AS  Vol.5 No.8 , July 2014
Search for Molecular Markers of Wheat Resistance to Fungal Pathogens
Abstract: Functional traits, potentially associated with resistance to infection, were investigated in cultivars of bread wheat. Plant responses to infection by hemibiotrophic fungus Stagonospora (Septoria) nodorum were studied under laboratory conditions. Infection-induced up-regulation of genes coding for class III peroxidase, oxalate oxidase and protease inhibitor was greater in leaves with reduced disease symptoms (percentage of chlorotic or necrotic leaf area). Similar association was detected between activity of pectinase inhibitors and disease severity. Resistant cultivar also differed from the susceptible one by increased content of Н2О2 in infected tissues and more intensive deposition of lignin. We discuss possibility of using these functional traits in plant breeding for increased stress tolerance.
Cite this paper: Yarullina, L. , Veselova, S. , Ibragimov, R. , Shpirnaya, I. , Kasimova, R. , Akhatova, A. , Tsvetkov, V. and Maksimov, I. (2014) Search for Molecular Markers of Wheat Resistance to Fungal Pathogens. Agricultural Sciences, 5, 722-729. doi: 10.4236/as.2014.58076.

[1]   Passardi, F., Penel, C. and Dunand, C. (2004) Performing the Paradoxical: How Plant Peroxidases Modify the Cell Wall. Trends in Plant Science, 9, 365-370.

[2]   Almagro, L., Gomez Ros, L.V., Belchi-Navarro, S., Bru, R., RosBarcelo, A. and Pedreno, M.A. (2009) Class III Peroxidases in Plant Defence Reactions. Journal of Experimental Botany, 60, 377-390.

[3]   Berna, A. and Bernier, F. (1999) Regulation by Biotic and Abiotic Stress of a Wheat Germin Gene Encoding Oxalate Oxidase, a H2O2-Producing Enzyme. Plant Molecular Biology, 39, 539-549.

[4]   Liu, G., Sheng, X., Greenshields, D.L., Ogieglo, A., Kaminskyj, S., Selvaraj, G. and Wei, Y. (2005) Profiling of Wheat class III Peroxidase Genes Derived from Powdery Mildew-Attacked Epidermis Reveals Distinct Sequence-Associated Expression Patterns. Molecular Plant-Microbe Interactions, 18, 730-741.

[5]   Minibayeva, F., Kolesnikov, O. and Chasov, A. (2009) Wound-Induced Apoplastic Peroxidase Activities: Their Roles in the Production and Detoxification of Reactive Oxygen Species. Plant, Cell & Environment, 32, 497-508.

[6]   Mika, A., Minibayeva, F., Beckett, R. and Luthje, S. (2004) Possible Functions of Extracellular Peroxidases in Stress-Induced Generation and Detoxification of Active Oxygen Species. Phytochemistry Reviews, 3, 173-193.

[7]   Davoine, C., Le Deunff, E., Ledger, N., Avice, J.C., Billard, J.P., Dumas, B. and Huault, C. (2001) Specific and Constitutive Expression of Oxalate Oxidase during the Ageing of Leaf Sheaths of Ryegrass Stubble. Plant, Cell & Environment, 24, 1033-1043.

[8]   Gavnholta, B. and Larsenb, K. (2002) Molecular Biology of Plant Laccases in Relation to Lignin Formation. Physiologia Plantarum, 116, 273-280.

[9]   Srinivasan, T., Kumar, K.R.R. and Kirti, P.B. (2009) Constitutive Expression of a Trypsin Protease Inhibitor Confers Multiple Stress Tolerance in Transgenic Tobacco. Plant and Cell Physiology, 50, 541-553.

[10]   Kudoyarova, G.R., Farkhutdinov, R.G. and Veselov, S.Yu. (1997) Comparison of the Effects of Nitrate and Ammonium Forms of Nitrogen on Auxin Content in Roots and the Growth of Plants under Different Temperature Conditions. Plant Growth Regulation, 23, 207-208.

[11]   Burkhanova, G.F., Yarullina, L.G. and Maksimov, I.V. (2007) The Control of Wheat Defense Responses during Infection With Bipolarissorokiniana by Chitooligosaccharides. Russian Journal of Plant Physiology, 54, 104-110.

[12]   Adhikari, T.B., Balaji, B., Breeden, J.D. and Goodwin, S.B. (2007) Resistance of Wheat to Mycosphaerella graminicola Involves Early and Late Peaks of Gene Expression. Physiological and Molecular Plant Pathology, 71, 56-68.

[13]   Shpirnaya, I.A., Umarov, I.A., Shevchenko, N.D. and Ibragimov, R.I. (2009) Evaluation of the Activity of Hydrolases and Their Inhibitors Using Substrates Immobilized on Agarose Gel. Applied Biochemistry and Microbiology, 45, 449-453.

[14]   Bindschedler, L.V., Minibaeva, F., Gardner, S.L., Gerrish, C., Davies, D.R. and Bolwell, G.P. (2001) Early Signaling Events in Apoplastic Oxidative Burst in Suspension Cultured French Bean Cells Involve Camp and Ca2+. New Phytologist, 151, 185-194.

[15]   Efetova, M., Zeier, J., Riederer, M., Lee, C.W., Stingl, N., Mueller, M.J., Hartung, W., Hedrich, R. and Deeken, R. (2007) A Central Role of ABA in Drought Stress Protection of Agrobacterium-Induced Tumors on Arabidopsis.
Plant Physiology, 145, 853-862.

[16]   Vierheilig, H., Schweiger, P. and Brundrett, M. (2005) An Overview of Methods for the Detection and Observation of Arbuscular Mycorrhizal Fungi in Roots. Physiologia Plantarum, 125, 393-404.

[17]   Lamb, C. and Dixon, R.A. (1997) The Oxidative Burst in Plant Disease Resistance. Annual Review of Plant Physiology and Plant Molecular Biology, 48, 251-275.

[18]   Gohre, V. and Robatzek, S. (2008) Breaking the Barriers: Microbial Effector Molecules Subvert Plant Immunity. Annual Review of Phytopathology, 46, 189-215.

[19]   Maksimov, I.V., Valeev, A.S., Cherepanova, E.A. and Yarullina, L.G. (2009) Hydrogen Peroxide Production in Wheat Leaves Infected with the Fungus Septoria nodorum Berk. Strains with Different Virulence. Applied Biochemistry and Microbiology, 45, 433-438.

[20]   Caliskan, M., GeTuret, M. and Cuming, A.C. (2004) Formation of Wheat (Triticum aestivun L.) Embryogenic Callus Involved Peroxide-Generating Germin-Like Oxalate Oxidase. Planta, 2004, 132-140.

[21]   Zhou, F., Zhang, Z., Gregersen, P.L., Mikkelsen, J.D., de Neergaard, E., Collinge, D.B. and Thordal-Christensen, H. (1998) Molecular Characterization of the Oxalate Oxidase Involved in the Response of Barley to the Powdery Mildew Fungus. Plant Physiology, 117, 33-41.

[22]   Livingstone, D.M., Hampton, J.L., Phipps, P.M. and Grabau, E.A. (2005) Enhancing Resistance to Sclerotinia Minor in Peanut by Expressing a Barley Oxalate Oxidase Gene. Plant Physiology, 137, 1354-1362.

[23]   Murdoch, L., Corbel, J.C., Reis, D., Bertheau, Y. and Vian, B. (1999) Differential Cell Wall Degradation by Erwinia chrysanthemi in Petiole of Saintpauliaionantha. Protoplasma, 210, 54-74.

[24]   Di Matteo, A., Giovane, A., Raiola, A., Camardella, L., Bonivento, D., De Lorenzo, G., et al. (2005) Structural Basis for the Interaction between Pectin Methylesterase and a Specific Inhibitor Protein. The Plant Cell Online, 17, 849-858.

[25]   Vasyukova, N.I., Chalenko, G.I., Gerasimova, N.G., Balueva, T.A. and Ozeretskovskaya, O.L. (2008) Activation of Elicitor Defensive Properties by Systemic Signal Molecules during the Interaction between Potato and the Late Blight Agent. Applied Biochemistry and Microbiology, 44, 236-240.

[26]   Plotnikova, L.Y. (2009) Participation of Reactive Oxygen Species in Protection Wheat Lines with Genes Stability of Species Agropyron Brown Rust. Russian Journal of Plant Physiology, 56, 200-209.

[27]   Mur, L.A.J., Kenton, P., Atzorn, R., Miersch, O. and Wasternack, C. (2006) The Outcomes of Concentration-Specific Interactions between Salicylate and Jasmonate Signaling Include Synergy, Antagonism, and Oxidative Stress Leading to Cell Death. Plant Physiology, 140, 249-262.

[28]   Troshina, N.B., Maksimov, I.V., Yarullina, L.G., Surina, O.B. and Cherepanova, E.A. (2004) Effect of Salicylic Acid to Generate Hydrogen Peroxide in the Wheat Calli Cells Infected by Smutpathogen. Tsitologiia, 46, 1001-1005.

[29]   Boudet, A.M. (2000) Lignins and Lignification: Selected Issues. Plant Physiology and Biochemistry, 38, 81-96.