AiM  Vol.4 No.12 , September 2014
The Evaluation of Bioremediation Potential of a Yeast Collection Isolated from Composting
ABSTRACT
The influence of xenobiotic compounds on environment and on living organisms has been reported as an imminent public health problem. Among them we can list the contamination by Alkanes present in petroleum, hydrocarbons and organic contaminant substances from industrial effluents. Also, heavy metals are of particular interest because of their persistence in the environment contaminating the food webs. Among the innovative solutions for treatment of contaminated water and soil is the use of biological materials like living or dead microorganisms. Yeasts exhibit the ability to adapt to extreme condition such as temperature, pH and levels of organic and inorganic contaminants that make them a potential material to be used to remediate contaminated environment application. The goal of this work was to search for yeast isolates capable to use n-hexadecane (alkane hydrocarbon) as a primary carbon source and for those able to tolerate high concentration of lead (Pb) within a collection of 90 isolates obtained from the Sao Paulo Zoo composting system. The isolated yeast strains were identified by mass spectrometry (MALDI-TOF-MS) and by sequencing of the ribosomal DNA (18S and D1/D2) conserved regions. We found that the collection bares 23 isolates capable of utilizing n-hexadecane and one which is able to tolerate high concentration of lead (Pb) with a high biosorption index compared to the reference yeast strains (BY4742, PE-2, CAT-1 and BG-1). These results confirm the initial hypothesis that the Sao Paulo Zoo composting is the source for diverse yeasts species with biotechnological application potential.

Cite this paper
Trama, B. , Fernandes, J. , Labuto, G. , Oliveira, J. , Viana-Niero, C. , Pascon, R. and Vallim, M. (2014) The Evaluation of Bioremediation Potential of a Yeast Collection Isolated from Composting. Advances in Microbiology, 4, 796-807. doi: 10.4236/aim.2014.412088.
References
[1]   Fleishman, E., Blockstein, D.E., Hall, J.A., et al. (2011) Top 40 Priorities for Science to Inform US Conservation and Management Policy. BioScience, 61, 290-300.
http://dx.doi.org/10.1525/bio.2011.61.4.9

[2]   Zhang, Z., Hou, Z., Yang, C., et al. (2010) Degradation of n-Alkanes and Polycyclic Aromatic Hydrocarbons in Petroleum by a Newly Isolated Pseudomonas aeruginosa DQ8. Bioresource Technology, 102, 4111-4116. http://dx.doi.org/10.1016/j.biortech.2010.12.064

[3]   Wang, J.L. and Chen, C. (2006) Biosorption of Heavy Metals by Saccharomyces cerevisiae: A Review. Biotechnology Advances, 24, 427-451. http://dx.doi.org/10.1016/j.biotechadv.2006.03.001

[4]   Lessler, M.A. (1988) Lead and Lead Poisoning from Antiquity to Modern Times. The Ohio Journal of Science, 88, 78-84.

[5]   Demayo, A., Taylor, C.M., Taylor, K.W., et al. (1982) Toxic Effects of Lead and Lead Compounds on Human Health, Aquatic Life, Wildlife Plants, and Livestock. C R C Critical Reviews in Environmental Control, 12, 257-305. http://dx.doi.org/10.1080/10643388209381698

[6]   Moreira, F.R. and Moreira, J.C. (2004) Os efeitos do chumbo sobre o organismo humano e seu significado para a saúde. Revista Panamericana de Salud Pública, 15, 119-129.
http://dx.doi.org/10.1590/S1020-49892004000200007

[7]   Cui, J. and Zhang, L. (2008) Metallurgical Recovery of Metals from Electronic Waste: A Review. Journal of Hazardous Materials, 158, 228-256. http://dx.doi.org/10.1016/j.jhazmat.2008.02.001

[8]   Song, H., Lia, X., Suna, J., Xua, S. and Hanb, X. (2008) Application of a Magnetotactic Bacterium, Stenotrophomonas sp. to the removal of Au(III) from Contaminated Wastewater with a Magnetic Separator. Chemosphere, 72, 616-621. http://dx.doi.org/10.1016/j.chemosphere.2008.02.064

[9]   Wang, J.L. and Chen, C. (2009) Biosorbents for Heavy Metals Removal and Their Future. Biotechnology Advances, 27, 195-226. http://dx.doi.org/10.1016/j.biotechadv.2008.11.002

[10]   Kolvenbach, B.A., Helbling, D.E., Kohler, H.P. and Corvini, P.F. (2014) Emerging Chemicals and the Evolution of Biodegradation Capacities and Pathways in Bacteria. Current Opinion in Biotechnology, 27C, 8-14. http://dx.doi.org/10.1016/j.copbio.2013.08.017

[11]   Kumar, A., Bisht, B.S., Joshi, V.D. and Dhewa, T. (2011) Review on Bioremediation of Polluted Environment: A Management Tool. International Journal of Environmental Sciences, 1, 1079-1093.

[12]   McGenity, T.J. (2014) Hydrocarbon Biodegradation in Intertidal Wetland Sediments. Current Opinion in Biotechnology, 27, 46-54. http://dx.doi.org/10.1016/j.copbio.2013.10.010

[13]   Dursun, A.Y., Uslua, G., Cuci, Y. and Aksub, Z. (2003) Bioaccumulation of Copper(II), Lead(II) and Chromium(VI) by Growing Aspergillus niger. Process Biochemistry, 38, 1647-1651.
http://dx.doi.org/10.1016/S0032-9592(02)00075-4

[14]   Li, C., Jiang, W., Ma, N., et al. (2014) Bioaccumulation of Cadmium by Growing Zygosaccharomyces rouxii and Saccharomyces cerevisiae. Bioresource Technology, 155, 116-121.
http://dx.doi.org/10.1016/j.biortech.2013.12.098

[15]   Joo, H.S., Ndegwa, P.M., Shoda, M. and Phae, C.G. (2008) Bioremediation of Oil-Contaminated Soil Using Candida catenulata and Food Waste. Environmental Pollution, 156, 891-896.
http://dx.doi.org/10.1016/j.envpol.2008.05.026

[16]   Karigar, C.H. and Rao, S.S. (2011) Role of Microbial Enzymes in the Bioremediation of Pollutants: A Review. Enzyme Research, 2011, Article ID: 805187. http://dx.doi.org/10.4061/2011/805187

[17]   Munoz, A.J., Ruiz, E. and Abriouel, H. (2012) Heavy Metal Tolerance of Microorganisms Isolated from Wastewaters: Identification and Evaluation of Its Potential for Biosorption. Chemical Engineering Journal, 210, 325-332. http://dx.doi.org/10.1016/j.cej.2012.09.007

[18]   Watanabe, K., Kodoma, Y., Stutsubo, K. and Harayama, S. (2000) Molecular Characterization of Bacterial Populations in Petroleum-Contaminated Groundwater Discharged from Underground Crude Oil Storage Cavities. Applied and Environmental Microbiology, 66, 4803-4809.
http://dx.doi.org/10.1128/AEM.66.11.4803-4809.2000

[19]   Bitencourt, A.L.V., Vallim, M.A., Maia, D., et al. (2010) Core Sampling Test in Large-Scale Compost Cells for Microorganism Isolation. African Journal of Microbiology Research, 4, 1631-1634.

[20]   Dutra, E.S., Pascon, R.C. and Vallim, M.A. (2013) Sao Paulo Zoo Composting as a Source of Bacteria with Bioremediation Potential. African Journal of Microbiology Research, 7, 5200-5206.

[21]   Martins, L.F., Antunes, L.P., Pascon, R.C., et al. (2013) Correction: Metagenomic Analysis of a Tropical Composting Operation at the Sao Paulo Zoo Park Reveals Diversity of Biomass Degradation Functions and Organisms. PLoS ONE, 8, Article ID: e61928.
http://dx.doi.org/10.1371/journal.pone.0061928

[22]   Pascon, R.C., Bergamo, R.F., Spinelli, R.X., et al. (2011) Amylolytic Microorganism from Sao Paulo Zoo Composting: Isolation, Identification and Amylase Production. Enzyme Research, 2011, Article ID: 679624. http://dx.doi.org/10.4061/2011/679624

[23]   Basso, L.C., De Amorim, H.V., De Oliveira, A.J. and Lopes, M.L. (2008) Yeast Selection for Fuel Ethanol Production in Brazil. FEMS Yeast Research, 8, 1155-1163. http://dx.doi.org/10.1111/j.1567-1364.2008.00428.x

[24]   Freiwald, A. and Sauer, S. (2009) Phylogenetic Classification and Identification of Bacteria by Mass Spectrometry. Nature Protocols, 4, 732-742. http://dx.doi.org/10.1038/nprot.2009.37

[25]   Hoffman, C.S. and Winston, F. (1987) A Ten-Minute Preparation from Yeast Efficiently Releases Autonomous Plasmids for Transformation of Escherichia coli. Gene, 57, 267-272.
http://dx.doi.org/10.1016/0378-1119(87)90131-4

[26]   Rabanal, R.M., Arias, A., Prado, B., Hernández-Pérez, M. and Sánchez-Mateo, C.C. (2002) Antimicrobial Studies on Three Species of Hypericum from the Canary Islands. Journal of Ethnopharmacology, 81, 287-292. http://dx.doi.org/10.1016/S0378-8741(02)00083-1

[27]   Darangwa, N., Katskov, D.A. and Heitmann, U. (2013) Making ETAAS Determination Less Dependent on Vaporization Kinetics of the Analytes. South African Journal of Chemistry, 66, 207-215.

[28]   Kricka, W., Fitzpatrick, J. and Bond, U. (2014) Metabolic Engineering of Yeasts by Heterologous Enzyme Production for Degradation of Cellulose and Hemicellulose from Biomass: A Perspective. Frontiers in Microbiology, 5, 174.

[29]   Nielsen, J., Larsson, C., van Maris, A. and Pronk, J. (2013) Metabolic Engineering of Yeast for Production of Fuels and Chemicals. Current Opinion in Biotechnology, 24, 398-404.
http://dx.doi.org/10.1016/j.copbio.2013.03.023

[30]   Blackwell, K.J., Singleton, I. and Tobin, J.M. (1995) Metal Cation Uptake by Yeast: A Review. Applied Microbiology and Biotechnology, 43, 579-584. http://dx.doi.org/10.1007/BF00164757

[31]   Das, N., Vimala, R. and Karthika, P. (2008) Biosorption of Heavy Metals—An Overview. Indian Journal of Biotechnology, 7, 159-169.

[32]   Kordialik-Bogacka, E. (2011) Surface Properties of Yeast Cells during Heavy Metal Biosorption. Central European Journal of Chemistry, 9, 348-351. http://dx.doi.org/10.2478/s11532-011-0008-8

[33]   Zoghi, A., Khosravi-Darani, K. and Sohrabvandi, S. (2014) Surface Binding of Toxins and Heavy Metals by Probiotics. Mini-Reviews in Medicinal Chemistry, 14, 84-98.
http://dx.doi.org/10.2174/1389557513666131211105554

[34]   Johnson, E.A. (2013) Biotechnology of Non-Saccharomyces Yeasts—The Basidiomycetes. Applied Microbiology and Biotechnology, 97, 7563-7577. http://dx.doi.org/10.1007/s00253-013-5046-z

[35]   Kirk, P.M., Cannon, P.F., David, J.C. and Stalpers, J. (2008) Ainsworth & Bisby’s Dictionary of the Fungi. CAB International, Wallingford.

[36]   Van der Wal, A., Geydan, T.D., Kuyper, T.W. and de Boer, W. (2013) A Thready Affair: Linking Fungal Diversity and Community Dynamics to Terrestrial Decomposition Processes. FEMS Microbiology Reviews, 37, 477-494. http://dx.doi.org/10.1111/1574-6976.12001

[37]   Berg, B. and McClaugherty, C. (2008) Plant Litter—Decomposition, Humus Formation, Carbon Sequestration. Springer-Verlag, Berlin, Heidelberg.

[38]   Kurtzman, C.P. and Fell, J.W. (2011) The Yeasts: A Taxonomic Study. Elsevier, Amsterdam.

[39]   Deivasigamani, C. and Das, N. (2011) Biodegradation of Basic Violet 3 by Candida krusei Isolated from Textile Wastewater. Biodegradation, 22, 1169-1180. http://dx.doi.org/10.1007/s10532-011-9472-2

[40]   Domínguez, M.P., Sánchez-Montero, J.M., Sinisterra, J.V. and Alcántara, A.R. (2006) Understanding Candida rugosa Lipases: An Overview. Biotechnology Advances, 24, 180-196.
http://dx.doi.org/10.1016/j.biotechadv.2005.09.003

[41]   Duarte, E.A.A., Lacerda Jr., G.V., de Oliveira, T.A.S., et al. (2013) Bioprospection of Bacteria and Yeasts from Atlantic Rainforest Soil Capable of Growing in Crude-Glycerol Residues. Genetics and Molecular Research, 12, 4422-4433. http://dx.doi.org/10.4238/2013.October.10.8

[42]   El-Latif, H.A., Khan, S., Liu, X., et al. (2006) Application of PCR-DGGE to Analyse the Yeast Population Dynamics in Slurry Reactors during Degradation of Polycyclic Aromatic Hydrocarbons in Weathered Oil. Yeast, 23, 879-887. http://dx.doi.org/10.1002/yea.1401

[43]   Romer, M.C., Hammer, E., Cazau, M.C. and Arambarri, A.M. (2002) Isolation and Characterization of Biarylic Structure-Degrading Yeasts: Hydroxylation Potential of Dibenzofuran. Environmental Pollution, 118, 379-382. http://dx.doi.org/10.1016/S0269-7491(01)00290-1

[44]   El-Batal, A.I. (2002) Continuous Production of L-Phenylalanine by Rhodotorula glutinis Immobilized Cells Using a Column Reactor. Acta Microbiologica Polonica, 51, 153-169.

[45]   Frengova, G.I. and Beshkova, D.M. (2008) Carotenoids from Rhodotorula and Phaffia: Yeasts of Biotechnological Importance. Journal of Industrial Microbiology & Biotechnology, 36, 163-180.
http://dx.doi.org/10.1007/s10295-008-0492-9

[46]   Hainal, A.R., Capraru, A.M., Volf, I. and Popa, V.I. (2012) Lignin as a Carbon Source for the Cultivation of Some Rhodotorula Species. Cellulose Chemistry and Technology, 46, 87-96.

[47]   Quinn, A.J., Pickup, M.J. and D’Cunha, G.B. (2011) Enzyme Activity Evaluation of Organic Solvent-Treated Phenylalanine Ammonia Lyase. Biotechnology Progress, 27, 1554-1560.
http://dx.doi.org/10.1002/btpr.687

[48]   Taskin, M. (2013) Co-Production of Tannase and Pectinase by Free and Immobilized Cells of the Yeast Rhodotorula glutinis MP-10 Isolated from Tannin-Rich Persimmon (Diospyros kaki L.) Fruits. Bioprocess and Biosystems Engineering, 36, 165-172. http://dx.doi.org/10.1007/s00449-012-0771-8

[49]   Ilhan, S., Cabuk, A., Filik, C. and Calikan, F. (2004) Effect of Pretreatment on Biosorption of Heavy Metals by Fungal Biomass. Trakya University Journal of Social Science, 5, 11-17.

[50]   Del Carratore, R., Croce, C.D., Simili, M., et al. (2002) Cell Cycle and Morphological Alterations as Indicative of Apoptosis Promoted by UV Irradiation in S. cerevisiae. Mutation Research, 513, 183-191. http://dx.doi.org/10.1016/S1383-5718(01)00310-2

[51]   Machado, M.D., Santos, M.S., Gouveia, C., Soares, H.M. and Soares, E.V. (2009) Removal of Heavy Metals Using a Brewer’s Yeast Strain of Saccharomyces cerevisiae: The Flocculation as a Separation Process. Bioresources Technology, 99, 2107-2115. http://dx.doi.org/10.1016/j.biortech.2007.05.047

[52]   Chojnacka, H. (2010) Biosorption and Bioaccumulation—The Prospects for Practical Applications. Environment International, 36, 299-307. http://dx.doi.org/10.1016/j.envint.2009.12.001

[53]   Valente, P., Ramos, J.P. and Leoncini, O. (1999) Sequencing as a Tool in Yeast Molecular Taxonomy. Canadian Journal of Microbiology, 45, 949-958. http://dx.doi.org/10.1139/w99-094

 
 
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