AJPS  Vol.5 No.20 , September 2014
An Assessment of Genetic Relatedness between Soybean [Glycine max (L.) Merrill] Cultivars Using SSR Markers
Abstract: In this present investigation, simple sequence repeat (SSR) analysis was used to determine the genetic relatedness among 32 soybean (Glycine max (L.) Merr.) cultivars from NRC for soybean, Madhya Pradesh. Among them 10 primer pairs showed 100% amplification. Mainly three primer pairs could amplify polymorphic SSRs from all of these cultivars. The polymorphic information content (PIC) among cultivars varied from 0.21 (S26) to 0.83 (S27) with an average of 0.51. Pairwise coefficients of genetic similarity between all genotypes ranged from 0.76 to 1.00. Unweighted pair-group method arithmetic average (UPGMA) analysis allocated the cultivars in 2 major clusters or groups and 6 sub-clusters. Of the two major clusters one contained 20 cultivars and the other contained 12 cultivars. The largest cluster was again divided into three sub-clusters I, II and III with 12, 2 and 6 cultivars respectively and the smallest cluster was divided into three sub-clusters IV, V, VI containing 7, 4 and 2 cultivars respectively. These results suggest that SSR markers are efficient for measuring genetic relatedness among soybeans irrespective of a wide agro-climatic zone. Genetic relationship assessments among soybean cultivars in India could provide useful information for efficient utilization of these materials, especially for widening the genetic base.
Cite this paper: Ghosh, J. , Ghosh, P. and Choudhury, P. (2014) An Assessment of Genetic Relatedness between Soybean [Glycine max (L.) Merrill] Cultivars Using SSR Markers. American Journal of Plant Sciences, 5, 3089-3096. doi: 10.4236/ajps.2014.520325.

[1]   Singh, R.J. and Hymowitz, T. (1999) Soybean Genetic Resources and Crop Improvement. Genome, 42, 605-616.

[2]   Mathur, S. (2004) Soybean the Wonder Legume. Beverage Food World, 31, 61-62.

[3]   Gautam, P. and Pant, L.M. (2004) Soybean: A “Miracle Bean” for Uttaranchal. Indian Farmers Digest.

[4]   Brown-Guedira, G.L., Thompson, J.A., Nelson, R.L. and Warburton, M.L. (2000) Evaluation of Genetic Diversity of Soybean Introductions and North American Ancestors Using RAPD and SSR Markers. Crop Science, 40, 815-823.

[5]   Narvel, J.M., Fehr, W.R., Chu, W.C., Grant, D. and Shoemaker, R.C. (2000) Simple Sequence Repeat Diversity among Soybean Plant Introductions and Elite Genotypes. Crop Science, 40, 1452-1458.

[6]   Fu, Y.B., Peterson, G.W. and Morrison, M.J. (2007) Genetic Diversity of Canadian Soybean Cultivars and Exotic Germplasm Revealed by Simple Sequence Repeat Markers. Crop Science, 47, 1947-1954.

[7]   Abe, J., Xu, D.H., Suzuki, Y., Kanazawa, A. and Shimamoto, Y. (2003) Soybean Germplasm Pools in Asia Revealed by Nuclear SSRs. Theoretical and Applied Genetics, 106, 445-453.

[8]   Xie, H., Guan, R., Chang, R. and Qiu, L. (2005) Genetic Diversity of Chinese Summer Soybean Germplasm Revealed by SSR Markers. Chinese Science Bulletin, 50, 526-535.

[9]   Wang, L., Guan, R., Liu, Z.X., Chang, R. and Qiu, L. (2006) Genetic Diversity of Chinese Cultivated Soybean Revealed by SSR Markers. Crop Science, 46, 1032-1038.

[10]   Li, L., Wang, X., Gai, J. and Yu, D. (2008) Isolation and Characterization of a Seed-Specific Isoform of Microsomal Omega-6 Fatty Acid Desaturase Gene (FAD2-1B) from Soybean. DNA Sequence, 19, 28-36.

[11]   Wang, F., Zhao, T.J., Yu, D.Y., Chen, S.Y. and Gai, J.Y. (2008) Inheritance and QTL Analysis of Submergence Tolerance at Seedling Stage in Soybean [Glycine max (L.) Merr.]. Acta Agronomica Sinica, 34, 748-753.

[12]   Yoon, M., Lee, J., Kim, C., Kang, J., Cho, E. and Baek, H. (2009) DNA Profiling and Genetic Diversity of Korean Soybean (Glycine max (L.). Merrill) Landraces by SSR Markers. Euphytica, 165, 69-77.

[13]   Bonato, A.L.V., Calvo E.S., Geraldi, I.O. and Arias, C.A.A. (2006) Genetic Similarity among Soybean (Glycine max (L). Merrill) Cultivars Released in Brazil Using AFLP Markers.

[14]   Chotiyarnwong, O., Chatwachirawong, P., Chanprame, S. and Srinives, P. (2007) Evaluation of Genetic Diversity in Thai Indigenous and Recommended Soybean Varieties by SSR Markers. Thai Journal of Agricultural Science, 40, 119-126.

[15]   Song, Q.J., Quigley, C.V., Nelson, R.L., Carter, T.E., Boerma, H.R., Strachan, J.L. and Cregan, P.B. (1999) A Selected Set of Trinucleotide Simple Sequence Repeat Markers for Soybean Cultivar Identification. Plant Varieties and Seeds, 12, 207-220.

[16]   Cregan, P.B., Jarvik, T., Bush, A.L., Shoemaker, R.C., Lark, K.G., Kahler, A.L., Kaya, N., Van Toai, T.T., Lohnes, D. G., Chung, J. and Specht, J.E. (1999) An Integrated Genetic Linkage Map of the Soybean Genome. Crop Science, 39, 1464-1490.

[17]   Cregan, P.B., Mudge, J., Fickus, E.W., Marek, L.F., Danesh, D., Denny, R., Shoemaker, R.C., Matthews, B.F., Jarvik, T. and Young, N.D. (1999) Targeted Isolation of Simple Sequence Repeat Markers through the Use of Bacterial Artificial Chromosomes. Theoretical and Applied Genetics, 98, 919-928.

[18]   Ravikumar, M. and Narayanasamy, S. (1999) Identification of Soybean Varieties Based on Seed and Morphological Characters. Current Research, 28, 50-52.

[19]   Borthakur, P.N. (1989) Exploiting Biological Diversity for Breeding Crop Plants for North-Easternregion. Proceedings of Indian National Science Academy, Biological Sciences, 55, 185-192.

[20]   Kisha, T.J., Dher, B.W., Hoyt, J.M. and Sneller, C.H. (1998) Genetic Diversity among Soybean Plant Introduction and North American Germplasm. Crop Science, 38, 1669-1680.

[21]   Murray, M.G. and Thompson, W.F. (1980) Rapid Isolation of High Molecular Weight Plant DNA. Nucleic Acids Research, 8, 4321-4325.

[22]   Sambrook, J. and Russed, D.W. (2001) Molecular Cloning. A Laboratory Manual. 3rd Edition, Cold Spring Harbor Laboratory Press, New York.

[23]   Sambrook, J., Frotsch, E.F. and Maniatis, T. (1989) Molecular Cloning. A Laboratory Manual. 2nd Edition, Cold Spring Harbor, New York.

[24]   Rana, M.K., Archak, S. and Singh, R. (2012) An E-Manual on “Molecular Marker Techniques for Genotype Identification”. NBPGR, Pusa Campus, New Delhi.

[25]   Asif, M., Mehboob-ur-Rahman, Mirza, I.J. and Yusuf, Z. (2008) High Resolution Metaphore Agarose Gel Electrophoresis for Genotyping with Microsatellite Markers. Pakistan Journal of Agricultural Sciences, 45, 75-79.

[26]   Rohlf, F.J. (2000) NTSYS-pc: Numerical Taxonomy and Multivariate Analysis System, Version 2.2. Exeter Software. Setauket, New York.

[27]   Jaccard, P. (1908) Nouvelles recherches sur la distribution florale. Bulletin de la Société Vaudoise des Sciences Naturelles, 44, 223-270.

[28]   Tantasawat, P., Trongchuen, J., Prajongjai, T., Jenweerawat, S. and Chaowiset, W. (2011) SSR Analysis of Soybean (Glycine max (L.) Merr.) Genetic Relationship and Variety Identification in Thailand. American Journal of Cultural Sociology, 5, 283-290.

[29]   Chowdhury, A.K., Srinives, P., Tongpamnak, P., Saksoong, P. and Chatwachirawong, P. (2002) Genetic Relationship among Exotic Soybean Introductions in Thailand: Consequence for Varietal Registration. Science Asia, 28, 227-239.