FNS  Vol.5 No.18 , September 2014
Probiotic Lactic Acid Bacteria: A Review
Abstract: Lactic acid bacteria (LAB) play a critical role in food production and health maintenance. There is an increasing interest in these species to reveal the many possible health benefits associated with them. The actions of LAB are species and strain specific, and depend on the amount of bacteria available in the gastrointestinal tract. Consumers are very concerned of chemical preservatives and processed foods. However, products with or processed with LAB are accepted as a natural way to preserve food and promote health. This paper aimed to review the recent data in regard to the role of probiotic LAB in the preservation of foods, in the immunomodulation in the gastrointestinal tract, and in its health benefits
Cite this paper: Quinto, E. , Jiménez, P. , Caro, I. , Tejero, J. , Mateo, J. and Girbés, T. (2014) Probiotic Lactic Acid Bacteria: A Review. Food and Nutrition Sciences, 5, 1765-1775. doi: 10.4236/fns.2014.518190.

[1]   Hayek, S.A. and Ibrahim, S.A. (2013) Current Limitations and Challenges with Lactic Acid Bacteria: A Review. Food and Nutrition Sciences, 4, 73-87.

[2]   Orla-Jensen, S. (1919) The Latic Acid Bacteria. Andr. Fred. Host and Son, Copenhagen.

[3]   Von Wright, A. and Axelsson, L. (2012) Lactic Acid Bacteria: An Introduction. In: Lahtinen, S., Ouwehand, A.C., Salminen, S. and von Wright, A., Eds., Lactic Acid Bacteria: Microbiological and Functional Aspects, 4th Edition, Tayor & Francis Group LLC, CRC Press, Boca Raton, 1-16.

[4]   Anonymous (2014) NCBI Taxonomy Browser.

[5]   Anonymous (2014) UniProt Taxonomy browser.

[6]   Morelli, L., Calleagri, M.L., Vogensen, F.K. and von Wright, A. (2012) Genetics of Lactic Acid Bacteria. In: Lahtinen, S., Ouwehand, A.C., Salminen, S. and von Wright, A., Eds., Lactic Acid Bacteria: Microbiological and Functional Aspects, 4th Edition, Tayor & Francis Group LLC, CRC Press, Boca Raton, 17-38.

[7]   Hooper, L.V. and Macpherson, A.J. (2010) Immune Adaptations That Maintain Homeostasis with the Intestinal Microbiota. Nature Reviews Immunology, 10, 159-169.

[8]   Kamada, N., Seo, S.-U., Chen, G.Y. and Núñez, G. (2013) Role of the Gut Microbiota in Immunity and Inflammatory Disease. Nature Reviews Immunology, 13, 321-335.

[9]   Lebeer, S., Vanderleyden, J. and De Keersmaecker, S.C. (2008) Genes and Molecules of Lactobacilli Supporting Probiotic Action. Microbiology and Molecular Biology Reviews, 72, 728-764.

[10]   Klaenhammer, T.R., Barrangou, R., Buck, B.L., Azcarate-Peril, M.A. and Altermann, E. (2005) Genomic Features of Lactic Acid Bacteria Effecting Bioprocessing and Health. FEMS Microbiology Reviews, 29, 393-409.

[11]   Corfield, A.P., Myerscough, N., Longman, R., Sylvester, P., Arul, S. and Pignatelli, M. (2000) Mucins and Mucosal Protection in the Gastrointestinal Tract: New Prospects for Mucins in the Pathology of Gastrointestinal Disease. Gut, 47, 589-594.

[12]   Moal, V.L.-L. and Servin, A.L. (2006) The Front Line on Enteric Host Defence against Unwelcome Intrusion of Harmful Microorganisms: Mucins, Antimicrobial Peptides and Microbiota. Clinical Microbiology Reviews, 19, 315- 337.

[13]   Mack, D.R., Michail, S., Wei, S., McDougall, L. and Hollingsworth, M.A. (1999) Probiotics Inhibit Enteropathogenic E. coli Adherence in Vitro by Inducing Intestinal Mucin Gene Expression. American Journal of Physiology, 276, G941-G950.

[14]   Mack, D.R., Ahrne, S., Hyde, L., Wei, S. and Hollingsworth, M.A. (2003) Extracellular MUC3 Mucin Secretion Follows Adherence of Lactobacillus Strains to Intestinal Epithelial Cells in Vitro. Gut, 52, 827-833.

[15]   Servin, A.L. (2004) Antagonistic Activities of Lactobacilli and Bifidobacteria against Microbial Pathogens. FEMS Microbiology Reviews, 28, 405-440.

[16]   Bernet-Camard, M.F., Lievin, V., Brassart, D., Neeser, J.R., Servin, A.L. and Hudault, S. (1997) The Human Lactobacillus acidophilus Strain LA1 Secretes a Nonbacteriocin Antibacterial Substance(s) Active in Vitro and in Vivo. Applied and Environmental Microbiology, 63, 2747-2753.

[17]   Coconier, M.H., Bernet, M.F., Kerneis, S., Chauviere, G., Fourniat, J. and Sercin, A.L. (1993) Inhibition of Adhesion of Enteroinvasive Pathogens to Human Intestinal Caco-2 Cells by Lactobacillus acidophilus Strain LB Decreases Bacterial Invasion. FEMS Microbiology Letters, 110, 299-305.

[18]   Coconnier, M.H., Lievin, V., Bernet-Camard, M.F., Hudault, S. and Servin, A.L. (1997) Antibacterial Effect of the Adhering Human Lactobacillus acidophilus Strain LB. Antimicrobial Agents and Chemotherapy, 41, 1046-1052.

[19]   Coconnier, M.H., Lievin, V., Lorrot, M. and Servin, A.L. (2000) Antagonistic Activity of Lactobacillus acidophilus LB against Intracellular Salmonella enterica Serovar Typhimurium Infecting Human Enterocyte-Like Caco-2/TC-7 Cells. Applied and Environmental Microbiology, 66, 1152-1157.

[20]   Fayol-Messaoudi, D., Berger, C.N., Coconnier-Polter, M.-H., Moal, V.L.-L. and Servin, A.L. (2005) pH-, Lactic Acid- and Non-Lactic Acid-Dependent Activities of Probiotic Lactobacilli against Salmonella enterica Serovar Typhimurium. Applied and Environmental Microbiology, 71, 6008-6013.

[21]   Gopal, P.K., Prasad, J., Smart, J. and Gill, H.S. (2001) In Vitro Adherence Properties of Lactobacillus rhamnosus DR20 and Bifidobacterium lactis DR10 Strains and Their Antagonistic Activity against an Enterotoxigenic Escherichia coli. International Journal of Food Microbiology, 67, 207-216.

[22]   Hudault, S., Lievin, V., Bernet-Camard, M.F. and Servin, A.L. (1997) Antagonistic Activity Exerted in Vitro and in Vivo by Lactobacillus casei (Strain GG) against Salmonella typhimurium C5 Infection. Applied and Environmental Microbiology, 63, 513-518.

[23]   Altenhoefer, A., Oswald, S., Sonnenborn, U., Enders, C., Schulze, J., Hacker, J. and Oelschlaeger, T.A. (2004) The Probiotic Escherichia coli Strain Nissle 1917 Interferes with Invasion of Human Intestinal Epithelial Cells by Different Enteroinvasive Bacterial Pathogens. FEMS Immunology and Medical Microbiology, 40, 223-229.

[24]   Boudeau, J., Glasser, A.L., Julien, S., Colombel, J.F. and Darfeuille-Michaud, A. (2003) Inhibitory Effect of Probiotic Escherichia coli Strain Nissle 1917 on Adhesion to and Invasion of Intestinal Epithelial Cells by Adherentinvasive E. coli Strains Isolated from Patients with Crohn’s Disease. Alimentary Pharmacology & Therapeutics, 18, 45-56.

[25]   Montville, T.J. and Winkowski, K. (1997) Biologically Based Preservation Systems and Probiotic Bacteria. In: Doyle, M.P., Beuchat, L.R. and Montville, T.J., Eds., Food Microbiology: Fundamentals and Frontiers, ASM, Washington DC.

[26]   Hoover, G. and Steenson, L.R. (1993) Bacteriocins of Lactic Acid Bacteria. Academic Press Inc., New York.

[27]   Klaenhammer, T.R. (1993) Genetics of Bacteriocins Produced by Lactic Acid Bacteria. FEMS Microbiology Reviews, 12, 39-86.

[28]   Yang, S.-C., Lin, C.-H., Sung, C.T. and Fang, J.-Y. (2014) Antibacterial Activities of Bacteriocins: Application in Foods and Pharmaceuticals. Frontiers in Microbiology, 5, 1-10.

[29]   Cotter, P.D., Ross, R.P. and Hill, C. (2013) Bacteriocins—A Viable Alternative to Antibiotics? Nature Reviews Microbiology, 11, 95-105.

[30]   Hurst, A. (1981) Nisin. Advances in Applied Microbiology, 27, 85-123.

[31]   Cleveland, J., Montville, T.J., Nes, I.F. and Chikindas, M.L. (2001) Bacteriocins: Safe, Natural Antimicrobials for Food Preservation. International Journal of Food Microbiology, 71, 1-20.

[32]   Bernbom, N., Licht, T.R., Brogren, C.H., Jelle, B., Johansen, A.H., Badiola, I., Vogensen, F.K. and Norrung, B. (2006) Effects of Lactococcus lactis on Composition of Intestinal Microbiota: Role of Nisin. Applied and Environmental Microbiology, 72, 239-244.

[33]   Deitch, E., Specian, E., Steffen, E. and Berg, R. (1990) Translocation of Lactobacillus murinus from the Gastrointestinal Tract. Current Microbiology, 20, 177-184.

[34]   Atuma, C., Strugala, V., Allen, A. and Holm, L. (2001) The Adherent Gastrointestinal Mucus Gel Layer: Thickness and Physical State in Vivo. American Journal Physiology Gastrointestinal Liver Physiology, 280, G922-G929.

[35]   Johansson, M.E., Phillipson, M., Petersson, J., Velcich, A., Holm, L. and Hansson, G.C. (2008) The Inner of the Two Muc2 Mucin-Dependent Mucus Layers in Colon Is Devoid of Bacteria. Proceedings of the National Academy of Sciences of the United States of America, 105, 15064-15069.

[36]   Troost, F.J., van Baarlen, P., Lindsey, P., Kodde, A., de Vos, W.M., Kleerebezem, M. and Brummer, R.J. (2008) Identification of the Transcriptional Response of Human Intestinal Mucosa to Lactobacillus plantarum WCFS1 in Vivo. BMC Genomics, 9, 374.

[37]   van Baarlen, P., Troost, F.J., van Hemert, S., van der Meer, C., de Vos, W.M., de Groot, P.J., Hooiveld, G.J., Brummer, R.J. and Kleerebezem, M. (2009) Differential NF-KappaB Pathways Induction by Lactobacillus plantarum in the Duodenum of Healthy Humans Correlating with Immune Tolerance. Proceedings of the National Academy of Sciences of the United States of America, 106, 2371-2376.

[38]   Wells, J.M. (2011) Immunomodulatory Mechanisms of Lactobacilli. Microbial Cell Factories, 10, S17.

[39]   Coombes, J.L. and Maloy, K.J. (2007) Control of Intestinal Homeostasis by Regulatory T Cells and Dendritic Cells. Seminars in Immunology, 19, 116-126.

[40]   Bogunovic, M., Ginhoux, F., Helft, J., Shang, L., Hashimoto, D., Greter, M., Liu, K., Jakubzick, C., Ingersoll, M.A., Leboeuf, M., Stanley, E.R., Nussenzweig, M., Lira, S.A., Randolph, G.J., Merad, M. (2009) Origin of the Lamina Propria Dendritic Cell Network. Immunity, 31, 513-525.

[41]   Varol, C., Vallon-Eberhard, A., Elinav, E., Aychek, T., Shapira, Y., Luche, H., Fehling, H.J., Hardt, W.D., Shakhar, G. and Jung, S. (2009) Intestinal Lamina Propria Dendritic Cell Subsets Have Different Origin and Functions. Immunity, 31, 502-512.

[42]   Kwon, H.K., Lee, C.G., So, J.S., Chae, C.S., Hwang, J.S., Sahoo, A., Nam, J.H., Rhee, J.H., Hwang, K.C. and Im, S.H. (2010) Generation of Regulatory Dendritic Cells and CD4+Foxp3+ T Cells by Probiotics Administration Suppresses Immune Disorders. Proceedings of the National Academy of Sciences of the United States of America, 107, 2159-2164.

[43]   Pretzer, G., Snel, J., Molenaar, D., Wiersma, A., Bron, P.A., Lambert, J., de Vos, W.M., van der Meer, R., Smits, M.A. and Kleerebezem, M. (2005) Biodiversity-Based Identification and Functional Characterization of the Mannose-Specific Adhesin of Lactobacillus plantarum. Journal of Bacteriology, 187, 6128-6136.

[44]   Denou, E., Pridmore, R.D., Berger, B., Panoff, J.M., Arigoni, F. and Brussow, H. (2008) Identification of Genes Associated with the Long-Gut-Persistence Phenotype of the Probiotic Lactobacillus johnsonii Strain NCC533 Using a Combination of Genomics and Transcriptome Analysis. Journal of Bacteriology, 190, 3161-3168.

[45]   Meijerink, M., van Hemert, S., Taverne, N., Wels, M., de Vos, P., Bron, P.A., Savelkoul, H.F., van Bilsen, J., Kleerebezem, M. and Wells, J.M. (2010) Identification of Genetic Loci in Lactobacillus plantarum That Modulate the Immune Response of Dendritic Cells Using Comparative Genome Hybridization. PLoS ONE, 5, e10632.

[46]   Lakhdari, O., Cultrone, A., Tap, J., Gloux, K., Bernard, F., Ehrlich, S.D., Lefevre, F., Dore, J. and Blottiere, H.M. (2010) Functional Metagenomics: A High Throughput Screening Method to Decipher Microbiota-Driven NF-kappaB Modulation in the Human Gut. PLoS ONE, 5, e13092.

[47]   Meijerink, M. and Wells, J.M. (2010) Probiotic Modulation of Dendritic Cells and T Cell Responses in the Intestine. Beneficial Microbes, 1, 317-216.

[48]   Sazawal, S., Hiremath, G., Dhingra, U., Malik, P., Deb, S. and Black, R.E. (2006) Efficacy of Probiotics in Prevention of Acute Diarrhoea: A Meta-Analysis of Masked, Randomised, Placebo-Controlled Trials. Lancet Infectious Diseases, 6, 374-382.

[49]   Kalliomaki, M., Salminen, S., Arvilommi, H., Kero, P., Koskinen, P. and Isolauri, E. (2001) Probiotics in Primary Prevention of Atopic Disease: A Randomised Placebo-Controlled Trial. The Lancet, 357, 1076-1079.

[50]   Kalliomaki, M., Salminen, S., Poussa, T., Arvilommi, H. and Isolauri, E. (2003) Probiotics and Prevention of Atopic Disease: 4-Year Follow-Up of a Randomised Placebo-Controlled Trial. The Lancet, 361, 1869-1871.

[51]   Isolauri, E., Joensuu, J., Suomalainen, H., Luomala, M. and Vesikari, T. (1995) Improved Immunogenicity of Oral D x RRV Reassortant Rotavirus Vaccine by Lactobacillus casei GG. Vaccine, 13, 310-312.

[52]   de Vrese, M., Rautenberg, P., Laue, C., Koopmans, M., Herremans, T. and Schrezenmeir, J. (2005) Probiotic Bacteria Stimulate Virus-Specific Neutralizing Antibodies Following a Booster Polio Vaccination. European Journal of Nutrition, 44, 406-413.

[53]   Paineau, D., Carcano, D., Leyer, G., Darquy, S., Alyanakian, M.A., Simoneau, G., Bergmann, J.F., Brassart, D., Bornet, F. and Ouwehand, A.C. (2008) Effects of Seven Potential Probiotic Strains on Specific Immune Responses in Healthy Adults: A Double-Blind, Randomized, Controlled Trial. FEMS Immunology and Medical Microbiology, 53, 107-113.

[54]   Kobayashi, N., Saito, T., Uematsu, T., Kishi, K., Toba, M., Kohda, N. and Suzuki, T. (2011) Oral Administration of Heat-Killed Lactobacillus pentosus Strain b240 Augments Protection against Influenza Virus Infection in Mice. International Immunopharmacology, 11, 199-203.

[55]   Corcoran, B.M., Ross, R.P., Fitzgerald, G.F., Dockery, P. and Stanton, C. (2006) Enhanced Survival of GroESL-Overproducing Lactobacillus paracasei NFBC 338 under Stressful Conditions Induced by Drying. Applied and Environmental Microbiology, 72, 5104-5107.

[56]   Savage, D. (1977) Microbiology of the Gastrointestinal Tract. Annual Review of Microbiology, 31, 107-133.

[57]   McCraken, V.J. and Lorenz, R.G. (2001) The Gastrointestinal Ecosystem: Aprecarious Alliance among Epithelium, Immunity and Microbiota. Cellular Microbiology, 3, 1-11.

[58]   Simon, G.L. and Gorbach, S.L. (1984) Intestinal Flora in Health and Disease. Gastroenterology, 86, 174-193.

[59]   Mills, S., Stanton, C., Fitzgerald, G.F. and Ross, R.P. (2011) Enhancing the Stress Responses of Probiotics for a Lifestyle from Gut to Product and Back Again. Microbial Cell Factories, 10, S19.

[60]   Dethlefsen, L., Eckburg, P.B., Bik, E.M. and Relman, D.A. (2006) Assembly of the Human Intestinal Microbiota. Trends in Ecology & Evolution, 21, 517-523.

[61]   Montville, T.J. and Matthews, K. (2005) Food Microbiology: An Introduction. ASM Press, Washington DC.

[62]   Arvola, T., Laiho, K., Torkkeli, S., Mykkanen, H., Salminen, S., Maunula, L. and Isolauri, E. (1999) Prophylactic Lactobacillus GG Reduces Antibiotic-Associated Diarrhea in Children with Respiratory Infections: A Randomized Study. Pediatrics, 104, e64.

[63]   Turcanu, V. and Lack, G. (2006) Molecular and Immunological Responses to Food. In: Maleki, S.J., Burks, A.W. and Helm, R.M., Eds., Food Allergy, ASM Press, Washington DC, 83-121.

[64]   Ebel, B., Lemetais, G., Beney, L., Cachon, R., Sokol, H., Langella, P. and Gervais, P. (2014) Impact of Probiotics on Risk Factors for Cardiovascular Diseases. A Review. Critical Reviews in Food Science and Nutrition, 54, 175-189.

[65]   Ishikawa, H., Akedo, I., Umesaki, Y., Tanaka, R., Imaoka, A. and Otani, T. (2003) Randomized Controlled Trial of the Effect of Bifidobacteria-Fermented Milk on Ulcerative Colitis. Journal of the American College of Nutrition, 22, 56-63.

[66]   Kruis, W., Fric, P., Pokrotnieks, J., Lukas, M., Fixa, B., Kascak, M., Kamm, M.A., Weismuller, J., Beglinger, C., Stolte, M., Wolff, C. and Schulze, J. (2004) Maintaining Remission of Ulcerative Colitis with the Probiotic Escherichia coli Nissle 1917 Is as Effective as with Standard Mesalazine. Gut, 53, 1617-1623.

[67]   McFarland, L.V., Surawicz, C.M., Greenberg, R.N., Fekety, R., Elmer, G.W., Moyer, K.A., Melcher, S.A., Bowen, K.E., Cox, J.L., Noorani, Z., Harrington, G., Rubin, M. and Greenwald, D. (1994) A Randomized Placebo-Controlled Trial of Saccharomyces boulardii in Combination with Standard Antibiotics for Clostridium difficile Disease. JAMA, 271, 1913-1918.

[68]   Nista, E.C., Candelli, M., Cremonini, F., Cazzato, I.A., Zocco, M.A., Franceschi, F., Cammarota, G., Gasbarrini, G. and Gasbarrini, A. (2004) Bacillus clausii Therapy to Reduce Side-Effects of Anti-Helicobacter pylori Treatment: Randomized, Double-Blind, Placebo Controlled Trial. Alimentary Pharmacology & Therapeutics, 20, 1181-1188.

[69]   Wang, Y.W. and Jones, P.J.H. (2004) Conjugated Linoleic Acid and Obesity Control: Efficacy and Mechanisms. International Journal of Obesity and Related Metabolic Disorders, 28, 941-955.

[70]   Szajewska, H., Ruszczyn’ski, M. and Radzikowski, A. (2006) Probiotics in the Prevention of Antibiotic-Associated Diarrhea in Children: A Meta-Analysis of Randomized Controlled Trials. Journal of Pediatrics, 149, 367-372.

[71]   de Vrese, M., Stegelmann, A., Richter, B., Fenselau, S., Laue, C. and Schrezenmeir, J. (2001) Probiotics—Compensation for Lactase Insufficiency. American Journal of Clinical Nutrition, 73, 421S-429S.

[72]   Lucas, A. and Cole, T.J. (1990) Breast Milk and Neonatal Necrotising Enterocolitis. The Lancet, 336, 1519-1523.

[73]   Gewolb, I.H., Schwalbe, R.S., Taciak, V.L., Harrison, T.S. and Panigrahi, P. (1999) Stool Microflora in Extremely Low Birthweight Infants. Archives of Disease in Childhood Fetal Neonatal, 80, F167-F173.

[74]   Reid, G., Jass, J., Sebulsky, M.T. and McCormick, J.K. (2003) Potential Uses of Probiotics in Clinical Practice. Clinical Microbiology Reviews, 16, 658-672.

[75]   Hoyos, A.B. (1999) Reduced Incidence of Necrotizing Enterocolitis Associated with Enteral Administration of Lactobacillus acidophilus and Bifidobacterium infantis to Neonates in an Intensive Care Unit. International Journal of Infectious Diseases, 3, 197-202.

[76]   Caplan, M.S. and Jilling, T. (2000) Neonatal Necrotizing Enterocolitis: Possible Role of Probiotic Supplementation. Journal of Pediatric Gastroenterology and Nutrition, 30, S18-S22.

[77]   Rohan, T. and Wainwright, L. (2014) Does Administering Probiotic Treatment to Infants under 1500 g Decrease the Incidence of Necrotising Enterocolitis? A Systematic Literature Review. Journal of Neonatal Nursing, 20, 37-42.

[78]   Korterink, J.J., Ockeloen, L., Benninga, M.A., Tabbers, M.M., Hilbink, M. and Deckers-Kocken, J.M. (2014) Probiotics for Childhood Functional Gastrointestinal Disorders: A Systematic Review and Meta-Analysis. Acta Paediatrica, 103, 365-372.

[79]   de Silva, D., Geromi, M., Halken, S., Host, A., Panesar, S.S., Muraro, A., Werfel, T., Hoffmann-Sommergruber, K., Roberts, G., Cardona, V., Dubois, A.E.J., Poulsen, L.K., Van Ree, R., Vlieg-Boerstra, B., Agache, I., Grimshaw, K., O’Mahony, L., Venter, C., Arshad, S.H. and Sheikh, A., on Behalf of the EAACI Food Allergy and Anaphylaxis Guidelines Group (2014) Primary Prevention of Food Allergy in Children and Adults: Systematic Review. Allergy, 69, 581-589.

[80]   Redman, M.G., Ward, E.J. and Phillips, R.S. (2014) The Efficacy and Safety of Probiotics in People with Cancer: A Systematic Review. Annals of Oncology, 1-11.

[81]   Kunz, A.N., Noel, J.M. and Fairchok, M.P. (2004) Two Cases of Lactobacillus Bacteremia during Probiotic Treatment of Short Gut Syndrome. Journal of Pediatric Gastroenterology and Nutrition, 38, 457-458.

[82]   Land, M.H., Rouster-Stevens, K., Woods, C.R., Cannon, M.L., Cnota, J. and Shetty, A.K. (2005) Lactobacillus Sepsis Associated with Probiotic Therapy. Pediatrics, 115, 178-181.

[83]   National Cancer Institute (2010) Common Terminology Criteria for Adverse Events, Version 4.0.

[84]   Bermúdez-Humarán, L.G., Kharrat, P., Chatel, J.-M. and Langella, P. (2011) Lactococci and Lactobacilli as Mucosal Delivery Vectors for Therapeutic Proteins and DNA Vaccines. Microbial Cell Factories, 10, S4.

[85]   Davis, C. (2014) Enumeration of Probiotic Strains: Review of Culture-Dependent and Alternative Techniques to Quantify Viable Bacteria. Journal of Microbiological Methods, 103, 9-17.

[86]   Brooker, B.E. (1995) Imaging Food Systems by Confocal Scanning Laser Microscopy. In: Dickinson, E., Ed., New Physico-Chemical Techniques for the Characterization of Complex Food Systems, Blackie Academic and Profession, London, 43-68.

[87]   Heertje, I., van der Vlist, P., Blonk, J.C.G., Hendrickx, H.A.C. and Brackenhof, G.J. (1987) Confocal Scanning Laser Microscopy in Food Research: Some Observations. Food Microstructure, 6, 115-120.

[88]   Auty, M.A.E., Gardinere, G.C., Mc Breaty, S.J., O’Sullivan, E.O., Muerill, D.M., Collins, J.K., Fitzgerald, G.J., Stanton, C. and Ross, R.P. (2001) Direct in Situ Viability Assessment of Bacteria in Probiotic Dairy Products Using Viability Staining in Conjunction with Confocal Scanning Laser Microscopy. Applied and Environmental Microbiology, 67, 420-425.

[89]   Villlarreal, M.L.M., Padiha, M., Vieira, A.D.S., de Melo Franco, B.D.G., Martinez, R.C.R. and Saad, S.M.I. (2013) Advantageous Direct Quantification of Viable Closely Related Probiotics in Petit-Suisse Cheeses under in Vitro Gastrointestinal Conditions by Propidium Monoazide-qPCR. PLoS ONE, 8, e82102.

[90]   Nogva, H.K., Dr?mtorp, S.M., Nissen, H. and Rudi, K. (2003) Ethidium Monoazide for DNA-Based Differentiation of Viable and Dead Bacteria by 5'-Nuclease PCR. BioTechniques, 34, 804-808, 810, 812-813.

[91]   Fittipaldi, M., Nocker, A. and Codony, F. (2012) Progress in Understanding Preferential Detection of Live Cells Using Viability Dyes in Combination with DNA Amplification. Journal of Microbiolgical Methods, 91, 276-289.

[92]   Chen, S., Cao, Y., Ferguson, L.R., Shu, Q. and Garg, S. (2012) Flow Cytometric Assessment of the Protectants for Enhanced in Vitro Survival of Probiotic Lactic Acid Bacteria through Simulated Human Gastro-Intestinal Stresses. Applied Microbiology and Biotechnology, 95, 345-356.

[93]   FAO/WHO (2002) Joint FAO/WHO Working Group Report on Drafting Guidelines for the Evaluation of Probiotics in Food. London, Ontario, Canada, April 30 and May 1.

[94]   Tenover, F.C. (2006) Mechanisms of Antimicrobial Resistance in Bacteria. American Journal of Medicine, 119, S3- S10.

[95]   D’Aimmo, M.R., Modesto, M. and Biavati, B. (2007) Antibiotic Resistance of Lactic Acid Bacteria and Bifidobacterium Spp. Isolated from Dairy and Pharmaceutical Products. International Journal of Food Microbiology, 115, 35-42.

[96]   Fukao, M. and Yajima, N. (2012) Assessment of Antibiotic Resistance in Probiotic Lactobacilli. In: Pana, M., Ed., Antibiotic Resistant Bacteria—A Continuous Challenge in the New Millekium, InTech.

[97]   Patel, A.R., Shah, N.P. and Prajapati, J.B. (2012) Antibiotic Resistance Profile of Lactic Acid Bacteria and Their Implication in Food Chain. World Journal of Dairy & Food Sciences, 7, 202-211.

[98]   Flórez, A.B., Danielsen, M., Korhonen, J., Zyckal, J., von Wright, A., Bardowski, J. and Mayo, B. (2007) Antibiotic Survey of Lactococcus lactis Strains to Six Antibiotics by Etest and Establishment of New Susceptibility-Resistance Cut-Off Values. Journal of Dairy Research, 74, 262-268.

[99]   Ammor, M.S., Flórez, A.B., van Hoek, A.H., de Los Reyes-Gavilán, C.G., Aarts, H.J., Margolles, A. and Mayo, B. (2008) Molecular Characterization of Intrinsic and Acquired Antibiotic Resistance in Lactic Acid Bacteria and Bifidobacteria. Journal of Molecular Microbiology and Biotechnology, 14, 6-15.

[100]   Klare, I., Konstabel, C., Werner, G., Huys, G., Vankerckhoven, V., Kahlmeter, G., Hildebrandt, B., Müller-Bertling, S., Witte, W. and Goossens, H. (2007) Antimicrobial Susceptibilities of Lactobacillus, Pedioccoccus and Lactococcus Human Isolates and Cultures Intended for Probiotic or Nutritional Use. Journal of Antimicrobial Chemotherapy, 59, 900-912.

[101]   Korhonen, J.M., Van Hoek, A.H., Saarela, M., Huys, G., Tosi, L., Mayrhofer, S. and Wright, A.V. (2010) Antimicrobial Susceptibility of Lactobacillus rhamnosus. Beneficial Microbes, 1, 75-80.

[102]   Salminen, S., Ouwenhand, A., Benno, Y. and Lee, Y.K. (1999) Probiotics: How Should They Be Defined? Trends in Food Science and Technology, 10, 107-110.

[103]   Chikindas, M.L. (2014) Probiotics and Antimicrobial Peptides: The Creatures’ and Substances’ Future in the Twenty-First Century: An Opinion Letter. Probiotics and Antimicrobial Proteins, 6, 69-72.

[104]   Besselink, M.G., van Santvoort, H.C., Buskens, E., Boermeester, M.A., van Goor, H., Timmerman, H.M., Nieuwenhuijs, V.B., Bollen, T.L., van Ramshorst, B., Witterman, B.J.M., Rosman, C., Ploeg, R.J., Brink, M.A., Schaapherder, A.F.M., Dejong, C.H.C., Wahab, P.J., van Laarhoven, C.J.H.M., van der Harst, E., van Eijck, C.H.J., Cuesta, M.A., Akkermans, L.M.A. and Gooszen, H.G. (2008) Probiotic Prophylaxis in Predicted Severe Acute Pancreatitis: A Randomised, Double-Blind, Placebo-Controlled Trial. The Lancet, 371, 651-659.