Immunohistochemical Localization of Versican, Link Protein and Hyaluronic Acid Binding Protein in the Human Periodontal Ligament
Author(s)
Tadahiko Utsunomiya,
Aki Otsuka,
Rei Sato,
Masaru Yamaguchi,
Kazutaka Kasai,
Hirotsugu Yamamoto,
Kayo Kuyama
Affiliation(s)
Department of Oral Pathology, Nihon University School of Dentistry at Matsudo, Matsudo, Japan. Department of Orthodontics, Nihon University School of Dentistry at Matsudo, Matsudo, Japan. Nihon University, Tokyo, Japan.
Department of Oral Pathology, Nihon University School of Dentistry at Matsudo, Matsudo, Japan. Department of Orthodontics, Nihon University School of Dentistry at Matsudo, Matsudo, Japan. Nihon University, Tokyo, Japan.
ABSTRACT
The purpose of the present study was to histopathologically and immunohistochemically investigate the distribution of proteoglycans in human periodontal ligament (PDL). Specimens from osteotomy and tooth extraction having healthy PDL were studied. Histologically, PDL consisted of fibrous tissues, involving a compact arrangement area and edematous or myxoid area. Immuno-histochemically, versican binding region (12C5), versican link protein (8A4) and biotinylated hyaluronic acid binding protein (B-HABP) were distributed in PDL. In addition, positive immunore-activity for 12C5 and 8A4 was stronger in the compact arrangement area than in the edematous or myxoid area. Reactivity for B-HABP was stronger in the edematous or myxoid area than in the compact fibrous area. These results suggest that versican and link protein are associated with fibrous tissues, whereas hyaluronic acid is related to the formation of edematous and/or myxoid tissue in human PDL. These substances may play a role in periodontal homeostasis by protecting against mechanical stress.
The purpose of the present study was to histopathologically and immunohistochemically investigate the distribution of proteoglycans in human periodontal ligament (PDL). Specimens from osteotomy and tooth extraction having healthy PDL were studied. Histologically, PDL consisted of fibrous tissues, involving a compact arrangement area and edematous or myxoid area. Immuno-histochemically, versican binding region (12C5), versican link protein (8A4) and biotinylated hyaluronic acid binding protein (B-HABP) were distributed in PDL. In addition, positive immunore-activity for 12C5 and 8A4 was stronger in the compact arrangement area than in the edematous or myxoid area. Reactivity for B-HABP was stronger in the edematous or myxoid area than in the compact fibrous area. These results suggest that versican and link protein are associated with fibrous tissues, whereas hyaluronic acid is related to the formation of edematous and/or myxoid tissue in human PDL. These substances may play a role in periodontal homeostasis by protecting against mechanical stress.
Cite this paper
Utsunomiya, T. , Otsuka, A. , Sato, R. , Yamaguchi, M. , Kasai, K. , Yamamoto, H. and Kuyama, K. (2014) Immunohistochemical Localization of Versican, Link Protein and Hyaluronic Acid Binding Protein in the Human Periodontal Ligament. Open Journal of Stomatology, 4, 446-451. doi: 10.4236/ojst.2014.49060.
Utsunomiya, T. , Otsuka, A. , Sato, R. , Yamaguchi, M. , Kasai, K. , Yamamoto, H. and Kuyama, K. (2014) Immunohistochemical Localization of Versican, Link Protein and Hyaluronic Acid Binding Protein in the Human Periodontal Ligament. Open Journal of Stomatology, 4, 446-451. doi: 10.4236/ojst.2014.49060.
References
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http://dx.doi.org/10.1007/s004290050208 [5] Imai, K., Shibutani, T., Horiki, I., Ishihara, T., Kanazawa, A. and Iwayama, Y. (1998) Localization of Hyaluronan in Periodontal Tissues. Journal of Gifu Dental Society, 25, 28-34. [6] Fujii, T. and Hirabayashi, Y. (1999) Histochemical Studies of Glycosaminoglycans in Developing Periodontal Ligaments of ICR mice. The Anatomical Record, 254, 465-473. [7] Yamauchi, S., Cheng, H., Neame, P., Caterson, B. and Yamauchi, M. (1997) Identification, Partial Characterization, and Distribution of Versican and Link Protein in Bovine Dental Palp. Journal of Dental Research, 76, 1730-1736.
http://dx.doi.org/10.1177/00220345970760110301 [8] Shibata, S., Yoneda, S., Yanagishita, M. and Yamashita, Y. (2000) Isolation of Proteoglycan (Versican) Aggregates from Rat Dental Pulp. Archives of Oral Biology, 45, 563-568. [9] Hakkinen, L., Oksala, O., Salo, T., Rahemtulla, F. and Larjava, H. (1993) Immunohistochemical Localization of Proteoglycans in Human Periodontium. Journal of Histochemistry and Cytochemistry, 41, 1689-1699. [10] Shafer, W.G., Hine, M.K., Levy, B.M. and Tomich, C.E. (1983) Textbook of Oral Pathology. 4th Edition, W. B. Saunders Company, Philadelphia, 160-161. [11] Tomich, C.E. (1974) Oral Focal Mucinosis. A Clinicopathological and Histochemical Study of Eight Cases. Oral Surgery Oral Medicine Oral Pathology, 38, 714-724. [12] Ozawa, K. and Utsunomiya, T. (2000) A Histochemical and Immunohistochemical Study of Oral Neurofibroma and Schwannoma. With Special Reference to Mucoid Matrix Formation. Nihon University Journal of Oral Science, 26, 156-164. [13] Zhao, M., Lu, Y., Takata, T., Ogawa, I., Miyauchi, M., Mock, D. and Nikai, H. (1999) Immunohistochemical and Histochemical Characterization of the Mucosubstances of Odontogenic Myxoma. Histogenesis and Differential Diagnosis. Pathology, Research and Practice, 195, 391-397. [14] Alimohamad, H., Habijanac, T., Larjava, H. and Hakkinen, L. (2005) Colocalization of the Collagen-Binding Proteoglycans Decorin, Biglycan, Fibromodulin and Lumican with Different Cells in Human Gingiva. Journal of Periodontal Research, 40, 73-86. [15] Sato, R., Yamamoto, H., Kasai, K. and Yamauchi, M. (2002) Distribution Pattern of Versican, Link Protein and Hyaluronic Acid in the Rat Periodontal Ligament During Experimental Tooth Movement. Journal of Periodontal Research, 37, 15-22. [16] Nakamura, M., Sone, S., Takahashi, I., Mizoguchi, I., Echigo, S. and Sasano, Y. (2005) Expression of Versican and ADAMTS 1, 4, and 5 During Bone Development in the Rat Mandible and Hind Limb. Journal of Histochemistry & Cytochemistry, 53, 1553-1562. [17] Okahata, S., Yamamoto, R., Yamakoshi, Y. and Fukae, M. (2011) A Large Chondroitin Sulfate Proteoglycan, Versican, in Porcine Predentin. Journal of Oral Bioscience, 53, 72-81. [18] Beikler, T., Peters, U., Prior, K., Eisenacher, M. and Flemming T.F. (2008) Gene Expression in Priodontal Tissues Following Treatment. BMC Medical Genimics, 1, 30.
[1] Yanagishita, M. (1993) Function of Prteoglycans in the Extracellular Matrix. Acta Pathologica Japonica, 43, 283-293. [2] Heinegard, D. and Oldberg, A. (1993) Connective Tissue and Its Heritable Disorders. Wiley-Liss, New York, 283-209. [3] Reardon, A., Heinegard, D., Mcleod, D., Sheehan, J.K. and Bishop, P.N. (1998) The Large Chondroitin Sulphate Proteoglycan Versican in Mammalian Viterous. Matrix Biology, 17, 325-333. [4] Sorrell, J.M., Carrino, D.A., Baber, M.A. and Caplan, A.I. (1999) Versican in Human Fetal Skin Development. Anatomy and Embryology, 199, 45-56.
http://dx.doi.org/10.1007/s004290050208 [5] Imai, K., Shibutani, T., Horiki, I., Ishihara, T., Kanazawa, A. and Iwayama, Y. (1998) Localization of Hyaluronan in Periodontal Tissues. Journal of Gifu Dental Society, 25, 28-34. [6] Fujii, T. and Hirabayashi, Y. (1999) Histochemical Studies of Glycosaminoglycans in Developing Periodontal Ligaments of ICR mice. The Anatomical Record, 254, 465-473. [7] Yamauchi, S., Cheng, H., Neame, P., Caterson, B. and Yamauchi, M. (1997) Identification, Partial Characterization, and Distribution of Versican and Link Protein in Bovine Dental Palp. Journal of Dental Research, 76, 1730-1736.
http://dx.doi.org/10.1177/00220345970760110301 [8] Shibata, S., Yoneda, S., Yanagishita, M. and Yamashita, Y. (2000) Isolation of Proteoglycan (Versican) Aggregates from Rat Dental Pulp. Archives of Oral Biology, 45, 563-568. [9] Hakkinen, L., Oksala, O., Salo, T., Rahemtulla, F. and Larjava, H. (1993) Immunohistochemical Localization of Proteoglycans in Human Periodontium. Journal of Histochemistry and Cytochemistry, 41, 1689-1699. [10] Shafer, W.G., Hine, M.K., Levy, B.M. and Tomich, C.E. (1983) Textbook of Oral Pathology. 4th Edition, W. B. Saunders Company, Philadelphia, 160-161. [11] Tomich, C.E. (1974) Oral Focal Mucinosis. A Clinicopathological and Histochemical Study of Eight Cases. Oral Surgery Oral Medicine Oral Pathology, 38, 714-724. [12] Ozawa, K. and Utsunomiya, T. (2000) A Histochemical and Immunohistochemical Study of Oral Neurofibroma and Schwannoma. With Special Reference to Mucoid Matrix Formation. Nihon University Journal of Oral Science, 26, 156-164. [13] Zhao, M., Lu, Y., Takata, T., Ogawa, I., Miyauchi, M., Mock, D. and Nikai, H. (1999) Immunohistochemical and Histochemical Characterization of the Mucosubstances of Odontogenic Myxoma. Histogenesis and Differential Diagnosis. Pathology, Research and Practice, 195, 391-397. [14] Alimohamad, H., Habijanac, T., Larjava, H. and Hakkinen, L. (2005) Colocalization of the Collagen-Binding Proteoglycans Decorin, Biglycan, Fibromodulin and Lumican with Different Cells in Human Gingiva. Journal of Periodontal Research, 40, 73-86. [15] Sato, R., Yamamoto, H., Kasai, K. and Yamauchi, M. (2002) Distribution Pattern of Versican, Link Protein and Hyaluronic Acid in the Rat Periodontal Ligament During Experimental Tooth Movement. Journal of Periodontal Research, 37, 15-22. [16] Nakamura, M., Sone, S., Takahashi, I., Mizoguchi, I., Echigo, S. and Sasano, Y. (2005) Expression of Versican and ADAMTS 1, 4, and 5 During Bone Development in the Rat Mandible and Hind Limb. Journal of Histochemistry & Cytochemistry, 53, 1553-1562. [17] Okahata, S., Yamamoto, R., Yamakoshi, Y. and Fukae, M. (2011) A Large Chondroitin Sulfate Proteoglycan, Versican, in Porcine Predentin. Journal of Oral Bioscience, 53, 72-81. [18] Beikler, T., Peters, U., Prior, K., Eisenacher, M. and Flemming T.F. (2008) Gene Expression in Priodontal Tissues Following Treatment. BMC Medical Genimics, 1, 30.