Escherichia coli is assumed to be
involved in inflammatory bowel disease (IBD) by many authors. The present Short
Report was aimed at analyzing E. coli population isolated from ileal samples collected from 4 CD and 3 non-CD
(control group) diagnosed pediatric patients. A total of 539 mucosa-associated E. coli strains were characterized by: Random Amplified Polymorphic
DNA (RAPD), adhesive and virulence factors, and for their phylogenetic
groups. A significant separation between
RAPD profiles of the two CD and non-CD cohorts (P < 0.0001), along with a
significant reduction of intra-species genomic variability in E. coli populations isolated from CD
group (P < 0.0001) was found as assessed by Dice index, indicating a
different selective pressure in CD intestinal habitat. A predominance of
phylogenetic group A was found in control subjects (P < 0.0001). Results on
adhesive and virulence factors evidenced peculiar genes significantly related
to CD E. coli populations (K1, IbeA)
(P < 0.0001) and to total DNA from biopsy specimens K1 (P < 0.0001). Results
suggest that among E. coli population,
particular variants may be favorite in the intestinal habitat of CD pediatric
patients. These genotype variants could represent the genetic background that,
via evolutionary phenomena driven by a persistent inflammatory state, may
evolve in Adherent Invasive Escherichia
coli (AIEC) like strains found in adult CD patients.
Cite this paper
Schippa, S. , Totino, V. , Marazzato, M. , Lepanto, M. , Santangelo, F. , Aleandri, M. , Gagliardi, A. , Longhi, C. , Pantanella, F. , Iebba, V. and Conte, M. (2014) Escherichia coli
Population-Based Study in Pediatric Crohn’s Disease. Advances in Microbiology
, 886-889. doi: 10.4236/aim.2014.413098
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