JBNB  Vol.2 No.3 , July 2011
Rapid and Facile Purification of Apolipoprotein A-I from Human Plasma Using Thermoresponsive Nanoparticles
ABSTRACT
Nanoparticles can be used to purify proteins from plasma. We report here the purification of apolipoprotein A-I (apoA-I) with high specificity from human plasma using copolymeric nanoparticles. We present an optimized protocol using 50:50 NiPAM:BAM copolymer nanoparticles with thermo-responsive properties as an affinity resin. Repeated pelleting and washing of nanoparticle-captured apoA-I is achieved through temperature cycling. The protein is then eluted using urea followed by an ion exchange step for protein concentration and depletion of nanoparticles.

Cite this paper
nullM. Lundqvist, T. Berggård, E. Hellstrand, I. Lynch, K. Dawson, S. Linse and T. Cedervall, "Rapid and Facile Purification of Apolipoprotein A-I from Human Plasma Using Thermoresponsive Nanoparticles," Journal of Biomaterials and Nanobiotechnology, Vol. 2 No. 3, 2011, pp. 258-266. doi: 10.4236/jbnb.2011.23033.
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