First Otoliths/Collagen/Bacterial Cellulose Nanocomposites as a Potential Scaffold for Bone Tissue Regeneration
Author(s)
G.M Olyveira*,
Daisy Pereira Valido,
L.M.M. Costa,
Plácia Barreto Prata Gois,
Lauro Xavier Filho,
Pierre Basmaji
ABSTRACT
In the present work, we report the first bionanocomposite material formed by otoliths/ collagen/ bacterial cellulose (BC) networks (OCBC). This biomaterial is an osteoinductor or be, stimulates the bone regeneration, enabling bigger migration of the cells for formation of the bone tissue regeneration mainly because nanotolith are rich in minerals considered essential to the bone mineralization process on a protein matrix (otolin). The objective in this study was to analyze the regeneration capacity of bone defects treated with this bionanocomposite. Histological experiments shows bone tissue formation with high regularity, higher osteoblast activity and osteo-reabsorption activities areas. The results suggest the potential for this new biomaterial as a scaffold for bone tissue regeneration.
In the present work, we report the first bionanocomposite material formed by otoliths/ collagen/ bacterial cellulose (BC) networks (OCBC). This biomaterial is an osteoinductor or be, stimulates the bone regeneration, enabling bigger migration of the cells for formation of the bone tissue regeneration mainly because nanotolith are rich in minerals considered essential to the bone mineralization process on a protein matrix (otolin). The objective in this study was to analyze the regeneration capacity of bone defects treated with this bionanocomposite. Histological experiments shows bone tissue formation with high regularity, higher osteoblast activity and osteo-reabsorption activities areas. The results suggest the potential for this new biomaterial as a scaffold for bone tissue regeneration.
KEYWORDS
bacterial cellulose, natural composites, bionanocomposites, tissue engineering, bone tissue regeneration
bacterial cellulose, natural composites, bionanocomposites, tissue engineering, bone tissue regeneration
Cite this paper
nullG. Olyveira, D. Valido, L. Costa, P. Gois, L. Xavier Filho and P. Basmaji, "First Otoliths/Collagen/Bacterial Cellulose Nanocomposites as a Potential Scaffold for Bone Tissue Regeneration," Journal of Biomaterials and Nanobiotechnology, Vol. 2 No. 3, 2011, pp. 239-243. doi: 10.4236/jbnb.2011.23030.
nullG. Olyveira, D. Valido, L. Costa, P. Gois, L. Xavier Filho and P. Basmaji, "First Otoliths/Collagen/Bacterial Cellulose Nanocomposites as a Potential Scaffold for Bone Tissue Regeneration," Journal of Biomaterials and Nanobiotechnology, Vol. 2 No. 3, 2011, pp. 239-243. doi: 10.4236/jbnb.2011.23030.
References
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[1] Taton TA (2001) Nanotechnology: Boning up on biology. Nature 412: 491-492. [2] Kim BS, Baez CE, Atala A (2000) Biomaterials for tissue engineering. World J Urol 18: 2-9. [3] Basmaji P et al. Brazilian patent number: PI0604760-2. [4] Tuzlakoglu K, Bolgen N, Salgado AJ et AL (2005) Nano- and micro-fiber combined scaffolds: A new architecture for bone tissue engineering. J Mater Sci: Mater Med16: 1099-1104. [5] Czaja, W, Krystynowicz A, Bielecki S et a (2006) Microbial cellulose-the natural power to heal wounds. Biomaterials 27: 145-151. [6] Dahlin C, Linde A, Gottlow J et al (1998) Healing of bone defects by guided tissue regeneration. Plast Reconstr Surg 81: 672-676. [7] Macedo NL, Matuda FS, De Macedo LGS et al. (2004) Evaluation of two membranes in guided bone tissue regeneration: histological study in rabbits. Braz J Oral Sci 3: 395-400. [8] Hunziker EB (2001) Articular cartilage repair: basic science and clinical progress. A review of the current status and prospects. Osteoarthr Cartilage 10: 432-463. [9] Svensson A, Nicklasson E, Harrah T et al (2005) Bacterial cellulose as a potential scaffold for tissue engineering of cartilage. Biomaterials 26: 419-431. [10] Aslan M, Simsek G, Dayl E (2004) Guided bone regeneration (GBR) on healing bone defects: a histological study in rabbits. J Contemp Dent Pract 2: 114-123. [11] Carvalho RS, Nelson D, Keldernian H et al (2003) Guided bone regeneration to repair an osseous defect. Am J Orthod Dentofacial Orthop 123: 455-467. [12] Iamaguti LS, Brand?o CVS, Pellizzon CH et al (2008) Análise histológica e morfométrica do uso de membrana biossintética de celulose em trocleoplastia experimental de c?es. Pesq Vet Bras 28:195-200. [13] Helenius G, B?ckdahl H, Bodin A et al (2006) In vivo biocompatibility of bacterial cellulose. J Biomed Mater Res- A76: 431-438. [14] Grande CJ, Torres FG, Gomez CM et al (2009) Development of self-assembled bacterial cellulose–starch nanocomposites. Mater Sci Eng-C 29: 1098-1104. [15] Grande CJ, Torres FG, Gomez CM et al. (2009) Nanocomposites of bacterial cellulose/hydroxyapatite for biomedical applications. Acta Biomaterialia 5: 1605-1615. [16] Bhattarai SJ, Bhattarai N, Yi HK et al (2005) Novel biodegradable electrospun membrane: scaffold for tissue engineering. Biomaterials 25: 2595-602. [17] Yang S, Leong KF, Du Z et al. (2001) The design of scaffolds for use in tissue engineering Part I. Traditional factors Tissue Eng 7: 679-89. [18] Chen GP, Ushida T, Tateishi T (2000) Hybrid biomaterials for tissue engineering: a preparative method for PLA or PLGA-collagen hybrid sponges. Adv Mater 12: 455-467. [19] Geiger M, Li RH, Friess W (2003) Collagen sponges for bone regeneration with rhBMP-2. Adv Drug Deliv Rev 55: 1613-1629. [20] Meinel L, Langer R, Vunjak-Novakovic G et al. (2004) Bone tissue engineering using human mesenchymal stem cells: effects of scaffold material and medium flow. Ann Biomed Eng 32: 112-122. [21] Korkusuz F, Korkusuz P, Hasirci V(2004) In vivo tissue engineering of bone using poly(3-hydroxybutyric acid-co-3-hydroxyvaleric acid) and collagen scaffolds. Tissue Eng 10: 1234-1250. [22] Klemm D, Heublein B, Fink HP et al (2005) Cellulose: fascinating biopolymer and sustainable raw material A. Bohn, Polymer Science 44: 3358-3393. [23] Valido DP, Bastos TS, Albuquerque-Júnior RLC et al. (2010) Estudo preliminar da utiliza??o de otólitos de Cynoscion acoupa sobre o processo de neoforma??o óssea em ratos J Bras Patol Med Lab 46: 315-322. [24] Luginbuehl V, Wenk E, Koch A et al. (2005) Insulin-like growth factorI-releasing alginate-tricalciumphosphate composites for bone regeneration. Pharm Res 22:940-950 [25] Seol YJ, Lee JY, Park YJ et al. (2004) Chitosan sponges as tissue engineering scaffolds for bone formation. Biotechnol Lett 26: 1037-1041.