Back
 AJPS  Vol.5 No.24 , November 2014
Comparative Analysis of Three Torbangun Clones (Plectranthus amboinicus (Lour.) Spreng) Based on Phenotypic Characteristics and Phenolic Content
Nuri Andarwulan1,2*, Nancy Dewi Yuliana1, Elva Hasna1, Sandra Arifin Aziz3, Tim D. Davis4
Show more
Abstract: Torbangun is a folk herb that has been used especially as a breast milk stimulant in North Sumatra, Indonesia. Plant bioactive compound composition is influenced by many factors such as genotype, geographical origin, and environment. Therefore, identifying plant clones with superior chemical composition is a necessity for optimal large-scale production. In this study, three clones of torbangun (hereafter referred to as A, B, and C clones) were analyzed through their phenotypic and foliar phenolic characteristics. The phenotypic results showed that the A clone was distinct from the B and C clones. Nevertheless, the result of multivariate analysis using phenolic data showed that these three clones had three distinct patterns of phenolic compounds. The B clone torbangun was identified as the best clone to be used in larger production scale due to its highest quantity of most phenolic compounds.
Keywords: Bioactive Compounds, Plant Morphology, Multivariate Analysis
Cite this paper: Andarwulan, N. , Yuliana, N. , Hasna, E. , Aziz, S. and Davis, T. (2014) Comparative Analysis of Three Torbangun Clones (Plectranthus amboinicus (Lour.) Spreng) Based on Phenotypic Characteristics and Phenolic Content. American Journal of Plant Sciences, 5, 3673-3683. doi: 10.4236/ajps.2014.524383.
References

[1]   Dalimartha, S. (2008) Daun Jinten (Coleus amboinicus Lour.). In: Dalimartha, S., Ed., Atlas Tumbuhan Obat Indonesia Jilid 5, Pustaka Bunda, Jakarta, 25-28.

[2]   Manjamalai, A., Narala, Y., Haridas, A. and Grace, V.M.B. (2011) Antifungal, Anti-Inflammatory and GC-MS Analysis of Methanolic Extract of Plectranthus amboinicus leaf. International Journal of Current Pharmaceutical Research, 2, 129-136.

[3]   Prameela, T.S. and Oommen, P.S. (2011) Phytochemical Screening, Antimicrobial, and Anthelminthic Studies on Coleus aromaticus (Benth). Journal of Pharmaceutical Research and Development, 4, 93-103.

[4]   Roshan, P., Naveen, M., Sudarshan, S., Ravindra, G., Basant, K. and Vidyanand, P. (2010) Antioxidant Potential of Leaves of Plectranthus amboinicus (Lour) Spreng. Der Pharmacia Lettre, 4, 240-245.

[5]   Damanik, R., Wahlqvist, M.L. and Wattanapenpaiboon, N. (2006) Lactagogue Effects of Torbangun, a Bataknese Traditional Cuisine. Asia Pacific Journal of Clinical Nutrition, 15, 267-274.

[6]   Sathasivam, A. and Elangovan, K. (2011) Evaluation of Phytochemical and Antibacterial Activity of Plectranthus amboinicus. International Journal of Research in Ayurveda and Pharmacy, 1, 292-294.

[7]   Kundu, K., Khare, R.S. and Banerjee, S. (2011) Coleus aromaticus Benth—A Nutritive Medicinal Plant of Potential Therapeutic Value. International Journal of Pharma Bioscience and Technology, 3, 488-500.

[8]   Thenmozhi, S., Bhuvana, M. and John, S.A. (2011) Screening of Antimicrobial and Phytochemical Investigation of Coleus aromaticus (Benth) Leaf against Five Respiratory Pathogens. Journal of Pharmacy Research, 7, 2261-2262.

[9]   Jayadeepa, R.M., Anupama, S. and Aswathy, R.M. (2011) Insilico Techniques for the Identification of Novel Natural Compounds for Secreting Human Breast Milk. WebmedCentral Bioinformatics, 8, 1-6.

[10]   Hansen, M. and Wold, A.B. (2010) Contents of Bioactive Compounds in Food Plants as Affected by Traditional Breeding and Environmental Factor. Proceedings from a Symposium Held at the Norwegian Academy of Science and Letters, Oslo, 13-14 November 2008, 212-222.

[11]   Anonymous (2006) Multi-and Megavariate Analysis Part 1: Basic Principles and Application. Umetrics AB, Malmo.

[12]   Bioversity International (2007) Developing Crop Descriptor Lists, Bioversity Technical Bulletin Series. Bioversity International, 13, 5-71.

[13]   Shetty, K., Curtis, O.F., Levin, R.E., Witkowsky, R. and Ang, W. (1995) Prevention of Vitrification Associated with in Vitro Shoot Culture of Oregano (Origanum vulgare) by Pseudomonas spp. Journal of Plant Physiology, 147, 447-451.

[14]   Sims, D.A. and Gamon, J.A. (2002) Relationships between Leaf Pigment Content and Spectral Reflectance across a Wide Range of Species, Leaf Structures and Developmental Stages. Remote Sensing of Environment, 81, 337-354.
http://dx.doi.org/10.1016/S0034-4257(02)00010-X

[15]   Hertog, M.G.L., Hollman, P.C.H. and Venema, D.P. (1992) Optimization of a Quantitative HPLC Determination of Potentially an Ticarcinogenic Flavonoids in Vegetables and Fruits. Journal of Agricultural and Food Chemistry, 40, 1591-1598. http://dx.doi.org/10.1021/jf00021a023

[16]   Marinova, D., Ribarova, F. and Atanassova, M. (2005) Total Phenolics and Total Flavonoids in Bulgarian Fruits and Vegetables. Journal of the University of Chemical Technology and Metallurgy, 40, 255-260.

[17]   Andarwulan, N., Batari, R., Sandrasari, D.A., Bolling, B. and Wijaya, H. (2010) Flavonoid Content and Antioxidant Activity of Vegetables from Indonesia. Food Chemistry, 121, 1231-1235.
http://dx.doi.org/10.1016/j.foodchem.2010.01.033

[18]   Andarwulan, N., Kurniasih, D., Apriady, R.A., Rahmat, H., Roto, A.V. and Bolling, B.W. (2012) Polyphenols, Carotenoids, and Ascorbic Acid in Underutilized Vegetables. Journal of Functional Foods, 4, 339-347.
http://dx.doi.org/10.1016/j.jff.2012.01.003

[19]   Hertog, M.G.L., Hollman, P.C.H. and Katan, M.B. (1992) Content of Potentially Anticarcinogenic Flavonoids of 28 Vegetables and 9 Fruits Commonly Consumed in the Netherlands. Journal of Agricultural and Food Chemistry, 40, 2379-2383. http://dx.doi.org/10.1021/jf00024a011

[20]   Nuutila, A.M., Kammiovirta, K. and Oksman-Caldentey, K.M. (2002) Comparison of Methods for the Hydrolysis of Flavonoids and Phenolic Acids from Onion and Spinach for HPLC Analysis. Food Chemistry, 76, 519-525.

[21]   Bae, H., Jayaprakasha, G.K., Jifon, J. and Patil, B.S. (2012) Extraction Efficiency and Validation of an HPLC Method for Flavonoid Analysis in Peppers. Food Chemistry, 130, 751-758. http://dx.doi.org/10.1016/j.foodchem.2011.07.041

[22]   Lee, H.S. (2000) HPLC Analysis of Phenolic Compounds. In: Nollet, L.M.L., Ed., Food Analysis by HPLC, 2nd Edition, Revised and Expanded, Marcel Dekker Inc., New York, 775-824.

 
 
Top