Rajesh Singh Pawar, Ankit Jain, Preeti Sharma, Pradeep Kumar Chaurasiya, Pradeep Kumar Singour

Abstract: The present study was undertaken to evaluate in-vitro antioxidant and anthelmintic activity of ethanolic and aqueous extract from whole plant Sida cordifolia Linn (Malvaceae). The antioxidant activities are evaluated by various antioxidant assays like α, α-Diphenyl-β-picrylhydrazyl (DPPH) free radical scavenging, total reducing power, nitric oxide scavenging and hydrogen peroxide scavenging. The various antioxidant activities were compared to standard antioxidants such as ascorbic acid. The antioxidant activity of ethanolic extract is almost quantitatively equivalent to that of the standards used, ascorbic acid. The further anthelmintic activity of whole plant is investigated through using Indian earthworm (Pheretima posthuma) showed that it is one of the most important local medicinal plants both for ritual and ethnomedical practices. Various concentrations of ethanol and aqueous extract (10, 20, 30, 40 mg/ml) of whole plant of Sida cordifolia Linn were tested in the bioassay, which involve determination of time of paralysis of the worms. Albendazole was included as reference standard. The most activity was observed with aqueous extract as compared to standard drug. The results from the above studies indicate that plant Sida cordifolia Linn. possesses potent antioxidant and anthelmintic activity.

Keywords: Albendazole, Anthelmintic, Antioxidant, Ascorbic acid, DPPH. Pheretima Posthuma, Sida cordifolia Linn

Cite this paper: nullR. Pawar, A. Jain, P. Sharma, P. Chaurasiya and P. Singour, "In Vitro Studies on Sida Cordifolia Linn for Anthelmintic and Antioxidant Properties," Chinese Medicine, Vol. 2 No. 2, 2011, pp. 47-52. doi: 10.4236/cm.2011.22009.

References

[1]   J. B. Harbone, “Phytochemical Methods: A Guide to Modern Techniques of Analysis,” Chapman and Hall Publishers, London, 1973, pp. 4-7.

[2]   P. K. Mukherjee, “Quality Control of Herbal Drugs,” 1st Edition, Bussiness Horizon, Pharmaceutical Publishers, New Delhi, 2002, pp. 380-422.

[3]   G. W. Thorn, R. D. Adams, E. Braunwald, K. J. Isselbacher and R. G. Peterdrof, “Harrison’s Principles of Internal medicine,” McGraw Hill Co., New York, 1977, pp. 1088-1089.

[4]   Z. Vigar, “Atlas of Medicinal Parasitology,” P. G. Publication House, Singapore, 1984, pp. 216-217.

[5]   S. A. Nirmal, G. Malwadkar and R. B. Laware, “Anthelmintic Activity of Pongamia Glabra,” Songklanakarin Journal of Science and Technology, Vol. 29, No. 3, 2007, pp. 755-757.

[6]   H. S. Ansari, “Essentials of Pharmacognosy,” Nirali Prakashan, Delhi, 2001, pp. 588-591.

[7]   K. R. Khandelwal, “Practical Pharmacognosy,” Ninth Edition, Nirali prakashan, Delhi, 2002, pp. 149-153.

[8]   V. D. Rangari, “Pharmacognosy and Phytochemistry, Part II,” First Edition, 2009, pp. 274-275.

[9]   The Indian Materia Medica. “With Ayurvedic, Unani and Home Remedies,” Vol. 3, 1993, pp. 1134-1137.

[10]   Pole, Sebastian, “Ayurvedic Medicine,” Elsevier Health Sciences, 2006: 137.

[11]   S. P. Agharkar, “Medicinal Plants of Bombay Presidency. Pbl,” Scientific Publishers, Jodhpur, 1991, pp. 194-195.

[12]   C. I. F. Franco, L. C. S. L. Morais, L. J. Quintans-Junior, R. N. Almeida and A. R. Antoniolli, “CNS Pharmacological Effects of the Hydroalcoholic Extract of Sida Cordifolia L. Leaves,” Journal of Ethnopharmacology, Vol. 98, No. 3, April 2005, pp. 275-279. doi:10.1016/j.jep.2005.01.008

[13]   V. R. Kanth and P. V. Diwan, “Analgesic, Antiinflammatory and Hypoglycaemic Activities of Sida Cordifolia,” Phytotherapy Research, Vol. 13, No. 1, 1999, pp. 75-77. doi:10.1002/(SICI)1099-1573(199902)13:1<75::AID-PTR387>3.3.CO;2-6

[14]   S. B. Kosalge and R. A. Fursul, “Investigation of in Vitro Anthelmintic Activity of Thespesia Lampas (cav.),” Easian Journal of Pharmaceutical and Clinical Research, Vol. 2, No. 2, April-June 2009, pp. 69-71.

[15]   R. S. Kumar and S. H. Mishra, “Anti-Inflammatory and Hepatoprotective Activities of Sida Rhombifolia Linn,” Indian Journal of Pharmacology, Vol. 29, No. 2, 1997, pp. 110-116.

[16]   W. B. William, M. E. Cuvelier and C. Berset, “Use of Free Radical Method to Evaluate Antioxidant Activity,” Food Science Technology, Vol. 28, No. 1, 1995, pp. 25-30.

[17]   M. Oyaizu, “Studies on Products of Browning Reactions: Antioxidant Activities of Products of Browning Reaction Prepared from Glucose Amine,” Japanese Journal of Nutrition, Vol. 44. 1986, pp. 307-315.

[18]   M. P. Madan, G. Raghavan, A. K. Singh and P. Palpu, “Free Radical Scavenging Potential of Saussare Costus,” Acta Pharma, Vol. 55, 2005, pp. 297-304.

[19]   R. J. Ruch, S. U. Chug and J. E. Klaunig, “Spin Trapping of Superoxide and Hydroxyl Radicals,” Methods in Enzymology, Vol. 105, 1984, pp. 198-209. doi:10.1016/S0076-6879(84)05026-6

[20]   www.docstoc.com/docs//38801351/sidacordifolia.

[21]   R. K. Sutradhar, A. K. M. Rahman, M. U. Ahmad and S. C. Bachar, “Bioactive Flavones of Sida Cordifolia,” Phytochemistry Letters, Vol. 1, No. 4, December 2008, pp. 179-182.