OJVM  Vol.4 No.7 , July 2014
The Effects of Low Intensity Endurance Activity on Various Physiological Parameters and Exercise Induced Oxidative Stress in Dogs
Abstract: The study of canine athlete physiology has focused on endurance sled dog racing and high intensity short duration Greyhound racing, yet the number of dogs competing in low intensity endurance activities is rising due to the increased numbers of hunting and companion dog activities. There is little information on the physiological effect of longer duration low intensity endurance activities. We set out to evaluate the serum biochemistry, oxidative stress, and cortisol response before and after two consecutive days of exercise in ten healthy unconditioned male dogs. Exercise sessions consisted of 120 minutes on an exercise wheel at 11 km/hour on 2 consecutive days. Blood was collected at four time points: 24 hours pre-exercise (sample 1, Day 0, resting); 2 min post-exercise on days 1 and 2, (samples 2 and 3, respectively); and 20 hours post-exercise, collected on day 3(sample 4). Hematocrit, blood gases, serum chemistry, uric acid, cortisol, and F2-isoprostanes were determined. Serum biochemistry and hematocrit suggested hemoconcentration, mild muscle damage and respiratory alkalosis during exercise, which was expected in the unconditioned canine athlete. In addition, plasma indices of oxidative damage (F2-isoprostanes) increased, as did plasma uric acid (an endogenous antioxidant). Importantly, similar to human studies, plasma F2-isoprostanes decreased 24 hours after exercise suggesting a protective effect of exercise. Serum cortisol concentrations were also markedly elevated at the end of exercise on both days suggesting that timing of sampling may play a role in interpreting cortisol results when looking at previous field studies.
Cite this paper: Huntingford, J. , Levine, C. , Mustacich, D. , Corrigan, D. , Downey, R. and Wakshlag, J. (2014) The Effects of Low Intensity Endurance Activity on Various Physiological Parameters and Exercise Induced Oxidative Stress in Dogs. Open Journal of Veterinary Medicine, 4, 134-144. doi: 10.4236/ojvm.2014.47016.

[1]   Ilkiw, J.E., Davis, P.E. and Church, D.B. (1989) Hematologic, Biochemical, Blood-Gas and Acid Base Values in Greyhounds before and after Exercise. American Journal of Veterinary Research, 50, 583-586.

[2]   Hinchcliff, K.W., Olson, J. and Crusberg, C. (1993) Serum Biochemical Changes in Dogs Competing in a Long Distance Sled Race. Journal of the American Veterinary Medical Association, 202, 401-409.

[3]   Burr, J.R., Reinhart, G.A. and Swenson, R.A. (1997) Serum Biochemical Values in Sled Dogs before and after Competing in Long-Distance Races. Journal of the American Veterinary Medical Association, 211, 175-179.

[4]   Rose, R.J. and Bloomberg, M.S. (1989) Responses to Sprint Exercise In the Greyhound: Effects on Haematology, Serum Chemistry Serum Chemistry and Muscle Metabolites. Research in Veterinary Science, 47, 212-218.

[5]   Piccione, G., Casella, S., Panzera, M., Giannetto, C. and Fazio, F. (2012) Effect of Moderate Treadmill Exercise on Some Physiological Parameters in Untrained Beagle Dogs. Experimental Animals, 61, 511-515.

[6]   Wakshlag, J.J., Stokol, T., Geske, S.M., Gregen, C.E., Angle, C.T. and Gillette, R.L. (2010) Evaluation of Exercise-Induced Changes in Concentrations of C-Reactive Protein and Serum Biochemical Values in Sled Dogs Completing a Long-Distance Endurance Race. American Journal of Veterinary Research, 71, 1207-1213.

[7]   Wakshlag, J.J., Snedden, K. and Reynolds, A.J. (2004) Biochemical and Metabolic Changes Due to Exercise in Sprint-Racing Sled Dogs: Implications for Post-Exercise Carbohydrate Supplements and Hydration Management. Veterinary Therapeutics, 5, 52-59.

[8]   McKenzie, E.C., Jose-Cunilleras, E., Hinchcliff, K.W., Holbrook, T.C., Royer, C., Payton, M.E., Williamson, K., Nelson, S., Willard, M.D. and Davis, M.S. (2007) Serum Chemistry Alterations in Alaskan Sled Dogs during Five Successive Days of Prolonged Endurance Exercise. Journal of the American Veterinary Medical Association, 230, 1486-1492.

[9]   Davenport, G.M., Kelley, R.L. and Altom, E.K. (2001) Effect of Diet on Hunting Performance in English Pointers. Veterinary Therapeutics, 2, 10-23.

[10]   Steiss, J., Ahmad, H.A. and Cooper, P. (2004) Physiologic Response in Healthy Labrador Retrievers during Field Trial Training and Competition. Journal of Veterinary Internal Medicine, 18, 147-151.

[11]   Rovira, S., Munoz, A. and Benito, M. (2008) Effect of Exercise on Physiological, Blood and Endocrine Parameters in Search and Rescue Trained Dogs. Veterinary Medicine, 53, 333-346.

[12]   Baltzer, W.I., Firshman, A.M., Stang, B., Warnock, J.J., Gorman, E. and McKenzie, E.C. (2012) The Effect of Agility Exercise on Eicosanoid Excretion, Oxidant Status, and Plasma Lactate in Dogs. BMC Veterinary Research, 8, 249-257.

[13]   Mastaloudis, A., Leonard, S.W. and Traber, M.G. (2001) Oxidative Stress in Athletes during Extreme Endurance Exercise. Free Radical Biology Medicine, 31, 911-922.

[14]   Hinchcliff, K.W., Reinhart, G.A. and DiSilvestro, R. (2000) Oxidant Stress in Sled Dogs Subjected to Repetitive Endurance Exercise. American Journal of Veterinary Research. 61, 512-517.

[15]   Milne, G.L., Yin, H., Hardy, K.D., Davies, S.S. and Roberts, L.J. (2011) Isoprostane Generation and Function. Chemical Reviews, 111, 5973-5996.

[16]   Dorjgochoo, T., Gao, Y., Chow, W., Shu, X., Yang, G., Cai, Q., Rothman, N., Cai, H., Li, H., Deng, X., Franke, A., Roberts, L.J., Milne, G., Zheng, W. and Dai, Qi. (2012) Major Metabolite of F2-Isoprostane in Urine May Be a More Sensitive Biomarker of Oxidative Stress than Isoprostane Itself. The American Journal of Clinical Nutrition, 96, 405-414.

[17]   Packer, L., Cadenas, E. and Davies, K.J. (2008) Free Radicals and Exercise: An Introduction. Free Radical Biology Medicine, 44, 123-125.

[18]   Sachdev, S. and Davies K.J. (2008) Production, Detection, and Adaptive Responses to Free Radicals in Exercise. Free Radical Biology Medicine, 44, 215-223.

[19]   Gomez-Cabrera, M.C., Domenech, E. and Vina, J. (2008) Moderate Exercise Is an Antioxidant: Upregulation of Anti-oxidant Genes by Training. Free Radical Biology Medicine, 44, 126-131.

[20]   Dunlap, K.E., Reynolds, A.J. and Duffy, L.K. (2006) Total Antioxidant Power in Sled Dogs Supplemented with Blueberries and the Comparison of Blood Parameters Associated with Exercise. Comparative Biochemistry and Physiology—Part A, 143, 429-434.

[21]   Baskin, C.R., Hinchcliff, K.W., Disilvestro, R.A., Reinhart, G.A., Hayek, M.G., Chew, B.P., Burr, J.R. and Swenson, R.A. (2000) Effects of Dietary Antioxidant Supplementation on Oxidative Damage and Resistance to Oxidative Damage during Prolonged Exercise in Sled Dogs. American Journal of Veterinary Research, 6, 886-889.

[22]   Piercy, R.J., Hinchcliff, K.W. and DiSilvestro, R.A., (2000) Effect of Dietary Supplements Containing Antioxidants on Attenuation of Muscle Damage in Exercising Sled Dogs. American Journal of Veterinary Research, 61, 1438-1445.

[23]   Waring, W.S., Convery A., Mishra V., Shenkin A., Webb D.J. and Maxwell S.R. (2003)Uric Acid Reduces Exercise-Induced Oxidative Stress in Healthy Adults. Clinical Science (London), 105, 425-430.

[24]   Angle, C.T., Wakshlag, J.J., Gillette, R.L., Stokol, T., Geske, S., Adkins, T.O. and Gregor, C. (2009) Hematologic, Serum Biochemical, and Cortisol Changes Associated with Anticipation of Exercise and Short Duration High-Intensity Exercise in Sled Dogs. Veterinary Clinical Pathology, 38, 370-374.

[25]   Hennessy, M.B., Davis H.N. and Williams, M.T. (1997) Plasma Cortisol Levels of Dogs at a County Animal Shelter. Physiology & Behavior, 62,485-490.

[26]   Haverbeke, A., Diederich, C., Depiereux, E. and Giffroy, J.M. (2008) Cortisol and Behavioral Response of Working Dogs to Environmental Challenges. Physiology & Behavior, 93, 59-67.

[27]   Taylor, A.W., Bruno, R.S., Frei, B. and Traber, M.G. (2006) Benefits of Prolonged Gradient Separation for High-Performance Liquid Chromatography-Tandem Mass Spectrometry Quantitation of Plasma Total 15-Series F2-Isoprostanes. Analytical Biochemistry, 350, 41-51.

[28]   Taylor, A.W. and Traber, M.G. (2010) Quantitation of Plasma Total 15-Series F2-Isoprostanes by Sequential Solid Phase and Liquid-Liquid Extraction. Analytical Biochemistry, 396, 319-321.

[29]   Rovira, S., Munoz, A. and Benito, M. (2007) Hematologic and Biochemical Changes during Canine Agility Competitions. Veterinary Clinical Pathology, 36, 30-35.

[30]   Willard, M.D. and DiBartola, S. (2000) Disorders of Phosphorus. In: DiBartola, S., Ed., Fluid Therapy in Small Animal Practice, 2nd Edition, W.B. Saunders Company, New York, 163-174.

[31]   Hammel, E.P., Kronfled, D.S., Ganjam, V.K. and Dunlap, H.L. (1977) Metabolic Responses to Exhaustive Exercise in Racing Sled Dogs Fed Diets Containing Medium, Low or Zero Carbohydrate. American Journal of Clinical Nutrition, 30, 409-418.

[32]   Huntingford, J.L., Kirn, B., Cramer, K. and Wakshlag, J.J. (2014) Evaluation of a Performance-Enhancing Supplement in American Foxhounds during Eventing. Journal of Nutritional Science, in press.

[33]   Aktas, M., Auguste, D., Lefebvre, H.P., Toutain, P.L. and Braun, J.P. (1993) Creatine Kinase in the Dog: A Review. Veterinary Research Communications, 17, 353-369.

[34]   Snow, D.H., Harris, R.C. and Stuttard, E. (1988) Changes in Haematology and Plasma Biochemistry during Maximal Exercise in Greyhounds. Veterinary Record, 123, 487-489.

[35]   Reynolds, A.J., Reinhart, G.A., Carey, D.P., Simmerman, D.A., Frank, D.A. and Kallfelz, F.A. (1997) Effect of Protein Intake during Training on Biochemical and Performance Variables Is Sled Dogs. American Journal of Veterinary Research, 60, 789-799.

[36]   Steiss, J.E. and Wright, J.C. (2008) Respiratory Alkalosis and Primary Hypocapnia in Labrador Retrievers Participating in Field Trials in High-Ambient-Temperature Conditions. American Journal of Veterinary Research, 69, 1262-1267.

[37]   Ruggiero, C., Cherubini, A., Ble, A., Bos, A.J.G., Maggio, M., Dixit, V.D., Lauretani, F., Bandinelli, S., Senin, U. and Ferrucci, L. (2006) Uric Acid and Inflammatory Markers. European Heart Journal, 27, 1174-1181.

[38]   Child, R.B., Wilkinson, D.M., Fallowfield, J.L. and Donnelly, A.E. (1998) Elevated Serum Antioxidant Capacity and Plasma Malondialdehyde Concentration in Response to a Simulated Half-Marathon Run. Medicine and Science in Sports and Exercise, 30, 1603-1607.

[39]   Roberts II, L.J. and Milne, G.L. (2009) Isoprostanes. Journal of Lipid Research, 50, S219-S223.

[40]   Ferlazzo, A., Medica, P., Cravana, C. and Fazzio, E. (2009) Endocrine Changes after Experimental Show Jumping. Comparative Exercise Physiology, 6, 59-66.

[41]   Ferlazzo, A., Medica, P., Cravana, C. and Fazzio, E. (2012) Circulating β-Endorphin Adrenocorticotro-
pin, and Cortisol Concentrations of Horses before and after Competitive Show Jumping with Different Fence Heights. Journal of Equine Veterinary Science, 32, 740-746.