ABC  Vol.4 No.6 , October 2014
New Approach for Isolation of Individual Caseins from Cow Milk by the Preparative Electrophoresis
Author(s) Andrii V. Iukalo
ABSTRACT
This paper carried out a comparative analysis of different types of electrophoretic systems which were used for the analysis of casein complex from cow milk (polyacrylamide gel electrophoresis: for the neutral and acidic native conditions, in gradient variant, the presence of sodium dodecyl sulphate or with including urea). Taking into attention the separation efficiency, complexity of electrophoretic system, the impact of system components, we have selected the anode system of the homogeneous gel in the presence of urea as the basis for the preparation of casein fractions. It also changed the composition and the structure of the electrophoretic apparatus. The changes allow purification of casein fractions up to several grams during one stage of treatment (for 5 hours). The purified casein fractions were tested for the homogeneity and have been recommended for using in the biomedical researches, including the processes of the formation of the bioactive peptides.

Cite this paper
Iukalo, A. (2014) New Approach for Isolation of Individual Caseins from Cow Milk by the Preparative Electrophoresis. Advances in Biological Chemistry, 4, 382-387. doi: 10.4236/abc.2014.46043.
References
[1]   Horne D.S. (2009) Casein Micelle Structure and Stability. In: Thompson, A. and Boland, M., Eds., Milk Proteins: From Expression to Food, Academic Press/Elsevier, Amsterdam, Boston, 133-162.

[2]   Hartmann, R. and Meisel, H. (2007) Food-Derived Peptides with Biological Activity: From Research to Food Applications. Current Opinion in Biotechnology, 18, 163-169.
http://dx.doi.org/10.1016/j.copbio.2007.01.013

[3]   Iukalo, A.V., Storozh, L.A. and Yukalo, V.G. (2012) Cow Milk Casein Complex (Bos Taurus) Proteins as Precursors of Bioactive Peptides. Biotechnology, 5, 21-33.

[4]   Gobetti, M., Minervini, F. and Grizzello, C. (2004) Angiotensin I-Converting-Enzyme-Inhibitory and Antimicrobial Bioactive Peptides. International Journal of Dairy Technology, 57, 173-188.
http://dx.doi.org/10.1111/j.1471-0307.2004.00139.x

[5]   Whitney, R. (1999) Proteins of Milk. Fundamentals of Dairy Chemistry. Aspen Publishers, Gaithersburg, 81-169.

[6]   Park, Y.W. (2009) Bioactive Components in Milk and Dairy Products. Wiley-Blackwell, Boston, 426 p.
http://dx.doi.org/10.1002/9780813821504

[7]   Yukalo, A., Yukalo, V. and Shynkaryk, M. (2009) Electrophoretic Separation of the Milk Protein. Proceeding of the International Conference on Bio and Food Electrotechnologies, Compiegne, 22-23 October 2009, 227-231.

[8]   Skalka, V.V., Savchuk, O.M. and Ostapchenko, L.I. (2010) Determination of Different Forms of Casein in Milk by Disc-Electrophoresis Method. Physics of Lieve, 18, 36-38.

[9]   Farrell Jr., H.M., Jimenez-Flores, R., Bleck, G.T., et al. (2004) Nomenclature of the Proteins of Cows’ Milk—Sixth Revision. Journal of Dairy Science, 87, 1641-1674.
http://dx.doi.org/10.3168/jds.S0022-0302(04)73319-6

[10]   Yukalo, V. (2005) Obtaining of Casein Protein Complex from Cow Milk. Nutracos, 5, 17-19.

[11]   Yukalo, V.G. (2001) Ion-Exchange Chromatography of Proteins of Casein Complex in Volume. Medical Chemistry, 4, 52-56.

[12]   Yukalo, V.G. (2001) Gel-Filtration of Proteins of Casein Complex. Medical Chemistry, 2, 35-38.

[13]   Yukalo, V.G., Javorskij, B.I., Storozh, L.A. and Solovodzins’ka, I.Y. (2007) Quantitative Electrophoretic Analysis of Casein Complex Proteins. Biology of Animals, 9, 295-298.

[14]   Kas’min, S.D. and Sherban, S.D. (1978) Determination of the Molecular Weight of Nuclear Proteins by Method of Disc-Electrophoresis in Gradient of Polyacrylamide Gel. Ukr. Biochem. Zhurn., 50, 108-115.

 
 
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