit, A., Avci, S., Aktepe, N. and Bulut, V. (1997) Oxidative Stress and Antioxidative Status of Plasma and Erythrocytes in Patients with Vivax Malaria. Clinical Biochemistry, 30, 631-639.
http://dx.doi.org/10.1016/S0009-9120(97)00119-7

[13]   Yakubu, M.T. and Omoniwa, B.P. (2012) Effects of Aqueous Stem Extract of Massularia Acuminata on Some Liver Function Indices of Male Rats. Iranian Journal of Toxicology, 6, 716-722.

[14]   Umoh, I., Oyebadejo, S., Bassey, E. and Nnah, U. (2013) Chronic Consumption of Combined Extracts of Abelmoschus esculentus and Piper guineense Induced Hepatoxicity in Wistar Rats: Histopathological Study. International Journal of Pharmaceutical and Biomedical Science, 4, 73-77.

[15]   Moron, M.S., Depierre, J.W. and Mannervik, B. (1979) Levels of Glutathione, Glutathione Reductase and Glutathione S-Transferase Activities in Rat Lung and Liver. Biochimica et Biophysica Acta, 582, 67-78. http://dx.doi.org/10.1016/0304-4165(79)90289-7

[16]   Niehius, W.G. and Samuelson, B. (1968) Formation of Malonaldehyde from Phospholipid Arachidonate during Microsomal Lipid Peroxidation. European Journal of Biochemistry, 6, 126-130.
http://dx.doi.org/10.1111/j.1432-1033.1968.tb00428.x

[17]   Akanbi, O.M., Odaibo, A.B. and Ademowo, O.G. (2010) Effects of Antimalarial Drugs and Malaria Infection on Oxidative Stress in Pregnant Women. African Journal of Reproductive Health, 14, 209-212.

[18]   George, B.O., Osioma, E., Okpoghono, J. and Aina, O.O. (2011) Changes in Liver and Serum Transaminases and Alkaline Phosphatase Enzyme Activities in Plasmodium berghei Infected Mice Treated with Aqueous Extract of Aframomum sceptrum. African Journal of Biochemistry Research, 5, 277-281.

[19]   Romero, R., Chaiworapongsa, T. and Espinoza, J. (2003) Micronutrients and Intrauterine Infection, Premature Birth and the Fetal Inflammatory Response Syndrome. Journal of Nutrition, 133, 1668S-1673S.

[20]   Khuwaja, G., Khan, M.M., Ishrat, T., Ahmad, A., Raza, S.S., Ashafaq, M., Javed, H., Khan, M.B., Khan, A., Vaibhav, K., Safhi, M.M. and Islam, F. (2010) Neuroprotective Effects of Curcumin on 6-hydroxydopamine-Induced Parkinsonism in Rats: Behavioral, Neurochemical and Immunohistochemical Studies. Brain Research, 1368, 254-263.

[21]   Demicheli, V., Quijano, C., Alvarez, B. and Radi, R. (2007) Inactivation and Nitration of Human Superoxide Dismutase (SOD) by Fluxes of Nitric Oxide and Superoxide. Free Radical Biology and Medicine, 42, 1359-1368. http://dx.doi.org/10.1016/j.freeradbiomed.2007.01.034

[22]   Murray, R.K., Granner, D.K. and Rodwell, V.W. (2006) Harper’s Illustrated Biochemistry. 27th Edition, McGraw Hill Education, New York, 400-500.

 
 
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