AJPS  Vol.5 No.20 , September 2014
Phenolic Compound Profiles of Two Common Beans Consumed by Rwandans
Abstract: Legumes are high-protein, medium-energy and micronutrient-rich food consumed in many parts of the world including Africa. This study evaluated the levels of specific phenolic compounds in three legumes. Two varieties of the common bean, (Phaseolus vulgaris L.) soybeans (Glycine max L.), and peas (Pisum sativum L.) from Rwanda were analyzed using high performance liquid chromatography with diode array detection. The phenolic compounds were identified by comparison to the chromatographic retention times and UV spectra of known reference compounds. This study results clearly shows the presence of 11 different phenolic compounds in common beans: gallic acid, (+)-catechin, (–)-epicatechin, caffeic acid, o-coumaric acid, chlorogenic acid, quercetin, 4-hydrobenzoic acid, syringic acid, ferulic acid and vanillic acid. The concentration ranged from 0.59 to 2.27 mg/kg for epicatechin. High levels of catechin (13.5 to 57.9 mg/kg) ferulic acid (26.1 to 47.6 mg/kg) were also observed. Therefore, the results of this study show that Rwandan common beans are a good source of phenolic acids in particular catechins and ferulic acid.
Cite this paper: Joseph, O. , Phelomene, M. , Helene, N. , Valens, H. , Patrick, O. , Thavarajah, D. and Thavarajah, P. (2014) Phenolic Compound Profiles of Two Common Beans Consumed by Rwandans. American Journal of Plant Sciences, 5, 2943-2947. doi: 10.4236/ajps.2014.520310.

[1]   Broughton, W.J., Hernandez, G., Blair, M., Beebe, S., Gepts, P. and Vanderleyde, J. (2003) Beans (Phaseolus spp.)— Model Food Legumes. Plant and Soil, 252, 55-128.

[2]   Vivanco, J.M., Bais, H.P., Vepachedu, R., Gilroy, S. and Callaway, R.M. (2003) Allelopathy and Exotic Plant Invasion: From Molecules and Genes to Species Interactions. Science, 13, 77-80.

[3]   Maria, A., Janeiro, P. and Oliveira, B. (2004) Catechin Electrochemical Oxidation Mechanisms. Analytica Chimica Acta, 518, 109-115.

[4]   Woods, R.K., Raven, J.M., Wolfe, R., Ireland, P.D., Thien, F.C.K. and Abramson, M.J. (2003) Food and Nutrient Intakes and Asthma Risk in Young Adults. American Journal of Clinical Nutrition, 78, 414-421.

[5]   Oatway, L., Vasanthan, T. and Helm, J. (2001) Phytic Acid. Food Reviews International, 17, 419-431.

[6]   MINAGRI (2012) CIA World Factbook. MINAGRI, Rwanda.

[7]   Robbins, J.R. and Bean, S.R. (2004) Development of a Quantitative High Performance Liquid Chromatography-Photodiode Array Detection Measurement System for Phenolic Acids. Journal of Chromatography A, 1038, 97-105.

[8]   Escarpa, A. and Gonzaez, M.C. (2000) Identification and Quantitation of Phenolics from Green Beans by High-Performance Liquid Chromatography. Chromatographia, 52, 33-38.

[9]   Kofink, M., Papagiannopoulos, M. and Galensa, R. (2007) (-)-Catechin in Cocoa and Chocolate: Occurrence and Analysis of an Atypical Flavan-3-ol Enantiomer. Molecule, 12, 1274-1288.

[10]   Sosulski, F.W. and Dabroski, K.J. (1984) Composition of Free and Hydrolysable Phenolic Acids in the Flours and Hulls of Ten Legume Species. Journal of Agriculture and Food Chemistry, 32, 131-133.

[11]   Luthria, D.L. and Pastor-Corrales, M.A. (2006) Phenolic Acids Content of Fifteen Dry Edible Bean (Phaseolus vulgaris L.) Varieties. Journal of Food Composition and Analysis, 19, 205-211.

[12]   Dixon, R.A. and Paiva, N.L. (1995) Stress-Induced Phenylpropanoid Metabolism. Plant Cell, 7, 1085-1097.

[13]   Ninfali, P. and Bacchiocca, M. (2003) Polyphenols and Antioxidant Capacity of Vegetables under Fresh and Frozen Conditions. Journal of Agricultural and Food Chemistry, 51, 2222-2226.

[14]   Hakkinen, S. and Torronen, R. (2000) Content of Flavonols and Selected Phenolic Acids in Strawberries Vaccinium Species: Influence of Cultivar, Cultivation Site and Technique. Food Reviews International, 33, 517-524.