Ulrike Langmann^1*, Pierre Madl², Roman Türk¹, Werner Hofmann², Georg Brunauer¹

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¹ Department of Organismic Biology, University of Salzburg, Salzburg, Austria.
² Department of Materials Research & Physics, University of Salzburg, Salzburg, Austria.
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Abstract: Lichen vegetation reacts very sensitively to a variety of air pollutants including increased nitrogen concentrations as well as to traffic exhaust in general, which makes lichens reliable monitoring organisms for atmospheric pollution. Recent environmental studies have shown that decreasing abundance of acidophytic lichen species and the increase of nitrophytic lichens can be explained by elevated levels of atmospheric nitric-compounds adsorbed onto nanoparticles. One major source of these atmospheric compounds amongst a wider pollution inventory is diesel exhaust—a mixture of gases and particle matter. This study aimed to shed light on the impact of diesel exhaust on the viability of six differently sensitive lichen species. Diesel exhaust particle concentrations in the laboratory experiments resembled those at a local highway during rush hour. By incubation in a closed stainless steel chamber we could exclude influences from other pollutants than diesel exhaust providing explicit data about the effects of diesel exhaust on lichens. The investigations revealed effects on the photosynthesis of the lichen photobionts and hence the lichen vitality. The conclusions of this study are that 1) the photobiont is affected stronger as the mycobiont and 2) older parts of the lichen are damaged first. Another remarkable result of this study is that 3) these lichens are regenerating to some extent during incubation-free periods—unless the organism is not damaged too much to restore photosynthetic activity. To our knowledge this is the first study evaluating the impact of diesel exhaust on lichens under laboratory conditions separate from other interfering pollutants.

Keywords: Lichens, Biomonitors, Diesel Exhaust, Photosynthetic Yield, Gas Exchange

Cite this paper: Langmann, U. , Madl, P. , Türk, R. , Hofmann, W. and Brunauer, G. (2014) Sensitivity of Lichens to Diesel Exhaust under Laboratory Conditions. Journal of Environmental Protection, 5, 1331-1341. doi: 10.4236/jep.2014.513127.

References

[1]   Nylander, M. (1866) Les Lichens du Jardin du Luxembourg. Bulletin de la Societe botanique de France, 13, 364-371.

[2]   De Wit, T. (1983) Lichens as Indicator for Air Quality. Environmental Monitoring and Assessment, 3, 273-282.
http://dx.doi.org/10.1007/BF00396221

[3]   Nimis, P.L., Scheidegger, C. and Wolseley, A.P. (2002) Monitoring with Lichens-Monitoring Lichens. Kluwer Academic Publishers, Dordrecht.

[4]   Conti, M.E. and Cecchetti, G. (2001) Biological Monitoring: Lichens as Bioindicators of Air Pollution Assessment—A Review. Environmental Pollution, 114, 471-492.
http://dx.doi.org/10.1016/S0269-7491(00)00224-4

[5]   Olsen, H.B., Berthelsen, K., Andersen, H.V. and Søchting, U. (2010) Xanthoria Parietina as a Monitor of Ground-Level Ambient Ammonia Concentrations. Environmental Pollution, 158, 455-461.
http://dx.doi.org/10.1016/j.envpol.2009.08.025

[6]   Munzi, S., Pisani, T., Paoli, L. and Loppi, S. (2010) Time- and Dose-Dependency of the Effects of Nitrogen Pollution on Lichens. Ecotoxicology and Environmental Safety, 73, 1785-1788. http://dx.doi.org/10.1016/j.ecoenv.2010.07.042

[7]   Sparrius, L.B. (2007) Response of Epiphytic Lichen Communities to Decreasing Ammonia Air Concentrations in a Moderately Polluted Area of the Netherlands. Environmental Pollution, 146, 375-379.
http://dx.doi.org/10.1016/j.envpol.2006.03.045

[8]   Coffey, H.M.P. and Fahrig, L. (2012) Relative Effects of Vehicle Pollution, Moisture and Colonization Sources on Urban Lichens. Journal of Applied Ecology, 49, 1467-1474.

[9]   Frahm, J.P., Janßen, A.M., Schumacher, J., Thonnes, D., Hensel, S., Heidelbach, B. and Erler, D. (2009) Das Nitrophytenproblem bei epiphytischen Flechten—Eine Synthese. Archive for Lichenology, 5, 1-8.

[10]   Bobbink, R., Hicks, K., Galloway, J., Spranger, T., Alkemade, R., Ashmore, M., Bustamante, M., Cinderby, S., Davidson, E., Dentener, F., Emmett, B., Erisman, J.W., Fenn, M., Gilliam, F., Nordin, A., Pardo, L. and De Vries, W. (2010) Global Assessment of Nitrogen Deposition Effects on Terrestrial Plant Diversity: A Synthesis. Ecological Applications, 20, 30-59.
http://dx.doi.org/10.1890/08-1140.1

[11]   Frahm, J.P. (2008) überdüngung und Versalzung durch Katalysatoren? Nitrophile Moose und Flechten nehmen zu. Biologie in unserer Zeit, 38, 94-101.
http://dx.doi.org/10.1002/biuz.200810362

[12]   Lovett, G.M., Tear, T.H., Evers, D.C., Findlay, S.E.G., Cosby, B.J., Dunscomb, J.K., Driscoll, C.T. and Weathers, K.C. (2009) Effects of Air Pollution on Ecosystems and Biological Diversity in the Eastern United States. Annals of the New York Academy of Sciences, 1162, 99-135.
http://dx.doi.org/10.1111/j.1749-6632.2009.04153.x

[13]   Gombert, S., Asta, J. and Seaward, M.R.D. (2003) Correlation between the Nitrogen Concentration of Two Epiphytic Lichens and the Traffic Density in an Urban Area. Environmental Pollution, 123, 281-290.
http://dx.doi.org/10.1016/S0269-7491(02)00367-6

[14]   Johansson, O., Nordin, A., Olofsson, J. and Palmqvist, K. (2010) Responses of Epiphytic Lichens to an Experimental Whole-Tree Nitrogen-Deposition Gradient. New Phytologist, 188, 1075-1084.
http://dx.doi.org/10.1111/j.1469-8137.2010.03426.x

[15]   Dahlman, L., Persson, J., Palmqvist, K. and Nasholm, T. (2004) Organic and Inorganic Nitrogen Uptake in Lichens. Planta, 219, 459-467.
http://dx.doi.org/10.1007/s00425-004-1247-0

[16]   Hauck, M. (2010) Ammonium and Nitrate Tolerance in Lichens. Environmental Pollution, 158, 1127-1133.
http://dx.doi.org/10.1016/j.envpol.2009.12.036

[17]   Burton, L.D. (2013) Introduction to Forestry Science. Delmar, New York.

[18]   Madl, P., Heinzelmann, E., Hofmann, W. and Türk, R. (2010) Motorway Exhaust Aerosols and Their Effects on Epiphytic Lichen Populations. Gefahrstoffe-Reinhaltung der Luft/Air Quality Control, 4, 147-153.

[19]   Madl, P. (2009) Anthropogenic Environmental Aerosols: Measurements and Biological Implication. Ph.D. Dissertation, University of Salzburg, Salzburg.

[20]   Frati, L., Santoni, S., Nicolardi, V., Gaggi, C., Brunialti, G., Guttova, A., Gaudino, S., Pati, A., Pirintsos, S. and Loppi, S. (2007) Lichen Biomonitoring of Ammonia Emission and Nitrogen Deposition around a Pig Stockfarm. Environmental Pollution, 146, 311-316.
http://dx.doi.org/10.1016/j.envpol.2006.03.029

[21]   Bowman, W.D., Gartner, J.R., Holland, K. and Wiedermann, M. (2006) Nitrogen Critical Loads for Alpine Vegetation and Terrestrial Ecosystem Response: Are We There Yet? Ecological Applications, 16, 1183-1193.
http://dx.doi.org/10.1890/1051-0761(2006)016[1183:NCLFAV]2.0.CO;2

[22]   Riddell, J., Jovan, S., Padgett, P.E. and Sweat, K. (2011) Tracking Lichen Community Composition Changes Due to Declining Air Quality over the Last Century: The Nash Legacy in Southern California. Bibliotheca Lichenologica, 106, 263-277.

[23]   Munzi, S., Pisani, T. and Loppi, S. (2009) The Integrity of Lichen Cell Membrane as a Suitable Parameter for Monitoring Biological Effects of Acute Nitrogen Pollution. Ecotoxicology and Environmental Safety, 72, 2009-2012.
http://dx.doi.org/10.1016/j.ecoenv.2009.05.005

[24]   Munzi, S., Pirintsos, A. and Loppi, S. (2009) Chlorophyll Degradation and Inhibition of Polyamine Biosynthesis in the Lichen Xanthoria Parietina under Nitrogen Stress. Ecotoxicology and Environmental Safety, 72, 281-285.
http://dx.doi.org/10.1016/j.ecoenv.2008.04.013

[25]   Kwasny, F., Madl, P. and Hofmann, W. (2009) Urban Aerosols in the City and County of Salzburg-Particle Concentration, Size Distribution and Air Quality Data. Field Campaign Commissioned for Salzburg County (German Title: Nanopartikel in Salzburg Stadt und Umland Sommerund Wintererhebung). In: Madl, P., Ed., Anthropogenic Environmental Aerosols: Measurements and Biological Implications, Ph.D. Dissertation.

[26]   Walz GmbH (1999) Photosynthesis Yield Analyzer, Mini-PAM Portable Chlorophyll Fluorometer, Handbook of Operation. Walz GembH, Effeltrich.

[27]   Walz GmbH (2009) Imaging-PAM Chlorophyll Fluorometer, Instrument Description. Walz GmbH, Effeltrich.

[28]   Gross, R.E., Pugno, P. and Dugger, W.M. (1970) Observations on the Mechanism of Copper Damage in Chlorellal. Plant Physiology, 46, 183-185.
http://dx.doi.org/10.1104/pp.46.2.183

[29]   Parkhill, J., Maillet, G. and Cullen, J.J. (2001) Fluorescence-Based Maximal Quantum Yield for PSII as a Diagnostic of Nutrient Stress. Journal of Phycology, 37, 517-529.
http://dx.doi.org/10.1046/j.1529-8817.2001.037004517.x

[30]   Willits, D.H. and Peet, M.M. (2001) Measurement of Chlorophyll Fluorescence as a Heat Stress Indicator in Tomato: Laboratory and Greenhouse Comparisons. Journal of the American Society for Horticultural Science, 126, 188-194.

[31]   Jensen, M. (1994) Assessment of Lichen Vitality by the Chlorophyll Fluorescence Parameter Fv/Fm. Cryptogamic Botany, 4, 187-192.

[32]   Jensen, M. and Kricke, R. (2002) Chlorophyll Fluorescence Measurements in the Field: Assessment of the Vitality of Large Numbers of Lichen Thalli. In: Monitoring with Lichens—Monitoring Lichens, Kluwer Academic Publishers, London, 327-332.
http://dx.doi.org/10.1007/978-94-010-0423-7_29

[33]   Mayer, W., Pfefferkorn-Dellali, V., Türk, R., Dullinger, S., Mirtl, M. and Dirnbock, T. (2013) Significant Decrease in Epiphytic Lichen Diversity in a Remote Area in the European Alps, Austria. Basic and Applied Ecology, 14, 396-403.
http://dx.doi.org/10.1016/j.baae.2013.05.006

[34]   Ellis, C.J. and Coppins, B.J. (2007) Changing Climate and Historic-Woodland Structure Interact to Control Species Diversity of the “Lobarion” Epiphyte Community in Scotland. Journal of Vegetation Science, 18, 725-734.
http://dx.doi.org/10.1111/j.1654-1103.2007.tb02587.x

[35]   Bjerke, J.W. (2009) Ice Encapsulation Protects Rather than Disturbs the Freezing Lichen. Plant Biology, 11, 227-235.
http://dx.doi.org/10.1111/j.1438-8677.2008.00113.x

[36]   Bjerke, J.W. (2011) Winter Climate Change: Ice Encapsulation at Mild Subfreezing Temperatures Kills Freeze-Tolerant Lichens. Environmental and Experimental Botany, 72, 404-408.
http://dx.doi.org/10.1016/j.envexpbot.2010.05.014

[37]   Xue, W., Li, X.Y., Zhu, J.T. and Lin, L.S. (2012) Effects of Temperature and Irradiance on Photosystem Activity during Alhagi sparsifolia Leaf Senescence. Biologia Plantarum, 56, 785-788.
http://dx.doi.org/10.1007/s10535-012-0145-8

[38]   Murata, N., Takahashi, S., Nishiyama, Y. and Allakhverdiev, S.I. (2007) Photoinhibition of Photosystem II under Environmental Stress. Biochimica et Biophysica Acta, 1767, 414-421.
http://dx.doi.org/10.1016/j.bbabio.2006.11.019

[39]   Quispel, A. (1960) Respiration of Lichens. In: Wolf, J., Ed., Pflanzenatmung Einschließlich Garung und Saurestoffwechsel (Handbuch der Pflanzenphysiologie), Springer Berlin, Heidelberg, New Yor.

[40]   Gaio-Oliveira, G., Dahlman, L., Palmqvist, K., Martins-Loucao, M.A. and Maguas, C. (2005) Nitrogen Uptake in Relation to Excess Supply and Its Effects on the Lichens Evernia prunastri (L.) Ach and Xanthoria parietina (L.) Th. Fr. Planta, 220, 794-803.
http://dx.doi.org/10.1007/s00425-004-1396-1

[41]   Meychik, N.R., Lyubimova, E.G. and Yermakov, I.P. (2010) Ion-Exchange Properties of the Cell Wall of Reindeer Lichen Cladonia rangiferina. Russian Journal of Plant Physiology, 57, 260-266.
http://dx.doi.org/10.1134/S1021443710020147

[42]   Munzi, S., Loppi, S., Cruz, C. and Branquinho, C. (2011) Do Lichens Have “Memory” of Their Native Nitrogen Environment? Planta, 233, 333-342.
http://dx.doi.org/10.1007/s00425-010-1300-0

[43]   Nixon, P.J., Michoux, F., Yu, J., Boehm, M. and Komenda, J. (2010) Recent Advances in Understanding the Assembly and Repair of Photosystem II. Annals of Botany, 106, 1-16.
http://dx.doi.org/10.1093/aob/mcq059

[44]   Keren, N. and Krieger-Liszkay, A. (2011) Photoinhibition: Molecular Mechanisms and Physiological Significance. Physiologia Plantarum, 142, 1-5.
http://dx.doi.org/10.1111/j.1399-3054.2011.01467.x

[45]   Takahashi, S. and Murata, N. (2008) How Do Environmental Stresses Accelerate Photoinhibition? Trends in Plant Science, 13, 178-182.
http://dx.doi.org/10.1016/j.tplants.2008.01.005

[46]   Riddell, J., Nash, T.H. and Padgett, P. (2008) The Effect of HNO3 Gas on the Lichen Ramalina menziesii. Flora-Morphology, Distribution, Functional Ecology of Plants, 203, 47-54.
http://dx.doi.org/10.1016/j.flora.2007.10.001

[47]   Riddell, J., Padgett, P.E. and Nash, T.H. (2012) Physiological Responses of Lichens to Factorial Fumigations with Nitric Acid and Ozone. Environmental Pollution, 170, 202-210.
http://dx.doi.org/10.1016/j.envpol.2012.06.014