Xinzhi Cao, Yingqing Zhao, Li Xiong, Linsheng Ren, Jia Liu

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Department of Bioengineering, Sichuan University of Science and Engineering, Zigong, China.
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Abstract: Rotational speed, temperature, inoculation amount and pH are the four factors that affect the submerge fermentation of Agaricus blazei Murill. The orthogonal experiment was on the basis of single factor experiments. And the optimal submerge fermentation condition of Agaricus blazei Murill was determined through orthogonal test. The results were as follows: temperature (25℃), rotational speed (150 r/min), inoculation amount (6%) and pH (7), in this condition the cell dry weight reached 1.32 g/100mL; temperature (25℃), rotational speed (150 r/min), inoculation amount (8%) and pH (6.5) in this condition the extracellular polysaccharide reached 6.95 mg/mL.

Keywords: Agaricus blazei Murill, Submerged Fermentation, Cell Dry Weight, Extracellular Polysaccharide

Cite this paper: Cao, X. , Zhao, Y. , Xiong, L. , Ren, L. and Liu, J. (2014) Research of Submerge Fermentation Conditions on Agaricus blazei Murill. Food and Nutrition Sciences, 5, 1926-1943. doi: 10.4236/fns.2014.519204.

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