FNS  Vol.5 No.17 , September 2014
Usefulness of Natural Starters in Food Industry: The Example of Cheeses and Bread
Abstract: Natural starters have been extensively used for many centuries to make many different fermented food products from different raw materials: Milk, meat, roots, vegetables, etc. The industrialisation of food production at the end of the 19th century necessitated the use of regular selected starters to standardize the organoleptic characteristics of the final product. As a consequence, during the 20th century, there was a decline in the use of natural starters in Western countries except in the production of local cheeses or sourdough breads. The beginning of this new millennium has witnessed a deep change in consumer demand, in pursuit of quality, safety and pleasure. In this context, natural starters could, in the future, play an important role in the development of fermented products. However, food producers and researchers have first to cope with fundamental problems in the understanding of these complex ecosystems. The dynamic evolution of the microbial population inside the natural starter (its resilience, its genetic and physiological aptitudes) and the consequences on the product are still partially unknown. This document reviews a broad range of articles concerning the use of natural starters with a specific focus on cheeses and breads, and discusses the major stakes for local food production and the consumption of typical products.
Cite this paper: Yann, D. and Pauline, G. (2014) Usefulness of Natural Starters in Food Industry: The Example of Cheeses and Bread. Food and Nutrition Sciences, 5, 1679-1691. doi: 10.4236/fns.2014.517181.

[1]   Roger, C. (2013) La qualité et la sécurité sanitaire des produits alimentaires: Un des enjeux du Millénium Round de l’OMC. Internet Département Economie et Sociologie Rurales—INRA.

[2]   Demarigny, Y. (2013) Les écosystèmes microbiens fromagers. Ph.D. Dissertation, UCBL1, Lyon.

[3]   Scintu, M.F. and Piredda, G. (2007) Typicity and Biodiversity of Goat and Sheep Milk Products. Small Ruminant Research, 68, 221-231.

[4]   Holzapfel, W.H. (2002) Appropriate Starter Culture Technologies for Small-Scale Fermentation in Developing Countries. International Journal of Food Microbiology, 75, 197-212.

[5]   Hansen, E.B. (2002) Commercial Bacterial Starter Cultures for Fermented Foods of the Future. International Journal of Food Microbiology, 78, 119-131.

[6]   Demarigny, Y. (2012) Fermented Food Products Made with Vegetable Materials from Tropical and Warm Countries: Microbial and Technological Considerations. International Journal of Food Science and Technology, 47, 2469-2476.

[7]   Lonvaud-Funel, A. (2008) Du raisin au vin: L’activité d’un système microbien dynamique. Biofutur, 294, 26-29.

[8]   Leroy, F. and De Vuyst, L. (2004) Lactic Acid Bacteria as Functional Starter Cultures for the Food Fermentation Industry. Trends in Food Science and Technology, 15, 67-78.

[9]   Froc, J. (2005) Nomadisme et sédentarisation: De l’airag au soja natto, du sapsago au vin jaune. In: Montel, M.C., Béranger, C. and Bonnemaire, J., Eds., Les fermentations au service des produits de terroir, INRA Editions, Paris, 39-46.

[10]   Steinkraus, K.H. (2002) Fermentations in World Food Processing. Comprehensive Review in Food Science and Food Safety, 1, 23-32.

[11]   FAO (2011) Current Status and Options for Biotechnologies in Food Processing and in Food Safety in Developing Countries. In: Biotechnologies for Agricultural Development, Proceedings of the FAO International Technical Conference on “Agricultural Biotechnologies in Developing Countries: Options and Opportunities in Crops, Forestry, Livestock, Fisheries and Agro-Industry to Face the Challenges of Food Insecurity and Climate Change”, ABDC-10, 240-277.

[12]   Neuzil, E. and Devaux, G. (1999) Le koumys, hier et aujourd’hui. Bulletin de la Société de Pharmacie de Bordeaux, 138, 91-112.

[13]   Buckenhüskes, H.J. (1993) Selection Criteria for Lactic Acid Bacteria to Be Used as Starter Cultures for Various Food Commodities. Federation of European Microbiological Societies Microbiology Reviews, 12, 253-271.

[14]   Hwanhlem, N., Buradaleng, S., Wattanachant, S., Benjakul, S., Tani, A. and Maneerat, S. (2011) Isolation and Screening of Lactic Acid Bacteria from Thai Traditional Fermented Fish (Plasom) and Production of Plasom from Selected Strains. Food Control, 22, 401-407.

[15]   INPB (1996) Levains et Panification. Les Nouvelles de la Boulangerie Patisserie, Supplément Technique, 54.

[16]   Corsetti, A. and Settanni, L. (2007) Lactobacilli in Sourdough Fermentation. Food Research International, 40, 539-558.

[17]   Demarigny, Y., Sabatier, C., Laurent, N., Prestoz, S., Rigobello, V. and Blachier, M.J. (2006) Microbiological Diversity in Natural Whey Starters Used to Make Traditional Rocamadour Goat Cheese and Possible Relationships with Its Bitterness. Italian Journal of Food Science, 18, 261-276.

[18]   Bouton, Y., Guyot, P. and Grappin, G. (1998) Preliminary Characterization of Microflora of Comté Cheese. Journal of Applied Microbiology, 85, 123-131.

[19]   Rossetti, L., Fornasari, M.E., Gatti, M., Lazzi, C., Neviani, E. and Giraffa, G. (2008) Grana Padano Cheese Whey Starters: Microbial Composition and Strain Distribution. International Journal of Food Microbiology, 127, 168-171.

[20]   Coppola, S., Parente, E., Dumontet, S. and La Peccerella, A. (1988) The Microflora of Natural Whey Cultures Utilized as Starters in the Manufacture of Mozzarella Cheese from Water-Buffalo Milk. Lait, 68, 295-309.

[21]   Ercolini, D., Moschetti, G., Blaiotta, G. and Coppola, S. (2001) The Potential of a Polyphasic PCR-DGGE Approach in Evaluating Microbial Diversity of Natural Whey Cultures for Water-Buffalo Mozzarella Cheese Production: Bias of Culture-Dependent and Culture-Independent Analyses. Systematic and Applied Microbiology, 24, 610-617.

[22]   Baruzzi, F., Matarante, A., Morea, M. and Cocconcelli, P.S. (2002) Microbial Community Dynamics during the Scamorza Altamurana Cheese Natural Fermentation. Journal of Dairy Science, 85, 1390-1397.

[23]   Ercolini, D., Frisso, G., Mauriello, G., Salvatore, F. and Coppola, S. (2008) Microbial Diversity in Natural Whey Cultures Used for the Production of Caciocavallo Silano PDO Cheese. International Journal of Food Microbiology, 124, 164-170.

[24]   Laithier, C., Raynaud, S. and Bonnes, A. (2012) Contribuer à la performance technico-économique des exploitations fromagères fermières en améliorant la ma?trise technologique et la qualité des fromages. Innovations en Agronomie, 25, 269-282.

[25]   Dalmasso, M. (2009) Etude de l’influence du repiquage sur la complexité des lactosérums levains. Application aux modèles fromagers de type pate pressée non cuite et de type mixte à dominante lactique. Ph.D. Dissertation, Université de Chambéry, Savoy.

[26]   Baruzzi, F., Matarante, A., Morea, M. and Cocconcelli, P.S. (2002) Microbial Community Dynamics during the Scamorza Altamurana Cheese Natural Fermentation. Journal of Dairy Science, 85, 1390-1397.

[27]   Salvadori del Prato, O. (2002) Bacterial and Technological Diversity: Interaction. Congrilait, Paris.

[28]   Vrancken, G., Rimaux, T., Weckx, S., Leroy, F. and de Vuyst, L. (2011) Influence of Temperature and Backslopping Time on the Microbiota of a Type I Propagated Laboratory Wheat Sourdough Fermentation. Applied and Environmental Microbiology, 77, 2716-2726.

[29]   Zagorec, M., Champomier-Vergès, M., Renault, P., Valence, F., Le loir, Y. and Montel, M.C. (2012) Ecosystèmes microbiens et préservation des aliments. Innovations en Agronomie, 24, 57-77.

[30]   Dalmasso, M., Prestoz, S., Rigobello, V. and Demarigny, Y. (2008) Evolution of the Raw Cow Milk Microflora, Especially Lactococci, Enterococci, Leuconostocs and Lactobacilli over a Successive 12 Day Milking Regime. International Journal of Dairy Science, 3, 117-130.

[31]   Laithier, C., Chatelin, Y.M., Tormo, H. and Lefrileux, Y. (2004) Biofilms in Farms Producing Goat Cheese: Localisation, Nature and Role on Products Quality. In: Actes des 11ème Rencontres Recherche Ruminant, Paris, 11-12.

[32]   Vacheyrou, M., Normand, A.C., Guyot, P., Cassagne, C., Piarroux, R. and Bouton, Y. (2011) Cultivable Microbial Communities in Raw Cow Milk and Potential Transfers from Stables of Sixteen French Farms. International Journal of Food Microbiology, 146, 253-262.

[33]   Callon, C., Duthoit, F., Delbès, C., Ferrand, M., Le Frileux, Y., De Crémoux, R. and Montel, M.C. (2007) Stability of Microbial Communities in Goat Milk during a Lactation Year: Molecular Approaches. Systematic and Applied Microbiology, 30, 547-560.

[34]   Demarigny, Y., Dalmasso, M., Tonleu, A., Rigobello, V., Beuvier, E., Ly-Chatain, M.H. and Bouton, Y. (2011) Influence of the Backslopping Practice on the Microbial Diversity of the Lactococcus Population in a Model Cheesemaking. Food and Nutrition Sciences, 2, 618-627.

[35]   Desmasures, N. and Beuvier, E. (2011) Chapitre 1: Ce qu’il faut savoir avant d’intervenir sur les microflores des laits. I. Nature et quantité de microflores des laits. In: Microflores des laits, Réseau Fromages de terroir, Paris, 4-14.

[36]   Lazzi, C., Rossetti, L., Zago, M., Neviani, E. and Giraffa, G. (2004) Evaluation of Bacterial Communities Belonging to Natural Whey Starters for Grana Padano Cheese by Length Heterogeneity-PCR. Journal of Applied Microbiology, 96, 481-490.

[37]   Rossetti, L., Fornasari, M.E., Gatti, M., Lazzi, C., Neviani, E. and Giraffa, G. (2008) Grana Padano Cheese Whey Starters: Microbial Composition and Strain Distribution. International Journal of Food Microbiology, 127, 168-171.

[38]   Arendt, E.K., Ryan, L.A.M. and Dal Bello, F. (2007) Impact of Sourdough on the Texture of Bread. Food Microbiology, 24, 165-174.

[39]   Vera, A., Ly-Chatain, M.H., Rigobello, V. and Demarigny, Y. (2012) Description of a French Natural Wheat Sourdough over 10 Consecutive Days Focussing on the Lactobacilli Present in the Microbiota. Antonie van Leeuwenhoek, 101, 369-377.

[40]   Zambonelli, C., Chiavari, C. and Benevelli, M. (2003) Starter microbici naturali e selezionati per la produzione di alimenti fermentati. Industrie Alimentari, 42, 617-623.

[41]   Salama, M.S., Musafija-Jeknic, T., Sandine, W.E. and Giovannoni, S.J. (1995) An Ecological Study of Lactic Acid Bacteria: Isolation of New Strains of Lactococcus Including Lactococcus lactis Subspecies cremoris. Journal of Dairy Science, 78, 1004-1017.

[42]   Kelly, B.G., Vespermann, A. and Bolton, D.J. (2009) Gene Transfer Events and Their Occurrence in Selected Environments. Food and Chemical Toxicology, 47, 978-983.

[43]   Nata?li, M. and Briandet, R. (2013) Les cités microbiennes. Biofutur, 3, 24-27.

[44]   van de Guchte, M., Serror, P., Chervaux, C., Smokvina, T., Ehrlich, S.D. and Maguin, E. (2002) Stress Responses in Lactic Acid Bacteria. Antonie van Leeuwenhoek, 82, 187-216.

[45]   Heylighen, F. (2008) Complexity and Self-Organization. In: Encyclopedia of Librairy and Information Sciences, Taylor & Francis, London, 1-20.

[46]   Doré, J. and Corthier, G. (2010) Le Microbiote Intestinal. Gastroentérologie Clinique et Biologique, 34, 7-10.

[47]   Cretenet, M., Laroute, V., Ulvé, V., Jeanson, S., Nouaille, S., Even, S., Piot, M., Girbal, L., Le Loir, Y., Loubière, P., Lortal, S. and Cocaign-Bousquet, M. (2011) Dynamic Analysis of the Lactococcus lactis Transcriptome in Cheeses Made from Milk Concentrated by Ultrafiltration Reveals Multiple Strategies of Adaptation to Stresses. Applied and Environmental Microbiology, 77, 247-257.

[48]   Suzzi, G. (2011) From Wild Strain to Domesticated Strain: The Philosophy of Microbial Diversity in Foods. Frontiers in Microbiology, 2, 169.

[49]   Giraffa, G. (2004) Studying the Dynamics of Microbial Populations during Food Fermentation. FEMS Microbiology Reviews, 28, 251-260.

[50]   Cogan, T.M., Beresford, T.P., Steele, J., Broadbent, J., Shah, N.P. and Ustunol, Z. (2007) Invited Review: Advances in Starter Cultures and Cultured Foods. Journal of Dairy Science, 90, 4005-4021.

[51]   Angelier, E. (2008) Les Sciences de la complexité et le vivant. Tec & Doc, Paris.

[52]   Vera, A. (2011) Etude de l’écosystème levain de panification. Incidence de l’échelle de fermentation sur la composition physico-chimique et microbiologique des levains. Ph.D. Dissertation, University Claude Bernard Lyon 1, Lyon.

[53]   de Vuyst, L., Vrancken, G., Ravyts, F., Rimaux, T. and Weckx, S. (2009) Biodiversity, Ecological Determinants, and Metabolic Exploitation of Sourdough Microbiota. Food Microbiology, 26, 666-675.

[54]   Meignen, B., Onno, B., Gelinas, P., Infantes, M., Guilois, S. and Cahagnier, B. (2001) Optimization of Sourdough Fermentation with Lactobacillus brevis and Baker’s Yeast. Food Microbiology, 18, 239-245.

[55]   Reinheimer, J.A., Quiberoni, A., Tailliez, P., Binetti, A.G. and Suárez, V.B. (1996) The Lactic Acid Microflora of Natural Whey Starters Used in Argentina for Hard Cheese Production. International Dairy Journal, 6, 869-879.

[56]   Smit, G., Smit, B.A. and Engels, W.J.M. (2005) Flavour Formation by Lactic Acid Bacteria and Biochemical Flavour Profiling of Cheese Products. FEMS Microbiology Reviews, 29, 591-610.

[57]   Montel, M.C., Bouton, Y. and Parguel, P. (2012) Ecosystèmes des laits et des fromages au lait cru—Enjeux pour leur ma?trise. In: Actes des 13ème Rencontres Recherche Ruminant, Paris.

[58]   Millet, L., Saubusse, M., Didienne, R., Tessier, L. and Montel, M.C. (2006) Control of Listeria monocytogenes in Raw-Milk Cheeses. International Journal of Food Microbiology, 108, 105-114.

[59]   Fayol-Messaoudi, D., Berger, C.N., Coconnier-Polter, M.H., Liévin-Le Moal, V. and Servin, A.L. (2005) pH-, Lactic Acid-, and Non-Lactic Acid-Dependent Activities of Probiotic Lactobacilli against Salmonella enterica Serovar Typhimurium. Applied and Environmental Microbiology, 71, 6008-6013.

[60]   Haines, W.C. and Harmon, L.G. (1973) Effect of Selected Lactic Acid Bacteria on Growth of Staphylococcus aureus and Production of Enterotoxin. Applied Microbiology, 25, 436-441.

[61]   Ly-Chatain, M.H., Durand, L., Rigobello, V., Vera, A. and Demarigny, Y. (2011) Direct Quantitative Detection and Identification of Lactococcal Bacteriophages from Milk and Whey by Real-Time PCR: Application for the Detection of Lactococcal Bacteriophages in Goat’s Raw Milk Whey in France. International Journal of Microbiology, 2011, Article ID: 594369.

[62]   Demarigny, Y., Poizat, M., Huguet, M., Le Coq, H. and Guegen, M. (2004) Evolution de la composition de la flore lactobacille thermophile cultivée sur lactosérum cru au cours de fabrications successives de fromages à pate pressée cuite dure. 12 ème Club des Bactéries Lactiques, Aurillac.

[63]   Capozzi, V. and Spano, G. (2011) Food Microbial Biodiversity and “Microbes of Protected Origin”. Frontiers in Microbiology, 2, 237.