Back
 OJST  Vol.4 No.7 , July 2014
Osteoclast Response to Bioactive Surface Modification of Hydroxyapatite
Abstract: The aim of this study was to evaluate the in vitro response of osteoclast-like cells (RAW 264.7 cells) to a bioactive hydroxyapatite (HAP) surface that was modified using the 30% phosphoric ac-id-etching procedure reported in our previous paper (2013). The cells on the bioactive HAP surface were multinucleated and were larger than those on the untreated HAP surface. The cell occupancies were greater on the bioactive HAP surface than on the untreated HAP surface at 7, 14, 21, and 28 days of differentiation; in particular, the values at 21 and 28 days were significantly larger (P < 0.05 and P < 0.01, respectively). These findings show that the bioactive HAP surface may enhance the adhesion and differentiation of RAW 264.7 cells as well as osteoblast-like cells, indicating its potential as a superior surface for bone tissue engineering.
Cite this paper: Okazaki, Y. , Abe, Y. , Yasuda, K. , Hiasa, K. and Hirata, I. (2014) Osteoclast Response to Bioactive Surface Modification of Hydroxyapatite. Open Journal of Stomatology, 4, 340-344. doi: 10.4236/ojst.2014.47047.
References

[1]   Hoppe, A., Güldal, N.S. and Boccaccini, A.R. (2011) A Review of the Biological Response to Ionic Dissolution Products from Bioactive Glasses and Glass-Ceramics. Biomaterials, 32, 2757-2774.
http://dx.doi.org/10.1016/j.biomaterials.2011.01.004

[2]   Dorozhkin, S.V. (2010) Bioceramics of Calcium Orthophosphates. Biomaterials, 31, 1465-1485.
http://dx.doi.org/10.1016/j.biomaterials.2009.11.050

[3]   Ito, Y., Tanaka, N., Fujimoto, Y., Yasunaga, Y., Ishida, O., Agung, M. and Ochi, M. (2004) Bone Formation Using Novel Interconnected Porous Calcium Hy-droxyapatite Ceramic Hybridized with Cultured Marrow Stromal Stem Cells Derived from Green Rat. Journal of Biomedical Materials Research Part A, 69, 454-461.
http://dx.doi.org/10.1002/jbm.a.30014

[4]   Morita, K., Doi, K., Kubo, T., Takeshita, R., Kato, S., Shiba, T. and Akagawa, Y. (2010) Enhanced Initial Bone Regeneration with Inorganic Polyphosphate-Adsorbed Hydroxyapatite. Acta Biomaterialia, 6, 2808-2815.
http://dx.doi.org/10.1016/j.actbio.2009.12.055

[5]   Dorozhkin, S.V. (1997) Surface Reactions of Apatite Dissolution. Journal of Colloid and Interface Science, 191, 489-497. http://dx.doi.org/10.1006/jcis.1997.4942

[6]   Dorozhkin, S.V. (1999) Inorganic Chemistry of the Dissolution Phenomenon: The Dissolution Mechanism of Calcium Apatites at the Atomic (Ionic) Level. Comments on Inorganic Chemistry, 20, 285-299.
http://dx.doi.org/10.1080/02603599908021447

[7]   Bertazzo, S., Zambuzzi, W.F., Campos, D.D.P., Ogeda, T.L., Ferreira, C.V. and Bertran, C.A. (2010) Hydroxyapatite Surface Solubility and Effect on Cell Adhesion. Colloids and Surfaces B: Biointerfaces, 78, 177-184.
http://dx.doi.org/10.1016/j.colsurfb.2010.02.027

[8]   Olton, D., Li, J., Wilson, M.E., Rogers, T., Close, J., Huang, L., Kumta, P.N. and Sfeir, C. (2007) Nanostructured Calcium Phosphates (NanoCaPs) for Non-Viral Gene Delivery: Influence of the Synthesis Parameters on Transfection Efficiency. Biomaterials, 28, 1267-1279.
http://dx.doi.org/10.1016/j.biomaterials.2006.10.026

[9]   Meng, S., Zhang, Z. and Rouabhia, M. (2011) Accelerated Osteoblast Mineralization on a Conductive Substrate by Multiple Electrical Stimulation. Journal of Bone and Mineral Metabolism, 29, 535-544.
http://dx.doi.org/10.1007/s00774-010-0257-1

[10]   Abe, Y., Okazaki, Y., Hiasa, K., Yasuda, K., Nogami, K., Mizumachi, W. and Hirata, I. (2013) Bioactive Surface Modification of Hydroxyapatite. Biomed Research International, 2013, Article ID: 626452.
http://dx.doi.org/10.1155/2013/626452

[11]   Suda, T., Takahashi, N. and Martin, T.J. (1992) Modulation of Osteoclast Differentiation. Endocrine Reviews, 13, 66-80.

[12]   Väänänen, H.K., Zhao, H., Mulari, M. and Halleen, J.M. (2000) The Cell Biology of Osteoclast Function. Journal of Cell Science, 113, 377-381.

[13]   Lacey, D.L., Timms, E., Tan, H.L., Kelley, M.J., Dunstan, C.R., Burgess, T., Elliott, R., Colombero, A., Elliott, G., Scully, S., Hsu, H., Sullivan, J., Hawkins, N., Davy, E., Capparelli, C., Eli, A., Qian, Y.X., Kaufman, S., Sarosi, I., Shalhoub, V., Senaldi, G., Guo, J., Delaney, J. and Boyle, W.J. (1998) Osteoprotegerin Ligand Is a Cytokine That Regulates Osteoclast Differentiation and Activation. Cell, 93, 165-176.
http://dx.doi.org/10.1016/S0092-8674(00)81569-X

[14]   Seeman, E. and Delmas, P.D. (2006) Bone Quality—The Material and Structural Basis of Bone Strength and Fragility. The New England Journal of Medicine, 354, 2250-2261.
http://dx.doi.org/10.1056/NEJMra053077

[15]   Wepener, I., Richter, W., van Papendorp, D. and Joubert, A.M. (2012) In Vitro Osteoclast-Like and Osteoblast Cells’ Response to Electrospun Calcium Phosphate Biphasic Candidate Scaffolds for Bone Tissue Engineering. Journal of Materials Science: Materials in Medicine, 23, 3029-3040.
http://dx.doi.org/10.1007/s10856-012-4751-y

 
 
Top