FNS  Vol.5 No.17 , September 2014
Comparison of Total Phenolic Content and Total Antioxidant Activity in Local Red Wines Determined by Spectrophotometric Methods
Abstract: In this study, the antioxidant activity of local red wines was determined by four different analytical methods: 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging assay, 2,2'-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS?+) radical cation decolourisation, ferric reducing/antioxidants power (FRAP) and cupric ion reducing capacity in the presence of neocuproine (CUPRAC) for the evaluation of reducing power (PR). They were correlated with the total phenolic content (TPC) determined with the Folin-Ciocalteu’s reagent using gallic acid as a standard. The total polyphenol concentration was found to vary from 2599.90 to 4846.57 mg/L gallic acid equivalents (GAE). The total antioxidant activity determined by DPPH, ABTS, FRAP and CUPRAC methods was found to vary from 7.49 - 15.93 mmol/L, 12.02 - 24.73 mmol/L, 12.65 - 27.68 mmol/L and 13.19 - 31.07 mmol/L, respectively. The total phenolic contents of red wine samples exhibited a good correlation (p < 0.01) with antioxidant properties.
Cite this paper: Büyüktuncel, E. , Porgalı, E. and Çolak, C. (2014) Comparison of Total Phenolic Content and Total Antioxidant Activity in Local Red Wines Determined by Spectrophotometric Methods. Food and Nutrition Sciences, 5, 1660-1667. doi: 10.4236/fns.2014.517179.

[1]   Becker, E.M., Nissen, L.R. and Skibsted, L.H. (2004) Antioxidant Evaluation Protocols: Food Quality or Health Effects. European Food Research and Technology, 219, 561-571.

[2]   Ratnam, D.V., Ankola, D.D., Bhardwaj, V., Sahana, D.K. and Kumar, M.N.V.R. (2006) Role of Antioxidants in Prophylaxis and Therapy: A Pharmaceutical Perspective. Journal of Controlled Release, 113, 189-207.

[3]   Lopez-Velez, M., Martinez-Martinez, F. and Del Valle-Ribes, C. (2003) The Study of Phenolic Compounds as Natural Antioxidants in Wine. Critical Reviews in Food Science and Nutrition, 43, 233-244.

[4]   Steffen, L.M., Jacobs, D.R., Stevens, J., Shahar, E., Carithers, T. and Folsom, A.R. (2003) Associations of Whole- Grain, Refined-Grain, and Fruit and Vegetable Consumption with Risks of All-Cause Mortality and Incident Coronary Artery Disease and Îschemic Stroke: The Atherosclerosis Risk in Communities (ARIC) Study. The American Journal of Clinical Nutrition, 78, 383-390.

[5]   Paixao, N., Perestrelo, R., Marques, J.C. and Camara, J.S. (2007) Relationship between Antioxidant Capacity and Total Phenolic Content of Red, Rose and White Wines. Food Chemistry, 105, 204-214.

[6]   Seruga, M., Novak, I. and Jakobek, L. (2011) Determination of Polyphenols Content and Antioxidant Activity of Some Red Wines by Differential Pulse Voltammetry, HPLC and Spectrophotometric Methods. Food Chemistry, 124, 1208- 1216.

[7]   Woraratphoka, J., Intarapichet, K.O. and Indrapichate, K. (2007) Phenolic Compounds and Antioxidative Properties of Selected Wines from the Northeast of Thailand. Food Chemistry, 104, 1485-1490.

[8]   Stasko, A., Brezova, V., Mazur, M., Certik, M., Kalinak, M. and Gescheidt, G. (2008) A Comparative Study on the Antioxidant Properties of Slovakian and Austrian Wines. LWT—Food Science and Technology, 41, 2126-2135.

[9]   Li, H., Wang, X.Y., Li, Y., Li, P.H. and Wang, H. (2009) Polyphenolic Compounds and Antioxidant Properties of Selected China Wines. Food Chemistry, 112, 454-460.

[10]   Lucena, A.P.S., Nascimento, R.J.B., Maciel, J.A.C., Tavares, J.X., Barbosa, J.M. and Oliveira, E.J. (2010) Antioxidant Activity and Phenolics Content of Selected Brazilian Wines. Journal of Food Composition and Analysis, 23, 30-36.

[11]   Vrcek, I.V., Bojic, M., Zuntar, I., Mendas, G. and Medic-Saric, M. (2011) Phenol Content, Antioxidant Activity and Metal Composition of Croatian Wines Deriving from Organically and Conventionally Grown Grapes. Food Chemistry, 124, 354-361.

[12]   Stratil, P., Kuban, V. and Fojtova, J. (2008) Comparison of the Phenolic Content and Total Antioxidant Activity in Wines as Determined by Spectrophotometric Methods. Czech Journal of Food Sciences, 26, 242-253.

[13]   Bondet, V., Brand-Williams, W. and Berset, C. (1997) Kinetics and Mechanisms of Antioxidant Activity Using the DPPH Free Radical Method. LWT-Food Science and Technology, 30, 609-615.

[14]   Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M. and Rice-Evans, C. (1999) Antioxidant Activity Applying an Improved ABTS Radical Cation Decolorization Assay. Free Radical Biology and Medicine, 26, 1231-1237.

[15]   Benzie, I.F.F. and Strain, J.J. (1996) The Ferric Reducing Ability of Plasma (FRAP) as a Measure of “Antioxidant Power”: The FRAP Assay. Analytical Biochemistry, 239, 70-76.

[16]   Pulido, R., Bravo, L. and Saura-Calixto, F. (2000) Antioxidant Activity of Dietary Polyphenols as Determined by a Modified Ferric Reducing/Antioxidant Power Assay. Journal of Agricultural and Food Chemistry, 48, 3396-3402.

[17]   Apak, R., Guclu, K., Ozyurek, M. and Karademir, S.E. (2004) Novel Total Antioxidant Capacity Îndex for Dietary Polyphenols and Vitamins C and E, Using Their CupricÎon Reducing Capability in the Presence of Neocuproine: CUPRAC Method. Journal of Agricultural and Food Chemistry, 52, 7970-7981.

[18]   Cao, G.H. and Prior, R.L. (1999) Measurement of Oxygen Radical Absorbance Capacity in Biological Samples. Methods in Enzymology, 299, 50-62.

[19]   Naguib, Y.M.A. (2000) A Fluorometric Method for Measurement of Oxygen Radical-Scavenging Activity of WaterSoluble Antioxidants. Analytical Biochemistry, 284, 93-98.

[20]   Pérez-Jiméneza, J., Arranz, S., Tabernero, M., Diaz-Rubio, M.E., Serrano, J., Goni, I. and Saura-Calixto, F. (2008) Updated Methodology to Determine Antioxidant Capacity in Plant Foods, Oils and Beverages: Extraction, Measurement and Expression of. Food Research International, 41, 274-285.

[21]   Matthaus, B. (2002) Antioxidant Activity of Extracts Obtained from Residues of Different Oilseeds. Journal of Agricultural and Food Chemistry, 50, 3444-3452.

[22]   Nixdorf, S.L. and Hermosin-Gutierrez, I. (2010) Brazilian Red Wines Made from the Hybrid Grape Cultivar Isabel: Phenolic Composition and Antioxidant Capacity. Analytica Chimica Acta, 659, 208-215.

[23]   Fernández-Pachón, M.S., Villano, D., GarcÍa-Parrilla, M.C. and Troncoso, A.M. (2004) Antioxidant Activity of Wines and Relation with Their Polyphenolic Composition. Analytica Chimica Acta, 513, 113-118.

[24]   Kallithraka, S., Tsoutsouras, E., Tzourou, E. and Lanaridis, P. (2006) Principal Phenolic Compounds in Greek Red Wines. Food Chemistry, 99, 784-793.

[25]   Di Majo, D., La Guardia, M., Giammanco, S., La Neve, L., Giammanco, M. (2008) The Antioxidant Capacity of Red Wine in Relationship with Its Polyphenolic Constituents. Food Chemistry, 111, 45-49.

[26]   Minussi, R.C., Rossi, M., Bologna, L., Cordi, L., Rotilio, D., Pastore, G.M. and Duran, N. (2003) Phenolic Compounds and Total Antioxidant Potential of Commercial Wines. Food Chemistry, 82, 409-416.

[27]   Dugo, G., Dugo, P., Vilasi, F., Magnisi, R., Mondello, L. and La Torre, G.L. (2006) Determination of the Polyphenolic Content in Sicilian Red Wines of Protected Geographical Indication. Italian Journal of Food Science, 18, 409-422.

[28]   Jordao, A.M., Goncalves, F.J., Correia, A.C., Cantao, J., Rivero-Perez, M.D. and SanJose, M.L.G. (2010) Proanthocyanidin Content, Antioxidant Capacity and Scavenger Activity of Portuguese Sparkling Wines (Bairrada Appellation of Origin). Journal of the Science of Food and Agriculture, 90, 2144-2152.

[29]   Yoo, Y.J., Prenzler, P.D., Saliba, A.J. and Ryan, D. (2011) Assessment of Some Australian Red Wines for Price, Phenolic Content, Antioxidant Activity, and Vintage in Relation to Functional Food Prospects. Journal of Food Science, 76, C1355-C1364.

[30]   Jiang, B. and Zhang, Z.W. (2012) Comparison on Phenolic Compounds and Antioxidant Properties of Cabernet Sauvignon and Merlot Wines from Four Wine Grape-Growing Regions in China. Molecules, 17, 8804-8821.

[31]   Radovanovic, A.N., Jovancicevic, B.S., Radovanovic, B.C., Mihajilov-Krstev, T. and Zvezdanovic, J.B. (2012) Antioxidant and Antimicrobial Potentials of Serbian Red Wines Produced from International Vitis Vinifera Grape Varieties. Journal of the Science of Food and Agriculture, 92, 2154-2161.

[32]   Porgali, E. and Buyuktuncel, E. (2012) Determination of Phenolic Composition and Antioxidant Capacity of Native Red Wines by High Performance Liquid Chromatography and Spectrophotometric Methods. Food Research International, 45, 145-154.

[33]   De Beer, D., Joubert, E., Gelderblom, W.C.A. and Manley, M. (2003) Antioxidant Activity of South African Red and White Cultivar Wines: Free Radical Scavenging. Journal of Agricultural and Food Chemistry, 51, 902-909.

[34]   Feliciano, R.P., Bravo, M.N., Pires, M.M., Serra, A.T., Duarte, C.M., Boas, L.V. and Bronze, M.R. (2009) Phenolic Content and Antioxidant Activity of Moscatel Dessert Wines from the Setubal Region in Portugal. Food Analyt?cal Methods, 2, 149-161.

[35]   Xi, Z.M., Meng, J.F., Huo, S.S., Luan, L.Y., Ma, L.N. and Zhang, Z.W. (2013) Exogenously Applied Abscisic Acid to Yan73 (V. vinifera) Grapes Enhances Phenolic Content and Antioxidant Capacity of Its Wine. International Journal of Food Sciences Nutrition, 64, 444-451.