ABB  Vol.2 No.3 , June 2011
Genetic diversity among Indian phytopathogenic isolates of Fusarium semitectum Berkeley and Ravenel
Abstract: We report total ten isolates of F. semitectum recovered from different hosts. Identity of these isolates was determined by morphological and cultural characteristics and confirmed by RAPD-PCR analysis using forty random primers. Morphologically all the ten isolates showed similarity, but based on RAPD-PCR analysis, these isolates can be categorized in three groups depending upon similarity co-efficient. Genetic similarity coefficients between pair wise isolates varied from 0.00 to 1.95 based on an unweighted paired group method of arithmetic average (UPGMA) cluster analysis. RAPD-PCR technique can be used as an important tool for the genetic differentiation among isolates of F. semitectum.
Cite this paper: nullIngle, A. and Rai, M. (2011) Genetic diversity among Indian phytopathogenic isolates of Fusarium semitectum Berkeley and Ravenel. Advances in Bioscience and Biotechnology, 2, 142-148. doi: 10.4236/abb.2011.23023.

[1]   Ingle, A., Karwa, A., Rai, M.K. and Gherbawy, Y. (2009) Fusarium: molecular detection, mycotoxins and biocontrol. In: Gherbawy, Y., Mach, R. and Rai, M. Eds., Current Advances in Molecular Mycology, Science Publishers Inc., Enfield, 85-106.

[2]   Steinkellner, S., Mammerler R. and Vierheilig, H. (2008) Germination of Fusarium oxysporum in root exudates from tomato plants challenged with different Fusarium oxysporum strains. European Journal Plant Pathology, 122, 395-401. doi:10.1007/s10658-008-9306-1

[3]   Godoy, P., et al. (2004) Onychomycosis caused by Fusarium solani and Fusarium oxysporum in S?o Paulo, Brazil. Mycopathologia, 157, 287-290. doi:10.1023/B:MYCO.0000024186.32367.d4

[4]   Mansoory, D., Roozbahany, N.A., Mazinany, H. and Samimagam, A. (2003) Chronic Fusarium infection in an adult patient with undiagnosed chronic granulomatous disease. Clinical Infectious Diseases, 37, 107-108. doi:10.1086/377608

[5]   Nucci, M. and Anaissie, E. (2007) Fusarium infections in immunocompromised patients. Clinical Microbiology Review, 20, 695-704. doi:10.1128/CMR.00014-07

[6]   Sultana, N. and Hanif, N.Q.N. (2009) Mycotoxin contamination in cattle feed and feed ingredients. Pakistan Veterinary Journal, 29, 211-213.

[7]   Girish, G.K. and Goyal, R.K. (1986) Prevention and control of mycotoxins in food grains in India. Bull Grain Technology, 24, 157-177.

[8]   Knight, C., Cutts, D.F. and Colhoun, J. (2008) The role of Fusarium semitectum in causing crown rot of banana. Journal Phytopathology, 89, 170-176. doi:10.1111/j.1439-0434.1977.tb02853.x

[9]   Hawa, M.M., Salleh, B. and Latiffah, Z. (2010) Characterization and intraspecific variation of Fusarium semitectum (Berkeley and Ravenel) associated with red-fleshed dragon fruit (Hylocereus polyrhizus [Weber] Britton and Rose) in Malaysia. African Journal Biotechnology, 9, 273-284.

[10]   Mikunthan, G. and Manjunatha, M. (2006) Fusarium se- mitectum, a potential mycopathogen against thrips and mites in chilli, Capsicum annuum. Communications in Agricultural and Applied Biological Sciences, 71, 449-463.

[11]   Manjunatha, A.M., et al. (2009) Evaluation of fungal pathogen, Fusarium semitectum Berk and Ravenel against tobacco aphid under laboratory and greenhouse conditions. Karnataka Journal of Agriculture Science, 22, 495-498.

[12]   Seifert, K. (1996) Fusarium interactive key. Agriculture and Agri food Canada, 1-30.

[13]   Leslie, J.F. and Summerell, B.A. (2006) The Fusarium laboratory manual. 3rd Ed., Blackwell Publishing Professional, Ames.

[14]   Gupta, V.K., et al. (2009) Studies of genetic polymorphism in the isolates of Fusarium solani. Australian Journal Crop Science, 3, 101-106.

[15]   Niessen, L. (2007) PCR based diagnosis and quantification of mycotoxin producing fungi. International Journal Food Microbiology, 119, 38-46. doi:10.1016/j.ijfoodmicro.2007.07.023

[16]   Feng, J.I.E., et al. (2000) RAPD analysis of physiologic races of Fusarium oxysporum f. sp. vasinfectum in China. Mycosystema, 19, 45-50.

[17]   Smith, S.N., et al. (2001) Soil-borne populations of Fusarium oxysporum f. sp. vasinfectum, a cotton wilt fungus in California fields. Mycologia, 93, 737-743. doi:10.2307/3761828

[18]   Nagarajan, G., et al. (2004) Genetic variation in Fusarium oxysporum f. sp. fragariae populations based on RAPD and rDNA RFLP analyses. Plant Pathology Journal, 20, 264-270. doi:10.5423/PPJ.2004.20.4.264

[19]   Nelson, P.E., Toussoun, T.A and Marasas, W.F.O. (1983) Fusarium species: an illustrated manual for identification. Pennsylvania State University Press, University Park.

[20]   Sutton, D.A., Fothergill, A.W. and Rinaldi, M.G. (1998) Guide to clinically significant fungi, 1st Ed., Williams & Wilkins, Baltimore.

[21]   Abd-Elsalam, K.A., Schnieder, F., et al. (2003) Intra- -species genomic groups in Fusarium semitectum and their correlation with origin and cultural characteristics. Journal Plant Disease Protection, 110, 409-418.

[22]   Assigbetse, K.B., et al. (1994) Differentiation of Fusarium oxysporum f. sp. vasinfectum races on cotton by Random amplified polymorphic DNA (RAPD) analysis. Phytopathology, 84, 622-626. doi:10.1094/Phyto-84-622

[23]   Edel, V., et al. (2001) Genetic diversity of Fusarium oxysporum populations isolated from different soils in France. FEMS Microbiology and Ecology, 36, 61-71. doi:10.1111/j.1574-6941.2001.tb00826.x

[24]   Leslie, J.F., et al. (2007) Inter- and intra specific genetic variation in Fusarium. International Journal Food Microbiology, 119, 25-32. doi:10.1016/j.ijfoodmicro.2007.07.059

[25]   El-Fadly, G.B., et al. (2008) Identification of some Fusarium spp. using RAPD-PCR technique. Egyptian Journal Phytopathology, 36, 71-80.