NS  Vol.6 No.12 , August 2014
Enhancement of Cell Growth and Lipid Content of a Freshwater Microalga Scenedesmus sp. by Optimizing Nitrogen, Phosphorus and Vitamin Concentrations for Biodiesel Production
Abstract: The effects of nitrogen, phosphorus and vitamin on growth and lipid content of a freshwater microalgae Scenedesmus sp. were investigated under indoor conditions. Nitrogen, phosphorus and vitamin presented significant statistical effect on lipid content, and a maximum lipid content of 29.3% was obtained, corresponded to increases of 90% when compared to those obtained with the original nutrients medium. On the other hand, only nitrogen and phosphorus presented significant statistical effect on cell growth, achieving ash free dry biomass, algal density, and specific growth rate of 1.3 g·L-1, 1.5E+07 cells mL-1, and 0.62 d-1, respectively. By the use of the desirability function, it was possible to maximize the cell growth and lipid content simultaneously. The fatty acid composition of the microalgal lipid comprises over 80% of saturated and monounsaturated fatty acids. Thus, Scenedesmus sp. biomass could be used as suitable feedstock for biodiesel production.
Cite this paper: Hakalin, N. , Paz, A. , Aranda, D. and Moraes, L. (2014) Enhancement of Cell Growth and Lipid Content of a Freshwater Microalga Scenedesmus sp. by Optimizing Nitrogen, Phosphorus and Vitamin Concentrations for Biodiesel Production. Natural Science, 6, 1044-1054. doi: 10.4236/ns.2014.612095.

[1]   Basu, S., Roy, A.S., Mohanty, K. and Ghoshal, A.K. (2014) CO2 Biofixation and Carbonic Anhydrase Activity in Scenedesmus obliquus SA1 Cultivated in Large Scale Open System. Bioresource Technology, 164, 323-330.

[2]   Converti, A., Casazza, A.A., Ortiz, E.Y., Perego, P. and Del Borghi, M. (2009) Effect of Temperature and Nitrogen Concentration on the Growth and Lipid Content of Nannochloropsis oculata and Chlorella vulgaris for Biodiesel Production. Chemical Engineering and Processing, 48, 1146-1151.

[3]   Ho, S.H., Chen, C.Y. and Chang, J.S. (2012) Effect of Light Intensity and Nitrogen Starvation on CO2 Fixation and Lipid/Carbohydrate Production of an Indigenous Microalga Scenedesmus obliquus CNW-N. Bioresource Technology, 113, 244-252.

[4]   Andruleviciute, V., Makareviciene, V., Skorupskaite, V. and Gumbyte, M. (2014) Biomass and Oil Content of Chlorella sp., Haematococcus sp., Nannochlopsis sp. and Scenedesmus sp. under Mixotrophic Growth Conditions in the Presence of Technical Glycerol. Journal Applied Phycology, 26, 83-90.

[5]   Siaut, M., Cuine, S., Cagnon, C., Fessler, B., Nguyen, M., Carrier, P., Beyly, A., Beisson, F., Triantaphylides, C., Li-Beisson, Y.H. and Peltier, G. (2011) Oil Accumulation in the Model Green Alga Chlamydomonas reinhardtii: Characterization, Variability between Common Laboratory Strains and Relationship with Starch Reserves. BMC Biotechnology, 11, 7.

[6]   Chisti, Y. (2007) Biodiesel from Microalgal. Biotechnology Advances, 25, 294-306.

[7]   Blersch, D.M., Kangas, P.C. and Mulbry, W.W. (2013) Turbulance and Nutrient Interactions That Control Benthic Algal Production in an Engineered Cultivation Raceway. Algal Research, 2, 107-112.

[8]   Tang, D., Han, W., Li, P., Miao, X. and Zhong, J. (2011) CO2 Fixation and Fatty Acid Composition of Scenedesmus obliquus and Chorella pyrenoidosa in Response to Different CO2 Levels. Bioresource Technology, 102, 3071-3076.

[9]   Xin, L., Ying, H.H., Ke, G. and Xue, S.Y. (2010) Effects of Different Nitrogen and Phosphorus Concentrations on the Growth, Nutrient Uptake, and Lipid Accumulation of a Freshwater?Microalga Scenedesmus sp. Bioresource Technology, 101, 5494-5500.

[10]   Liu, J., Huang, J.C., Fan, K.W., Jiang, Y., Zhong, Y.J., Sun, Z. and Chen, F. (2010) Production Potential of Chlorella zofingienesis as a Feedstock for Biodiesel. Bioresource Technology, 101, 8658-8663.

[11]   Mata, T.M., Martins, A.A. and Caetano, N.S. (2010) Microalgae for Biodiesel Production and Other Applications: A Review. Renewable and Sustainable Energy Reviews, 14, 217-232.

[12]   Rawat, I., Ranjith Kumar, R., Mutanda, T. and Bux, F. (2013) Biodiesel from Microalgae: A Critical Evaluation from Laboratory to Large Scale Production. Applied Energy, 103, 444-467.

[13]   Yeh, K.L. and Chang, J.S. (2012) Effects of Cultivation Conditions and Media Composition on Cell Growth and Lipid Productivity of Indigenous Microalga Chlorella vulgaris ESP-31. Bioresource Technology, 105, 120-127.

[14]   Gorham, P.R., Mclachilan, J.R., Hammer, V.T. and Kim, W.K. (1964) Isolation and Culture of Toxic Strains of Anabaena Flos-Aquae (Lyngb.) de Bréb. Verhandlungen der Internationalen Vereinigung für Theoretischeund Angewandte Limnologie, 15, 796-804.

[15]   Bligh, E.G. and Dyer, W.J. (1959) A Rapid Method of Total Lipid Extraction and Purification. Canadian Journal of Biochemistry and Physiology, 37, 911-917.

[16]   Lee, J.Y., Yoo, C., Jun, S.Y., Ahn, C.Y. and Oh, H.M. (2010) Comparison of Several Methods for Effective Lipid Extraction from Microalgae. Bioresource Technology, 101, S75-S77.

[17]   Montgomery, D. and Calado, V. (2003) Experimental Design Using the Statistic. Editorial E-Papers Servi?os Editoriais, Rio de Janeiro.

[18]   Ji, F., Liu, Y., Hao, R., Li, G., Zhou, Y.G. and Dong, R.J. (2014) Biomass Production and Nutrients Removal by a New Microalgae Strain Desmodesmus sp. in Anaerobic Digestion Wastewater. Bioresource Technology, 161, 200-207.

[19]   Zhao, G., Yu, J., Jiang, F., Zhang, X. and Tan, T.W. (2012) The Effect of Different Trophic Modes on Lipid Accumulation of Scenedesmus quadricauda. Bioresource Technology, 114, 466-471.

[20]   Praveenkumar, R., Shameera, K., Mahalakshmi, G., Arbarsha, M.A. and Thajuddin, N. (2012) Influence of Nutrient Deprivations on Lipid Accumulation in a Dominant Indigenous Microalga Chlorella sp., BUM11008: Evaluation for Biodiesel Production. Biomass and Bioenergy, 37, 60-66.

[21]   Mandal, S. and Mallick, N. (2009) Microalga Scenedesmus obliquus as a Potential Source for Biodiesel Production. Applied Microbiology and Biotechnology, 84, 281-291.