ABSTRACT Traditionally used cotton-based bandaging materials have several disadvantages which can be overcome by using another fabric structure – nonwoven fabric. Moreover, these materials are more spongeous which increases their sorption capacity. The new bandaging material developed by the Institute of Physics of Strength and Material Science of the Siberian Branch of the Russian Academy of Sciences has even better sorption capacity with improved sorption properties. Its sorption capacity has been increased by means of an additional introduction of porous aluminum hydrate particles into fabric. It is important that a bandaging material has a good biocompatibility and does not have any cytotoxic effect on cells and tissues. Here it is present results of the study of the material's direct contact and indirect cytotoxicity assays in comparison with cotton gauze. It have found that in the direct contact of nonwoven polymeric fibrous bandaging material (NPFBM) with cells for 24 hours of cultivation no changes in cell morphology take place, nor does the amount of dead cells increase. These conclusions have been made by means of both a visual examination and an МТТ assay. The NPFBM extract did not have any cytotoxic effect on the tested cells either. The obtained results allow us to make a conclusion that the NPFBM complies with the international standard ISO 10993-5, which is applied to medical goods, and can from now on be applied in the treatment of infected wounds in clinical practice.
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nullA. Dygai, L. Ogorodova, S. Psakhie, Y. Belsky, N. Belska, M. Danilets, A. Ligatcheva and A. Сhurin, "A Study of the Cytotoxicity of a New Nonwoven Polymeric Fibrous Bandaging Material In-Vitro," Journal of Biomaterials and Nanobiotechnology, Vol. 2 No. 3, 2011, pp. 234-238. doi: 10.4236/jbnb.2011.23029.
 T. D. Turner, “Hospital usage of absorbent dressings”, Pharmaceutical Journal, V.222, 1979, pp. 421-424.
 V. J. Jones, “The use of gauze: will it ever change?”, International Wound Journal, V.3, 2006, pp. 79–88.
 X. Wang, Y. Yan, R. Zhang “A Comparison of Chitosan and Collagen Sponges as Hemostatic Dressings”, Journal of Bioactive and Compatible Polymers, No.21, 2006, pp. 39-54.
 G. Chaby, P. Senet, M. Vaneau et al. “Dressings for Acute and Chronic Wounds”, Arch Dermatol, V.143, No.10, 2007, pp. 1297-1304.
 T. S. Stashak, E. Farstvedt, A. Othic “Update on Wound Dressings: Indications and Best Use”, Clin Tech Equine Pract, No.3, 2004, pp.148-163.
 M. Ip, S. L. Lui, V. Poon, et al., “Antimicrobial activities of silver dressings: an in vitro comparison”, J Med Microbiol, No. 55, 2006, pp. 59-63.
 Patent (RU) N 2397781 from 27.08.2010 The Nonwoven Material of Medical Appointment Possessing Wound Healing, Antibacterial and Antiviral Activity and Bandaging Material on its Basis/Dygaj A.M., Lerner M. I, Novitsky V.V., Ogorodova L.M., Psakhie S.G., Churin A.A. [in Russian].
 ISO 10993-5, “Biological Evaluation of Medical Devices, Part 5: Tests for Cytotoxicity, in Vitro Methods,” International Standardization Organisation, Geneva, 1992.
 T. R. Mosmann, “Rapid Colorimetric Assay for Cellular Growth and Survival: Application to Proliferation and Cytotoxicity Assays,” Journal of Immunological Methods, No. 5, 1983, pp. 55-63.