AJPS  Vol.5 No.18 , August 2014
In Vitro Germination and Plantlet Establishment of Wild Chamomile of Morocco Cladanthus mixtus (L.) Oberpr. and Vogt

In vitro seeds germination and plantlets establishment of Cladanthus mixtus (L.) Oberpr. and Vogt were studied in this report. A reliable protocol was developed for in vitro seed germination, multiplication and plantlet regeneration of Cladanthus mixtus. The seeds were sterilized and cultured in different media. Among the two basal media evaluated for symbiotic seed germination, gelled distilled water was found to be the best with a high percentage of seeds germination (100%) after 33 hours of culture. Seedlings were further transferred to different types of media. Result observed after 4 weeks showed that MS medium promotes the highest growth with an average of 2.75 ± 0.12 cm shoot length and 2.60 ± 0.29 shoots per explants, and the mean number of roots achieved 3.33 ± 0.17 root per explants with a length of 2.42 ± 0.16 cm. This study showed that macroelements of MS (1962) medium is essential for in vitro shoot multiplication, growth and rooting of shoots of Cladanthus mixtus L.

Cite this paper: Harras, N. and Lamarti, A. (2014) In Vitro Germination and Plantlet Establishment of Wild Chamomile of Morocco Cladanthus mixtus (L.) Oberpr. and Vogt. American Journal of Plant Sciences, 5, 2623-2632. doi: 10.4236/ajps.2014.518277.

[1]   Watson, L.E., Evansb, T.M. and Boluartec, T. (2000) Molecular Phylogeny and Biogeography of Tribe Anthemideae (Asteraceae), Based on Chloroplast Gene ndhF. Molecular Phylogenetics and Evolution, 15, 59-69.

[2]   Oberprieler, C. (2002) A Phylogenetic Analysis of Chamaemelum Mill. (Compositae: Anthemideae) and Related Genera Based upon nrDNA ITS and cpDNA trnL/trnF IGS Sequence Variation. Botanical Journal of the Linnean Society, 138, 255-273.

[3]   Oberprieler, C., Himmelreich, S. and Vogt, R. (2007) A New Subtribal Classification of the Tribe Anthemideae (Compositae). Willdenowia, 37, 89-114.

[4]   Bellakhdar, J. (1997) La pharmacopée marocaine traditionnelle-Médecine arabe ancienne et savoirs populairs. Editions Ibis Press, Paris, XXXXX, 208.

[5]   Greuter, W. (2012) Compositae (pro parte majore). In: Greuter, W. and von Raab-Straube, E., Eds., Compositae. Euro + Med Plantbase—The Information Resource for Euro-Mediterranean Plant Diversity.

[6]   Nègre, R. (1962) Petite flores des régions arides du Maroc. Tome II, 1, 281-282.

[7]   Pottier Alapetite, G. (1981) Flore de la Tunisie. Angiospermes-dicotylédones, Gamopétales. Programme flore et végétation tunisiennes, 655-1190.

[8]   Quezel, P. and Santana, S. (1963) Nouvelle Flore de l’Algérie et des Régions Désertique Méridionales. Editions Centre National de la Recherche Scientifique, Paris, II, 977.

[9]   Jahandiez, E. and Maire, R. (1934) Catalogue des plantes du Maroc. Tomes 1, 2 et 3. Lechevalier, Paris, 913 p.

[10]   Merghoub, N., Benbacer, L., Amzazi, S., Morjani, H. and El Mzibri, M. (2009) Cytotoxic Effect of Some Moroccan Medicinal Plant Extracts on Human Cervical Cell Lines. Journal of Medicinal Plants Research, 3, 1045-1050.

[11]   Franchomme, P., Jollois, R., Pénoël, D. and Mars, J. (1990) L’aromathérapie exactement: Encyclopédie de l’utilisation thérapeutique des huiles essentielles. Roger Jollois Editeur, 44-47.

[12]   Buckle, J. (2003) Basic Plant Taxonomy, Chemistry, Extraction, Biosynthesis, and Analysis (Chapter 3), Clinical aromatherapy. Essential Oils in Practice, 2nd Edition, 38-75.

[13]   Haddad, P.S., Depot, M., Settaf, A., Chabli, A. and Cherrah, Y. (2003) Comparative Study on the Medicinal Plants Most Recommended by Traditional Practitioners in Morocco and Canada. Journal of Herbs, Spices Medicinal Plants, 10, 25-45.

[14]   Benjilali, B., Zrira, S. and Aboulkassem, H. (2005) Plantes Aromatiques et Médicinales, Atouts du Secteur et Exigences Pour Une Valorisation Durable. Edition Actes, Rabat.

[15]   Satrani, B., Ghanmi, M., Farah, A., Aafi, A., Fougrach, H., Bourkhiss, B., Bousta, D. and Talbi, M. (2007) Composition Chimique et Activité Antimicrobienne de l’Huile Essentielle de Cladanthus mixtus. Bienvenue sur le site de la Société de Pharmacie de Bordeaux, 146, 85-96.

[16]   Abdel-Gaber, A.M., Abd-El-Nabey, B.A., Sidahmed, I.M., El-Zayady, A.M. and Saadawy, M. (2006) Inhibitive Action of Some Plant Extracts on the Corrosion of Steel in Acidic Media. Corrosion Science, 48, 2765-2779.

[17]   Gautheret, R.J. (1959) La culture des tissues végétaux. techniques et réalisations. Masson et Cie, Paris, 863 p.

[18]   Côme, D. (1965) L’inhibition de la germination des graines de pommier Pinnus malus L. non dormantes. Rôle possible des phénols tégumentaires. Annales des Sciences Naturelles, 12, 371-478.

[19]   Maliro, M.F.A. and Kwapata, M.B. (2000) Apomictic Embryo Development and Survival in Uapaca kirkiana under in Vitro and in Vivo Seed Germination. Scientia Horticulturae, 83, 139-147.

[20]   Murashige, T. and Skoog, F. (1962) A Revised Medium for Rapid Growth and Bioassays with Tobacco Tissue Cultures. Physiologia Plantarum, 15, 473-497.

[21]   Sahah, R. and Dalal, K.C. (1980) In Vitro Multiplication of Glycyrrhiza. Current Science (India), 49, 69-71.

[22]   Schenk, R. and Hildebrandt, A. (1972) Medium and Techniques for Induction and Growth of Monocotyledonous and Dicotyledonous Plant Cell Cultures. Canadian Journal of Botany, 50, 199-204.

[23]   Badoc, A. (1982) Contribution à l’étude des phénomènes d’organogénèses et de callogenèse de tissue de Fenouil vulgaire (Foeniculum vulgare subsp.capillaceum var. vulgare ( mill.) thellung) cultivés in vitro; analyse des constituants de l’huiles essentielles des explants. D. E. A. Univ. Lille Univ., Lille Flandres Artois, 74 p.

[24]   Gamborg, O.L. and Eveleigh, D.E. (1968) Culture Methods and Detection of Glucanases in Suspension Cultures of Wheat and Barley. Canadian Journal of Biochemistry, 46, 417-421.

[25]   Cozza, R., Turco, D., Bati, C.B. and Bitonti, B. (1997) Influence of Growth Medium on Mineral Composition and Leaf Histology in Micropropagated Plantlets of Olea europaea. Plant Cell, Tissue and Organ Culture, 51, 215-223.

[26]   Leva, A.R., Petruccelli, R., Goretti, R. and Panicucci, M. (1992) Ruolo di alcuni microelementi e carboidrati nella proliferazione “in vitro” di cv. di olivo (Olea europaea L.). Atti Conv. ‘‘Olive oil quality’’, Firenze, 333-334.

[27]   Margara, J. (1989) Bases de la multiplication végétative, Les méristèmes et l’organogenèse. INRA, Paris, 260.

[28]   Fauconnier, M.L., Jaziri, M., Homés, J., Shimomura, K. and Marlier, M. (1996) Anthemis nobilis L. (Roman Chamomile): In Vitro Culture, Micropropagation and the Production of Essential Oils. Biotechnology in Agriculture and Forestry, 37, 16-37.

[29]   Rout, G.R., Samantaray, S. and Das, P. (2000) In Vitro Manipulation and Propagation of Medicinal Plants. Biotechnology Advances, 18, 91-120.

[30]   Asai, I., Yoshihira, K., Omoto, T. and Schimomura, K. (1995) Growth and Essential Oil Production in Shoot Culture and Regenerates of Anthemis nobilis L. Plant Tissue Culture Letters, 12, 303-311.

[31]   Beiderbeck, R. (1982) Tow Phase Culture: One Way to Isolate Lipophilic Substances from Plant Cell Suspension Cultures. Zeitschrift für Pflanzenphysiologie, 108, 27-30.

[32]   Máday, E., Tyihák, E. and Szöke, E. (2000) Occurrence of Formaldehyde in Intact Plants, Micropropagated Plants and Hairy Root Cultures of Chamomile (Matricaria recutita L.). Plant Growth Regulation, 30, 105-110.

[33]   Omoto, T., Asai, I., Ishimaru, K. and Shimomura, K. (1998) Geranyl Isovalerate Accumulation in Adventitious Root Culture of Anthemis Nobilis. Phytochemistry, 48, 971-974.

[34]   Reichling, J., Bisson, W. and Becker, H. (1984) Various Factors Affecting the Accumulation of Essential Oils in Intact Plants Ant in Callus Cultures of Matricaria chamomilla. Planta Medica, 50, 334-337.

[35]   Sato, A., Sharon de Lima, S., Affonso, V.R., Esquilbel, M.A. and Lage, C.L.S. (2006) Micropropagation of Chamomilla recutita (L.) Rauschert: A Shock Treatment Model with Growth Regulators. Scientia Horticulturae, 109, 160-164.

[36]   Echeverrigaray, S., Fracaro, F., Andrade, L.B., Biasio, S. and Atti-Serafini, L. (2000) In Vitro Shoot Regeneration from Leaf Explants of Roman Chamomile. Plant Cell, Tissue and Organ Culture, 60, 1-4.

[37]   Gamborg, O.L., Murashige, T., Thorpe, T.A. and Vasil, I.K. (1976) Plant Tissue Culture Media. In Vitro, 12, 473-478.

[38]   Huang, L. and Murashige, T. (1977) Plant Tissue Culture Media: Major Constituents, Their Preparation and Some Applications. TCA Manual/Tissue Culture Association, 3, 539-548.

[39]   Chand, S., Sahrawat, A.K. and Prakash, D.V.S.S.R. (1997) In Vitro Culture of Pimpinella anisum L. (Anise). Journal of Plant Biochemistry and Biotechnology, 6, 91-95.

[40]   Jha, S. and Sen, S. (1985) Regeneration and Rapid Multiplication of Bowiea volubilis Harv. in Tissue Culture. Plant Cell Reports, 4, 12-14.

[41]   Koblitz, H., Koblitz, D., Schmauder, H.P. and Groger, D. (1983) Studies on Tissue Cultures of the Genus Cinchona L. Alkaloid Production in Cell Suspension Cultures. Plant Cell Reports, 2, 122-125.

[42]   Koblitz, H., Koblitz, H., Schmauder, H.P. and Groger, D. (1983) Studies on Tissue Cultures of the Genus Cinchona L. Plant Cell Reports, 2, 95-97.

[43]   Ravishankar, G.A. and Venkataraman, L.V. (1988) Plant Cell Culture for High Value Metabolites. Science Reporter, 7, 379-384.

[44]   Saxena, C., Rout, G.R. and Das, P. (1998) Micropropagation of Psoralea Corylifolia Linn. Journal of Medicinal and Aromatic Plant Sciences, 20, 8-15.

[45]   Wakhlu, A.K., Nagari, S. and Barna, K.S. (1990) Somatic Embryogenesis and Plant Regeneration from Callus Cultures of Bunium persicum Boiss. Plant Cell Reports, 9, 137-138.

[46]   Zhou, J., Ma, H., Guo, F. and Luo, X. (1994) Effect of Thidiazuron on Somatic Embryogenesis of Cayratia japonica. Plant Cell, Tissue and Organ Culture, 36, 73-79.