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 AJPS  Vol.5 No.18 , August 2014
Blueberry: Functional Traits and Obtention of Bioactive Compounds
Abstract: There is pressure from consumers about exchanging synthetic for natural dyes in foods. This review aimed to expose the potential of blueberry (Vaccinium myrtillus) as a source of natural dye, associated with its properties of functional food. Blueberry is a fruit plant natural from temperate climate, rich in phenolic compounds. The properties of phenolic compounds can be attributed to its ability to capture free radicals, deactivating or eliminating them from the body. Anthocyanins are flavonoid phenolic compounds responsible for color of fruits, flowers and vegetables. Blueberry is rich in anthocyanins, being its content more affected by genotype than by climatic or environmental factors. Brazil has the potential to assume an important position in the worldwide market of this fruit, both in natura and as byproducts, whose main destinations are populations from regions with higher income and educational level, as the North America and Europe. Due to the importance of blueberry as a source of natural pigments, as well as its high value while associated with functional food, it is important to improve extraction and stabilization processes of anthocyanins from blueberry fruits. Researches should focus on the technical viability and cost reduction in the extraction of this pigment. Pioneer studies with blueberry have been conducted in Brazil, but the intensification of researches will allow yield maximization, increased levels of anthocyanins in fruits, and compound stability after extraction. Another focus should be the use of these natural pigments in order to replace those currently used.
Cite this paper: Rocha Concenço, F. , Stringheta, P. , Ramos, A. and Terayama Oliveira, I. (2014) Blueberry: Functional Traits and Obtention of Bioactive Compounds. American Journal of Plant Sciences, 5, 2633-2645. doi: 10.4236/ajps.2014.518278.
References

[1]   Griffiths, J.C. (2005) Coloring Foods e Beverages. Food Technology, 59, 38-44.

[2]   Roy, K., Gullapalli, S., Chaudhuri, U.R. and Chakraborty, R. (2004) The Use of Natural Colorant Based on Betalain in the Manufacture of Sweet Products in índia. International Journal of Food Science and Technology, 39, 1087-1091.
http://dx.doi.org/10.1111/j.1365-2621.2004.00879.x

[3]   Streit, N.M., Canterle, L.P., Canto, M.W. and Hecktheuer, L.H.H. (2005) As Clorofilas. Ciência Rural, 35, 748-755.
http://dx.doi.org/10.1590/S0103-84782005000300043

[4]   Silva, J.S. (2000) Secagem e armazenagem de produtos agrícolas. Aprenda Fácil, Viçosa.

[5]   Domingues, A.M., Abadio, F.D.B., Oliveira, V.M., Borges, S.V. and Gay, J. (2002) Caracterização das propriedades físicas do suco de abacaxi (Ananas comosus) em pó desidratado por atomização otimizado através de análise de suporte de superfície de resposta. 18th Congresso Brasileiro de Ciência e Tecnologia de Alimentos, Porto Alegre, August 4-7, 2002, 1717-1720.

[6]   Kluge, R.A., Hoffmann, A. and Bilhalva, A.B. (1994) Comportamento de frutos de mirtilo (Vaccinium ashei Reade) cv. Powder Blue em armazenamento refrigerado. Ciência Rural, 24, 281-285.

[7]   Santos, A.M. (2004) Situação e perspectiva do mirtilo no Brasil. Série Documentos Embrapa CPACT, 285 p.

[8]   Kaur, C. and Kapoor, H.C. (2001) Antioxidants in Fruits and Vegetables—The Millennium’s Health. International Journal of Food Science and Technology, 36, 703-725.
http://dx.doi.org/10.1046/j.1365-2621.2001.00513.x

[9]   Carlson, J.S. (2003) Processing Effects on the Antioxidant Activities of Blueberry Juices. Ph.D. Thesis, Graduate Faculty of North Carolina State University, Raleigh.

[10]   Raseira, M.C.B. and Antunes, L.E.C. (2004) A cultura do Mirtilo (Vaccinium sp.). Série Documentos Embrapa CPACT, 67 p.

[11]   Rocha, F.I.G. (2009) Avaliação da cor e da atividade antioxidante da polpa e extrato de mirtilo (Vaccinium mirtyllus) em pó. M.Sc. Dissertation, Post-Graduation Program in Food Science and Technology, Viçosa.

[12]   Lima, V.L.A.G., Melo, E.A. and Lima, D.E.S. (2002) Fenólicos e Carotenóides Totais em Pitanga. Scientia Agrícola, 59, 447-450.
http://dx.doi.org/10.1590/S0103-90162002000300006

[13]   Brazil. Ministério da Agricultura, Pecuária e Abastecimento (2007) Fruticultura: O setor produtivo da fruticultura.
http://www.agricultura.gov.br

[14]   Embrapa Clima Temperado—EMBRAPA (2009) Sistema de Produção do Mirtilo.
http://www.cnptia.embrapa.br

[15]   Fachinello, J.C. (2008) Mirtilo. Revista Brasileira de Fruticultura, 30, 285-576.
http://dx.doi.org/10.1590/S0100-29452008000200001

[16]   Harry, L., Sartori, S., Bacher, L.B. and Lacerda, M. (2006) Frutas brasileiras e exóticas cultivadas (de consumo “in natura”). Instituto Plantarum, Nova Odessa.

[17]   Eck, P. (1988) Blueberry Science. Rutgers Press, Brunswick.

[18]   Villata, M. (2007) United States Highbush Blueberry Council Announces Best New Blueberry Product Contest Winners. North American Blueberry Council, Folsom.

[19]   Silveira, T.M.T., Raseira, M.C.B., Nava, D.E. and Couto, M. (2011) Blueberry Pollination in Southern Brazil and Their Influence on Fruit Quality. Revista Brasileira de Fruticultura, 33, 81-88.
http://dx.doi.org/10.1590/S0100-29452011005000041

[20]   Antunes, L.E.C. (2002) Amora Preta: Nova Opção de Cultivo no Brasil. Ciência Rural, 32, 151-158.
http://dx.doi.org/10.1590/S0103-84782002000100026

[21]   Silva, S.D.A.E., Antunes, L.E.C., Anthonisen, D.G., Lemões, J.S. and Gonçalves, E.D. (2008) Caracterização de genótipos de mirtilo utilizando marcadores moleculares. Revista Brasileira de Fruticultura, 30, 180-184.
http://dx.doi.org/10.1590/S0100-29452008000100033

[22]   Bianchi, M.L.P. and Antunes, L.M.G. (1999) Radicais livres e os principais antioxidantes da dieta. Revista de Nutrição, 12, 123-130.
http://dx.doi.org/10.1590/S1415-52731999000200001

[23]   Degáspari, C.H. and Waszczynskyj, N. (2004) Propriedades Antioxidantes de Compostos Fenólicos. Visão Acadêmica, 5, 33-40.

[24]   Shahidi, F. and Naczk, M. (1995) Food Phenolics: Sources, Chemistry, Effects and Applications. Technomic Publishing Co., Lancaster.

[25]   Association of Official Analitical Chemistry-AOAC (1984) Official Methods of Analysis of the Association of Official Analytical Chemistry. 12th Edition, Methods no 22058 and 22059, Washington DC.

[26]   Silva, P.C.F. (2003) Propriedades antioxidantes in vitro de uvas branca e de uva tinta e de seus respectivos vinhos elaborados. M.Sc. Dissertation, Federal University of Viçosa, Viçosa.

[27]   Wu, X., Beecher, G.R., Holden, J.M., Haytowitz, D.B., Gebhardt, S.E. and Prior, R.L. (2004) Lipophilic and Hydrophilic Antioxidant Capacities of Common Foods in the United States. Journal of Agricultural and Food Chemistry, 52, 4026-4037.
http://dx.doi.org/10.1021/jf049696w

[28]   Wolfe, K.L., Kang, X., He, X., Dong, M., Zhang, Q. and Liu, R.H. (2008) Cellular Antioxidant Activity of Common Fruits. Journal of Agricultural and Food Chemistry, 56, 8418-8426.
http://dx.doi.org/10.1021/jf801381y

[29]   Sellappan, S., Akoh, C.C. and Krewer, G. (2002) Phenolic Compounds and Antioxidant Capacity of Georgia-Grown Blueberries and Blackberries. Journal of Agricultural and Food Chemistry, 50, 2432-2438.
http://dx.doi.org/10.1021/jf011097r

[30]   Taruscio, T.G., Barney, D.L. and Exon, J. (2004) Content and Profile of Flavanoid and Phenolic Acid Compounds in Conjunction with the Antioxidant Capacity for a Variety of Northwest Vaccinium Berries. Journal of Agricultural and Food Chemistry, 52, 3169-3176.
http://dx.doi.org/10.1021/jf0307595

[31]   Zadernowski, R., Naczk, M. and Nesterowicz, J. (2005) Phenolic Acid Profiles in Some Small Berries. Journal of Agricultural and Food Chemistry, 53, 2118-2124.
http://dx.doi.org/10.1021/jf040411p

[32]   Zheng, W. and Wang, S.Y. (2003) Oxygen Radical Absorbing Capacity of Phenolics in Blueberries, Cranberries, Chokeberries, and Lingonberries. Journal of Agricultural and Food Chemistry, 51, 502-509.
http://dx.doi.org/10.1021/jf020728u

[33]   MAnhita, A.C., Teixeira, D.M. and Costa, C.T. (2006) Application of Sample Disruption Methods in the Extraction of Anthocyanins from Solid or Semi-Solid Vegetables Samples. Journal of Chromatography A, 1129, 14-20.
http://dx.doi.org/10.1016/j.chroma.2006.06.084

[34]   Cooper-Driver, G.A. (2001) Contributions of Jeffrey Harborne and Co-Workers to the Study of Anthocyanins. Phytochemistry, 56, 229-236.
http://dx.doi.org/10.1016/S0031-9422(00)00455-6

[35]   Francis, F.J. (2000) Colorants: Introduction. In: Francis, F.J., Ed., Encyclopedia of Food Science and Technology, Wiley-lnterscience, New York, 380-383.

[36]   Espín, J.C., Soler-Rivas, C., Wichers, H.J. and García-Viguera, C. (2000) Anthocyanin-Based Natural Colorants: A New Source of Antiradical Activity for Foodstuff. Journal of Agricultural and Food Chemistry, 48, 1588-1592.
http://dx.doi.org/10.1021/jf9911390

[37]   Giusti, M.M. and Wrolstad, R.E. (2003) Acylated Anthocyanins from Edible Sources and Their Applications in Food Systems. Biochemical Engineering Journal, 14, 217-225.
http://dx.doi.org/10.1016/S1369-703X(02)00221-8

[38]   Kong, J.M., Chia, L.S., Goh, N.K., Chia, T.F. and Brouillard, F. (2003) Analysis and Biological Activities of Anthocyanins. Review. Phytochemistry, 64, 923-933.
http://dx.doi.org/10.1016/S0031-9422(03)00438-2

[39]   Malacrida, C.R. and Motta, S. (2006) Antocianinas em suco de uva: Composição e estabilidade. Boletim CEPPA, 24, 59-82.

[40]   Bobbio, F.O. and Bobbio, P.A. (1995) Química do processamento de alimentos. Varela, São Paulo.

[41]   Padetec-Parque de Desenvolvimento Tecnológico-Ceará (2009) Células Solares Usando Corantes Fotoexcitáveis.
www.padetec.ufc.br/natucelteoria.htm

[42]   Longo, L. and Vasapollo, G. (2006) Extraction and Identification of Anthocyanins from Smilax aspera L. Berries. Food Chemistry, 94, 226-231.
http://dx.doi.org/10.1016/j.foodchem.2004.11.008

[43]   Bailoni, M.A., Bobbio, P.A. and Bobbio, F.O. (1998) Preparação e estabilidade do extrato antocianico das folhas da Acalipha híspida. Ciência e Tecnolologia de Alimentos, 18, 17-18.
http://dx.doi.org/10.1590/S0101-20611998000100005

[44]   Falcão, L.D., Barros, D.M., Gauche, C. and Bordignon, L.M.T. (2003) Copigmentação intra e intermolecular de antocianinas: Uma revisão. Boletim CEPPA, 21, 351-366.

[45]   Viguera, C.G. and Bridle, P. (1999) Influence of Structure on Colour Stability of Anthocyanins and Flavylium Salts with Ascorbic Acid. Food Chemistry, 64, 21-26.
http://dx.doi.org/10.1016/S0308-8146(98)00107-1

[46]   Dyrby, M., Westergard, N. and Stapelfeldt, H. (2001) Light and Heat Sensitivity of Red Cabbage Extract in Soft Drink Models Systems. Food Chemistry, 72, 431-437.
http://dx.doi.org/10.1016/S0308-8146(00)00251-X

[47]   Sarkis, J.R. (2011) Construção de um sistema de aquecimento ôhmico e sua aplicação no tratamento térmico de polpa de mirtilo. M.Sc. Dissertation, Federal University of Rio Grande do Sul, Porto Alegre.

[48]   Francis, F.J. (1999) Colorants. Eagan Press, Minnesota.

[49]   Simón, G.V., Hernández, R.M.S. and González, M.T.R. (2002) Análisis preliminar de antocianinas en fruto de icaco. Revista Fitotecnia Mexicana, 25, 261-264.

[50]   Pertuzatti, P.B., Jacques, A.C. and Zambiazi, R.C. (2007) Relação de fitoquímicos na casca e polpa de mirtilo (Vaccinium ashei Reade). 16th Congresso de Iniciação Científica, Pelotas, November 27-29, 2007.

[51]   Lohachoompol, V., Mulholland, M., Srzednicki, G. and Craske, J. (2008) Determination of Anthocyanins in Various Cultivars of Highbush and Rabbiteye Blueberries. Food Chemistry, 111, 249-254.
http://dx.doi.org/10.1016/j.foodchem.2008.03.067

[52]   Vendramini, A.L.A. and Trugo, L.C. (2004) Phenolic Compounds in Acerola Fruit (Malpighia punicifolia, L.). Journal of Brazilian Chemical Society, 15, 664-668.
http://dx.doi.org/10.1590/S0103-50532004000500009

[53]   Lima, V.L.A.G., Melo, E.A., Lima, L.S. and Nascimento, P.P. (2000) Flavonóides em Seleções de Acerola (Malpighia sp L.). 1-Teor de Antocianinas e Flavonóides totais. Ciência Rural, 30, 1063-1064.
http://dx.doi.org/10.1590/S0103-84782000000600024

[54]   Mota, R.V. (2006) Caracterização do suco de amora-preta elaborado em extrator caseiro. Ciência e Tecnologia de Alimentos, 26, 303-308.
http://dx.doi.org/10.1590/S0101-20612006000200012

[55]   Santos, M.H. (2007) Influência do processamento e da torrefação sobre a atividade antioxidante do café (Coffea arabica). Química Nova, 30, 604-610.
http://dx.doi.org/10.1590/S0100-40422007000300020

[56]   Sies, H. (1993) Strategies of Antioxidant Defense. European Journal of Biochemistry, 215, 213-219.
http://dx.doi.org/10.1111/j.1432-1033.1993.tb18025.x

[57]   Halliwell, B. and Gutteridge, J.M.C. (1999) Free Radicals in Biology and Medicine. Clarendon Press, New York.

[58]   Dávalos, A., Gomez-Cordovés, C. and Bartolomé, B. (2003) Commercial Dietary Antioxidant Supplements Assayed for Their Antioxidant Activity by Different Methodologies. Journal of Agricultural and Food Chemistry, 51, 2512-2519.
http://dx.doi.org/10.1021/jf021030j

[59]   Pietta, P.G. (2000) Flavonoids as Antioxidants. Journal of Natural Products, 63, 1035-1042.
http://dx.doi.org/10.1021/np9904509

[60]   Magalhães, L.M., Segundo, M.A., Reis, S. and Lima, J.L.F.C. (2008) Methodological Aspects about in Vitro Evaluation of Antioxidant Properties. Analytica Chimica Acta, 613, 1-19.
http://dx.doi.org/10.1016/j.aca.2008.02.047

[61]   Soares, S.E. (2002) ácidos fenólicos como antioxidants. Revista de Nutrição, 15, 71-81.
http://dx.doi.org/10.1590/S1415-52732002000100008

[62]   Brand-Williams, W., Cuvelier, M.E. and Berset, C. (1995) Use of a Free Radical Method to Evaluate Antioxidant Activity. Lebensm Wiss Technology, 28, 25.
http://dx.doi.org/10.1016/S0023-6438(95)80008-5

[63]   Sánchez-Moreno, C., Larrauri, J.A. and Saura-Calixto, F. (1998) A Procedure to Measure the Antiradical Efficiency of Polyphenols. Journal of the Science of Food and Agriculture, 76, 270-276.
http://dx.doi.org/10.1002/(SICI)1097-0010(199802)76:2<270::AID-JSFA945>3.0.CO;2-9

[64]   Wang, S.Y., Chen, C.T., Sciarappa, W., Wang, C.Y. and Camp, M.J. (2008) Fruit Quality, Antioxidant Capacity, and Flavonoid Content of Organically and Conventionally Grown Blueberries. Journal of Agricultural and Food Chemistry, 56, 5788-5794.
http://dx.doi.org/10.1021/jf703775r

[65]   Giovanelli, G. and Buratti, S. (2009) Comparison of Polyphenolic Composition and Antioxidant Activity of Wild Italian Blueberries and Some Cultivated Varieties. Food Chemistry, 112, 903-908.
http://dx.doi.org/10.1016/j.foodchem.2008.06.066

[66]   Prior, R.L., Cao, G.H., Martin, A., Sofic, E., McEwen, J., O’Brien, C., Lischner, N., Ehlenfeldt, M., Kalt, W., Krewer, G. and Mainland, C.M. (1998) Antioxidant Capacity as Influenced by Total Phenolic and Anthocyanin Content, Maturity, and Variety of Vaccinium Species. Journal of the Agricultural and Food Chemistry, 46, 2686-2693.
http://dx.doi.org/10.1021/jf980145d

[67]   Connor, A.M., Luby, J.J. and Tong, C.B.S. (2002) Genotype and Environmental Variation in Antioxidant Activity, Total Phenolic Content, and Anthocyanin Content among Blueberry Cultivars. Journal of the American Society of Horticultural Science, 127, 89-97.

[68]   Rodrigues, E., Poerner, N., Rockenbach, I.I., Gonzaga, L.V., Mendes, C.R. and Fett, R. (2011) Phenolic Compounds and Antioxidant Activity of Blueberry Cultivars Grown in Brazil. Ciência e Tecnologia de Alimentos, 31, 911-917.

[69]   Castrejón, A.D.R., Eichholz, I., Rohn, S., Kroh, L.W. and Huyskenskeil, S. (2008) Phenolic Profile and Antioxidant Activity of Highbush Blueberry (Vaccinium corymbosum L.) during Fruit Maturation and Ripening. Food Chemistry, 109, 564-572.
http://dx.doi.org/10.1016/j.foodchem.2008.01.007

[70]   Kechinski, C.P. (2011) Estudo de diferentes formas de processamento do mirtilo visando a preservação dos compostos antocianicos. Ph.D. Thesis, Federal University of Rio Grande do Sul, Porto Alegre.

[71]   Srivastava, A., Akoh, C.C., Yi, W., Fischer, J. and Krewer, G. (2007) Effect of Storage Conditions on the Biological Activity of Phenolic Compounds of Blueberry Extract Packed in Glass Bottles. Journal of Agricultural and Food Chemistry, 55, 2705-2713.
http://dx.doi.org/10.1021/jf062914w

[72]   Duffy, K.B., Spangler, E.L., Devan, B.D., Guo, Z., Bowker, J.L., Janas, A.M., Hagepanos, A., Minor, R.K., Decabo, R., Mouton, P.R., Shukitt-Hale, B., Joseph, J.A. and Ingram, D.K. (2008) A Blueberry-Enriched Diet Provides Cellular Protection against Oxidative Stress and Reduces a Kainate-Induced Learning Impairment in Rats. Neurobiology of Aging, 29, 1680-1689.
http://dx.doi.org/10.1016/j.neurobiolaging.2007.04.002

[73]   Kalt, W. and Dufour, D. (1997) Health Functionality of Blueberries. HortTechnology, 7, 216-221.

[74]   Kalt, W., Foote, K., Fillmore, S.A.E., Lyon, M., Lunen, T.A.V. and McRae, K.B. (2008) Effect of Blueberry Feeding on Plasma Lipids in Pigs. British Journal of Nutrition, 100, 70-78.
http://dx.doi.org/10.1017/S0007114507877658

[75]   Seeram, N.P., Adams, L.S., Zhang, Y., Sand, D. and Heber, D. (2006) Blackberry, Black Raspberry, Blueberry, Cranberry, Red Raspberry and Strawberry Extracts Inhibit Growth and Stimulate Apoptosis of Human Cancer Cells in Vitro. Journal of Agricultural and Food Chemistry, 54, 9329-9339.
http://dx.doi.org/10.1021/jf061750g

[76]   Boivin, D., Blanchette, M., Barrette, S., Moghrabi, A. and Beliveau, R. (2007) Inhibition of Cancer Cell Proliferation and Suppression of TNF Induced Activation of NFkappaB by Edible Berry Juice. Anticancer Research, 27, 937-948.

 
 
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