MRI  Vol.3 No.3 , August 2014
Proteomic Analysis of Celecoxib on Chondrocytes from Patients with Osteoarthritis
ABSTRACT

Objective: To study a comprehensive proteomic analysis of celecoxib in oseteoarthritis (OA) chondrocytes. Methods: OA chondrocytes were stimulated with celecoxib, IL-1β and IL-1β together with celecoxib. Proteins were extracted from the cells and subjected to 2-dimensional differential image gel electrophoresis (2D-DIGE). Proteins of interest were identified by mass spectrometry. Results: Eighty-six protein spots showed significantly different intensities with each reagent or reagent combination. AAA+ protein, HSP47/Serpin, cAMP-dependent protein kinase type II-beta regulatory subunit, alpha-actin-4 and tubulin decreased with the addition of celecoxib, while apolipoprotein A-V, glutamate carboxipeptide 2, mitochondrial stress-70 protein, sorting nexin-9 and GRP78 increased with the addition of celecoxib. GRP78 is a stress protein and may be chondroprotective. Celecoxib modulated IL-1β stimulated chondrocytes, and CD200R and moesin were identified as such resulting proteins. Conclusion: Protein profiles of OA chondrocytes changed after administration of celecoxib. Further investigation is needed to elucidate the function of each protein in OA chondrocytes.


Cite this paper
Takenouchi, K. , Arito, M. , Sato, T. , Takahashi, K. , Kurokawa, M. , Yudoh, K. , Takai, S. , Kato, T. and Nakamura, H. (2014) Proteomic Analysis of Celecoxib on Chondrocytes from Patients with Osteoarthritis. Modern Research in Inflammation, 3, 90-98. doi: 10.4236/mri.2014.33011.
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