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 JAMP  Vol.2 No.10 , September 2014
Effect of Temperature, pH and Water Activity on Penicillium digitatum Growth
Abstract: Growth curves fitted Penicillium digitatum were used to analyze the effect of temperature, pH and aw on their growth. To asses observance of independent variable effects on all growth parameters (λ, latency time, μ, growth rate and ymax maximal growth) a slow growth strain of P. digitatum was employed. Growth curves were obtained at different conditions of temperature (10?C - 40?C), pH (3.0 - 7.0) and water activity (0.800 - 0.990) and growth parameters were calculated by fitting of logistic model. Polynomial models were made by linear regression and all terms (linear, quadratic and interactive effects) were statistically significant (p < 0.001). All growth parameters, including maximal growth were affected by environmental conditions; pH effect was more important on maximal growth than that on lag time or growth rate. In some aspects results and modeling behavior of P. digitatum, are very similar to modeling of bacterial growth.
Cite this paper: Carrillo-Inungaray, M. , Hidalgo-Morales, M. , Rodríguez-Jimenes, G. , García-Alvarado, M. , Ramírez-Lepe, M. , Munguía, A. and Robles-Olvera, V. (2014) Effect of Temperature, pH and Water Activity on Penicillium digitatum Growth. Journal of Applied Mathematics and Physics, 2, 930-937. doi: 10.4236/jamp.2014.210105.
References

[1]   Cuppers, H.G., Oomes, S. and Brul, S. (1997) A Model for the Combined Effects of Temperature and Salt Concentration on Growth Rate of Food Spoilage Molds. Applied Environmental Microbiology, 6310, 3764-3769.

[2]   Lebert, I., Robles Olvera, V. and Lebert, A. (2000) Application of Polynomial Models to Predict Growth of Mixed Cultures of Pseudomonas spp. and Listeria in Meat. International Journal Food Microbiology, 61, 27-39.
http://dx.doi.org/10.1016/S0168-1605(00)00359-7

[3]   Mellefont, L.A., McMeekin, T.A. and Ross, T. (2003) Performance Evaluation of a Model Describing the Effects of Temperature, Water Activity, pH and Lactic Acid Concentration on the Growth of Escherichia coli. International Journal Food Microbiology, 821, 45-58. http://dx.doi.org/10.1016/S0168-1605(02)00253-2

[4]   Marcet-Houben, M., Ballester, A.R., De la Fuente, B., Harries, E., González-Candelas, J.M.L. and Gabaldón, T. (2012) Genome Sequence of the Necrotrophic Fungus Penicillium digitatum, the Main Postharvest Pathogen of Citrus. BMC Genomics, 13, 646.

[5]   Gatto, M.A., Ippolito A., Linsalata, V., Cascatano, N.A., Nigro, F., Vanadia, S. and Di Venere, D. (2011) Activity of Extracts from Wild Edible Herbs against Postharvest Fungal Diseases of Fruit and Vegetables. Postharvest Biology and Technology, 61, 72-82. http://dx.doi.org/10.1016/j.postharvbio.2011.02.005

[6]   Guynot, M.E., Marin, S., Sanchis, V. and Ramos, A.J. (2003) Modified Atmosphere Packaging for Prevention of Mold Spoilage of Bakery Products with Different pH and Water Activity Levels. Journal of Food Protection, 6610, 1864-1872.

[7]   Sautour, M., Dantigny, P., Divies, C. and Bensoussan, M. (2001) A Temperature-Type Model for Describing the Relationship between Fungal Growth and Water Activity. International Journal Food Microbiology, 67, 63-69.
http://dx.doi.org/10.1016/S0168-1605(01)00471-8

[8]   Valik, L., Baranyi, J. and Gorner, F. (1999) Predicting Fungal Growth: The Effect of Water Activity on Penicillium roqueforti. International Journal Food Microbiology, 47, 141-146.
http://dx.doi.org/10.1016/S0168-1605(98)00201-3

[9]   Abellana, M., Benedi, J., Sanchis, V. and Ramos, A.J. (1999) Water Activity and Temperature Effects on Germination and Growth of Eurotium amstelodami, E. chevalieri and E. herbariorum Isolates from Bakery Products. Journalof Applied Microbiology, 873, 371-380. http://dx.doi.org/10.1046/j.1365-2672.1999.00828.x

[10]   Sautour, M., Soares-Mansur, C., Divies, C., Bensoussan, M. and Dantigny, P. (2002) Comparison of the Effects of Temperature and Water Activity on Growth Rate of Food Spoilage Moulds. Journal of Industrial Microbiology and Biotechnology, 286, 311-315. http://dx.doi.org/10.1038/sj.jim.7000248

[11]   Gock, M.A., Hocking, A.D., Pitt, J.I. and Poulos, P.G. (2003) Influence of Temperature, Water Activity and pH on Growth of Some Xerophilic Fungi International Journal Food Microbiology, 811, 11-19.
http://dx.doi.org/10.1016/S0168-1605(02)00166-6

[12]   Panagou, E.Z., Skandamis, P.N. and Nychas, G.J.E. (2003) Modeling the Combined Effect of Temperature, pH and aw on the Growth Rate of Monascus ruber, a Heat-Resistant Fungus Isolated from Green Table Olives. Journal of Applied Microbiology, 94, 146-156. http://dx.doi.org/10.1046/j.1365-2672.2003.01818.x

[13]   Battey, A.S., Duffy, S. and Schaffner, D.W. (2001) Modeling Mould Spoilage in Cold-Filled Ready-to-Drink Beverages by Aspergillus niger and Penicillium spinulosum. Food Microbiology, 185, 521-529.
http://dx.doi.org/10.1006/fmic.2001.0438

[14]   Daferera, D.J., Ziogas, B.N. and Polissiou, M.G. (2000) GC-MS Analysis of Essential Oils from Some Greek Aromatic Plants and Their Fungitoxicity on Penicillium digitatum. Journal of Agricultural Food Chemistry, 486, 2576-2581.
http://dx.doi.org/10.1021/jf990835x

[15]   López-Malo, A., Alzamora, S.M. and Argaiz, A. (1998) Vanillin and pH Synergistic Effects on Mold Growth. Journal of Food Science, 631, 143-146. http://dx.doi.org/10.1111/j.1365-2621.1998.tb15695.x

[16]   Matamoros-Leon, B., Argaiz, A. and López-Malo, A. (1999) Individual and Combined Effects of Vanillin and Potassium Sorbate on Penicillium digitatum, Penicillium glabrum, and Penicillium italicum Growth. Journal of Food Protection, 625, 540-542.

[17]   Plaza, P., Usall, J., Teixidó, N. and Vinas, I. (2004) Effect of Water Activity and Temperature on Competing Abilities of Common Postharvest Citrus Fungi. International Journal Food Microbiology, 901, 75-82.
http://dx.doi.org/10.1016/S0168-1605(03)00292-7

[18]   Plaza, P., Usall, J., Teixidó, N. and Vinas, I. (2003) Effect of Water Activity and Temperature on Germination and Growth of Penicillium digitatum, P. italicum and Geotrichum candidum. Journal of Applied Microbiology, 94, 549-554. http://dx.doi.org/10.1046/j.1365-2672.2003.01909.x

[19]   Montgomery, D.C. (2000) Design and Analysis of Experiments. 5th Edition, John Wiley & Sons, New York.

[20]   Norrish, R.S. (1966) An Equation for the Activity Coefficients and Equilibrium Relative Humidities of Water in Confectionery Syrups. International Journal of Food Science & Technology, 1, 25-39.
http://dx.doi.org/10.1111/j.1365-2621.1966.tb01027.x

[21]   Ross, K.D. (1975) Estimation of Water Activity in Intermediate Moisture Foods. Food Technology, 29, 26-34.

[22]   Samson, A., Hoekstra, E.S., Frisvad, J.C. and Filtenborg, O. (1995) Introduction to Food-Borne Fungi. 4th Edition, Centraalbureau voor schimmelcultures, Baarn and Delft, The Netherlands.

[23]   Fustier, P., Lafond, A., Champagne, C.P. and Lamarche, F. (1998) Effect of Inoculation Techniques and Relative Humidity on the Growth of Molds on the Surfaces of Yellow Layer Cakes. Applied Environmental Microbiology, 641, 192-196.

[24]   Ross, T. (1996) Indices for Performance Evaluation of Predictive Models in Food Microbiology. Journal of Applied Bacteriology, 815, 501-508.

[25]   Baranyi, J., Pin, C. and Ross, T. (1999) Validating and Comparing Predictive Models. International Journal of Food Microbiology, 483, 159-166. http://dx.doi.org/10.1016/S0168-1605(99)00035-5

[26]   López, S., Prieto, M., Dijkstra, J., Dhanoa, M.S. and France, J. (2004) Statistical Evaluation of Mathematical Models for Microbial Growth. International Journal of Food Microbiology, 963, 289-300.
http://dx.doi.org/10.1016/j.ijfoodmicro.2004.03.026

[27]   Dantigny, P., Guilmart, A., Radoi, F., Bensoussan, M. and Zwietering, M. (2005) Modelling the Effect of Ethanol on Growth Rate of Food Spoilage Moulds. International Journal of Food Microbiology, 98, 261-269.

[28]   Neumeyer, K., Ross, T. and Mcmeekin, T.A. (1997) Development of a Predictive Model to Describe the Effects of Temperature and Water Activity on the Growth of Spoilage Pseudomonads. International Journal of Food Microbiology, 38, 45-54. http://dx.doi.org/10.1016/S0168-1605(97)00089-5

[29]   McKellar, R.C. and Knight, K. (2000) A Combined Discrete-Continuous Model Describing the Lag Phase of Listeria monocytogenes. International Journal of Food Microbiology, 54, 171-180.
http://dx.doi.org/10.1016/S0168-1605(99)00204-4

[30]   Baranyi, J. and Roberts, T.A. (1994) A Dynamic Approach to Predicting Bacterial Growth in Food. International Journal of Food Microbiology, 23, 277-294. http://dx.doi.org/10.1016/0168-1605(94)90157-0

[31]   Parra, R. and Magan, N. (2004) Modelling the Effect of Temperature and Water Activity on Growth of Aspergillus niger Strains and Applications for Food Spoilage Moulds. Journal of Applied Microbiology, 972, 429-438.
http://dx.doi.org/10.1111/j.1365-2672.2004.02320.x

 
 
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