ABC  Vol.4 No.6 , October 2014
Differential Responses of Antioxidative System to Soil Water Shortage in Barley (Hordeum vulgare L.) Genotypes
ABSTRACT
Drought is one of the major factors limiting the yield and quality of crops in the world. The activity of antioxidative system to tolerate the drought stress is significant in plants. In the present study, the activities and isoform profiles of catalase (CАТ), ascorbate peroxidase (APX), glutathione reductase (GR), and superoxide dismutase (SOD) were analyzed in four barley genotypes grown under soil water restriction. Drought stress caused increase in the activities of CАТ and SOD in all studied genotypes, while APX activity decreased. The total GR activity increased substantially in genotypes K 2778 and St.Garabag 7 and decreased in No. 77 local and St.Pallidum 596 genotypes under conditions of severe water stress. No detectable differences were observed in the isoenzyme pattern (the appearance of a new isoenzymes and disappearance of another one) between control plants and those subjected to soil drought. However, intensification of corresponding isoforms in electrophoretic spectra was observed in stressed barley leaves relative to watered ones. The obtained results possibly suggest that antioxidant protection in barley plants under drought conditions could be attributed mainly to SOD and CAT.

Cite this paper
Huseynova, I. , Nasrullayeva, M. , Rustamova, S. , Aliyeva, D. and Aliyev, J. (2014) Differential Responses of Antioxidative System to Soil Water Shortage in Barley (Hordeum vulgare L.) Genotypes. Advances in Biological Chemistry, 4, 351-359. doi: 10.4236/abc.2014.46040.
References
[1]   Aranjuelo, I., Molero, G., Erice, G., Avice, J.C. and Nogués, S. (2011) Plant Physiology and Proteomics Reveals the Leaf Response to Drought in Alfalfa (Medicago sativa L.). Journal of Experimental Botany, 62, 111-123.
http://dx.doi.org/10.1093/jxb/erq249

[2]   Li, Z., Shi, P. and Peng, Y. (2013) Improved Drought Tolerance through Drought Preconditioning Associated with Changes in Antioxidant Enzyme Activities, Gene Expression and Osmoregulatory Solutes Accumulation in White Clover (Trifolium repens L.). Plant Omics, 6, 481-489.

[3]   Solomon, S., Qin, D., Manning, M., Alley, R.B., Berntsen, T., Bindoff, N.L., Chen, Z., Chidthaisong, A., Gregory, J.M., Hegerl, G.C., Heimann, M., Hewitson, B., Hoskins, B.J., Joos, F., Jouzel, J., Kattsov, V., Lohmann, U., Matsuno, T., Molina, M., Nicholls, N., Overpeck, J., Raga, G., Ramaswamy, V., Ren, J., Rusticucci, M., Somerville, R., Stocker, T.F., Whetton, P., Wood, R.A. and Wratt, D. (2007) Technical Summary. In: Solomon, S., Qin, D., Manning, M., Chen, Z., Marquis, M., Averyt, K.B., Tignor, M. and Miller, H.L. Eds., Climate Change 2007: The Physical Science Basis, Contribution of Working Group I to the Fourth Assessment Report of the Intergovernmental Panel on Climate Change, Cambridge University Press, Cambridge, United Kingdom and New York, USA, 996 p.

[4]   Faize, M., Burgos, L., Faize, L., Piqueras, A., Nicolas, E., Barba-Espin, G., Clemente-Moreno, M.J., Alcobendas, R., Artlip, T. and Hernandez, J.A. (2011) Involvement of Cytosolic Ascorbate Peroxidase and Cu/Zn-Superoxide Dismutase for Improved Tolerance against Drought Stress. Journal of Experimental Botany, 62, 2599-2613.
http://dx.doi.org/10.1093/jxb/erq432

[5]   Apel, K. and Hirt, H. (2004) Reactive Oxygen Species: Metabolism, Oxidative Stress, and Signal Transduction. Annual Review of Plant Biology, 55, 373-399.
http://dx.doi.org/10.1146/annurev.arplant.55.031903.141701

[6]   Joseph, B. and Jini, D. (2011) Development of Salt Stress-Tolerant Plants by Gene Manipulation of Antioxidant Enzymes. Asian Journal of Agricultural Research, 5, 17-27.

[7]   Mittler, R. (2002) Oxidative Stress, Antioxidants and Stress Tolerance. Trends in Plant Science, 7, 405-410.
http://dx.doi.org/10.1016/S1360-1385(02)02312-9

[8]   Ahmad, P., Jaleel, C.A., Salem, M.A., Nabi, G. and Sharma, S. (2010) Roles of Enzymatic and Nonenzymatic Antioxidants in Plants during Abiotic Stress. Critical Reviews in Biotechnology, 30, 161-175.
http://dx.doi.org/10.3109/07388550903524243

[9]   Mittler, R., Vanderauwera, S., Gollery, M. and Van Breusegem, F. (2004) Reactive Oxygen Gene Network of Plants. Trends in Plant Science, 9, 1360-1385.
http://dx.doi.org/10.1016/j.tplants.2004.08.009

[10]   Sarowar, S., Kim, E.N., Kim, Y.J., Ok, S.H., Kim, K.D., Hwang, B.K. and Shin, J.S. (2005) Overexpression of a Pepper Ascorbate Peroxidase-Like 1 Gene in Tobacco Plants Enhances Tolerance to Oxidative Stress and Pathogens. Plant Science, 169, 55-63.
http://dx.doi.org/10.1016/j.plantsci.2005.02.025

[11]   Shaaltiel, Y., Chua, N.H., Gepstein, S. and Gressel, J. (1988) Dominant Pleiotropy Controls Enzymes Co-Segregating with Paraquet Resistance in Conyza bonariensis. Theoretical and Applied Genetics, 75, 850-856.

[12]   Amini, R. (2013) Drought Stress Tolerance of Barley (Hordeum vulgare L.) Affected by Priming with PEG. International Journal of Farming and Allied Sciences, 2, 803-808.

[13]   Fayez, K.A. and Bazaid, S.A. (2014) Improving Drought and Salinity Tolerance in Barley by Application of Salicylic Acid and Potassium Nitrate. Journal of the Saudi Society of Agricultural Sciences, 13, 45-55.
http://dx.doi.org/10.1016/j.jssas.2013.01.001

[14]   Ashraf, M. (2010) Inducing Drought Tolerance in Plants: Recent Advances. Biotechnology Advances, 28, 169-183.
http://dx.doi.org/10.1016/j.biotechadv.2009.11.005

[15]   Zhang, J. and Kirkham, M.B. (1994) Drought-Stress-Induced Changes in Activities of Superoxide Dismutase, Catalase and Peroxidase in Wheat Species. Plant and Cell Physiology, 35, 785-791.

[16]   Fu, J. and Huang, B. (2001) Involvement of Antioxidants and Lipid Peroxidation in the Adaptation of Two Cool-Season Grasses to Localized Drought Stress. Environmental and Experimental Botany, 45, 105-114.
http://dx.doi.org/10.1016/S0098-8472(00)00084-8

[17]   Shao, H.B., Liang, Z.S., Shao, M.A. and Su, Q. (2005) Dynamic Changes of Antioxidative Enzymes of 10 Wheat Genotypes at Soil Water Deficits. Colloids and Surfaces B: Biointerfaces, 42, 187-195.
http://dx.doi.org/10.1016/j.colsurfb.2005.02.007

[18]   Polesskaya, O.G. (2007) Plant Cell and Reactive Oxygen Species. Yermakov, I.P., Ed., Moscow, 140.

[19]   Kumar, C.N. and Knowles, N. (1993) Changes in Lipid Peroxidation and Lipolytic and Free-Radical Scavenging Enzyme during Aging and Sprouting of Potato (Solanum tuberosum L.) Seed-Tubers. Plant Physiology, 102, 115-124.
http://dx.doi.org/10.1104/pp.102.1.115

[20]   Nakano, Y. and Asada, K. (1981) Hydrogen Peroxide Is Scavenged by Ascorbate-Specific Peroxidase in Spinach Chloroplasts. Plant and Cell Physiology, 22, 867-880.

[21]   Yannarelli, G.G., Fernández-Alvarez, A.J., Santa-Cruz, D.M. and Tomaro, M.L. (2007) Glutathione Reductase Activity and Isoforms in Leaves and Roots of Wheat Plants Subjected to Cadmium Stress. Phytochemistry, 68, 505-512.
http://dx.doi.org/10.1016/j.phytochem.2006.11.016

[22]   Sedmak, J.J. and Grossberg, S.E. (1977) A Rapid, Sensitive, and Versatile Assay for Protein Using Coomassie Brilliant Blue G250. Analytical Biochemistry, 79, 544-552.
http://dx.doi.org/10.1016/0003-2697(77)90428-6

[23]   Laemmli, U.K. (1970) Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4. Nature, 227, 680-685.
http://dx.doi.org/10.1038/227680a0

[24]   Mittler, R, and Zilinskas, B.A. (1993) Detection of Ascorbate Peroxidase Activity in Native Gels by Inhibition of the Ascorbate-Dependent Reduction of Nitroblue Tetrazolium. Analytical Biochemistry, 212, 540-546.

[25]   Woodbury, W., Spencer, A.K. and Stahmann, M.A. (1971) An Improved Procedure Using Ferricyanide for Detecting Catalase Isoenzymes. Analytical Biochemistry, 44, 301-305.
http://dx.doi.org/10.1016/0003-2697(71)90375-7

[26]   Mhamdi, A., Queval, G., Chaouch, S., Vanderauwera, S., Van Breusegem, F. and Noctor, G. (2010) Catalase Function in Plants: A Focus on Arabidopsis Mutants as Stress-Mimic Models. Journal of Experimental Botany, 61, 4197-4220.
http://dx.doi.org/10.1093/jxb/erq282

[27]   Najami, N., Janda, T., Barriah, W., Kayam, G., Tal, M., Guy, M. and Volokita, M. (2008) Ascorbate Peroxidase Gene Family in Tomato: Its Identification and Characterization. Molecular Genetics and Genomics, 279, 171-182.
http://dx.doi.org/10.1007/s00438-007-0305-2

[28]   Gill, S.S. and Tuteja, N. (2010) Reactive Oxygen Species and Antioxidant Machinery in Abiotic Stress Tolerance in Crop Plants. Plant Physiology and Biochemistry, 48, 909-930.
http://dx.doi.org/10.1016/j.plaphy.2010.08.016

[29]   Badiani, M., De Biasi, M.G., Colognola, M. and Artemi, F. (1990) Catalase, Peroxidase and Superoxide Dismutase Activities in Seedlings Submitted to Increasing Water Deficit. Agrochimica, 34, 90-102.

[30]   Yoshimura, K., Yabuta, Y., Ishikawa, T. and Shigeoka, S. (2000) Expression of Spinach Ascorbate Peroxidase Isoenzymes in Response to Oxidative Stresses. Plant Physiology, 123, 223-234.
http://dx.doi.org/10.1104/pp.123.1.223

[31]   Saruhan, N., Terzi, N., Saglam, A. and Kadioglu, A. (2009) The Relationship between Leaf Rolling and Ascorbate-Glutathione Cycle Enzymes in Apoplastic and Symplastic Areas of Ctenanthe setosa Subjected to Drought Stress. Biological Research, 42, 315-326.
http://dx.doi.org/10.4067/S0716-97602009000300006

[32]   Romero-Puertas, M.C., Corpas, F.J., Sandalio, L.M., Leterrier, M., Rodriguez-Serrano, M., Del Río, L.A., et al. (2006) Glutathione Reductase from Pea Leaves: Response to Abiotic Stress and Characterization of the Peroxisomal Isozyme. New Phytologist, 170, 43-52.

[33]   Alscher, R.G., Donahue, J.L. and Cramer, C.L. (2002) Reactive Oxygen Species and Antioxidants: Relationships in Green Cells. Physiologia Plantarum, 100, 224-233.
http://dx.doi.org/10.1111/j.1399-3054.1997.tb04778.x

[34]   Raychaudhuri, S.S. and Deng, X.W. (2000) The Role of Superoxide Dismutase in Combating Oxidative Stress in Higher Plants. The Botanical Review, 66, 89-98.
http://dx.doi.org/10.1007/BF02857783

[35]   Brou, Y.C., Zeze, A., Diouf, O. and Eyletters, M. (2007) Water Stress Induces Overexpression of Superoxide Dismutases That Contribute to the Protection of Cowpea Plants against Oxidative Stress. African J. Biotech., 6, 1982-1986.

[36]   Kim, S.Y., Lim, J.H., Park, M.R., Kim, Y.J., Park, T.I., Seo, Y.W., Choi, K.G. and Yun, S.J. (2005) Enhanced Antioxidant Enzymes Are Associated with Reduced Hydrogen Peroxide in Barley Roots under Saline Stress. Journal of Biochemistry and Molecular Biology, 38, 218-224.
http://dx.doi.org/10.5483/BMBRep.2005.38.2.218

[37]   Domanskaya, I.N., Budakova, E.A., Samovich, T.V., Spivak, E.A. and Shaligo, N.V. (2009) Activities of the Antioxidant Enzymes in Green Seedlings of Barley (Hordeum vulgare) under Drought Conditions. Proceedings of the Academy of Sciences of Belarus (Series of Biological Sciences), 4, 45-49.

 
 
Top