AiM  Vol.4 No.13 , October 2014
A New Epidemiological Tool for Staphylococcus aureus Surveillance
ABSTRACT
Epidemiological surveillance for microbes is currently based on either agar culture followed by identification, or genetic amplification. Both techniques are highly skilled-labor intensive, costly, and must be done in central laboratories. The Defined Substrate Utilization® (DSU®) format provides an epidemiological series of specific screening formulations that obviate these limitations. All reagents are present in optimized stable powder form in a test tube—add water, inoculate, and incubate. A specific color change provides a sensitive and specific detection of the target microbe. Two DSU® methods for Staphylococcus aureus (S. aureus) are presented: aureusAlert® for all S. aureus and EPI-M® for methicillin-resistant S. aureus (MRSA). Both aureusAlert® and EPI-M® had a detection level of 20 colony forming units (CFU) in 18 hours. aureusAlert® and conventional methods agreed 93.6% and EPI-M® and conventional methods agreed 94.1%. DSU® and conventional methods showed the same specificity.

Cite this paper
Edberg, S. and Miller, J. (2014) A New Epidemiological Tool for Staphylococcus aureus Surveillance. Advances in Microbiology, 4, 919-924. doi: 10.4236/aim.2014.413102.
References
[1]   Health Protection Agency (2012) Investigation of Specimens for Screening for MRSA. UK Standards for Microbiology Investigations, B 29 Issue 5.2.

[2]   Flayhart, D., Hindler, J.F., Bruckner, D.A., Hall, G., Shrestha, R.K., Vogel, S.A., Richter, S.S., Howard, W., Walther, R. and Carroll, K.C. (2005) Multicenter Evaluation of BBL CHROMagar MRSA Medium for Direct Detection of Methicillin-Resistant Staphylococcus aureus from Surveillance Cultures of the Anterior Nares. Journal of Clinical Microbiology, 43, 5536-5540.
http://dx.doi.org/10.1128/JCM.43.11.5536-5540.2005

[3]   Kluytmans, J., van Belkum, A. and Verbrugh, H. (1997) Nasal Carriage of Staphylococcus aureus: Epidemiology, Underlying Mechanisms, and Associated Risks. Clinical Microbiology Reviews, 10, 505-520.

[4]   Biorad, MRSA SelectTM (2013) Combined Nasal and Wound Specimens, Package Insert.

[5]   Yang, S. and Rothman, R.E. (2004) PCR-Based Diagnostics for Infectious Diseases: Uses, Limitations, and Future Applications in Acute-Care Settings. The Lancet Infectious Diseases, 4, 337-348. http://dx.doi.org/10.1016/S1473-3099(04)01044-8

[6]   Garcia-Alvarez, L., Holden, M.T.G., Lindsay, H., Webb, C.R., Brown, D.F.J., Curran, M.D., Walpole, E., Brooks, K., Pickard, D.J., Teale, C., Parkhill, J., Bentley, S.D., Edwards, G.F., Girvan, E.K., Kearns, A.M., Pichon, B., Hill, R.L., Larsen, A.R., Skov, R.L., Peacock, S.J., Maskell, D.J. and Holmes, M.A. (2011) Methicillin-Resistant Staphylococcus aureus with a Novel mecA Homologue in Human and Bovine Populations in the UK and Denmark: A Descriptive Study. The Lancet Infectious Diseases, 11, 595-560. http://dx.doi.org/10.1016/S1473-3099(11)70126-8

[7]   Shore, A.C., Deasy, E.C., Slickers, P., Brennan G., O’Connell, B., Monecke, S., Ehricht, R. and Coleman, D.C. (2011) Detection of Staphylococcal Cassette Chromosome mec Type XI Carrying Highly Divergent mecA, mecI, mecRI, blaZ, and ccr Genes in Human Clinical Isolates of Clonal Complex 130 Methicillin-Resistant Staphylococcus aureus. Antimicrobial Agents and Chemotherapy, 55, 3765-3773. http://dx.doi.org/10.1128/AAC.00187-11

[8]   Bannerman, T.L. (2003) Staphylococcus, Micrococcus, and Other Catalase-Positive Cocci That Grow Aerobically. In: Murray, P.R., Baron, E.J., Jorgensen, J.H., Pfaller, M.A. and Yolken, R.H., Eds., Manual of Clinical Microbiology, 8th Edition, American Society for Microbiology, Washington DC, 384-404.

[9]   Selepak, S.T. and Witebsky, F.G. (1985) Inoculum Size and Lot-to-Lot Variation as Significant Variables in the Tube Coagulase Test for Staphylococcus aureus. Journal of Clinical Microbiology, 22, 835-837.

[10]   Rice, E.W., Fox, K.R., Nash, H.D., Read, E.J. and Smith, A.P. (1987) Comparison of Media for Recovery of Total Coliform Bacteria from Chemically Treated Water. Applied and Environmental Microbiology, 53, 1571-1573.

[11]   Pipes, W.O., Minnigh, H.A., Moyer, B. and Troy, M.A. (1986) Comparison of Clark’s Presence-Absence Test and the Membrane Filter Method for Coliform Detection in Potable Water Samples. Applied and Environmental Microbiology, 52, 439-443.

[12]   CLSI (2009) Performance Standards for Antimicrobial Susceptibility Testing; Nineteenth Informational Supplement. M100-S19, Clinical Laboratory Standards Institute, Wayne, PA.

 
 
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