Analysis of Diagnostic Efficiency of Excretory-Secretory Antigens for Trichinella spiralis and Trichinella nativa in ELISA

Irina  Odoevskaya; Sergey  Movsesyan; Mikhail  Voronin; Nataya  Chiljuta

doi:10.4236/ojvm.2014.49021

Irina Odoevskaya, Sergey Movsesyan, Mikhail Voronin, Nataya Chiljuta

Abstract: The comparative studies of diagnostic efficiency of excretory-secretory antigens of Trichinella spiralis and Trichinella nativa were performed using blood sera of rats from Wistar line experimentally infected with Arctic trichinellae. For animal infection and antigen preparation Trichinella from muscles of wild carnivorous mammals from Arctic regions of Russia were used. When antigen from T. nativa larvae was used to analyze titers of sera of rats experimentally infected with Arctic Trichinella, a significant increase in efficacy ofELISAwas detected. E.g., sera of rats infected with trichinellae from ringed seals retained in ELISA with T. nativa antigen values higher than diagnostic level at titers of 1:6400 - 1:12800, while titer of those same sera when using T. spiralis antigen was no higher than 1:200 - 1:400.

Keywords: ELISA, Trichinella nativa, Diagnostic Efficiency, Laboratory Infection, Wistar Rats, Blood Sera, Excretory-Secretory Antigen

Cite this paper: Odoevskaya, I. , Movsesyan, S. , Voronin, M. and Chiljuta, N. (2014) Analysis of Diagnostic Efficiency of Excretory-Secretory Antigens for Trichinella spiralis and Trichinella nativa in ELISA. Open Journal of Veterinary Medicine, 4, 183-189. doi: 10.4236/ojvm.2014.49021.

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