AJAC  Vol.5 No.11 , August 2014
Optimized Assay for Hydrogen Peroxide Determination in Plant Tissue Using Potassium Iodide
Abstract: Here, we present an optimization of colorimetric determination of hydrogen peroxide content in plants using potassium iodide. Our method is based on a one step buffer (extraction and reaction) for the determination of H2O2 in different plant tissues and overcomes interference of soluble antioxidant and color background. A particular attention is paid to buffer pH shown to be tissue dependent. With this inexpensive microplate method, it is possible to analyze 12 experimental samples in about 45 min all in triplicates, with blanks, controls and standard curve.
Cite this paper: Junglee, S. , Urban, L. , Sallanon, H. and Lopez-Lauri, F. (2014) Optimized Assay for Hydrogen Peroxide Determination in Plant Tissue Using Potassium Iodide. American Journal of Analytical Chemistry, 5, 730-736. doi: 10.4236/ajac.2014.511081.

[1]   Foyer, C.H. and Shigeoka, S. (2011) Understanding Oxidative Stress and Antioxidant Functions to Enhance Photo-synthesis. Plant Physiology, 155, 93-100.

[2]   Neill, S., Desikan, R. and Hancock, J. (2002) Hydrogen Peroxide Signalling. Current Opinion in Plant Biology, 5, 388-395.

[3]   Apel, K. and Hirt, H. (2004) Reactive Oxygen Species: Metabolism, Oxidative Stress, and Signal Transduction. Annual Review of Plant Biology, 55, 373-399.

[4]   Lu, S., Song, J. and Campbell-Palmer, L. (2009) A Modified Chemiluminescence Method for Hydrogen Peroxide Determination in Apple Fruit Tissues. Scientia Horticulturae, 120, 336-341.

[5]   Nagaraja, P., Shivakumar, A. and Shrestha, A.K. (2009) Quantification of Hydrogen Peroxide and Glucose Using 3-Me-thyl-2-benzothiazolinonehydrazone Hydrochloride with 10,11-Dihydro-5H-benz(b,f)azepine as Chromogenic Probe. Analytical Biochemistry, 395, 231-236.

[6]   Liu, X. and Zweier, J.L. (2001) A Real-Time Electrochemical Technique for Measurement of Cellular Hydrogen Peroxide Generation and Consumption: Evaluation in Human Polymorphonuclear Leukocytes. Free Radical Biology and Medicine, 31, 894-901.

[7]   Velikova, V., Yordanov, I. and Edreva, A. (2000) Oxidative Stress and Some Antioxidant Systems in Acid Rain-Treated Bean Plants: Protective Role of Exogenous Poly-amines. Plant Science, 151, 59-66.

[8]   Sengupta, D., Guha, A. and Reddy, A.R. (2013) Interdependence of Plant Water Status with Photosynthetic Performance and Root Defense Responses in Vigna radiata (L.) Wilczek under Progressive Drought Stress and Recovery. Journal of Photochemistry and Photobiology B: Biology, 127, 170-181.

[9]   Girotto, E., Ceretta, C.A., Rossato, L.V., Farias, J.G., Tiecher, T.L., De Conti, L., Schmatz, R., Brunetto, G., Schetinger, M.R.C. and Nicoloso, F.T. (2013) Triggered Antioxidant Defense Mechanism in Maize Grown in Soil with Accumulation of Cu and Zn Due to Intensive Application of Pig Slurry. Ecotoxicology and Environmental Safety, 93, 145-155.

[10]   Nounjan, N., Nghia, P.T. and Theerakulpisut, P. (2012) Exogenous Proline and Trehalose Promote Recovery of Rice Seedlings from Salt-Stress and Differentially Modulate Antioxidant Enzymes and Expression of Related Genes. Journal of Plant Physiology, 169, 596-604.

[11]   Noctor, G. and Foyer, C. (1998) Ascorbate and Glutathione: Keeping Active Oxygen under Control. Annual Review of Plant Physiology and Plant Molecular Biology, 49, 249-279.