AJAC  Vol.2 No.2 , May 2011
Voltammetric Determination of Vitamin B6 at Glassy Carbon Electrode Modified with Gold Nanoparticles and Multi-Walled Carbon Nanotubes
Abstract: In this work, the gold nanoparticles (Au NPs)/multi-walled carbon nanotubes (MWCNTs) composite film modified glassy carbon electrode (GCE) was fabricated, and scanning electron microscopy (SEM) was used to investigate the assemble process of the composite film. In pH 7.0 PBS, a oxidation peak of the vitamin B6 (VB6) was only observed at composite film modified electrode. Under the optimized conditions, the current intensity was linear with the concentrations of VB6 in the range of 1.59 to 102.74 μg●mL–1 with a detection limit of 0.53 μg●mL–1 (S/N = 3). The modified electrode had been applied in medication analysis, and obtained good results.
Cite this paper: nullY. Zhang and Y. Wang, "Voltammetric Determination of Vitamin B6 at Glassy Carbon Electrode Modified with Gold Nanoparticles and Multi-Walled Carbon Nanotubes," American Journal of Analytical Chemistry, Vol. 2 No. 2, 2011, pp. 194-199. doi: 10.4236/ajac.2011.22022.

[1]   G. Melentyeva and L. Antonova, “Pharmaceutical Chemistry,” Mir Publishers, Moscow, 1988, pp. 375-393.

[2]   García, S. Blázquez, M. P. S. Andrés and S. Vera, “Determination of Thiamine, Riboflavin and Pyridoxine in Pharmaceuticals by Synchronous Fluorescence Spectrometry in Organized Media,” Analytica Chimica Acta, Vol. 434, No. 2, May 2001, pp. 93-99.

[3]   P. O. Barrales, M. L. F. Córdova and A. M. Díaz, “Microdetermination of Vitamin B1 in the Presence of Vitamins B2, B6, and B12 by Solid-Phase UV Spectrophotometry,” Analytical Chemistry, Vol. 70, No. 2, January 1998, pp. 271-275.

[4]   W. Y. Hou, H. M. Ji and E. K. Wang, “Liquid Chromatography of Vitamin B6 with Electrochemical Detection Using a Carbon Fibre Electrode,” Analytica Chimica Acta, Vol. 230, March 1990, pp. 207-211. doi:10.1016/S0003-2670(00)82784-1

[5]   G. Chen, X. G. Ding, Z. G. Cao and J. N. Ye, “Determination of Melatonin and Pyridoxine in Pharmaceutical Preparations for Health-Caring Purposes by Capillary Electrophoresis with Electrochemical Detection,” Analytica Chimica Acta, Vol. 408, No. 1-2, March 2000, pp. 249-256. doi:10.1016/S0003-2670(99)00809-0

[6]   F. S. T. Marcos, S. Aline, C. M. Fernando, J. L. H. Marcolino, F. O. Fatibello and é. T. G. Cavalheiro, “Determination of Vitamin B6 (Pyridoxine) in Pharmaceutical Preparations by Cyclic Voltammetry at a Copper (II) Hexacyanoferrate (III) Modified Carbon Paste Electrode,” Journal of the Brazilian Chemical Society, Vol. 14, No. 2, April 2003, pp. 316-321. doi:10.1590/S0103-50532003000200021

[7]   F. S. T. Marcos, M. Glimaldo, E. R. Dockal and é. T. G. Cavalheiro, “Voltammetric Determination of Pyridoxine (vitamin B6) at a Carbon Paste Electrode Modified with Vanadyl (IV)-Salen Complex,” Analytica Chimica Acta, Vol. 508, No. 1, April 2004, pp. 79-85. doi:10.1016/j.aca.2004.03.021

[8]   R. Jiménez-Prieto, M. Silva and D. Pérez-Bendito D, “Analytical Assessment of the Oscillating Chemical Reactions by Use Chemiluminescence Detection,” Talanta, Vol. 44, No. 8, August 1997, pp. 1463-1472. doi:10.1016/S0039-9140(97)00047-7

[9]   Q. Hu, T. S. Zhou, L. Zhang, H. Li and Y. Z. Fang, “Separation and Determination of Three Water-Soluble Vitamins in Pharmaceutical Preparations and Food by Micellar Electrokinetic Chromatography with Ampero- metric Electrochemical Detection,” Analytica Chimica Acta, Vol. 437, No. 1, June 2001, pp. 123-129.

[10]   P. S?derhjelm and J. Lindquist, “Voltammetric Determination of Pyridoxine by Use of a Carbon Paste Electrode,” Analyst, Vol. 100, No. 1190, May 1975, pp. 349- 354. doi:10.1039/an9750000349

[11]   M. Y. Li, G. Q. Zhao, Z. L. Yue and S. S. Huang, “Sensor for Traces of Hydrogen Peroxide Using an Electrode Modified by Multiwalled Carbon Nanotubes, a Gold- Chitosan Colloid, and Prussian Blue,” Microchimica Acta, Vol. 167, No. 2, November 2009, pp. 167-172. doi:10.1007/s00604-009-0238-z

[12]   J. Z. Xu, J. J. Zhu, Q. Wu, Z. Hu and H. Y. Chen, “An Amperometric Biosensor Based on the Coimmobilization of Horseradish Peroxidase and Methylene Blue on a Carbon Nanotubes Modified Electrode,” Electroanalysis, Vol. 15, No. 3, March 2003, pp. 219-224. doi:10.1002/elan.200390027

[13]   L. Q. Jiang and L. Gao, “Modified Carbon Nanotubes: An Effective Way to Selective Attachment of Gold Nanoparticles,” Carbon, Vol. 41, No. 15, November 2003, pp. 2923-2929. doi:10.1016/S0008-6223(03)00339-7

[14]   L. Wang, S. J. Guo, L. J. Huang and S. J. Dong, “Alternate Assemblies of Polyelectrolyte Functionalized Carbon Nanotubes and Platinum Nanoparticles as Tunable Electrocatalysts for Dioxygen Reduction,” Electrochemistry Communications, Vol. 9, No. 4, April 2007, pp. 827- 832. doi:10.1016/j.elecom.2006.11.009

[15]   Y. Liu, S. Wu, H. X. Ju and L. Xu, “Amperometric Glucose Biosensing of Gold Nanoparticles and Carbon Nanotube Multilayer Membranes,” Electroanalysis, Vol. 19, No. 9, May 2007, pp. 986-992. doi:10.1002/elan.200603814

[16]   R. F. Gao and J. B. Zheng, “Amine-Terminated Ionic Liquid Functionalized Carbon Nanotube-Gold Nanoparticles for Investigating the Direct Electron Transfer of Glucose Oxidase,” Electrochemistry Communications, Vol. 11, No. 3, March 2009, pp. 608-611. doi:10.1016/j.elecom.2008.12.060

[17]   S. H. Lim, J. Wei and J. Y. Lin, “Electrochemical Genosensing Properties of Gold Nanoparticle-Carbon Nanotube Hybrid,” Chemical Physics Letters, Vol. 400, No. 4-6, December 2004, pp. 578-582. doi:10.1016/j.cplett.2004.11.023

[18]   Y. Zhang, T. F. Kang, Y. W. Wan and S. Y. Chen, “Gold Nanoparticles-Carbon Nanotubes Modified Sensor for Electrochemical Determination of Organophosphate Pesticides,” Microchimica Acta, Vol. 165, No. 3-4, January 2009, pp. 307-311. doi:10.1007/s00604-008-0134-y

[19]   L. Xiao, G. G. Wildgoose and R. G. Compton, “Sensitive Electrochemical Detection of Arsenic (III) Using Gold Nanoparticle Modified Carbon Nanotubes via Anodic Stripping Voltammetry,” Analytica Chimica Acta, Vol. 620, No. 1-2, July 2008, pp. 44-49. doi:10.1016/j.aca.2008.05.015

[20]   H. Y. Ma, L. P. Zhang, Y. Pan, K. Y. Zhang and Y. Z. Zhang, “A Novel Electrochemical DNA Biosensor Fabricated with Layer-by-Layer Covalent Attachment of Multiwalled Carbon Nanotubes and Gold Nanoparticles,” Electroanalysis, Vol. 20, No. 11, June 2008, pp. 1220-1226. doi:10.1002/elan.200704169

[21]   S. J. Zhang, M. L. Xu and Y. Z. Zhang, “Simultaneous Voltammetric Detection of Salsolinol and Uric Acid in the Presence of High Concentration of Ascorbic Acid with Gold Nanoparticles/Functionalized Multiwalled Carbon Nanotubes Composite Film Modified Electrode,” Electroanalysis, Vol. 21, No. 23, December 2009, pp. 2607-2610. doi:10.1002/elan.200900228

[22]   Editorial Committee of Pharmacopoeia of People’s Republicof China, “People’s Republic of China Pharmacopoeia (Part II),” Chemical Industry Press, Beijing, 2000.