Diversity analysis on
secondary metabolites of Antarctic microbes, Rhodococcus sp. NJ-008 and Pseudomonas sp. NJ-011, together with the structural elucidation of some purified compounds,
has been carried out for understanding of their chemical constituents. The
methanol extracts of Rhodococcus sp.
NJ-008 and Pseudomonas sp. NJ-011 were
subjected to HPLC-TOF MS test for diversity analysis on secondary metabolites,
respectively. The chemical constituents of NJ-011 are mainly N-containing
compounds including some alkaloids and short polypeptides, while those of
NJ-008 are not N-containing ones. Three compounds were also isolated and
identified from extract of NJ011 by different column chromatography and
preparative HPLC, and their structures were elucidated as b-carboline (1),
3-benzylhexahydropyrrolo[1,2-a]pyrazine-1,4-dione (2) and 3-isobutylhexahydropyrrolo[1,2-a]pyrazine-1,4-dione
(3) by comparison of TOF MS, 1Hand 13C-NMR data with
those reported. More microbial material of Pseudomonas sp. NJ-011 should be needed for exploration of the minor constituents with
Cite this paper
Wang, C. , Tian, X. , Yang, Q. , Lu, Y. , Ma, L. , Huang, H. and Fan, C. (2014) Diversity of Secondary Metabolites from Two Antarctic Microbes Rhodococcus sp. NJ-008 and Pseudomonas
sp. NJ-011. Open Journal of Marine Science
, 214-220. doi: 10.4236/ojms.2014.43020
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