AS  Vol.5 No.12 , October 2014
Impact of Methyl Jasmonate on Antioxidant Activity and Some Biochemical Aspects of Maize Plant Grown under Water Stress Condition
ABSTRACT
A pot-culture study was undertaken to investigate the possible role of Methyl jasmonate (MeJA)-treatment on the earlier vegetative growth stage and different chemical constituents of maize cultivar (Giza-2) plants subjected to water stress. The grains were divided into two groups: first group was pre-soaked in water, and the second one was pre-soaked in 50 μM MeJA for 8 h. The plants were subjected to different levels of water field capacity (WFC) 65%, 55% and 45%. The results showed that pre-soaking maize grains with MeJA led to increases in plant growth criteria evident in terms of plant height, fresh and dry weight of plant. The pigment levels concomitantly with total carbohydrates, total soluble sugar, polysaccharides, as well as free amino acids, proline and total protein content were markedly increased. Moreover, the application of the investigated MeJA significantly improved growth hormone in terms of IAA. In contrast ABA level was markedly declined in maize plant. The activities of oxidative CAT, POX and SOD were also increased with MeJA. In addition, the N, P and K content was increased significantly in shoot. As a conclusion, soaking maize grains with MeJA could alleviate the harmful effects of water stress.

Cite this paper
Abdelgawad, Z. , Khalafaallah, A. and Abdallah, M. (2014) Impact of Methyl Jasmonate on Antioxidant Activity and Some Biochemical Aspects of Maize Plant Grown under Water Stress Condition. Agricultural Sciences, 5, 1077-1088. doi: 10.4236/as.2014.512117.
References
[1]   Heinigre, R.W. (2000) Irrigation and drought management. Crop Science Department.
http://www.ces.ncsu.edu/plymouth/cropsci/cornguide/Chapter4.html

[2]   Zhu, J.K. (2002) Salt and Drought Stress Signal Transduction in Plants. Annual Review of Plant Biology, 53, 247-258.
http://dx.doi.org/10.1146/annurev.arplant.53.091401.143329

[3]   Gulen, H. and Eris, A. (2004) Effect of Heat Stress on Peroxidase Activity And total Protein Content in Strawberry Plants. Plant Science, 166, 739-744.
http://dx.doi.org/10.1016/j.plantsci.2003.11.014

[4]   El-Tayeb, M.A. (2006) Differential Response of Two Vicia faba Cultivars to Drought: Growth, Pigments, Lipid Peroxidation, Organic Solutes, Catalase and Peroxidase Activity. Acta Agronomica Hungarica, 54, 25-37.
http://dx.doi.org/10.1556/AAgr.54.2006.1.3

[5]   Chaves, M.M., Maroco, J.P. and Pereira, J.S. (2003) Understanding Plant Responses to Drought from Genes to the Whole Plant. Functional Plant Biology, 30, 239-264.
http://dx.doi.org/10.1071/FP02076

[6]   Ramanjulu, S. and Bartels, D. (2002) Drought and Desiccation-Induced Modulation of Gene Expression in Plants. Plant, Cell and Environment, 25, 141-151.
http://dx.doi.org/10.1046/j.0016-8025.2001.00764.x

[7]   Niyogi, K.K. (1999) Photoprotection Revisited: Genetic and Molecular Approaches. Annual Review of Plant Physiology and Plant Molecular Biology, 50, 333-359.
http://dx.doi.org/10.1146/annurev.arplant.50.1.333

[8]   Avanci, N.C., Luche, D.D., Goldman, G.H. and Goldman, M.H.S. (2010) Jasmonates Are Phytohormones with Multiple Functions, Including Plant Defense and Reproduction. Genetics and Molecular Research, 9, 484-505.

[9]   Ueda, J. and Saniewski, J. (2006) Methyl Jasmonate-Induced Stimulation of Chlorophyll Formation in the Basal Part of Tulip Bulbs Kept under Natural Light Conditions. Journal of Fruit and Ornamental Plant Research, 14, 199-210.

[10]   Norastehnia, A., Sajedi, R.H. and Nojavan-Asghari, M. (2007) Inhibitory Effects of Methyl Jasmonate on Seed Germination in Maize (Zea Mays L.): Effect on Amylase Activity and Ethylene Production. General and Applied Plant Physiology, 33, 13-23.

[11]   Anjum, S.A., Wang, L.C., Farooq, M., Hussain, M., Xue, L.L. and Zou, C.M. (2011) Brassinolide Application Improves the Drought Tolerance in Maize through Modulation of Enzymatic Antioxidants and Leaf Gas Exchange. Journal of Agronomy and Crop Science, 197, 177-185.
http://dx.doi.org/10.1111/j.1439-037X.2010.00459.x

[12]   Walia, H., Wilson, C., Condamine, P., Liu, X., Ismail, A.M. and Close, T.J. (2007) Large-Scale Expression Profiling and Physiological Characterization of Jasmonic Acid-Mediated Adaptation of Barley to Salinity Stress. Plant, Cell & Environment, 30, 410-421.
http://dx.doi.org/10.1111/j.1365-3040.2006.01628.x

[13]   Lee, T.M., Lur, H.S., Lin, V.H. and Chu, C. (1996) Physiological and Biochemical Changes Related to Methyl Jasmonate-Induced Chilling Tolerance of Rice (Oryza sativa L.) Seedlings. Plant, Cell & Environment, 19, 65-74.
http://dx.doi.org/10.1111/j.1365-3040.1996.tb00227.x

[14]   Ding, C.K., Wang, C.Y., Gross, K.C. and Smith, D.L. (2002) Jasmonate and Salicylate Induce the Expression of Pathogenesis-Related-Proteins Genes and Increase Resistance to Chilling Injury in Tomato Fruit. Planta, 214, 895-901.
http://dx.doi.org/10.1007/s00425-001-0698-9

[15]   Ketabchi, S. and Shahrtash, M. (2011) Effects of Methyl Jasmonate and Cytokinin on Biochemical Responses of Maize Seedlings Infected by Fusarium moniliforme. Asian Journal of Experimental Biological Sciences, 2, 299-305.

[16]   Chen, H., Jones, A.D. and Howe, G.A. (2006) Constitutive Activation of the Jasmonate Signaling Pathway Enhances the Production of Secondary Metabolites in Tomato. FEBS Letters, 580, 2540-2546.
http://dx.doi.org/10.1016/j.febslet.2006.03.070

[17]   Browse, J. (2009) Jasmonate Passes Muster: A Receptor and Targets for the Defense Hormone. Annual Review of Plant Biology, 60, 183-205.
http://dx.doi.org/10.1146/annurev.arplant.043008.092007

[18]   Lichtenthaler, H.K. and Buschmann, C. (2001) Chlorophylls and Carotenoids: Measurement and Characterization by UV-VIS Spectroscopy. In: Wrolstad, R.E., Acree, T.E., An, H., Decker, E.A., Penner, M.H., Reid, D.S., Schwartz, S.J., Shoemaker, C.F. and Sporns, P., Eds., Current Protocols in Food Analytical Chemistry (CPFA), John Wiley and Sons, New York, F4.3.1-F4.3.8.

[19]   Homme, P.M., Gonzalez, B. and Billard, J. (1992) Carbohydrate Content, Frutane and Sucrose Enzyme Activities in Roots, Stubble and Leaves of Rye Grass (Lolium perenne L.) as Affected by Source/Link Modification after Cutting. Journal of Plant Physiology, 140, 282-291.
http://dx.doi.org/10.1016/S0176-1617(11)81080-1

[20]   Yemm, E.W. and Willis, A.J. (1954) The Respiration of Barley Plants. IX. The Metabolism of Roots during the Assimilation of Nitrogen. New Phytologist, 55, 229-234.
http://dx.doi.org/10.1111/j.1469-8137.1956.tb05283.x

[21]   Herbert, D., Phipps, P.J. and Strange, R.E. (1971) Chemical Analysis of Microbial Cells. Methods in Microbiology, 5B, 209-344.
http://dx.doi.org/10.1016/S0580-9517(08)70641-X

[22]   Smith, F., Gilles, M.A., Hamilton, J.K. and Godees, P.A. (1956) Colorimetric Method for Determination of Sugar Related Substances. Analytical Chemistry, 28, 350.
http://dx.doi.org/10.1021/ac60111a017

[23]   Bates, L.S., Waldan, R.P. and Teare, L.D. (1973) Rapid Determination of Free Proline under Water Stress Studies. Plant and Soil, 39, 205-207.
http://dx.doi.org/10.1007/BF00018060

[24]   Vartanain, N., Hervochon, P. Marcolte, L. and Larher, F. (1992) Proline Accumulation during Drought Rhizogenesis in Brassica napus var. oleifera. Journal of Plant Physiology, 140, 623-628.
http://dx.doi.org/10.1016/S0176-1617(11)80799-6

[25]   Yemm, E.W. and Cocking, E.C. (1955) The Determination of Amino Acids with Ninhydrin. Analyst, 80, 209-213.
http://dx.doi.org/10.1039/an9558000209

[26]   Bradford, M.M. (1976) A rapid and Sensitive Method for Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye-Binding. Analytical Biochemistry, 72, 248-254.
http://dx.doi.org/10.1016/0003-2697(76)90527-3

[27]   Wasfy, W.S. and Orrin, E.S. (1975) Identification of Plant Hormones from Cotton Ovules. Plant Physiology, 55, 550-554.

[28]   Shindy, W.W. and Smith, O. (1975) Identification of Plant Hormones from Cotton Ovules. Plant Physiology, 55, 550-554.
http://dx.doi.org/10.1104/pp.55.3.550

[29]   Chen, J.X. and Wang, X.F. (2006) Plant Physiology Experimental Guide. Higher Education Press, Beijing, 24-25, 55-56.

[30]   Kumar, K.B. and Khan, P.A. (1982) Peroxidase and Polyphenol Oxidase in Excised Ragi (Eleusine coracana cv. PR 202) Leaves during Senescence. Indian Journal of Experimental Botany, 20, 412-416.

[31]   Giannopolitis, C.N. and Ries, S.K. (1977) Purification and Quantitative Relationship with Water-Soluble Protein in Seedlings. Plant Physiology, 59, 315-318.

[32]   Chapman, H.O. and Pratt, P.E. (1978) Methods of Analysis for Soils, Plants and Water. Division of Agriculture Sciences University California, Berkley, 5-6.

[33]   Snedecor, G.M. and Cochran, W.C. (1969) Statistical Methods. 6th Edition, Iowa University Press. Ames, Iowa.

[34]   Creelman, R.A. and Mullet, J.E. (1997) Oligosaccharins, Brassinolides, and Jasmonates: Nontraditional Regulators of Plant Growth, Development, and Gene Expression. The Plant Cell, 9, 1211-1223.
http://dx.doi.org/10.1105/tpc.9.7.1211

[35]   Kusaka, M., Lalusin, A.G. and Fujimura, T. (2005) The Maintenance of Growth and Turgor in Pearl Millet (Pennisetum glaucum (L.) Leeke) Cultivars with Different Root Structures and Osmo-Regulation under Drought Stress. Plant Science, 168, 1-14.
http://dx.doi.org/10.1016/j.plantsci.2004.06.021

[36]   Ripley, B.S., Gilber, M.E., Ibrahim, D.G. and Osborne, C.P. (2007) Drought Constraints on C4 Photosynthesis: Stomatal and Metabolic Limitations in C3 and C4 Subspecies of Alloteropsis semialata. Journal of Experimental Botany, 58, 1351-1363.
http://dx.doi.org/10.1093/jxb/erl302

[37]   Sheng, M., Tang, M., Chan, H., Yang, B., Zhang, F. and Huang, Y. (2008) Influence of Arbuscular Mycorrhizae on Photosynthesis and Water Status of Maize Plants under Salt Stress. Mycorrhiza, 18, 287-296.
http://dx.doi.org/10.1007/s00572-008-0180-7

[38]   Mittler, R. (2002) Oxidative Stress, Antioxidants, and Stress Tolerance. Trends in Plant Science, 7, 405-410.
http://dx.doi.org/10.1016/S1360-1385(02)02312-9

[39]   Murkute, A.A., Sharma, S. and Singh, S.K. (2006) Studies on Salt Stress Tolerance of Citrus Rootstock Genotypes with Arbuscular Mycorrhizal Fungi. Horticultural Science, 33, 70-76.

[40]   EL-Bassiouny, H.M.S. (1997) Studies on the Role of Abscisic as Antitranspirant on Growth, Chemical Analysis and Yield Components of Cowpea Plants in Presence of Soil Conditioners Egypt. The Journal of Physiological Sciences, 3, 409-432.

[41]   Fischer, C. and Holl, W. (1991) Food Reserves in Scots Pine (Pinus sylvestris L.). I. Seasonal Changes in the Carbohydrate and Fat Reserves of Pine Needles. Trees, 5, 187-195.
http://dx.doi.org/10.1007/BF00227524

[42]   Patakas, A. and Noitsakis, B. (2001) Leaf Age Effects on Solute Accumulation in Water-Stressed Grapevines. Plant Physiology, 158, 63-69.
http://dx.doi.org/10.1078/0176-1617-00003

[43]   Hoekstra, F.A. and Buitink, J. (2001) Mechanisms of Plant Desiccation Tolerance. Trends in Plant Science, 8, 431-438.
http://dx.doi.org/10.1016/S1360-1385(01)02052-0

[44]   Khattab, H. (2007) Role of Glutathione and Polyadenylic Acid on the Oxidative Defense Systems of Two Different Cultivars of Canola Seedlings Grown under Saline Conditions. Australian Journal of Basic and Applied Sciences, 1, 323-334.

[45]   Sadak, M.Sh., Abdelhamid, T.M. and El-Saady, A.M. (2010) Physiological Responses of Faba Bean Plant to Ascorbic Acid Grown under Salinity Stress. Egyptian Journal of Agronomy, under press.

[46]   Gzik, A. (1996) Accumulation of Proline and Pattern of α-Amino Acids in Sugar Beet Plants in Response to Osmotic, Water and Salt Stress. Environmental and Experimental Botany, 36, 29-38.
http://dx.doi.org/10.1016/0098-8472(95)00046-1

[47]   Bandurska, H. (1993) In Vivo and in Vitro Effect of Proline on Nitrate Reductase Activity under Osmotic Stress in Barley. Acta Physiologiae Plantarum, 15, 83-88.

[48]   Rajaravindran, M. and Natarajan, S. (2012) Effects of Salinity Stress on Growth and Biochemical Constituents of the Halophyte Sesuvium portulacastrum. International Journal of Research in Biological Sciences, 2, 18-25.

[49]   Krasensky, J. and Jonak, C. (2013) Drought, Salt, and Temperature Stress-Induced Metabolic Rearrangements and Regulatory Networks. Journal of Experimental Botany, 4, 1593-1608.

[50]   Harinasut, P., Srisunak, S., Pitukchaisopol, S. and Charoensataporn, R. (2000) Mechanisms of Adaptations to Increasing Salinity of Mulberry: Proline Content and Ascorbate Peroxidase Activity in Leaves of Multiple Shoots. ScienceAsia, 26, 207-211.
http://dx.doi.org/10.2306/scienceasia1513-1874.2000.26.207

[51]   Hoque, M.A., Okuma, E., Banu, M.N.A., Nakamura, Y., Shimoishi, Y. and Murata, Y. (2007) Exogenous Proline Mitigates the Detrimental Effects of Salt Stress More than Exogenous Betaine by Increasing Antioxidant Enzyme Activities. Journal of Plant Physiology, 164, 553-561.

[52]   Rohwer, C.L. and Erwin J.E. (2008) Horticultural Applications of Jasmonates: A Review. The Journal of Horticultural Science and Biotechnology, 83, 283-304.

[53]   Mahmood, M., Bidabadi, S.S., Ghobadi, C. and Gray, D.J. (2012) Effect of Methyl Jasmonate Treatments on Alleviation of Polyethylene Glycol-Mediated Water Stress in Banana (Musa acuminata cv. “Berangan”, AAA) Shoot Tip Cultures. Plant Growth Regulation, 68, 161-169.
http://dx.doi.org/10.1007/s10725-012-9702-6

[54]   Shakirova, F.M., Sakhabutdinova, A.R., Bezrukova, M.V., Falkhutdinova, R.A. and Fatkhutdinova, D.R. (2003) Changes in the Hormonal Status of Wheat Seedlings Induced by Salicylic Acid and Salinity. Plant Science, 164, 317-322.
http://dx.doi.org/10.1016/S0168-9452(02)00415-6

[55]   Sakhabutdinova, A.R., Fatkhutdinova, D.R., Bezrukova, M.V. and Shakirova, F.M. (2004) Salicylic Acid Prevents the Damaging Action of Stress Factors on Wheat Plant Bulg. Bulgarian Journal of Plant Physiology, Special Issue 2003, 314-319.

[56]   Setter, T.L., Yan, J., Warburton, M., Ribaut, J.M., Xu, Y., Sawkins, M., Buckler, E.S., Zhang, Z. and Gore, M.A. (2011) Genetic Association Mapping Identifies Single Nucleotide Polymorphisms in Genes That Affect Abscisic Acid Levels in Maize Floral Tissues during Drought. Journal of Experimental Botany, 62, 701-716.
http://dx.doi.org/10.1093/jxb/erq308

[57]   Gonzalez, E.M., Galvez, L. and Arrese-Igor, C. (2001) Abscisic Acid Induces a Decline in Nitrogen Fixation That Involves Leghemoglobin, but Is Independent of Sucrose Synthase Activity. Journal of Experimental Botany, 52, 285-293.
http://dx.doi.org/10.1093/jexbot/52.355.285

[58]   Majid, N.A. and Akbar, N. (2006) A Possible Role for Methyl Jasmonate in Affecting Superoxide Dismutase and Catalase Activities under PQ-Induced Oxidative Stress in Maize Seedlings. Journal of Biological Sciences, 6, 55-60.
http://dx.doi.org/10.3923/jbs.2006.55.60

[59]   Norastehnia, A. and Nojavan-Asghari, M. (2006) Effect of Methyl Jasmonate on the Enzymatic Antioxidant Defense System in Maize Seedling Subjected to Paraquat. Asian Journal of Plant Sciences, 5, 17-23.
http://dx.doi.org/10.3923/ajps.2006.17.23

[60]   Keramat, B., Kalantari, K.M. and Arvin, M.J. (2009) Effects of Methyl Jasmonate in Regulating Cadmium Induced Oxidative stress in Soybean Plant (Glycine max L.). African Journal of Biotechnology, 3, 240-244.

[61]   Choudhury, S. and Panda, S.K. (2004) Role of Salicylic Acid in Regulating Cadmium Induced Oxidative Stress in Oryza sativa L. Roots. Bulgarian Journal of Plant Physiology, 30, 95-110.

[62]   Li, L., Staden, J.V. and Jager, A.K. (1998) Effect of Plant Growth Regulators on the Antioxidant System in Seedlings of Two Maize Cultivars Subjected to Water Stress. Journal of Plant Growth Regulation, 25, 81-87.
http://dx.doi.org/10.1023/A:1010774725695

[63]   Jung, S. (2004) Effect of Chlorophyll Reduction in Arabidopsis thaliana by Methyl Jasmonate or Norflurazon on Antioxidant Systems. Plant Physiology and Biochemistry, 42, 231-255.
http://dx.doi.org/10.1016/j.plaphy.2004.01.001

[64]   Tariq, A., Masroor, M., Khan, A., Mohd, I., Naeem, M., Moinuddin, I. and Nadeem, H. (2011) Methyl Jasmonate Counteracts Boron Toxicity by Preventing Oxidative Stress and Regulating Antioxidant Enzyme Activities and Artemisinin Biosynthesis in Artemisia annua L. Protoplasma, 248, 601-612.

[65]   Ruiz-Lozano, J.M., Azcón, R. and Gómez, M. (1995) Effects of Arbuscular-Mycorrhizal Glomus Species on Drought Tolerance: Physiological and Nutritional Plant Responses. Applied and Environmental Microbiology, 61, 456-460.

[66]   Morte, A., Lovisolo, C. and Schubert, A. (2000) Effect of Drought Stress on Growth and Water Relations of the Mycorrhizal Association Helianthemum almeriense-Terfezia claveryi. Mycorrhiza, 10, 115-119.
http://dx.doi.org/10.1007/s005720000066

[67]   Baque, M.A., Karim, M.A., Hamid, A. and Tetsushi, H. (2006) Effect of Fertilizer Potassium on Growth, Yield and Nutrient Uptake of Wheat (Triticum aestivum L.) under Water Stress Conditions. South Pacific Studies, 27, 25-35.

[68]   Maria, A.M., Gendy, A.A., Selim, A.H. and Abd El.-All, A.M. (2008) Response of Wheat Plants Grown under Water Stress in Relation to Jasmonic Acid. Minufiya Journal of Agricultural Research, 33, 1355-1375.

[69]   Hammad, S.A.R. and Ali, O.A.M. (2014) Physiological and Biochemical Studies on Drought Tolerance of Wheat Plants by Application of Amino Acids and Yeast Extract. Annals of Agricultural Sciences, 59, 133-145.
http://dx.doi.org/10.1016/j.aoas.2014.06.018

 
 
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