GSC  Vol.4 No.4 , November 2014
Efficient Extraction of Agarose from Red Algae Using Ionic Liquids
ABSTRACT
We explored the possibility of using ionic liquids (ILs) as medium for efficient extraction of agarose via dissolution of red algae under varying conditions of heating or microwave irradiation. As compared to conventional methods, a very high extraction yield of good quality agarose (as high as 39 wt%) could be achieved depending upon the nature of used IL and applied experimental conditions. Purity of extracted agarose was confirmed from various spectral and analytical techniques, such as 1H and 13C NMR, FTIR, circular dichroism (CD), gel permeation chromatography (GPC) and thermogravimetric analysis (TGA). The physicochemical properties, such as gelling or melting temperature, viscosity and gel strength of extracted agarose hydrogels have been measured and compared with the agarose obtained from similar source reported in the literature. ILs were recovered after the extraction of agarose and were reused for further extraction experiments. % Recycling and extraction ability of recycled ILs in different cycles have been measured. The developed extraction process of utilizing ILs as medium is easy, simple and highly efficient as compared to the conventional methods of agarose extraction from algae.

Cite this paper
Trivedi, T. and Kumar, A. (2014) Efficient Extraction of Agarose from Red Algae Using Ionic Liquids. Green and Sustainable Chemistry, 4, 190-201. doi: 10.4236/gsc.2014.44025.
References
[1]   Schuerch, C. (1986) Polysaccharides in Encyclopedia of Polymer Science and Engineering. 2nd Edition, John Wiley & Sons, New York, 87-162.

[2]   Gellerstedt, G., Hon, D.N.-S., Shiraishi, N. and Dekker, M. (2001) Pulping Chemistry in Wood and Cellulosic Chemistry. Marcel Dekker, New York, 859-905.

[3]   Huddleston, J.G., Willauer, H.D., Swatloski, R.P., Visser, A.E. and Rogers, R.D. (1998) Room Temperature Ionic Liquids as Novel Media for “Clean” Liquid—Liquid Extraction. Chemical Communication, 135-136.

[4]   Rogers, R.D., Seddon, K.R. and Volkov, S. (2002) Green Industrial Applications of Ionic Liquids. NATO Science Series, Kluwer, Dordre CH.

[5]   Swatloski, R.P., Spear, S.K., Holbrey, J.D. and Rogers, R.D. (2002) Dissolution of Cellulose with Ionic Liquids. Journal of American Chemical Society, 124, 4974-4975.
http://dx.doi.org/10.1021/ja025790m

[6]   Brandt, A., Hallett, J.P., Leak, D.J., Murphy, R.J. and Welton, T. (2010) The Effect of the Ionic Liquid Anion in the Pretreatment of Pine Wood Chips. Green Chemistry, 12, 672-679.
http://dx.doi.org/10.1039/b918787a

[7]   Wang, H., Gurau, G. and Rogers, R.D. (2012) Ionic Liquid Processing of Cellulose. Chemical Society Review, 41, 1519-1537.
http://dx.doi.org/10.1039/c2cs15311d

[8]   Da Costa Lopes, A.M., João, K.G., C Morais, A.R., Bogel-Lukasik, E. and Bogel-Lukasik, R. (2013) Ionic Liquids as Tool for Lignocellulosic Biomass Fractionation. Sustainable Chemical Processes, 13, 1-31.
http://dx.doi.org/10.1186/2043-7129-1-3

[9]   Manic, M.S., Visak, V.N., Ponte, M.N.D. and Visak, Z.P. (2011) Extraction of Free Fatty Acids from Soybean Oil Using Ionic Liquids or Poly(ethyleneglycol)s. AIChE Journals, 57, 1377-1355.
http://dx.doi.org/10.1002/aic.12349

[10]   Usuki, T., Yasuda, N., Fujita, M.Y. and Rikukawa, M. (2011) Extraction and Isolation of Shikimic Acid from Ginkgo biloba Leaves Utilizing an Ionic Liquid That Dissolves Cellulose. Chemical Communication, 47, 10560-10562.
http://dx.doi.org/10.1039/c1cc13306c

[11]   Ressmann, A.K., Gaertner, P. and Bica, K. (2011) From Plant to Drug: Ionic Liquids for the Reactive Dissolution of Biomass. Green Chemistry, 13, 1442-1447.
http://dx.doi.org/10.1039/c1gc15058h

[12]   Chi, Y.S., Zhang, Z.D., Li, C.P., Liu, Q.S., Yan, P.F. and Urs, W.-B. (2011) Microwave-Assisted Extraction of Lactones from Ligusticum chuanxiong Hort. Using Protic Ionic Liquids. Green Chemistry, 13, 666-670.
http://dx.doi.org/10.1039/c0gc00864h

[13]   Chowdhury, S.A., Vijayaraghavan, R. and MacFarlane, D.R. (2010) Distillable Ionic Liquid Extraction of Tannins from Plant Materials. Green Chemistry, 12, 1023-1028.
http://dx.doi.org/10.1039/b923248f

[14]   Cláudio, A.F.M., Ferreira, A.M., Freire, M.G. and Coutinho, J.A.P. (2013) Enhanced Extraction of Caffeine from Guaraná Seeds Using Aqueous Solutions of Ionic Liquids. Green Chemistry, 15, 2002-2010.
http://dx.doi.org/10.1039/c3gc40437d

[15]   Fort, D.A., Remsing, R.C., Swatloski, R.P., Moyna, P., Moyna, G. and Rogers, R.D. (2007) Can Ionic Liquids Dissolve Wood? Processing and Analysis of Lignocellulosic Materials with 1-n-Butyl-3-Methylimidazolium Chloride. Green Chemistry, 9, 63-69.
http://dx.doi.org/10.1039/b607614a

[16]   Sun, N., Rahman, M., Qin, Y., Maxim, M.L., Rodrìgues, H. and Rogers, R.D. (2009) Complete Dissolution and Partial Delignification of Wood in the Ionic Liquid 1-Ethyl-3-Methylimidazolium Acetate. Green Chemistry, 11, 646-655.
http://dx.doi.org/10.1039/b822702k

[17]   Wang, X., Li, H., Cao, Y. and Tang, Q. (2011) Cellulose Extraction from Wood Chip in an Ionic Liquid 1-Allyl-3- Methylimidazolium Chloride (AmimCl). Bioresource Technology, 102, 7959-7965.
http://dx.doi.org/10.1016/j.biortech.2011.05.064

[18]   Hamada, Y., Yoshida, K., Asai, R.-I., Hayase, S., Nokami, T., Izumib, S. and Itoh, T. (2013) A Possible Means of Realizing a Sacrifice-Free Three Component Separation of Lignocellulose from Wood Biomass Using an Amino Acid Ionic Liquid. Green Chemistry, 15, 1863-1868.
http://dx.doi.org/10.1039/c3gc40445e

[19]   Lan, W., Liu, C.-F. and Sun, R.-C. (2011) Fractionation of Bagasse into Cellulose, Hemicelluloses, and Lignin with Ionic Liquid Treatment Followed by Alkaline Extraction. Journal of Agriculture and Food Chemistry, 59, 8691-8701.
http://dx.doi.org/10.1021/jf201508g

[20]   Tan, S.S.Y., MacFarlane, D.R., Upfal, J., Edye, L.A., Doherty, W.O.S., Patti, A.F., Pringle, J.M. and Scott, J.L. (2009) Extraction of Lignin from Lignocellulose at Atmospheric Pressure Using Alkylbenzenesulfonate Ionic Liquid. Green Chemistry, 11, 339-345.
http://dx.doi.org/10.1039/b815310h

[21]   Hossain, M.M. and Aldous, L. (2012) Ionic Liquids for Lignin Processing: Dissolution, Isolation and Conversion. Australian Journal of Chemistry, 65, 1465-1477.
http://dx.doi.org/10.1071/CH12324

[22]   Xin, Q., Pfeiffer, K., Prausnitz, J.M., Clark, D.S. and Blanch, H.W. (2012) Extraction of Lignins from Aqueous-Ionic Liquid Mixtures by Organic Solvents. Biotechnology and Bioengineering, 109, 346-352.
http://dx.doi.org/10.1002/bit.24337

[23]   Pinkert, A., Goeke D.F., Marsh, K.N. and Pang, S. (2011) Extracting Wood Lignin without Dissolving or Degrading Cellulose: Investigations on the Use of Food Additive-Derived Ionic Liquids. Green Chemistry, 13, 3124-3136.
http://dx.doi.org/10.1039/c1gc15671c

[24]   Prado, R., Erdocia, X. and Labidi, J. (2013) Lignin Extraction and Purification with Ionic Liquids. Journal of Chemical Technology and Biotechnology, 88, 1248-1257.
http://dx.doi.org/10.1002/jctb.3965

[25]   Meng, L., Kang, S., Zhang, X., Wu, Y. and Sun, R. (2012) Comparative Characterization of Lignins Extracted from Cotton Stalk Based on Complete Dissolution in Different Systems. Industrial & Engineering Chemistry Research, 51, 9858-9866.
http://dx.doi.org/10.1021/ie301118e

[26]   Wang, Y.-X. and Cao, X.-J. (2012) Extracting Keratin from Chicken Feathers by Using a Hydrophobic Ionic Liquid. Process Biochemistry, 47, 896-899.
http://dx.doi.org/10.1016/j.procbio.2012.02.013

[27]   Huang, G.L., Shi, J., Zhang, K. and Huang, X.L. (2012) Application of Ionic Liquids in the Microwave Assisted Extraction of Pectin from Lemon Peels. Journal of Analytical Methods in Chemistry, 2012, Article ID: 302059.
http://dx.doi.org/10.1155/2012/302059

[28]   Qin, Y., Lu, X., Sun, N. and Rogers, R.D. (2010) Dissolution or Extraction of Crustacean Shells Using Ionic Liquids to Obtain High Molecular Weight Purified Chitin and Direct Production of Chitin Films and Fibers. Green Chemistry, 12, 968-971.
http://dx.doi.org/10.1039/c003583a

[29]   Barber, P.S., Griggs, C.S., Bonner, J.R. and Rogers, R.D. (2013) Electrospinning of Chitin Nanofibers Directly from an Ionic Liquid Extract of Shrimp Shells. Green Chemistry, 15, 601-607.
http://dx.doi.org/10.1039/c2gc36582k

[30]   Ferreira, R., Garcia, H., Sousa, A.F., Petkovic, M., Lamosa, P., Freire, C.S.R., Silvestre, A.J.D., Rebelo, L.P.N. and Pereira, C.S. (2012) Suberin Isolation from Cork Using Ionic Liquids: Characterisation of Ensuing Products. New Journal of Chemistry, 36, 2014-2024.
http://dx.doi.org/10.1039/c2nj40433h

[31]   Teixeira, R.E. (2012) Energy-Efficient Extraction of Fuel and Chemical Feedstocks from Algae. Green Chemistry, 14, 419-427.
http://dx.doi.org/10.1039/c2gc16225c

[32]   Kim, Y.-H., Choi, Y.-K., Park, J., Lee, S., Yang, Y.-H., Kim, H.J., Park, T.-J., Kim, Y.H. and Lee, S.H. (2012) Ionic Liquid-Mediated Extraction of Lipids from Algal Biomass. Bioresource Technology, 109, 312-315.
http://dx.doi.org/10.1016/j.biortech.2011.04.064

[33]   Abe, M., Fukaya, Y. and Ohno, H. (2010) Extraction of Polysaccharides from Bran with Phosphonate or Phosphinate Derived Ionic Liquids under Short Mixing Time and Low Temperature. Green Chemistry, 12, 1274-1280.
http://dx.doi.org/10.1039/c003976d

[34]   Fujita, K., Kobayanshi, D., Nakamura, N. and Ohno, H. (2013) Direct Dissolution of Wet and Saliferous Marine Microalgae by Polar Ionic Liquids without Heating. Enzyme Microbial Technology, 52, 199-202.
http://dx.doi.org/10.1016/j.enzmictec.2012.12.004

[35]   Meena, R., Siddhanta, A.K., Prasad, K., Ramavat, B.K., Eswaran, K., Thiruppathi, S., Ganesan, M., Mantri, V.A. and Rao, P.V.S. (2007) Preparation, Characterization and Benchmarking of Agarose from Gracilaria dura of Indian Waters. Carbohydrate Polymers, 69, 179-188.
http://dx.doi.org/10.1016/j.carbpol.2006.09.020

[36]   Craigie, J.S. and Leigh, C. (1978) Carrageenans and Agars. In: Hellebust, J.A. and Craigie, J.S., Eds., Handbook of Phycological Methods, Cambridge University Press, Cambridge, 109-131.

[37]   Wolnik, K.A. (1988) Inductively Coupled Plasma-Emission Spectrometry. Methods Enzymology, 158, 190-205.
http://dx.doi.org/10.1016/0076-6879(88)58056-4

[38]   Muhammad, N., Man, Z., Bustam, M.A., Mutalib, M.I.A., Wilfred, C.D. and Rafiq, S. (2011) Dissolution and Delignification of Bamboo Biomass Using Amino Acid-Based Ionic Liquid. Applied Biochemistry and Biotechnology, 165, 998-1009. http://dx.doi.org/10.1007/s12010-011-9315-y

[39]   Brandt, A., Gräsvik, J., Hallett, J.P. and Welton, T. (2013) Deconstruction of Lignocellulosic Biomass with Ionic Liquids. Green Chemistry, 15, 550-583.
http://dx.doi.org/10.1039/c2gc36364j

[40]   Christiaen, D. and Bodard, M. (1983) Spectroscopieinfrarouge de films d’agar de Gracilariaverrucosa (Huds) Papenfuss. Botanica Marina, 26, 425-427.

[41]   Singh, T., Trivedi, T.J. and Kumar, A. (2010) Dissolution, Regeneration and Ion-Gel Formation of Agarose in Room-Temperature Ionic Liquids. Green Chemistry, 12, 1029-1035.
http://dx.doi.org/10.1039/b927589d

[42]   Trivedi, T.J., Srivastava, D.N., Rogers, R.D. and Kumar, A. (2012) Agarose Processing in Protic and Mixed Protic- Aprotic Ionic Liquids: Dissolution, Regeneration and High Conductivity, High Strength Ionogels. Green Chemistry, 14, 2831-2839.
http://dx.doi.org/10.1039/c2gc35906e

[43]   Trivedi, T.J., Rao, S.K. and Kumar, A. (2014) Facile Preparation of Agarose-Chitosan Hybrid Materials and Nanocomposite Ionogels Using an Ionic Liquid via Dissolution, Regeneration and Sol-Gel Transition. Green Chemistry, 16, 320-330.
http://dx.doi.org/10.1039/c3gc41317a

[44]   Rees, D.A. (1981) Polysaccharide Shapes and Their Interactions—Some Recent Advances. Pure & Applied Chemistry, 53, 1-14.
http://dx.doi.org/10.1351/pac198153010001

 
 
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