JEP  Vol.5 No.13 , October 2014
Dioxins in the Food Chain: Contamination Fingerprint Analysis in Breeding Hens, Hatching Eggs and Broilers
Abstract: While routine monitoring poultry meat was obtained from breeding hens, dioxins contaminations were detected in Portugal. Levels of 430.9 pg PCDD/F-WHO-TEQ/g1 were found, which are higher than the official limits legally allowed for this matrix (1.75 pg PCDD/F-WHO-TEQ/g). To identify the magnitude of the contaminations, 60 samples were collected from poultry farms and different matrices, namely: feed, water, wood shavings from the litters, muscle of the breeding hens, hatching eggs collected in the positive farm and muscle collected from broilers farms supplied by the positive breeding farm. The comparison of the dioxins congeners profiles showed that there was a coincidence of peaks of higher relative concentrations in the wood shavings, with the peaks of the highest relative concentration in the hatching eggs, especially the relative concentrations of the congeners 1,2,3,4,6,7,8-HpCDD and OCDD, which may be explained by the role of VLDLy in the delivery of triglycerides to the oocyte, where they will be used as the energy source for the developing embryo. The comparison of the dioxins congeners profiles of the breeding hens muscle with the poultry muscle, showed a coincidence of peaks of higher relative concentrations in the congeners 1,2,3,7,8-PeCDD, 1,2,3,6,7,8-HxCDD, 1,2,3,4,6,7,8-HpCDD and OCDD which may indicate a dechlorination pathway “in vivo”. Results allowed concluding that those wood shavings, improperly used as poultry litters, were certainly the source of contamination of the food chain.
Cite this paper: Cardo, M. , Castel-Branco, M. , Andreozzi, V. and Bernardo, F. (2014) Dioxins in the Food Chain: Contamination Fingerprint Analysis in Breeding Hens, Hatching Eggs and Broilers. Journal of Environmental Protection, 5, 1323-1330. doi: 10.4236/jep.2014.513126.

[1]   De Vries, M., Kwakkel, R. and Kijlstra, A. (2006) Dioxins in Organic Eggs: A Review. Wageningen Journal of Life Sciences, 54, 207-221.

[2]   Kennedy, J., Delaney, L., McGloin, A., Hudson, E. and Wall, P. (2010) Public Perceptions of the Dioxin Crisis in Irish Pork.

[3]   Cardo, M., Félix, V. and Bernardo, F. (2009) Dioxins in Poultry Products: A Case Study. Journal of Veterinary Pharmacology and Therapeutics, 32, 95.

[4]   United States Environment Protection Agency (1994) Method 1613 Revision B-Tetra—Through Octa-Chlorinated Dioxins and Furans by Isotope Dilution HRGC/HRMS. United States Environment Protection Agency, Washington DC, 89.

[5]   Russell, H. and Plumb, J. (2004) Fingerprint Analysis of Contaminant Data: A Forensic Tool for Evaluating Environmental Contamination. National Exposure Research Laboratory Environmental Sciences Division, Las Vegas, 27.

[6]   Johnson, R. and Wichern, D. (2001) Applied Multivariate Statistical Analysis. Englewood Cliffs, New Jersey. 5th Edition, Prentice Hall, Upper Saddle River, 761.

[7]   Hagenmaier, H. and Brunner, H. (1987) Isomer Specific Analysis of Pentachlorophenol and Sodium Pentachlorophenate for 2,3,7,8-Substituted PCDD and PCDF at Sub-PPB Levels. Chemosphere, 16, 1759-1764.

[8]   Fries, G.F., Feil, V. and Davison, K. (1996) The Significance of Pentachlorophenol-Treated Wood as a Source of Dioxin Residues in United States Beef. Organohalogen Compounds, 28, 156-159.

[9]   Cleverly, D., Schaum, J., Schweer, G., Becker, J. and Winters, D. (1997) The Congener Profiles of Anthropogenic Sources of Chlorinated Dibenzo-p-Dioxins and Chlorinated Dibenzofurans in the United States. The 17th International Symposium on Chlorinated Dioxins and Related Compounds, Indianapolis, 25-29 August 1997, 430-435.

[10]   Salvantem, K., Lin, G., Rosemary L., Walzem, R. and Williams, T. (2007) Characterization of Very-Low Density Lipoprotein Particle Diameter Dynamics in Relation to Egg Production in a Passerine Bird. Journal of Experimental Biology, 210, 1064-1074.

[11]   Lynam, M., Kuty, M., Damborsky, J., Koca, J. and Adriaens, P. (1998) Molecular Orbital Calculations to Describe Microbial Reductive Dechlorination of Polychlorinated Dioxins. Environmental Toxicology and Chemistry, 17, 988-997.

[12]   Huwe, J., Feil, V., Zaylskie, R. and Tiernan, T. (2000) An Investigation of the in Vivo Formation of Octachlorodibenzo-p-Dioxin. Chemosphere, 40, 957-962.

[13]   Pirard, C. and Pauw, E. (2004) Uptake of Polychlorodibenzo-p-Dioxins, Polychloro-Dibenzofurans and Coplanar Polychloro-Biphenyls in Chickens. University of Liège, Liège, Belgium. Environment International, 31, 585-591.

[14]   Petreas, M., Goldman, L., Hayward, D., Chang, R., Flattery, J., Wiesmüller, T. and Stephens, R. (1991) Biotransfer and Bioaccumulation of PCDD/PCDFs from Soil: Controlled Exposure Studies of Chickens. Chemosphere, 23, 1731-1741.