OJGen  Vol.4 No.5 , September 2014
The Genetic Relationship of Vietnamese Pigs in Central Highlands Assessed by Cytochrome b

To estimate the genetic relationship of Vietnamese pigs in Central Highlands, we compared cytochrome b sequences of Vietnamese wild boars and Vietnamese domestic pigs with other Asian and European wild boars. The results showed that there were two wild boar populations locating in Vietnam Central Highlands including wild boars of group I and wild boars of group II. The Vietnamese wild boars of group II and domestic pigs were genetically close to Asian A1 and Asian A2 wild boar groups, whereas the Vietnamese wild boars of group I were genetically distinct from Asian A1, Asian A2 wild boar groups. The phylogenetic tree demonstrated that the Vietnamese wild boars of group I were clustered in one clade which was distinct from Asian wild boars and Europe wild boars. In addition, the Vietnamese wild boars of group I were estimated to have diverged from European wild boars at 421500 YBP, indicating that Vietnamese wild boar of group I could be isolated from other Asian wild boars. The single nucleotide polymorphism analysis showed that three Asian haplotypes were contributed in Vietnamese wild boars including A3 (TATG) haplotype in Vietnamese wild boar of group I and A1 (CATA) haplotype and A2 (CATG) haplotype in Vietnamese wild boars of group II. The A1 haplotype and A2 haplotype were also distributed in Vietnamese domestic pigs. Thus, there is a high possibility that Vietnam Central Highlands is a principal source for research on genetic diversity in Asian wild boar and domestic pig populations.

Cite this paper: Long, L. , Mai, N. , Chung, D. , Si, D. , Chi, H. and Son, H. (2014) The Genetic Relationship of Vietnamese Pigs in Central Highlands Assessed by Cytochrome b. Open Journal of Genetics, 4, 362-369. doi: 10.4236/ojgen.2014.45033.

[1]   Branicki, W., Kupiec, T. and Pawlowski, R. (2003) Validation of Cytochrome b Sequence Analysis as a Method of Species Indentification. Journal of Forensic Sciences, 48, 83-87.

[2]   Clop, A., Amills, M.M., Noguera, J.L., Fernandez, A., Capote, J., Ramon, M.M., Kelly, L., Kijas, J., Andersson, L. and Sanchez, A. (2004) Estimating the Frequency of Asian Cytochrome B Haplotypes in Standard European and Local Spanish Pig Breeds. Genetics Selection Evolution, 36, 97-104.

[3]   Larson, G., Dobney, K., Albarella, U., Fang, M., Smith, E.M., Robins, J., Lowden, S., Finlayson, H., Brand, T., Willerslev, E., Conwy, P.R., Andersson, L. and Cooper, A. (2005) Worldwide Phylogeography of Wild Boar Reveals Multiple Centers of Pig Domestication. Science, 307, 1618-1621.

[4]   Alves, E., óvilo C., Rodríguez, C. and Silió, L. (2003) Mitochondrial DNA Sequence Variation and Phylogenetic Relationships among Iberian Pigs and Other Domestic and Wild Pig Populations. Animal Genetics, 34, 319-324.

[5]   Kim, K.I., Lee, J.H., Li, K., Zhang, Y.P., Lee, S.S., Gongora, J. and Moran C. (2002) Phylogenetic Relationships of Asian and European Pig Breeds Determined by Mitochondrial DNA D-Loop Sequence Polymorphism. Animal Genetics, 33, 19-25.

[6]   Mona, S., Randi, E. and Ponzetta, M.T. (2007) Evolutionary History of the Genus Sus Inferred from Cytochrome b Sequences. Molecular Phylogenetics and Evolution, 45, 757-762.

[7]   Thuy, N.T.D., Melchinger-Wild, E., Kuss, A.W., Cuong, N.V., Bartenschlager, H. and Geldermann, H. (2006) Comparison of Vietnamese and European Pig Breeds Using Microsatellites. Journal of Animal Science, 84, 2601-2608.

[8]   Ishiguro, N., Sasaki, M., Iwasa, M., Shigehara, N., Hongo, H., Anezaki, T., Long, V.T., Lan, D.T.B. and Long, P.T. (2008) mtDNA Variation in Vietnamese pigs, with Particular Emphasis on the Genetic Relationship between Wild Boars from Vietnam and the Ryukyu Islands. Mammal Study, 33, 51-68.[51:MVIVPW]2.0.CO;2

[9]   Wu, G.S., Yao, Y.G., Qu, K.X., Ding, Z.L., Li, H., Palanichamy, M.G., Duan, Z.Y., Li, N., Chen, Y.S. and Zhang, Y.P. (2007) Population Phylogenomic Analysis of Mitochondrial DNA in Wild Boars and Domestic Pigs Revealed Multiple Domestication Events in East Asia. Genome Biology, 8, R245.

[10]   Maniatis, T., Sambrook, J. and Fritsch, E.F. (1982) Molecular Cloning, a Laboratory Manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York.

[11]   Fernández, A.I., Alves, E., Fernández, A., de Pedro, E., López-García, M.A., Ovilo, C., Rodríguez, M.C. and Silió, L. (2008) Mitochondrial Genome Polymorphisms Associated with Longissimus Muscle Composition in Iberian Pigs. Journal of Animal Science, 86, 1283-1290.

[12]   Giuffra, E., Kijas, J.M., Amarger, V., Carlborg, O., Jeon, J.T. and Andersson, L. (2000) The Origin of the Domestic Pig: Independent Domestication and Subsequent Introgression. Genetics, 154, 1785-1791.

[13]   Tamura, K., Peterson, D., Peterson, N., Stecher, G., Nei, M. and Kumar, S. (2011) MEGA5: Molecular Evolutionary Genetics Analysis Using Maximum Likelihood, Evolutionary Distance, and Maximum Parsimony Methods. Molecular Biology and Evolution, 28, 2731-2739.

[14]   Saitou, N. and Nei, M. (1987) The Neighbor-Joining Method: A New Method for Reconstructing Phylogenetic Trees. Molecular Biology and Evolution, 4, 406-425.

[15]   Jones, G.F. (1998) Genetic Aspects of Domestication, Common Breeds and Their Origin. In: Rothschild, M.F. and Ruvinsky, A., Eds., The Genetics of the Pig, CAB International, Wallingford, 17-50.

[16]   Brown, W.M., George, M. and Wilson, A.C. (1979) Rapid Evolution of Animal Mitochondrial DNA. Proceedings of the National Academy of Sciences of the USA, 76, 1967-1971.

[17]   Fernández, A.I., Alves, E., óvilo, C., Rodríguez, M.C. and Silió, L. (2010) Divergence Time Estimates of East Asian and European Pigs Based on Multiple near Complete Mitochondrial DNA Sequences. Animal Genetics, 42, 86-88.

[18]   Lee, C.E. (2002) Evolutionary Genetics of Invasive Species. Trends in Ecology Evolution, 17, 386-391.

[19]   Hongo, H., Ishiguro, N., Watanobe, T., Shigehara, N., Anezaki, T., Long, V.T., Binh, D.V., Tien, N.T. and Nam, N.H. (2002) Variation in Mitochondrial DNA of Vietnamese Pigs: Relationships with Asian Domestic Pigs and Ryukyu Wild Boars. Zoological Science, 19, 1329-1335.