effects of nitrogen, phosphorus and vitamin on growth and lipid content of a
freshwater microalgae Scenedesmus sp.
were investigated under indoor conditions. Nitrogen, phosphorus and vitamin
presented significant statistical effect on lipid content, and a maximum lipid
content of 29.3% was obtained, corresponded to increases of 90% when compared
to those obtained with the original nutrients medium. On the other hand, only
nitrogen and phosphorus presented significant statistical effect on cell
growth, achieving ash free dry biomass, algal density, and specific growth rate
of 1.3 g·L-1, 1.5E+07 cells mL-1, and 0.62 d-1,
respectively. By the use of the desirability function, it was possible to
maximize the cell growth and lipid content simultaneously. The fatty acid
composition of the microalgal lipid comprises over 80% of saturated and
monounsaturated fatty acids. Thus, Scenedesmus sp. biomass could be used as suitable feedstock for biodiesel production.
Cite this paper
Hakalin, N. , Paz, A. , Aranda, D. and Moraes, L. (2014) Enhancement of Cell Growth and Lipid Content of a Freshwater Microalga Scenedesmus
sp. by Optimizing Nitrogen, Phosphorus and Vitamin Concentrations for Biodiesel Production. Natural Science
, 1044-1054. doi: 10.4236/ns.2014.612095
 Basu, S., Roy, A.S., Mohanty, K. and Ghoshal, A.K. (2014) CO2 Biofixation and Carbonic Anhydrase Activity in Scenedesmus obliquus SA1 Cultivated in Large Scale Open System. Bioresource Technology, 164, 323-330.
 Converti, A., Casazza, A.A., Ortiz, E.Y., Perego, P. and Del Borghi, M. (2009) Effect of Temperature and Nitrogen Concentration on the Growth and Lipid Content of Nannochloropsis oculata and Chlorella vulgaris for Biodiesel Production. Chemical Engineering and Processing, 48, 1146-1151. http://dx.doi.org/10.1016/j.cep.2009.03.006
 Ho, S.H., Chen, C.Y. and Chang, J.S. (2012) Effect of Light Intensity and Nitrogen Starvation on CO2 Fixation and Lipid/Carbohydrate Production of an Indigenous Microalga Scenedesmus obliquus CNW-N. Bioresource Technology, 113, 244-252. http://dx.doi.org/10.1016/j.biortech.2011.11.133
 Andruleviciute, V., Makareviciene, V., Skorupskaite, V. and Gumbyte, M. (2014) Biomass and Oil Content of Chlorella sp., Haematococcus sp., Nannochlopsis sp. and Scenedesmus sp. under Mixotrophic Growth Conditions in the Presence of Technical Glycerol. Journal Applied Phycology, 26, 83-90. http://dx.doi.org/10.1007/s10811-013-0048-x
 Siaut, M., Cuine, S., Cagnon, C., Fessler, B., Nguyen, M., Carrier, P., Beyly, A., Beisson, F., Triantaphylides, C., Li-Beisson, Y.H. and Peltier, G. (2011) Oil Accumulation in the Model Green Alga Chlamydomonas reinhardtii: Characterization, Variability between Common Laboratory Strains and Relationship with Starch Reserves. BMC Biotechnology, 11, 7. http://dx.doi.org/10.1186/1472-6750-11-7
 Chisti, Y. (2007) Biodiesel from Microalgal. Biotechnology Advances, 25, 294-306.
 Blersch, D.M., Kangas, P.C. and Mulbry, W.W. (2013) Turbulance and Nutrient Interactions That Control Benthic Algal Production in an Engineered Cultivation Raceway. Algal Research, 2, 107-112.
 Tang, D., Han, W., Li, P., Miao, X. and Zhong, J. (2011) CO2 Fixation and Fatty Acid Composition of Scenedesmus obliquus and Chorella pyrenoidosa in Response to Different CO2 Levels. Bioresource Technology, 102, 3071-3076.
 Xin, L., Ying, H.H., Ke, G. and Xue, S.Y. (2010) Effects of Different Nitrogen and Phosphorus Concentrations on the Growth, Nutrient Uptake, and Lipid Accumulation of a Freshwater?Microalga Scenedesmus sp. Bioresource Technology, 101, 5494-5500.