Tolerance of Corn (Zea mays L.) to Early and Late Glyphosate Applications

Kris J.  Mahoney; Robert E.  Nurse; Wesley J.  Everman; Christy L.  Sprague; Peter H.  Sikkema

doi:10.4236/ajps.2014.518291

Kris J. Mahoney, Robert E. Nurse, Wesley J. Everman, Christy L. Sprague, Peter H. Sikkema

Abstract: Fifteen field experiments were conducted from 2009 to 2012 in Ontario, Canada and Michigan, USA to determine the tolerance of corn (Zea mays L.) to early (spike or 1- to 2-leaf stage) or late (8- or 10-leaf stage) applications of 900, 1800, 3600, or 7200 g·ae·ha-1 of glyphosate. Postemergence applications were evaluated for corn injury, cob length and deformity, crop moisture at harvest, and yield in the absence of weed competition. In the early application experiment, no visible injury was detected with applications of up to 3600 g·ae·ha-1; however, 1.4% injury was observed 4 weeks after treatment (WAT) when 7200 g·ae·ha-1 was applied to 1- to 2-leaf stage corn. Yet by harvest, the observed injury was transient as yields were similar to the untreated control regardless of glyphosate dose or timing. In the late application experiment, visible injury tended to increase with glyphosate dose. In addition, for corn treated with 7200 g·ae·ha-1 at the 10-leaf stage, injury increased over time as 6%, 11%, and 12% injury was observed 1, 2, and 4 WAT, respectively. Similar to the visible injury of vegetative tissue, cob deformity and reductions in yield tended to increase with glyphosate dose, but this response varied and the data were pooled into two environment groups. For example, in one environment group, corn treated with 7200 g·ae·ha-1 at the 8- and 10-leaf stage had a 9.5% and 14.6% reduction in yield, respectively. Whereas in another environment group, corn yields were similar to the untreated control regardless of glyphosate dose or timing. This research demonstrated that commercially available corn hybrids have tolerance to glyphosate at doses greater than what has been previously published or could reasonably be expected during spray overlaps in a field.

Keywords: Dose, Injury, Cob Deformity, Yield

Cite this paper: Mahoney, K. , Nurse, R. , Everman, W. , Sprague, C. and Sikkema, P. (2014) Tolerance of Corn (Zea mays L.) to Early and Late Glyphosate Applications. American Journal of Plant Sciences, 5, 2748-2754. doi: 10.4236/ajps.2014.518291.

References

[1] Green, J.M. (2009) Evolution of Glyphosate-Resistant Crop Technology. Weed Science, 57, 108-117.
http://dx.doi.org/10.1614/WS-08-030.1

[2] Pline-Srnic, W. (2006) Physiological Mechanisms of Glyphosate Resistance. Weed Technology, 20, 290-300.
http://dx.doi.org/10.1614/WT-04-131R.1

[3] Pollegioni, L., Schonbrunn, E. and Siehl, D. (2011) Molecular Basis of Glyphosate Resistance—Different Approaches through Protein Engineering. FEBS Journal, 278, 2753-2755.
http://dx.doi.org/10.1111/j.1742-4658.2011.08214.x

[4] Vande Berg, B.J., Hammer, P.E., Chun, B.L., Schouten, L.C., Carr, B., Guo, R., Peters, C., Hinson, T.K., Beilinson, V., Shekita, A., Deter, R., Chen, Z., Samoylov, V., Bryant, C.T., Stauffer, M.E., Eberle, T., Moellenbeck, D.J., Carozzi, N.B., Koziel, M.G. and Duck, N.B. (2008) Characterization and Plant Expression of a Glyphosate-Tolerant Enolpyruvylshikimate Phosphate Synthase. Pest Management Science, 64, 340-345.
http://dx.doi.org/10.1002/ps.1507

[5] Castle, L.A., Siehl, D.L., Gorton, R., Patten, P.A., Chen, Y.H., Bertain, S., Cho, H., Duck, N., Wong, J., Liu, D. and Lassner, M.W. (2004) Discovery and Directed Evolution of a Glyphosate Tolerance Gene. Science, 304, 1151-1154.
http://dx.doi.org/10.1126/science.1096770

[6] Heck, G.R., Armstrong, C.L., Astwood, J.D., Behr, C.F., Bookout, J.T., Brown, S.M., Cavato, T.A., DeBoer, D.L., Deng, M.Y., George, C.Hillyard, J.R., Hironaka, C.M., Howe, A.R., Jakse, E.H., Ledesma, B.E., Lee, T.C., Lirette, R.P., Mangano, M.L., Mutz, J.N., Qi, Y., Rodriguez, R.E., Sidhu, S.R., Silvanovich, A., Stoecker, M.A., Yingling, R.A. and You, J. (2005) Development and Characterization of a CP4 EPSPS-Based, Glyphosate-Tolerant Corn Event. Crop Science, 45, 329-339.
http://dx.doi.org/10.2135/cropsci2005.0329

[7] Soltani, N., Nurse, R.E., Page, E.P., Everman, W.J., Sprague, C.L. and Sikkema, P.H. (2013) Influence of Late Emerging Weeds in Glyphosate-Resistant Corn. Agricultural Sciences, 4, 275-281.
http://dx.doi.org/10.4236/as.2013.46039

[8] US Department of Agriculture—Economic Research Service (2014) Adoption of Genetically Engineered Crops in the U.S.
http://www.ers.usda.gov/data-products/adoption-of-genetically-engineered-
crops-in-the-us/recent-trends-in-ge-adoption

[9] Ontario Ministry of Agriculture, Food and Rural Affairs (2012) Guide to Weed Control. Toronto, Publication 75, 400 p.

[10] Nurse, R.E., Swanton, C.J., Tardif, F. and Sikkema, P.H. (2006) Weed Control and Yield Are Improved When Glyphosate Is Preceded by a Residual Herbicide in Glyphosate-Tolerant Maize (Zea mays). Crop Protection, 25, 1174-1179.
http://dx.doi.org/10.1016/j.cropro.2006.02.015

[11] Soltani, N., Stewart, C.L., Nurse, R.E., Van Eerd, L.L., Vyn, R.J. and Sikkema, P.H. (2012) Weed Control, Environmental Impact and Profitability of Weed Management Strategies in Glyphosate-Resistant Corn. American Journal of Plant Science, 3, 1594-1607.
http://dx.doi.org/10.4236/ajps.2012.311193

[12] Stewart, C.L., Soltani, N., Nurse, R.E., Hamill, A.S. and Sikkema, P.H. (2012) Precipitation Influences Pre- and Post-Emergence Herbicide Efficacy in Corn. American Journal of Plant Science, 3, 1193-1204.
http://dx.doi.org/10.4236/ajps.2012.39145

[13] Anonymous (2011) Roundup WeatherMAX^® Herbicide Product Label. Monsanto Canada Inc., Winnipeg.
http://roundup.ca/_uploads/documents/ru_wmax_label_english.pdf

[14] Hetherington, P.R., Reynolds, T.L., Marshall, G. and Kirkwood, R.C. (1999) The Absorption, Translocation and Distribution of the Herbicide Glyphosate in Maize Expressing the CP-4 Transgene. Journal of Experimental Botany, 50, 1567-1576.
http://dx.doi.org/10.1093/jxb/50.339.1567

[15] Page, E.R., Cerrudo, D., Westra, P., Loux, M., Smith, K., Foresman, C., Wright, H. and Swanton, C.J. (2012) Why Early Season Weed Control Is Important in Maize. Weed Science, 60, 423-430.
http://dx.doi.org/10.1614/WS-D-11-00183.1

[16] Maddonni, G.A. and Otegui, M.E. (2004) Intra-Specific Competition in Maize: Early Establishment of Hierarchies among Plants Affects Final Kernel Set. Field Crops Research, 85, 1-13.
http://dx.doi.org/10.1016/S0378-4290(03)00104-7

[17] Lejeune, P. and Bernier, G. (1996) Effect of Environment on the Early Steps of Ear Initiation in Maize (Zea mays L.). Plant, Cell & Environment, 19, 217-224.
http://dx.doi.org/10.1111/j.1365-3040.1996.tb00243.x

[18] Pline, W.A., Wilcut, J.W., Duke, S.O., Edmisten, K.L. and Wells, R. (2002) Tolerance and Accumulation of Shikimic Acid in Response to Glyphosate Applications in Glyphosate-Resistant and Nonglyphosate-Resistant Cotton (Gossypiumhirsutum L.). Journal of Agricultural and Food Chemistry, 50, 506-512. http://dx.doi.org/10.1021/jf0110699

[19] Thomas, W.E., Pline-Srnic, W.A., Thomas, J.F., Edmisten, K.L., Wells, R. and Wilcut, J.W. (2009) Glyphosate Negatively Affects Pollen Viability But Not Pollination and Seed Set in Glyphosate-Resistant Corn. Weed Science, 52, 725-734.
http://dx.doi.org/10.1614/WS-03-134R