OJAS  Vol.4 No.4 , July 2014
Haematology of Pelophylax ridibundus (Amphibia: Ranidae) of Striata and Maculata Morphs in Populations Living in Conditions of Anthropogenic Pollution in Southern Bulgaria
Abstract: Basic haematological parameters were studied in adult, sexually mature individuals of colour morphs (striata and maculata) in the populations of Pelophylax ridibundus living in biotopes with various degrees of anthropogenic pollution (control, domestic sewage pollution and heavy metal pollution) in Southern Bulgaria. We found that in the polluted biotopes in individuals of both morphs of P. ridibundus the blood parameters: erythrocyte (RBC) and leukocyte (WBS) count, haemoglobin concentration (Hb), haematocrit (PCV) were statistically reliably higher, the number of lymphocytes (Ly) decreased, and the parameters: mean cell haemoglobin (MCH), mean cell haemoglobin concentration (MCHC), mean cell volume (MCV) and differential blood formula changed considerably in comparison with the control group. In terms of anthropogenic pollution, the average values of RBC and Hb were higher in P. ridibundus of striata morph.
Cite this paper: Zhelev, Z. , Arif, M. , Popgeorgiev, G. , Rauf, M. and Mehterov, N. (2014) Haematology of Pelophylax ridibundus (Amphibia: Ranidae) of Striata and Maculata Morphs in Populations Living in Conditions of Anthropogenic Pollution in Southern Bulgaria. Open Journal of Animal Sciences, 4, 206-216. doi: 10.4236/ojas.2014.44026.

[1]   Cabagna M.C., Lajmanovich, R.C., Stringhini, G.A., Sanchez-Hermandes, J.C. and Peltzer, P.M. (2005) Hematological Parameters of Healt Status in the Common Toad Bufo arenarum in the Agroecosystems of Santa Fe Province, Argentina. Applied Herpetology, 2, 373-380.

[2]   Davis, A.K., Maney, D.L. and Maers, J.C. (2008) The Use of Leukocyte Profiles to Measure Stress in Vertebrates: A Review for Ecologist. Functional Ecology, 22, 760-772.

[3]   Korzh, A.P., Zadorozhnyaya, V.Y. and Moseyko, V.V. (2012) Methodological Aspects of Usage of Batrachans Representatives in Bioindicative Researches. Journal of Zaporizhzhya National University, 3, 46-56.

[4]   Lajmanovich, R.C., Peltzer, P.M., Attademo, A.M., Cabagna, M.C. and Junges, C.M. (2012) Los Agroquímicos y Suimpacto en los Anfibios: Un Dilemma de Dificil Solucion. Revista Química Viva, 11, 184-198 (in Spanish).

[5]   Zhelev, Z.M. (2012) Ecology of Tailless Amphibians Living under the Influence of Anthropogenic Factors in Bulgaria. Usage of Some Population Characteristics of Three Common Amphibian Species for the Biomonitoring Purposes. Lambert Academic Publishing, Saarbrucken (in Russian).

[6]   Zhelev, Z.M., Angelov, M.V. andMollov, I.A. (2006) A Study of Some Metric Parameters of the Erythrocytes in Rana ridibunda (Amphibia: Anura) Derived from an Area of Highly Developed Chemical Industry. Acta Zoologica Bulgarica, 58, 235-244.

[7]   Zhelev, Z.M. (2007) Investigation on the Blood Differential Formula in Rana ridibunda (Amphibia: Anura) from the Area of the Maritsa-Istok-1 Steam Power Plant. Acta Zoologica Bulgarica, 59, 181-190.

[8]   Sils, E.A. (2008) Specific of Amphibian (Genus Rana) Peripheral Blood Leucogram under Condition of Anthropogenic Load. In: Ananjeva, N.B., Ed., Problems of Herpetology, Russian Collection Publishing, Saint-Petersburg, 369-374 (in Russian).

[9]   Shutler, D.T. and Marcogliese, D.J. (2011) Leukocyte Profiles of Northern Leopard Frogs, Lithobates pipiens, Exposed to Pesticides and Hematozoa in Agricultural Wetlands. Copeia, 2, 301-307.

[10]   Stojanov, A.J., Tzankov, N. and Naumov, B. (2011) Die Amphibien und Reptilien Bulgariens. Chimaira, Frankfurt am Main.

[11]   Berger, L. and Smielowski, J. (1982) Inheritance of Vertebral Stripe in Rana ridibunda Pall. (Amphibia, Ranidae). Amphibia-Reptilia, 3, 145-151.

[12]   Ishchenko, V.G. and Schupak, E.L. (1974) On the Environmental Differences of Genotypes in Populations of Moor Frogs. Ecology, 4, 93-95 (in Russian).

[13]   Vershinin, V.L. (2005) Amphibian Ecology-Population-Based Approach. Proceeding of the 1th Conference of the Ukrainian Herpetological Society, Kyiv, 10-12 October 2005, 35-39 (in Russian).

[14]   Schwartz, S.S. (1980) Ecological Laws of Environmental Science, Moscow (in Russian).

[15]   Peskova, T.Y. and Zhelev, Z.M. (2009) Comparative Assessment of Population Variability of Marsh Frog Rana ridibunda from Reservoirs in Bulgaria. Problems Contemporary Science and Practice, 3, 16-23 (in Russian, English Summary).

[16]   Piastolova, O.A. and Vershinin, V.L. (1999) Some Cytologic Features of Moor Frogs in the East Ural Radioactive Trace. Ecology, 1, 30-35. (In Russian)

[17]   Sils, E.A. and Vershinin, V.L. (2005) Population Ecology of Amphibians Based on Hematological Parameters. In: Bolshakov, V.N., Ed., Fundamental and Applied Ecology, Ural State University Publishing House, Yekaterinburg, 310-311 (in Russian).

[18]   Zhelev, Z.M., Popgeorgiev, G.S. and Angelov, M.V. (2013) Investigating the Changes in the Morphological Content of the Blood of Pelophylax ridibundus (Amphibia: Ranidae) as a Result of Anthropogenic Pollution and Its Use as an Environmental Bioindicator. Acta Zoologica Bulgarica, 65, 187-196.

[19]   Bannikov, A.G., Darevskii, I.S., Ishtenko, V.G., Rustamov, A.K. and Shterbak, N.N. (1977) Guide Amph. and Rept. USSR. Prosveshtenie Publishing House, Moscow (in Russian).

[20]   Hagström, T. (1973) Identification of Newt Specimens (Urodela triturus) by Recording the Belly Pattern and a Description of Photographic Equipment for Such Registration. British Journal of Herpetology, 4, 321-326.

[21]   Рavlov, D.N., Romanov, M.G., Vasilev, M.K. and Popov, I.C. (1980) Chemical Laboratory Methods. Publishing House Medicine and Physical Culture, Sofia (in Bulgarian).

[22]   Blaxhall, P.C. and Daisley, K.W. (1973) Routine Haematological Methods for Use Fish with Blood. Journal of Fish Biology, 5, 771-781.

[23]   Winthrobe, M.M. (1933) Variations in the Size and Hemoglobin Content of Erythrocytes in the Blood of Various Vertebrates. Folia Haematologie, 51, 32-49.

[24]   Statistica for Windows 2004 Version 7.0. Statsoft Inc., Tulsa.