Gas Exchanges and Biological Nitrogen Fixation in Soybean under Water Restriction

Paula  Cerezini; Marco Antonio  Nogueira; Mariangela  Hungria; Antonio Eduardo  Pípolo

doi:10.4236/ajps.2014.526419

Paula Cerezini¹, Antonio Eduardo Pípolo², Mariangela Hungria², Marco Antonio Nogueira²

Abstract: Biological nitrogen fixation (BNF) in soybean is vulnerable to drought; however, there are genotypic variations among soybean cultivars regarding the ability to keep BNF under moderate water restriction. The aim of this study was to evaluate parameters related to gas exchanges and regulation of BNF in soybean genotypes BNF drought-tolerant (R01-581F and R01-416F), or drought-susceptible (CD 215 and BRS 317), submitted to adequate water supply or restriction between 45 and 55 days after emergence. We searched for traits associated with tolerance/susceptibility to drought, which might be useful in the selection of drought-tolerant soybean genotypes. Plant biomass was not affected under water restriction, but the number and dry weight of nodules reduced by 33% and 12%, respectively, in the average of genotypes. Drought-tolerant genotypes were more effective in maintaining gas exchanges under water restriction. Under water restriction, all genotypes increased the concentration of ureides in nodules, but only the susceptible genotypes showed that in leaves. The maintenance of gas exchanges and N metabolism regulation under water restriction in genotype R01-581F suggests that these parameters may be used to characterize soybean genotypes that can be sources of drought tolerance in genetic breeding programs.

Keywords: Bradyrhizobium, Drought, Glycine max, Photosynthesis, Ureides

Cite this paper: Cerezini, P. , Pípolo, A. , Hungria, M. and Nogueira, M. (2014) Gas Exchanges and Biological Nitrogen Fixation in Soybean under Water Restriction. American Journal of Plant Sciences, 5, 4011-4017. doi: 10.4236/ajps.2014.526419.

References

[1] CONAB (COMPANHIA NACIONAL De ABASTECIMENTO) (2014) Acompanhamento da safra brasileira: Grãos, décimo levantamento, CONAB, safra 2013/2014. http://www.conab.gov.br

[2] Hungria, M., Campo, R.J. and Mendes, I.C. (2007) A importancia do processo de fixação biológica do nitrogênio para a cultura da soja: Componente essencial para a competitividade do produto brasileiro. Embrapa Soja. Documentos, 283, Embrapa Soja, Londrina.
http://www.infoteca.cnptia.embrapa.br/handle/doc/468512

[3] Sinclair, T.R. and Vadez, V. (2012) The Future of Grain Legumes in Cropping Systems. Crop Pasture Science, 63, 501-512. http://dx.doi.org/10.1071/CP12128

[4] IPCC (Intergovernmental Panel on Climate Change) (2007) Climate Change: The Physical Science Basis. Contribution of Working Group I to the Fourth Assessment Report of the Intergovernmental Panel on Climate Change. IPCC, Paris. http://www.ipcc.ch

[5] Sinclair, T.R., Purcell, L.C., King, A., Sneller, C.H., Chen, P. and Vadez, V. (2007) Drought Tolerance and Yield Increase of Soybean Resulting from Improved Symbiotic N2 Fixation. Field Crops Research, 101, 68-71. http://dx.doi.org/10.1016/j.fcr.2006.09.010

[6] Serraj, R., Sinclair, T.R. and Purcell, L.C. (1999) Symbiotic N2 Fixation Response to Drought. Journal of Experimental Botany, 50, 143-155.

[7] Ladreira, R., Marino, D., Larrainzar, E., González, E.M. and Arrese-Igor, C. (2007) Reduced Carbon Availability to Bacteroids and Elevated Ureides in Nodules, but Not in Shoots, Are Involved in the Nitrogen Fixation Response to Early Drought in Soybean. Plant Physiology, 145, 539-546.
http://dx.doi.org/10.1104/pp.107.102491

[8] Chen, P., Sneller, C.H., Purcell, L.C., Sinclair, T.R., King, C.A. and Ishibashi, T. (2007) Registration of Soybean Germplasm Lines R01-416F and R01-581F for Improved Yield and Nitrogen Fixation under Drought Stress. Journal of Plant Registrations, 1, 166-167.
http://dx.doi.org/10.3198/jpr2007.01.0046crg

[9] EMBRAPA (Empresa Brasileira de Pesquisa Agropecuária) (1997) Manual de métodos de análise de solo. EMBRAPA-CNPS. Documentos 1, Centro Nacional de Pesquisa de Solos, EMBRAPA, Rio de Janeiro.

[10] Kaschuk, G., Hungria, M., Leffelaar P.A., Giller, K.E. and Kuyper, T.W. (2010) Differences in Photosynthetic Behaviour and Leaf Senescence of Soybean [Glycine max (L.) Merrill] Dependent on N2 Fixation or Nitrate Supply. Plant Biology, 12, 60-69.
http://dx.doi.org/10.1111/j.1438-8677.2009.00211.x

[11] Searle, P.L. (1984) The Berthelot or Indophenol Reaction and Its Use in the Analytical Chemistry of Nitrogen. Analyst, 109, 549-568. http://dx.doi.org/10.1039/an9840900549

[12] Vogels, G.D. and van der Drift, C. (1970) Differential Analysis of Glyoxylate Derivatives. Analytical Biochemistry, 33, 143-157. http://dx.doi.org/10.1016/0003-2697(70)90448-3

[13] Hungria, M. and Kaschuk, G. (2014) Regulation of N2 Fixation and NO3^-/NH4+ Assimilation in Nodulated and N-Fertilized Phaseolus vulgaris L. Exposed to High Temperature Stress. Environmental and Experimental Botany, 98, 32-39.
http://dx.doi.org/10.1016/j.envexpbot.2013.10.010

[14] Silvente, S., Sobolev, A.P. and Lara, M. (2012) Metabolite Adjustments in Drought Tolerant and Sensitive Soybean Genotypes in Response to Water Stress. PLoS ONE, 7, e38554.

[15] Lopes, M.S., Araus, J.L., van Heerden, P.D.R. and Foyer, C.H. (2011) Enhancing Drought Tolerance in C4 Crops. Journal of Experimental Botany, 62, 3135-3153.