AJPS  Vol.5 No.25 , December 2014
The Effect of Fusarium Inoculation and Fungicide Application on Concentrations of Flavonoids (Apigenin, Kaempferol, Luteolin, Naringenin, Quercetin, Rutin, Vitexin) in Winter Wheat Cultivars
Abstract: Grain of 30 winter wheat cultivars was screened for the content of seven flavonoids (apigenin, kaempferol, luteolin, naringenin, quercetin, rutin, vitexin). In total, 90 samples were analyzed, belonging to three combinations: the control, samples inoculated with Fusarium culmorum, and samples inoculated with Fusarium culmorum and protected with fungicides (Prosaro, Topsin M). Analyzed flavonoids were detected in all tested samples. The highest concentrations were recorded for quercetin (512.9 mg/kg) and luteolin (458.4 mg/kg). Concentration of all compounds was the highest in the inoculated samples, while in the control and in the inoculated samples treated with fungicides their contents were significantly lower and similar, as confirmed by the statistical analysis conducted by multivariate methods. In most cases, significant correlations were obtained between individual flavonoids. Such correlations were observed for all flavonoids in samples covering all the three variants and almost all flavonoids in the inoculated samples. The lowest number of correlations was recorded for samples treated with fungicides.
Cite this paper: Buśko, M. , Góral, T. , Ostrowska, A. , Matysiak, A. , Walentyn-Góral, D. and Perkowski, J. (2014) The Effect of Fusarium Inoculation and Fungicide Application on Concentrations of Flavonoids (Apigenin, Kaempferol, Luteolin, Naringenin, Quercetin, Rutin, Vitexin) in Winter Wheat Cultivars. American Journal of Plant Sciences, 5, 3727-3736. doi: 10.4236/ajps.2014.525389.

[1]   Pichersky, E. and Gang, D.R. (2000) Genetics and Biochemistry of Secondary Metabolites in Plants: An Evolutionary Perspective. Trends in Plant Science Perspectives, 5, 439-445.

[2]   Dykes, L. and Rooney, W. (2007) Phenolic Compounds in Cereal Grains and Their Health Benefits. Cereal Foods World, 52, 105-111.

[3]   Ray Narayana, K., Sripal Reddy, M., Chaluvadi, M.R. and Krishna, D.R. (2001) Bioflavonoids Classification, Pharmacological, Biochemical Effects and Therapeutic Potential. Indian Journal of Pharmacology, 33, 2-16.

[4]   Xu, Y.C., Leung, S.W.S, Yeung, D.K.Y., Hu, L.H., Chen, G.H., Che, C.M. and Man, R.Y.K. (2007) Structure Activity Relationship of Flavonoids for Vascular Relaxation in Porcine Coronary Artery. Phytochemistry, 68, 1179-1188.

[5]   Erlund, I. (2004) Review of the Flavonoids Quercetin, Hesperetin, and Naringenin. Dietary Sources, Bioactivities, Bioavalability. Nutrition Research, 24, 851-874.

[6]   Naczk, M. and Shahidi, F. (2006) Phenolics in Cereals, Fruits and Vegetables: Occurrence, Extraction and Analysis. Journal of Pharmaceutical and Biomedical Analysis, 41, 1523-1542.

[7]   Abdel-Aal, E.-S.M. and Hucl, P. (2003) Composition and Stability of Anthocyanins in Blue-Grained Wheat. Journal of Agriculture and Food Chemistry, 51, 2174-2180.

[8]   Abdel-Aal, E.-S.M., Young, J.C. and Rabalski, I. (2006) Anthocyanin Composition in Black, Blue, Pink, and Red Cereal Grains. Journal of Agriculture and Food Chemistry, 54, 4696-4704.

[9]   Shahidi, F. and Naczk, M. (1995) Food Phenolics: Sources, Chemistry, Effects, Applications. Technomic Publishing, Lancaster.

[10]   Wojakowska, A., Perkowski, J., Góral, T. and Stobiecki, M. (2012) Structural Characterization of Flavonoid Glycosides from Leaves of Wheat (Triticum aestivum L.) Using LC/MS/MS Profiling of the Target Compounds. Journal of Mass Spectometry, 48, 329-339.

[11]   Paul, P.A., Lipps, P.E., Hershman, D.E., McMullan, M.P., Draper, M.A. and Madden, L.V. (2008) Efficacy of Triazole-Based Fungicides for Fusarium Head Blight and Deoxynivalenol Control in Wheat: A Multivariate Meta-Analysis. Phytopathology, 98, 999-1011.

[12]   Yoshida, M., Nakajima, T., Tomimura, K., Suzuki, F., Arai, M. and Miyasaka, A. (2012) Effect of the Timing of Fungicide Application on Fusarium Head Blight and Mycotoxin Contamination in Wheat. Plant Disease Journal, 96, 845-851.