Comparative Study of Bunt Pathogen Resistance to the Effects of Fungicides in Callus Co-Cultures Triticum aestivum with Tilletia caries
Author(s)
L. G. Yarullina,
R. I. Kasimova*,
B. R. Kuluev,
O. B. Surina,
L. M. Yarullina,
R. I. Ibragimov
Affiliation(s)
Institute of Biochemistry and Genetics of Ufa Scientific Center of Russian Academy of Sciences, Ufa, Russia. Bashkir State University, Ufa, Russia.
Institute of Biochemistry and Genetics of Ufa Scientific Center of Russian Academy of Sciences, Ufa, Russia. Bashkir State University, Ufa, Russia.
ABSTRACT
The morphophysiological and molecular-genetic parameters of T. caries isolates collected from various fields of Southern Urals agrocenoses have been analysed. Isolate 1 of wheat callus had a high growth rate in vitro even in the presence of 0.1 mg/l fungicide Baitan. Isolate 2 of wheat callus had a low growth rate whereas 0.1 mg/l Baitan significantly inhibited its growth. Comparison of nucleotide sequences of 18S RNA gene of the two isolates showed high level of homology between them, but a large number of nucleotide substitutions have been found. The most characteristic excision was five nucleotides at position 461 of the isolate 2, compared with the isolate 1. Our results allow to assume that the environmental stress including high pesticide concentration may cause the resistance of T. caries pathogen to fungicides.
Cite this paper
Yarullina, L. , Kasimova, R. , Kuluev, B. , Surina, O. , Yarullina, L. and Ibragimov, R. (2014) Comparative Study of Bunt Pathogen Resistance to the Effects of Fungicides in Callus Co-Cultures Triticum aestivum with Tilletia caries. Agricultural Sciences, 5, 906-912. doi: 10.4236/as.2014.510098.
Yarullina, L. , Kasimova, R. , Kuluev, B. , Surina, O. , Yarullina, L. and Ibragimov, R. (2014) Comparative Study of Bunt Pathogen Resistance to the Effects of Fungicides in Callus Co-Cultures Triticum aestivum with Tilletia caries. Agricultural Sciences, 5, 906-912. doi: 10.4236/as.2014.510098.
References
[1] Gici, U., Chet, I. and Gullino, M.L. (2009) Recent Development in Management of Plant Diseases. Springer, 392 p. [2] Karatigin, I.V. (1981) Smut Fungi (Ontogenesis and Phylogenesis). Nauka, Leningrad, 216 p. [3] Keon, J.P.R., White, G.A. and Hargreaves, J.A. (1991) Isolation, Characterization and Sequence of a Gene Conferring Resistance to the Systemic Fungicide Carboxin from the Maize Smut Pathogen Ustilago maydis. Current Genetics, 19, 475-481.
http://dx.doi.org/10.1007/BF00312739 [4] Orth, A.B., Rzhetskaya, M., Pell, E.J. and Tien, M. (1995) A Serine Protein Kinase Confers Fungicide Resistance in the Phytopathogenic Fungus Ustilago maydis. Appl. Environ. Microbiol, 61, 2341-2345. [5] Tuna, A.L. (2012) Comparative Activities of Triadimefon, Thidiazuron, and Chlorocholine Chloride as Salinity Stress Protectants in Maize (Zea mays L.) plant. Fresenius Environmental Bulletin, 21, 1598-1608. [6] Fletcher, R.A. and Hofstra, G. (1987) Triasoles as Plant Stress Protectants. Highlights of Agricultural Research, 10, 12-14. [7] Mysyakina, I.S. and Funtikova, N.S. (2007) The Role of Sterols in Morphogenetic Processes and Dimorphism in Fungi. Mikrobiologiya, 76, 1-13. [8] Garcia, P.C., Rivero, R.V., Ruiz, J.M. and Romero, L. (2001) The Role of Fungicides in the Physiology of Higher Plants: Implications for Defense Responses. The Botanical Review, 69, 162-172.
http://dx.doi.org/10.1663/0006-8101(2003)069[0162:TROFIT]2.0.CO;2 [9] Markoglou, A.N. and Ziogas, B.N. (2002) Genetic Control of Resistance to the Piperidine Fungicide Piperalin in Ustilago maydis. European Journal of Plant Pathology, 108, 21-30.
http://dx.doi.org/10.1023/A:1013972914944 [10] Weber, I., Abmann, D., Thines, E. and Steinberg, G. (2006) Polar Localizing Class V Myosin Synthases Are Essential during Early Plant Infection in the Plant Pathogenic Fungus Ustilago maydis. Plant Cell, 18, 225-242.
http://dx.doi.org/10.1105/tpc.105.037341 [11] Annamalai, P. and Lalithakumari, D. (1992) Development of Resistance of Bipolaris oryzae against Edifenphos. Mycological Research, 92, 454-460.
http://dx.doi.org/10.1016/S0953-7562(09)81090-3 [12] Troshina, N.B., Isaev, R.F., Yakhin, I.A. and Yakhin, O.I. (1998) Influence of Environmental Factors on the Growth and Development of Resistant to Baitan Strain of Fusarium graminearum and Septoria nodorum. Mikologia i Fitopatologia, 32, 58-62. [13] Palani, P.V. and Lalithakumari, D. (1999) Resistance of Venturia inaequalis to the Sterol Biosynthesis—Inhibiting Fungicide, Penconazole [1-(2-(2,4-dichlorophenyl)pentyl)-1H-1,2,4-triazole]. Mycological Research, 103, 1157-1164. [14] Troshina, N.B., Glukhen’kova, M.V., Maksimov, I.V., Surina, O.B. and Khairullin, R.M. (2000) Morphological Analysis of the Growth and Development of Tilletia caries (DC.) Tul. on Wheat Calluses. Mikologia i Fitopatologia, 34, 48-50. [15] Troshina, N.B., Yarullina, L.G., Surina, O.B. and Maksimov, I.V. (2006) Inducers of Plant Resistance and Reactive Oxygen Species. III. Influence Bisol 2 and Baitan on Morphogenesis and Cell Protective Response of Wheat Calluses Infected Smut Pathogen. Citologia, 48, 495-499. [16] Martinez-Espinoza, A.D., Garcia-Pedrajas, M.D. and Gold, S.E. (2002) The Ustilaginales as Plant Pests and Model Systems. Fungal Genetics and Biology, 35, 1-20.
http://dx.doi.org/10.1006/fgbi.2001.1301 [17] Krivchenko, V.I. (1984) Resistance to Pathogens of Cereal Smut Diseases. Kolos, Moscow, 304 p. [18] Borgen, A. (2004) Organic Seed Treatment to Control Common Bunt (Tilletia tritici) in Wheat. Seed Testing International, 128, 8-9. [19] Rupukhova, N.V. (2005) Environmental Protection Aspects of Winter Wheat Dwarf Bunt on Leached Chernozem of the Stavropol Territory. Candidate of Biology Science Thesis, Stavropol. [20] Gargas, A. and Taylor, J.W. (1992) Polymerase Chain Reaction (PCR) Primers for Amplifying and Sequencing 18S rDNA from Lichenized Fungi. Mycologia, 84, 589-592.
http://dx.doi.org/10.2307/3760327
[1] Gici, U., Chet, I. and Gullino, M.L. (2009) Recent Development in Management of Plant Diseases. Springer, 392 p. [2] Karatigin, I.V. (1981) Smut Fungi (Ontogenesis and Phylogenesis). Nauka, Leningrad, 216 p. [3] Keon, J.P.R., White, G.A. and Hargreaves, J.A. (1991) Isolation, Characterization and Sequence of a Gene Conferring Resistance to the Systemic Fungicide Carboxin from the Maize Smut Pathogen Ustilago maydis. Current Genetics, 19, 475-481.
http://dx.doi.org/10.1007/BF00312739 [4] Orth, A.B., Rzhetskaya, M., Pell, E.J. and Tien, M. (1995) A Serine Protein Kinase Confers Fungicide Resistance in the Phytopathogenic Fungus Ustilago maydis. Appl. Environ. Microbiol, 61, 2341-2345. [5] Tuna, A.L. (2012) Comparative Activities of Triadimefon, Thidiazuron, and Chlorocholine Chloride as Salinity Stress Protectants in Maize (Zea mays L.) plant. Fresenius Environmental Bulletin, 21, 1598-1608. [6] Fletcher, R.A. and Hofstra, G. (1987) Triasoles as Plant Stress Protectants. Highlights of Agricultural Research, 10, 12-14. [7] Mysyakina, I.S. and Funtikova, N.S. (2007) The Role of Sterols in Morphogenetic Processes and Dimorphism in Fungi. Mikrobiologiya, 76, 1-13. [8] Garcia, P.C., Rivero, R.V., Ruiz, J.M. and Romero, L. (2001) The Role of Fungicides in the Physiology of Higher Plants: Implications for Defense Responses. The Botanical Review, 69, 162-172.
http://dx.doi.org/10.1663/0006-8101(2003)069[0162:TROFIT]2.0.CO;2 [9] Markoglou, A.N. and Ziogas, B.N. (2002) Genetic Control of Resistance to the Piperidine Fungicide Piperalin in Ustilago maydis. European Journal of Plant Pathology, 108, 21-30.
http://dx.doi.org/10.1023/A:1013972914944 [10] Weber, I., Abmann, D., Thines, E. and Steinberg, G. (2006) Polar Localizing Class V Myosin Synthases Are Essential during Early Plant Infection in the Plant Pathogenic Fungus Ustilago maydis. Plant Cell, 18, 225-242.
http://dx.doi.org/10.1105/tpc.105.037341 [11] Annamalai, P. and Lalithakumari, D. (1992) Development of Resistance of Bipolaris oryzae against Edifenphos. Mycological Research, 92, 454-460.
http://dx.doi.org/10.1016/S0953-7562(09)81090-3 [12] Troshina, N.B., Isaev, R.F., Yakhin, I.A. and Yakhin, O.I. (1998) Influence of Environmental Factors on the Growth and Development of Resistant to Baitan Strain of Fusarium graminearum and Septoria nodorum. Mikologia i Fitopatologia, 32, 58-62. [13] Palani, P.V. and Lalithakumari, D. (1999) Resistance of Venturia inaequalis to the Sterol Biosynthesis—Inhibiting Fungicide, Penconazole [1-(2-(2,4-dichlorophenyl)pentyl)-1H-1,2,4-triazole]. Mycological Research, 103, 1157-1164. [14] Troshina, N.B., Glukhen’kova, M.V., Maksimov, I.V., Surina, O.B. and Khairullin, R.M. (2000) Morphological Analysis of the Growth and Development of Tilletia caries (DC.) Tul. on Wheat Calluses. Mikologia i Fitopatologia, 34, 48-50. [15] Troshina, N.B., Yarullina, L.G., Surina, O.B. and Maksimov, I.V. (2006) Inducers of Plant Resistance and Reactive Oxygen Species. III. Influence Bisol 2 and Baitan on Morphogenesis and Cell Protective Response of Wheat Calluses Infected Smut Pathogen. Citologia, 48, 495-499. [16] Martinez-Espinoza, A.D., Garcia-Pedrajas, M.D. and Gold, S.E. (2002) The Ustilaginales as Plant Pests and Model Systems. Fungal Genetics and Biology, 35, 1-20.
http://dx.doi.org/10.1006/fgbi.2001.1301 [17] Krivchenko, V.I. (1984) Resistance to Pathogens of Cereal Smut Diseases. Kolos, Moscow, 304 p. [18] Borgen, A. (2004) Organic Seed Treatment to Control Common Bunt (Tilletia tritici) in Wheat. Seed Testing International, 128, 8-9. [19] Rupukhova, N.V. (2005) Environmental Protection Aspects of Winter Wheat Dwarf Bunt on Leached Chernozem of the Stavropol Territory. Candidate of Biology Science Thesis, Stavropol. [20] Gargas, A. and Taylor, J.W. (1992) Polymerase Chain Reaction (PCR) Primers for Amplifying and Sequencing 18S rDNA from Lichenized Fungi. Mycologia, 84, 589-592.
http://dx.doi.org/10.2307/3760327