ABB  Vol.5 No.9 , August 2014
Evaluation of IL-6 and IL-8 in Tear Fluid of Sulfur Mustard Gas-Exposed Patients with Eye Lesions
ABSTRACT

The purpose of this study is to compare the levels of IL-6, 8 in tear fluids of people exposed to mustard gas in the war between Iraq and Iran who had the chronic dry-eye symptoms compared to the normal group. In this study, 25 of the patients who were exposed to mustard gas and had chronic dry eye symptoms were compared to 25 patients as control group, consisting of 25 people who had common chronic dry eye symptoms with blepharitis and 25 healthy people as normal group. The levels of IL-6 and IL-8 in tear fluid of people of these three groups were assessed by enzyme-linked immunosorbent assay (ELISA). The results for levels of IL-6 (P = 0.002) and IL-8 (P = 0.001) in tear fluid of patients in comparison with normal group show a significant increase. The differences were considered statistically significant at P < 0.05. The effect of exposure to mustard gas on eyes of chemical-injured veterans destroyed meibomian glands which paved the way for evaporative type of dry-eye. As a result, the cited ILs in the tear fluid of these patients increased and resulted in later eye-impairments.


Cite this paper
Hajihosseini, R. , Shohrati, M. , Naderi, M. , Azimi, N. , Esfandiari, M. and Najafian, B. (2014) Evaluation of IL-6 and IL-8 in Tear Fluid of Sulfur Mustard Gas-Exposed Patients with Eye Lesions. Advances in Bioscience and Biotechnology, 5, 790-795. doi: 10.4236/abb.2014.59092.
References
[1]   Mahmoudi, M., Hefazib, M., Rastina, M. and Balali-Mood, M. (2005) Long-Term Hematological Unological Complications of Sulfur Mustard Poisoning in Iranian Veterans. International Immunopharmacology, 5, 1479-1485.
http://dx.doi.org/10.1016/j.intimp.2005.04.003

[2]   Gosden, C.M. (2001) The 1988 Chemical Weapons Attack on Halabja, Iraq. In: Alexander, Y. and Hoening, M., Eds., Super Terrorism: Biological Weapons, Chemical and Nuclear, Transnational Publishers, Ardsley, 7-12.

[3]   Dacre, J.C. and Goldman, M. (1996) Toxicology and Pharmacology of the Chemical Warfare Agent Sulfur Mustard. Pharmacological Reviews, 48, 289-326.

[4]   Shohrati, M., Ghanei, M., Shamspour, N., Babaei, F., badi, M.N., et al. (2010) Glutathione and Malondialdehyde Levels in Late Pulmonary Complications of Sulfur Mustard Intoxication. Lung, 188, 77-83.

[5]   Shohrati, M., Ghanei, M., Harandi, A.A., Foroghi, S. and Harandi, A.A. (2012) Effect of Nebulized Morphine on Dyspnea of Mustard Gas-Exposed Patients: A Double-Blind Randomized Clinical Trial Study. Pulmonary Medicine, 2012, Article ID: 610921, 6 p.

[6]   Shohrati, M., Ghanei, M., Shamspour, N. and Jafari, M. (2008) Activity and Function in Lung Injuries Due to Sulphur Mustard. Biomarkers, 13, 728-733.

[7]   Maynard, R.L., Meredith, T.J., Marrs, T.C. and Vale, J.A. (1991) Management of War Injuries. The Lancet, 337, 122.

[8]   Balali-Mood, M. and Hefazi, M. (2006) Comparison of Early and Late Toxic Effects of Sulfur Mustard in Iranian Veterans. Basic & Clinical Pharmacology & Toxicology, 99, 273-282.
http://dx.doi.org/10.1111/j.1742-7843.2006.pto_429.x

[9]   Khaheshi, I., Keshavarz, S., Ali Imani Fooladi, A., Ebrahimi, M., Yazdani, S., et al. (2011) Loss of Expression of TGF-bs and Their Receptors in Chronic Skin Lesions Induced by Sulfur Mustard as Compared with Chronic Contact Dermatitis Patients. BMC Dermatology, 11, 2.

[10]   Geeraets, W.J., Abedi, S. and Blanke, R.V. (1977) Acute Corneal Injury by Mustard Gas. Southern Medical Journal, 70, 348-351.
http://dx.doi.org/10.1097/00007611-197703000-00028

[11]   Dahl, H., Gluud, B., Vangested, P. and Norn, M. (1985) Eye Lesions Induced by Mustard Gas. Acta Ophthalmologica, 173, S30-S31.

[12]   Mann, I., Pirie, A. and Pullinger, B.D. (1948) An Experimental and Clinical Study of the Reaction of the Anterior Segment of the Eye to Chemical Injury, with Special References to Chemical Warfare Agents. British Journal of Ophthalmology, Monograph Supplement XIII.

[13]   Massingale, M.L., Li, X., Vallabhajosyula, M., Chen, D., Wei, Y. and Asbell, P.A. (2009) Analysis of Inflammatory Cytokines in the Tears of Dry Eye Patients. Cornea, 28, 1023-1027.

[14]   Stern, M.E., Gao, J., Schwalb, T.A., Ngo, M., Tieu, D.D., Chan, C.C., Reis, B.L., Whitcup, S.M., Thompson, D. and Smith, J.A. (2002) Conjunctival T-Cell Subpopulations in Sjogren’s and Non-Sjogren’s Patients with Dry Eye. Investigative Ophthalmology & Visual Science, 43, 2609-2614.

[15]   Rolando, M., Barabino, S., Mingari, C., Moretti, S., Giuffrida, S. and Calabria, G. (2005) Distribution of Conjunctival HLA-DR Expression and the Pathogenesis of Damage in Early Dry Eye. Cornea, 24, 951-954.
http://dx.doi.org/10.1097/01.ico.0000157421.93522.00

[16]   Ghasemi, H., Ghazanfari, T., Ghassemi-Broumand, M., Javadi, M.A., Babaei, M., Soroush, M.R., et al. (2009) Long-Term Ocular Consequences of Sulfur Mustard in Seriously Eye Injured War Veterans. Cutaneous and Ocular Toxicology, 28, 71-77.
http://dx.doi.org/10.1080/15569520902913936

[17]   Hosseini, R.H. and Esfandiary, M.A. (2012) Levels of Matrix Metalloproteinases (MMPs) in Tear Fluid of Mustard Gas Exposed Patients with Chronic Dry-Eye Symptoms. African Journal of Biochemistry Research, 6, 55-61.

[18]   McCully, J.P. and Shine, W. (1997) A Compositional Based Model for the Tear Film Lipid Layer. Transactions of the American Ophthalmological Society, 95, 79-88.

[19]   McCully, J.P. and Shine, W. (2002) Meibomian Gland and Tear Film Lipids: Structure, Function and Control, Lacrimal Gland, Tear Film, and Dry Eye Syndromes. Advances in Experimental Medicine and Biology, 506, 373-378.

[20]   Acera, A., Rocha, G., Vecino, E., Lema, I. and Durán, J.A. (2008) Inflammatory Markers in the Tears of Patients with Ocular Surface Disease. Ophthalmic Research, 40, 315-321.
http://dx.doi.org/10.1159/000150445

[21]   Afonso, A.A., Sobrin, L., Monroy, D.C., Selzer, M., Lokeshwar, B. and Pflugfelder, S.C. (1999) Tear Fluid Gelatinase B Activity Correlates with IL-1α Concentration and Fluorescein Clearance in Ocular Rosacea. Investigative Ophthalmology & Visual Science, 40, 2506-2512.

[22]   Solomon, A., Dursun, D., Liu, Z., Xie, Y., Macri, A. and Pflugfelder, S.C. (2001) Proand Anti-Inflammatory Forms of Interleukin-1 in the Tear Fluid and Conjunctiva of Patients with Dry-Eye Disease. Investigative Ophthalmology & Visual Science, 42, 2283-2292.

[23]   Luo, L., Li, D.Q., Doshi, A., Farley, W., Corrales, R.M. and Pflugfelder, S.C. (2004) Experimental Dry Eye Stimulates Production of Inflammatory Cytokines and MMP-9 and Activates MAPK Signaling Pathways on the Ocular Surface. Investigative Ophthalmology & Visual Science, 45, 4293-4301.
http://dx.doi.org/10.1167/iovs.03-1145

[24]   Trousdale, M.D., Zhu, Z., Stevenson, D., Schechter, J.E., Ritter, T. and Mircheff, A.K. (2005) Expression of TNF Inhibitor Gene in the Lacrimal Gland Promotes Recovery of Tear Production and Tear Stability and Reduced Immunopathology in Rabbits with Induced Autoimmune Dacryoadenitis. Journal of Autoimmune Diseases, 2, 6.
http://dx.doi.org/10.1186/1740-2557-2-6

[25]   Zhu, Z., Stevenson, D., Schechter, J.E., Mircheff, A.K., Ritter, T., Labree, L. and Trousdale, M.D. (2004) Prophylactic Effect of IL-10 Gene Transfer on Induced Autoimmune Dacryoadenitis. Investigative Ophthalmology & Visual Science, 45, 1375-1381.
http://dx.doi.org/10.1167/iovs.03-0755

[26]   Zoukhri, D., Hodges, R.R., Byon, D. and Kublin, C.L. (2002) Role of Proinflammatory Cytokines in the Impaired Lacrimation Associated with Autoimmune Xerophthalmia. Investigative Ophthalmology & Visual Science, 43, 1429-1436.

[27]   Lee, E.B., Kim, J.Y., Zhao, J., Park, M.H. and Song, Y.W. (2007) Haplotype Association of IL-8 Gene with Behcet’s Disease. Tissue Antigens, 69, 128-132.
http://dx.doi.org/10.1111/j.1399-0039.2006.00736.x

[28]   Belguendouz, H., Messaoudene, D., Hartani, D., Chachoua, L., Ahmedi, M.L., Lahmar-Belguendouz, K., Lahlou-Boukoffa, O. and Touil-Boukoffa, C. (2008) Effect of Corticotherapy on Interleukin-8 and -12 and Nitric Oxide Production during Behcxet and Idiopathic Uveitis. Journal Français d’Ophtalmologie, 31, 387-395.
http://dx.doi.org/10.1016/S0181-5512(08)71433-5

 
 
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